15
DE–6000
DISTANCE EDUCATION
M.Sc. (Botany) DEGREE EXAMINATION, MAY 2009.
PLANT MOLECULAR BIOLOGY
(2008 onwards)
Time : Three hours
Maximum : 100 marks
Answer any FIVE questions.
Each question carries 20 marks.
1.
2.
3.
4.
5.
6.
7.
(a)
Write short note on chloroplast genome.
(b)
Briefly describe the plant gene structure.
(c)
What are the chloroplast promotor sequences?
(a)
Discuss cytoplasmic male sterility and its causes.
(b)
Briefly describe the expression of nuclear gene.
(c)
Give an account on mitochondrial genome.
(a)
Comment on plant viral vectors.
(b)
Ti plasmid is the best vector for gene transfer in dicot plants – Substantiate.
(c)
Describe the functional organisation of T-DNA.
(a)
Give an account on Binary vectors.
(b)
Write an account on insertion elements.
(c)
Briefly describe the nitrogen fixation in legumes by Rhizobium (nif-genes).
(a)
Comment on Microinjection.
(b)
Enumerate the advantages of agrobacterium-mediated transformation.
(c)
Write note on plant hormones.
(a)
Write short note on Opines.
(b)
Discuss – chloroplast protein genes.
(c)
Briefly describe the mechanism of T-DNA transfer to plants.
(a)
Write short note on molecular pharming.
(b)
Ultrasonification.
(c)
Explain the abiotic plant stress with special reference to salinity.
8.
(a)
What is Bioremediation? Add a note on it.
(b)
What is Gene Tagging?
(c)
Write note on Transgenic Plant derived products for commercial applications.