LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – November 2008
ED 24
BT 1820 - ADVANCED BIOCHEMISTRY
Date : 06-11-08
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
SECTION – A
Answer all the questions
(20 marks)
I. Choose the best answer.
1.
Glucose and fructose are
a)isomers b)epimers
2.
(5 X 1 = 5)
c)anomers
d)stereoisomers
Which of the following is an essential fatty acid
a)stearic acid b)loeic acid c)arachdonic acid d)palmitic acid
3.
Ammonia is excreted out in the form of
a)urea b)uric acid c)ammonia d)uriamide
4.
An example of a basic aminoacid
a)Valine b) Leucine c) Arginine d) Isoleucine
5.
Non protein part of any enzyme is called
a) apoenzyme b) epienzyme c) coenzyme d)holoezyme
II State whether the following are true or false, if false state reason.
6.
Liver is the storage organ for glucose.
7.
Peptide bonds is broken during denaturation of proteins.
8.
Amino acids are regarded as ampholytes.
9.
Preproinsulin is the precursor molecule for the active insulin.
(5 X 1 = 5)
10. Uric acid has been implicated in the gout disorder.
III.Complete the following.
(5 X 1 = 5)
11.
The only amino acid which is optically inactive is--------------.
12.
Insulin is produced by------------------.
13.
Process of synthesis of glucose from non carbohydrate source is called--------------.
14.
An example of an antioxidant is --------------------------.
15.
Niacin deficiency results in a condition called----------------------.
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IV.Answer the following each within 50 words each
16.
17.
18.
19.
20.
(5 X 1 = 5)
Explain the regulatory action of hexokinase .
Define Isoelectric pH .
What are Zwitter ions?
Give the structure of any two basic amino acids.
Differentiate between LDL and HDL
PART - B
Answer any five questions,each within 350 words only
21.
22.
23.
24.
25.
26.
27.
28.
(5x8=40)
Explain the principle of Bio-energetics .
Write a note on classification of amino acids .
Write a short note on the Kreb`s Cycle .
Explain the steps involved in β-oxidation of fatty acids.
Define the term electrophoresis and give its principle and application.
What are free radicals? How are they generated and explain their source .
Describe the properties of acids and bases and add a note on Arrhenius theory.
Explain the bio-synthesis and regulation of Insulin.
PART C
Answer the following, each within 1500 words:
(2x20=40)
29. a). Write a detailed answer on the classification, physical and chemical reactions on
amino acids.
( Or )
b). Describe the steps involved in the breakdown of 1 glucose molecule to yield 36
ATP molecules.
30. a). Give an account on the two major pathways in nucleotide metabolism.
( Or )
b). Discuss in detail the methods of separation and purification of proteins.
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