BIOTECHNOLOGY SYLLABUS
SCHEME OF INSTRUCTION AND EXAMINATION
B.Tech. (Biotech) – III YEAR -I Semester
SUBJECT
CODE
SUBJECTS
PERIODS/
WEEK
DURATION
OF EXAM
MARKS
The / Lab
Sessional
Exams.
Hrs.
BTT.3.101
Protein Engineering and
Enzyme Technology
4
30
70
3
BTT.3.102
Bio-fluid Mechanics and
Heat Transfer
4
30
70
3
BTT.3.103
Probability and
Statistics
4
30
70
3
BTT.3.104
Plant and Animal
Biotechnology
4
30
70
3
BTT.3.105
Bio-reaction Engineering
4
30
70
3
BTT.3.106
Fermentation
Technology
4
30
70
3
BTP.3.107
Bio-reaction Engineering
Lab
4
25
50
4
BTP.3.108
Fluid Mechanics and
Heat Transfer Lab
4
25
50
4
BTP.3.109
Fermentation Lab
4
25
50
4
255
570
ROTEIN ENGINEERING & ENZYME TECHNOLOGY
Subject code
: BTT.3.101
Sessional
: 30
Periods / Week : 4
Examination
: 70
Nature of Examination : Theory
Exam Duration
: 3 Hrs
UNIT- I PROTEIN STRUCTURE AND FUNCTIONS
Peptide bond- Structure, functions, physical and chemical properties, chemical synthesis of
peptides; liquid phase and solid phase techniques; Proteins-classification & Biological functions;
Physico-chemical properties, forces stabilizing protein structure-primary structure and its
determination, helical-p-pleated structure; Ramachandran plot; super secondary structure, tertiary
& quaternary structure; myoglobin and hemoglobin, Lysozyme, Ribonuclease A, Carboxy
Peptidase, Chymotrypsin, Sickle cell hemoglobin; structure and functional relationship; Fibrous
protein (Collagen).
UNIT-II PROTEIN BIOSYNTHESIS AND STRUCTURE PREDICTION
Methods of protein isolation, purification and Quantification; large scale synthesis of proteins,
design and synthesis of peptides, use of peptides in biology, methods of detection and analysis of
proteins; examples of engineered proteins, protein design, and examples. Post translation
modification, protein transport, Inhibitors of Protein Synthesis. Random, site directed catalytic
affectivity; Structure prediction and modeling of proteins:
UNIT- III PRODUCTION AND APPLICATIONS OF ENZYMES
Production and purification of crude Enzyme extracts from plant, animal and microbial sources;
Methods of characterization of enzymes; development of enzymatic assays. Production of
recombinant proteins (Insulin, Interleukin, Interferon) having therapeutic and diagnostic
applications; Important commercial enzymes; Proteases; Amylases; Lipases; Cellulases;
Pectinases; Isomerases and its applications in food, pharmaceutical and other industries;
Enzymes for analytical and diagnostic purposes; Design of enzyme electrodes and their
application as biosensors in industry health care and environment.
UNIT- IV MECHANISMS AND KINETICS OF ENZYME ACTION
Mechanisms of enzyme action; Concept of active site and energetics of enzyme substrate
complex formation; Specificity of enzyme action; Kinetics of single substrate reactions; Turn
over number; Estimation of Michaeli-Menten parameters; Multi substrate reaction mechanisms
and kinetics; Types of inhibition - Allosteric regulation of enzymes; Deactivation of kinetics.
UNIT- V ENZYME IMMOBILIZATION & MASS TRANSFER EFFECTS IN
IMMOBILISED ENZYME SYSTEMS
Physical and chemical techniques for enzyme immobilization - adsorption, matrix entrapment,
encapsulation, cross-linking, covalent binding etc., examples; Advantages and disadvantages of
different immobilization techniques; Overview of applications of immobilized enzyme systems;
Applications of enzymes in analysis; Analysis of Film and pore Diffusion Effects on kinetics of
Immobilized Enzyme Reactions; Formulation of dimensionless groups and calculation of
Effectiveness Factors.
