The University of Michigan Transgenic Animal Model Core Thomas L. Saunders, Ph.D. 2526 MSRB I, SPC 5674 1150 W. Medical Center Drive Ann Arbor, MI 48109-5674 Voice: (734) 647-2910 FAX: (734) 936-2622 http://www.med.umich.edu/tamc/ Internal Use Only Date Received Log Number Logged By Date of Submission: Service: BAC DNA purification from 250 ml culture (10-25ug). BAC DNA will purified as described: http://www.med.umich.edu/tamc/BACnucleo.html BAC quality will be evaluated by pulsed field gel restriction enzyme mapping. BAC Library and BAC Clone name: Species: Gene of interest: Genome Coordinates and Genome Build: Submit BAC DNA sequence and expected restriction map to Michael Zeidler via email: zeidler@umich.edu Without the sequence we will not be able to verify your BAC. Send a glycerol stock of bacteria containing the BAC clone to 2526 MSRB I on wet ice or 4° C ice packs. Contact Person Phone Number email address: Principle Investigator email address: Authorized Signature