LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION - BIO-MEDICAL INSTRUMENTATION SCIENCE FIRST SEMESTER – NOVEMBER 2012 BI 1817 - DIAGNOSTIC MICROBIOLOGY Date : 09/11/2012 Time : 1:00 - 4:00 Dept. No. Max. : 100 Marks Section-A Answer ALL of the following: (2x10= 20) 1. Name any two staining techniques to study the characteristics of micro organism. 2. Differentiate primary and secondary barriers of immune system. 3. What are the two categories of infective syndrome? 4. What is Agar? Mention its use. 5. Mention any two nucleic acid – based method for Microbial Identification. 6. Define the term Selective Toxicity. 7. Name any two clinical methods to screen antimicrobial susceptibility screening. 8. Enlist factors affecting the success of vaccine. 9. Name the causative agents of nosocomial infection. 10. Define the term disinfection and give an example of chemical disinfectants. Section-B Answer any FOUR of the following: (4 x 10 = 40) 11. Explain classification of fungus. 12. Discuss the bacterial characteristics used in traditional identification method. 13. Write an account on the mechanism of antimicrobial action with suitable examples. 14. Explain vaccine development strategies. 15. Describe various immunochemical methods for organism detection. 16. Elaborate the good laboratory Practices and the physical design to be adopted. Section-C Answer any TWO of the following: ( 2x 20 = 40) 17. Explain classification of micro-organisms based on nutrition and other growth controlling factors. 18. Discuss the process of disease transmission. 19. Trace the discovery of antibiotic therapy and types of resistance developed by micro-organism. 20. Write in detail the national schedule for immunization adopted in India. Add a note on infection control. *******