EXAMINATION:
One question from each unit with internal choice
TEXT BOOKS:
1. Principles of Biochemistry- Lenhinger, David Nelson, W.H.Freeman, (2006).
2. Enzyme Technology- Palmer Trevor, E.W.P, (2004).
3. Fundamentals of Biochemistry by J.L. Jain, Chand (S.) & Co Ltd , India ((1999)).
REFERENCE BOOKS:
1. Biochemistry- Voet and Voet J.G, 2nd edition, John C.Wiley & Sons (1994).
2. Biochemical Engineering James M. Lee eBook Version 2.2. ii © (2001) by James M.
Lee, Gerald Reed , Steve Taylor Third Edition, Academic Press.
3. Enzymes by Paul R. Mathewson Eagan Press Handbook Series (1998).
4. Biocatalysis - Fundamentals and Applications Edited by Bommarius, Andreas Sebastian;
Riebel, Bettina R. Wiley-VCH (2004).
5. Biocatalysts and Enzyme Technology by Klaus Buchholz, Volker Kasche, Uwe Theo
Bornscheuer, Wiley (2005).
6. Enzyme Technology by Ashok Pandey, Collins and others, Springer, (2006).
BIO-FLUID MECHANICS AND HEAT TRANSFER
Subject code
: BTT.3.102
Sessional
: 30
Periods / Week : 4
Examination
: 70
Nature of Examination : Theory
Exam Duration
: 3 Hrs
UNIT-I
Basic Concepts in Flow of Fluids: Introduction, Nature of fluid, Rheology of fluids -Newton's
law of viscosity. Concept of Newtonian and non-Newtonian fluids- Different types of nonNewtonian fluids with examples in bioprocessing. Measurement of viscosity using extrusion
rheometer, plate and cone viscometer, coaxial cylinder viscometer etc.
UNIT-II
Flow field, Friction losses in laminar flow through a circular tube (Hagen-Poiseuille equation),
Friction losses in turbulent flow (Fanning equation), Pumping of fluids Flow through pipes,
average velocity, flow regimes, boundary layer concept. Laminar and turbulent flow characterization by Reynold's number, pressure drop due to skin friction and form friction,
friction factor chart, Hageri -Poiseuille equation.
UNIX-III
Flow past immersed bodies: Definition of drag and drag coefficient. Friction in flow through
beds of solids, Brief introduction to flow of compressible fluids. Flow measuring and monitoring
systems- valves, bends, elbows, prevention of leaks, mechanical seals, stuffing box. Flow
measuring devices-manometers, orifice meter, venture meter and rotameter. Brief description of
Pumps and Blowers
UNIT-IV
Basic Concepts in "Heat Transfer: Introduction and Mechanisms of heat transfer; Conduction
heat transfer (through slab, cylinder & Sphere); Conduction through solids in series, Forced
convection heat transfer inside pipes, Introduction to radiation heat transfer, Chilling and
freezing of food and Biological materials. Heat .transfer correlations, and calculations, basic heat
exchange equipment
UNIT-V
Basic Concepts in Evaporation and Condensation: Introduction, Types of evaporation equipment
and operation methods; Overall heat transfer coefficients in evaporators; simple material
balances. Calculation methods for single effect evaporators, Evaporation of biological materials.
Types of condensation, numerical problems and condensation equipment.
EXAMINATION:
One question from each unit with internal choice
TEXT BOOKS:
1. Unit operations in Chemical Engineering, W L McCabe & J C Smith, 6th Ed., McGraw Hill
Intl. Ed.(2005).
2 Transport Processes and Unit Operations by Christie J. Geankoplis,
India Pvt. Ltd.
3rd Ed., Prentice Hall
3. “Basic Fluid Mechanics”, Kothandaraman C P and Rudramoorthy. R., New Age International
Publishers, New Delhi, (1998).
