Advanced methods in fluorescence microscopy

advertisement
Advanced methods in fluorescence microscopy
Theory and basics of fluorescence microscopy and imaging
Introduction to life science research applications
Fluorescence microscopy of living cells.
Date:
Time:
Place:
Speaker:
1st July 2011
10.00 am – 12.30 pm
Seminarraum, Otto Hahn Platz 9 (Biochemie Neubau, EG)
24098 Kiel
Dr. Helge Schmidt (Olympus Deutschland GmbH)
Topics:

Nature of light: What is resolution? What are airy discs?

Shining fluorescence: Why use fluorescence?

Requirements for a fluorescence microscopy system

Physical limits and methods to overcome: What exactly is the point
spread function? What causes stray light effects?

How to overcome stray light effects: The confocal principle,
deconvolution, TIRFM

Overview special applications: FRET, FRAP, FLIP, FLIM, etc…

Live Cell Imaging
Download
Related flashcards
Blood proteins

21 Cards

Animal cells

11 Cards

Proteins

78 Cards

Enzymes

92 Cards

Create flashcards