LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2012
BT 1826 - BIOCHEMISTRY & BIOPHYSICS
Date : 05/11/2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART –A (20 Marks)
ANSWER ALL THE QUESTIONS
I. Choose the best answer:
1)
(5x1= 5)
Reduction of molecules involves:
a. Loss of energy b. Gain of Oxygen c. Loss of Hydrogen d. Gain of electrons.
2)
Which of the following is not true about peptide bonds?
a. Planar
3)
c. Rigid
d. Weak
Which of the following compound is not a substrate for gluconeogenesis pathway?
a. Glycerol
4)
b. Partial double-bond character
b. Lactate
c. Glycogen
d. Oxaloacetate
Which of the following methods is used to separate molecules based on their size :
a. Ion exchange b. IEF c. Differential centrifugation d. PET
5)
What is the wavelength of electromagnetic radiation used for protein crystallography?
a. 1nm
b. 0.1nm c. 10 nm
d. 100nm
II. State whether the following are true or false; if false give reason:
6)
7)
8)
9)
10)
Water expands on freezing
Proteins give more energy than lipids.
Metabolic control is not required for homeostasis.
MRI images brain using radionuclides.
The binding affinity of ligand is lowered by lowering the pH.
III. Complete the following:
11)
12)
13)
14)
15)
(5x1= 5)
(5x1= 5)
The bond angle of water molecule is __________.
______________ amino acid has an imino ring.
Gout is caused by increased ___________.
The UV light range in the electromagnetic spectrum is from _____ to ______.
The Bragg’s law is given by the equation ______________.
IV. Answer the following, each within 50 words:
(5x1= 5)
16)
17)
18)
19)
20)
State the zeroth law of thermodynamics.
What are enantiomers?
Give the name of any one disorder associated with lipid metabolism.
Define Rf.
What is the principle behind electrophoresis?
PART-B
V. Answer the following, each within 500 words; Draw diagrams wherever necessary:
21)
(5x8=40)
a. Mention any eight properties of water.
(OR)
b. Write short notes on
i) Henderson Hasselbalch equation
ii) Ion product of water.
22)
a. Draw the structures of any eight amino acids.
(OR)
b. Make a table of vitamins A, D, C and their functions.
23)
a. Compare any two metabolic disorders.
(OR)
b. Provide the steps involved in substrate level phosphorylation in Glycolysis.
24)
a. Explain about preparative centrifugation techniques.
(OR)
b. Discuss the in vivo imaging techniques available for studying brain.
25)
a. Comment on the principle and requirements of isoelectric focusing.
(OR)
b. Why is crystallography used as the most versatile tool in structure analysis?
PART-C
VI. Answer any two of the following, each within 1500 words; Draw diagrams wherever necessary
(2x20= 40)
26) Describe pH and pH scale and the working of a pH meter.
27) Elaborate on the classification of carbohydrate and lipid with structures.
28) Give a detailed account on the synthesis and degradation of fatty acids.
29) Review the separation of biological molecules based on mass and charge.
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