4. “Fundamentals of Engineering Heat and Mass Transfer” by Sachdeva. R.C., New Age
International Publishers. New Delhi, (1996)
REFERENCE BOOKS:
1. Introduction to Chemical Engineering by S. K. Ghosal, S. K. Sanyal and S. Dutta, TMH
publications, (1993).
2. Computational Fluid Mechanics and Heat Transfer, by Richard Pletcher Second Edition,
Pub: Taylor & Francis; (1997)
3. "Mechanics of Fluids". By Irving H Shames. 3rd Ed., McGraw-Hill Publishing Co.. New
York, (1992).
4. “Rate Processes in Metallurgy”, Mohanty A.K., Prentice Hall of India, New Delhi,
(2000)
5. “Transport Phenomena “by Byron Bird R., Stewart.W.E., John-Wiley & Sons Inc.,
(1994).
6. Engineering Thermofluids: Thermodynamics, Fluid Mechanics, and Heat Transfer By
Mahmoud Massoud Publisher: Springer (2005).
7. Introduction To Thermal Systems Engineering: Thermodynamics, Fluid Mechanics And
Heat Transfer, by Michael J. Moran, Howard N. Shapiro, Bruce R. Munson, David P.
DeWitt, John Wiley & Sons edition (2003).
8. Fundamentals of Fluid Mechanics by Bruce R. Munson, Theodore H. Okiishi, Donald F.
Young, John Wiley & Sons Inc, (2005).
9. Fluid Mechanics with Engineering Applications by E. John Finnemore, Joseph B.
Franzini, McGraw-Hill College (2001).
10. Introduction to Thermal Systems Engineering:Thermodynamics, Fluid Mechanics, and
Heat Transfer by Michael J. Moran, Bruce R. Munson, Howard N. Shapiro, David P.
Dewitt , John Wiley & Sons Inc, (2002).
PROBABILITY AND STATISTICS
Subject code
: BTT.3.103
Sessional
: 30
Periods / Week : 4
Examination
: 70
Nature of Examination : Theory
Exam Duration
: 3 Hrs
UNIT-1 PROBABILITY AND PROBABILITY DISTRIBUTIONS
Probability: Sample space and events - counting - probability - The Awicens of probability some elementary theorems - conditional probability - mathematical; Probability Distributions:
Random variables - The Binomial Distribution- The mean and the Variance of a probability
Distribution - Chebyshev's theorem - The Poisson approves - motion of the binomial distribution
- Poisson procession.
UNIT -II PROBABILITY DENSITIES AND SAMPLING DISTRIBUTIONS
Probability Densities: Continuous Random Variables - The normal Distribution - The normal
Approximation to the Binomial Distribution. Sampling Distributions: Populations and Samples;
The sampling Distribution of the Mean (known) - The sampling distribution of the Mean
(unknown) - The sampling Distribution of the Variance.
UNIT -III INTERFERENCE
Interference Concerning Means: Point Estimation - Interval Estimation - Bayesians Estimation Tests of Hypothesis and Significant Tests - Hypothesis concerning one mean - Inferences
concerning two means; Interference concerning proportions: Estimation of proportions;
Hypothesis concerning one proportion.
UNIT IV CURVE FITTING
Curve fitting; The method of least squares - Inferences based on the least squares estimators curve linear regression- Multiple regression -correlation -Multiple linear regression.
UNIT V BIOSTATISTICS
Statistical basis of biological assays: Response-Dose Metameter, Delusion Assays, direct and
Indirect Assays, Standard line Interpolation assay, Parallel line Assay (4 Point and 6 Point
Assays) and Slope Ratio Assay; Statistical Quality Control Charts and Application of Statistical
Concepts in Biological Sciences.
EXAMINATION:
One question from each unit with internal choice
TEXT BOOKS:
1. Miller & Freud's probability and statistics for Engineers, 5th ed., Richard A. Johnson, PHI.
2. Probability and statistics for engineers. R. Miller, J. E.Freund, and R. Johnson. Prentice Hall,
(1990).
3. Biostatistics: A Foundation for Analysis in the Health Sciences (Wiley Series in Probability
and Statistics), by Wayne W. Daniel, Wiley; 7TH edition (1999).
