Advanced methods in fluorescence microscopy
Theory and basics of fluorescence microscopy and imaging
Introduction to life science research applications
Fluorescence microscopy of living cells.
Date:
Time:
Place:
Speaker:
1st July 2011
10.00 am – 12.30 pm
Seminarraum, Otto Hahn Platz 9 (Biochemie Neubau, EG)
24098 Kiel
Dr. Helge Schmidt (Olympus Deutschland GmbH)
Topics:
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Nature of light: What is resolution? What are airy discs?
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Shining fluorescence: Why use fluorescence?
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Requirements for a fluorescence microscopy system
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Physical limits and methods to overcome: What exactly is the point
spread function? What causes stray light effects?
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How to overcome stray light effects: The confocal principle,
deconvolution, TIRFM
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Overview special applications: FRET, FRAP, FLIP, FLIM, etc…
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Live Cell Imaging