REFERENCE BOOKS:
1. Probability and Statistics, M.R. Spiegel, Schaum Series, Tata McGraw-Hill, (2007) 9th RP.
2 Statistics for Biologists, by Campbell, R.C., Cambridge University Press
3.Introductory Probability and Statistical Applications- K. Paul Meyer.
4. Biometry: The Principles and Practice of Statistics in Biological Research
by Robert R. Sokal , F. James W.H. Freeman & Company; 2nd edition(1994).
5. Biostatistical Analysis (5th Edition) by Jerrold H. Zar Publisher: Prentice Hall;
(2009).
6. Essentials of Statistics (3rd Edition) Addison Wesley (2006)
7. Experimental Design and Data Analysis for Biologists by Gerry P. Quinn, Michael J. Keough.
Cambridge University Press; 1st Edition (2002)
8. Biostatistics: A Methodology for the Health Sciences (Wiley Series in Probability &
Mathematical Statistics: Applied Probability & Statistics) Wiley-Interscience (1996)
9. Accelerated Testing: Statistical Models, Test Plans, and Data Analysis (Wiley Series in
Probability and Statistics Wiley-Interscience February (1990)
10. Statistical Methods for Reliability Data (Wiley Series in Probability and Statistics)
Wiley-Interscience; 1st Edition (1998).
PLANT AND ANIMAL BIOTECHNOLOGY
Subject code
: BTT.3.104
Sessional
: 30
Periods / Week : 4
Examination
: 70
Nature of Examination : Theory
Exam Duration
: 3 Hrs
UNIT I TISSUE CULTURE
Introduction to cell and tissue culture; Tissue culture media (composition, preparation); Initiation
and maintenance of callus and cell suspension culture, organogenesis; Protoplast isolation,
culture and fusion.
UNIT II TISSUE CULTURE IN CROP IMPROVEMENT
Micropropagation for virus-free plants, Somaclonal variation, Somatic Hybridization, Haploids
in plant breeding, Germplasm conservation (Cryopreservation).
UNIT III IN VITRO PRODUCTION OF SECONDARY METABOLITES.
Production of secondary metabolites from plant cell cultures; Processes for enhancing the
production of secondary metabolites. Technology of plant cell culture for production of
chemicals; Bioreactors systems and models for mass cultivation of plant cells
UNIT-IV ANIMAL CELL TISSUE CULTURE
History and scope of animal cell tissue culture. advantages and disadvantages of tissue culture;
laboratory facilities for animal tissue culture; the substrate on which cells grow; treatment of
substrate surfaces. Feeder layers on substrates. The gas phase for tissue culture. Culture media
for cells and tissues. Cell culture procedures. Tissue culture Slide, Flask and test tube cultures,
Organ culture, Whole embryo culture.
UNIT-V CELL LINES, CLONING AND GENE TRANSFER
Disaggregating (Enzymatic and Mechanical) of tissue and Primary culture. Culture cells and
evolution of cell lines. Maintenance of cultures- Cell lines. Cloning of cell lines. Large scale
cultures in Biotechnology. Somatic cell fusion. Gene transfer or Transfection (using Eggs and
cultured stem cells); targeted gene transfer; Transgenic animals (Mice, Sheep, Pig, Rabbit, Goat,
Cow and fish). Application of animal cell culture; Bioreactors for animal cell culture;
Mammalian cell products; viral vaccines produced from animal cell cultures.
EXAMINATION:
One question from each unit with internal choice
TEXT BOOKS:
1. Plant Tissue Culture: Theory and Practice: Bhojwani and Razdan (2004).
2. Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney Wiley Liss ,
Wiley, John & Sons, (2005).
3. Animal Cell Culture Practical approach Ed. John, RW Masters. Oxford Publications.
4. Plant propagation by Tissue culture Part I, II and III in practice. EJ George Exegetics Ltd
England
5. Biotechnology by U.Satyanarayana,(2008), Books and Allied(p)Ltd.
6. Plant Tissue Culture Book, Víctor M. Loyola-Vargas, C. De-la-Peña, R. M. Galaz-Ávalos
and F. R. Quiroz-Figueroa, Humana Press Springer, (2008).
REFERENCE BOOKS:
1. Principles of Plant Biotechnology An introduction to Genetic Engineering in Plants2.
3.
4.
5.
6.
7.
8.
Mantal S.H. Mathews J.A., Mickee R.A., Blackwell Scientific Pubs, 1989.
Revolution in Biotechnology- Cambridge University Press, 1989.
Plant Genetic Engineering by John H. Dodds Cambridge University Press, (1985).
Culture of Animal Cells: A Manual of Basic Techniqueby R. Ian Freshney , Wiley-Liss; 5
edition (2005).
Biotechnology in Plant Improvement P.C. Trivedi (ed.) Pub: Pointer Publishers, (2007)
Plant Biotechnology by Michael W. Fowler , Graham S. Warren Pergamon; 1st Edition
(1992)
Plant biotechnology and transgenic plants By Kirsi-Marja Oksman-Caldentey, Wolfgang
Barz, (2006)
Introduction to Plant Biotechnology by H. S. Chawla, Science Publishers; 2nd Edition
(2002).
9. Animal Biotechnology: Comprehensive Biotechnology, by Lorne A Babiuk; John P
Phillips; Murray Moo-Young Publisher: Oxford, New York: Pergamon Press, 1989.
10. Plant Biotechnology A Practical Approach H. S. Chawla, Science Pub. (2003).
11. Cell Separation Fundamentals, Analytical And Preparative Methods Series: Advances In
Biochemical Engineering / Biotechnology , Vol. 106 Kumar, Ashok; Galaev, Igor Yu;
Mattiasson, Bo (Eds.) (2007).
BIOREACTION ENGINEERING
Subject code
: BTT.3.105
Sessional
: 30
Periods / Week : 4
Examination
: 70
Nature of Examination : Theory
Exam Duration
: 3 Hrs
UNIT-1 INTRODUCTION TO REACTION KINETICS
Concepts of Reaction Kinetics, Types of reaction, order of reaction, The effect of temperature
and pH on reaction rate. Rate equations and Reaction mechanisms; Interpretation of batch reactor
data, constant volume batch reactor, integral method of analysis of data for reversible and
irreversible reactions.
UNIT- II REACTION MECHANISM AND GROWTH KINETICS
Searching for mechanism - Arrhenius equation - Batch reactor analysis for kinetics,
(synchronous growth and its application in product production).
Growth Kinetics: Batch growth quantifying cell concentration, growth profiles and kinetics in
batch culture, fed batch growth, continuous growth and their growth kinetic quantification,
chemostat growth, semi-continuous / exponential feeding strategy.
UNIT- III BIOREACTOR SYSTEMS
Definitions, Differences and similarities between chemical and bioreactors; Classification of
bioreactors; Reactor configurations; Description of a conventional bioreactor with all aspects;
Design and construction criteria of a bioreactor; Residence time distributions, concentration, and
temperature distributions; Models of non-ideal reactors.
UNIT- IV DESIGNING OF BIOREACTORS
Design equations for enzyme reactors, batch growth of microorganisms, Design equation of a
plug flow reactor; Design of CSTR with washout concept; Stirred tank reactors with recycle of
biomass; Continuous stirred tank fermentors in series without and with recycle of biomass;
Estimation of kinetic parameters,
UNIT- V MULTIPHASE BIOREACTORS
Different types of reactors: Cell lift reactor, Multi purpose tower reactor, Liquid impelled loop
reactor, Pumped tower loop reactor, Fluidized-bed reactor, Packed bed reactor, Bubble-column
reactors, Airlift reactors.
Animal and plant cell reactor technology- Environmental requirements for animal cell
cultivation, reactors for large-scale production using animal cells, plant cell cultivation.
EXAMINATION:
One question from each unit with internal choice
TEXT BOOKS:
1. Biochemical Engineering by Harvey W. Blanch, Douglas S. Clark CRC; 1 st Edition
(1997).
2. Biochemical Engineering Fundamentals: Solutions Manual by James E. Bailey, David F.
Ollis. McGraw-Hill Education (1979).
REFERENCE BOOKS:
1. Advances in Biochemical Engineering / Biotechnology Series Ed.: Scheper, T.,
2. Biochemical Engineering and Biotechnology Handbook, by Bernard Atkinson Ferda
Mavituna, Grove's Dictionaries; 2nd Edition (1992) .
3. Bioreactor Systems for Tissue Engineering Series: Advances in Biochemical Engineering
/ Biotechnology , Vol. 112 Kasper, Cornelia; van Griensven, Martijn; Pörtner, Ralf ,
(2009), Springer.
4. Biochemical Engineering by Harvey W. Blanch, Douglas S. Clark CRC; 1st Edition
(1997).
5. S.Aiba, A.E. Humphrey and N.R. MHH, "Bio-chemical Engineering", Second Edn.
Academic Press, 1973.
FERMENTATION TECHNOLOGY
Subject code
: BTT.3.106
Sessional
: 30
Periods / Week : 4
Examination
: 70
Nature of Examination : Theory
Exam Duration
: 3 Hrs
UNIT-I INTRODUCTION TO FERMENTATION PROCESSES
The range of fermentation processes; the chronological development of fermentation industry;
Industrial applications; Future trends in fermentations; Aseptic transfer of spore suspension;
Transfer of inoculums from seed tank to Fermentor.
UNIT- II FERMENTATION PROCESSES
General requirements of fermentation processes, Basic design and construction of fermentor and
ancillaries, Main parameters to be monitored and controlled in fermentation processes; An
overview of aerobic and anaerobic fermentation processes and their application in the
biotechnology industry, solid-substrate, slurry fermentation and its applications,
UNIT- III AERATION AND AGITATION IN FERMENTATIONS
Basic Mass transfer concepts; Oxygen transfer from gas bubble to cells; Oxygen transfer in
fermentations; Bubble aeration and Mechanical agitation; Correlations for mass transfer
coefficients; Gas Hold up; Power consumption concepts; Determination of oxygen transfer rates,
KLa values; Other Factors affecting the values of mass transfer coefficients in fermentation
vessels;
UNIT- IV SCALE UP AND RHEOLOGY IN FERMENTATIONS
Scale up of fermentation processes; Principles, theoretical considerations and techniques used;
Scale down methods; The Rheology of fermentation broths; Rheological models; Measurement
of Theological parameters; Rheological Control of fermentations. Mixing concepts, power
requirement for mixing and improvement of mixing in fermentations.
UNIT - V FERMENTORS
Batch, Fed-batch and Continuous Fermentation systems; Dual and multiple fermentations;
Comparison between batch and continuous fermentations; Steady state, unsteady state
continuous fermentation theories; Examples of continuous fermentation; Practical problems with
continuous operations. Monitoring and Control of fermentations, behavior of microbes in
different reactors (air lift, fluidized, batch, and continuous fed batch condition).
EXAMINATION:
One question from each unit with internal choice
TEXT BOOKS:
1. Principles of Fermentation Technology by Peter F. Stanbury , Stephen J. Hall , Allan
Whitaker Butterworth-Heinemann; 2nd Edition (1999)
2. Fundamentals of Biochemical Engineering-Bailey & Ollis Mc. Gr. Hill, 1986
REFERENCE BOOKS:
1. Bioprocess Engineering” by M. Shuler and F. Kargi ((2002)), Prentice Hall of India Pvt.
Ltd., New Delhi.
2. Biochemical Engineering by F C Webb Van Nost. Reinhold (1963)
3. Biochemical Engineering by Harvey W. Blanch), Douglas S. Clark CRC; 1st Edition
(1997).
4. Fermentation Technology (2008) by M.l. Srivastava, Narosa Publishing House, (2008).
5. Practical Fermentation Technology by Brian McNeil , Linda Harvey, Wiley
(2008).
6. “Biotechnology: A Text Book of Industrial Microbiology” 2nd Edition, by W. Crueger &
A. Crueger (2000) Panima Publishing Corporation, New Delhi/Bangalore.
7. Handbook of Food and Beverage Fermentation Technology by Hui , Y.H. Hui , Lisbeth
Meunier-Goddik, Ase Slovejg Hansen , Jytte Josephsen , Wai-Kit Nip , Peggy S.
Stanfield , Fidel Toldra , CRC Press LLC; 1 edition (2004).
8. Essentials of Biochemistry. U.Sathyanarayana. Books and Allied (P) Ltd., Kolkata (1st
Edition), (2002).
BIOPROCESS ENGINEERING LAB- I
Subject code
: BTP.3.107
Sessional
: 25
Periods / Week : 4
Examination
: 50
Nature of Examination : Theory
Exam Duration
: 4 Hrs
LIST OF EXPERIMENTS
1. Preparation of Buffers.
2. Isolation and Extraction of Enzymes
3. Effect of pH on enzyme activity.
4. Effect of Temperature on enzyme activity.
5. Effect of substrate concentration on enzyme activity.
6. Effect of time interval on enzyme activity.
7. Evaluation of enzyme kinetic parameters (Michaeli-Menten approach)
8. Kinetic studies of enzyme inhibition.
9. Deactivation kinetics
10. Enzyme/cell immobilization - matrix entrapment, ionic and cross linking; column
packing;
11. Determination of growth curve of a supplied microorganism and to determine substrate
degradation profile.
12. Development of enzyme assays.
13. Analysis of mass transfer effects on kinetics of immobilized enzyme reactions;
14. Bioconversion studies with immobilized - enzyme packed - bed reactors.
FLUID MECHANICS AND HEAT TRANSFER LAB
Subject code
: BTP.3.108
Sessional
: 25
Periods / Week : 4
Examination
: 50
Nature of Examination : Theory
Exam Duration
: 4 Hrs
LIST OF EXPERIMENTS
1 Determination of discharge coefficient for orifice meter and venturi meter and their
variation with Reynolds number
2
Determination of weir meter constant K for v-notch and rectangular notch
3
Calibration of rotameter and study of variation of flow rate with tube to float diameter
4 Determination of viscosity of Glycerol - water solutions at different temperatures
5 Determination of friction factor for flow of water through annulus using Farmings and
Davos equations.
6 Determination of friction factor for flow through straight pipes of different diameters and
study of variation of friction factor with Reynolds number.
7 Determination of friction losses in pipe fittings
8 Determination of Thermal conductivity of homogeneous wall insulating powder under
steady state conditions.
9 Determination of interface temperatures in composite wall under steady state conditions.
10 Determination of heat transfer coefficient in Natural convection.
11 Determination of over all heat transfer coefficient in unsteady state heat transfer
12 Determination of inside heat transfer coefficient in coil heat exchangers
13 Determination of over all heat transfer coefficient and effectiveness in a Double pipe heat
exchange
14 Determination of heat transfer area in a 1-2- shell and tube heat exchanges
15 Determination of heat transfer coefficient on a single tube by film wise and drop wise
condensation.
FERMENTATION TECHNOLOGY LAB
Subject code
: BTP.3.109
Sessional
: 25
Periods / Week : 4
Examination
: 50
Nature of Examination : Theory
Exam Duration
: 4 Hrs
LIST OF EXPERIMENTS
1.
Culturing of different types of microorganisms (bacteria, yeast, fungi) used in the
production of commercially important products
2.
Preparation of Media
3.
Sterilization of Media and Air
4.
Formulation of simple and complex culture media for fermentations.
5.
Growth of E. coli DH5ά in batch culture.
6.
Fed batch culture techniques
7.
Estimation of growth kinetics.
8.
Study of Growth substrate utilization
9.
Product formation kinetics in shake flask cultures.
10. Bioreactor instrumentation and control.
11. Microbial production of fine chemicals.
12. Estimation of biomass (dry weight); substrate and product analysis;