MCB 2004
SAMPLE TEST-1
1.
THAT
UP A
A.
B.
C.
D.
E.
PHASE OF THE GROWTH CURVE AFTER THE BACTERIA HAVE USED
REQUIRED NUTRIENT OR PRODUCED TOO MUCH ACID
LOG PHASE
LAG PHASE
STATIONARY PHASE
MITOTIC PHASE
REPLICATION PHASE
2.
THE DOUBLING TIME OF E. COLI IN NUTRIENT BROTH AT 37o C IS
30 MINUTES. THE INITIAL INOCULUM OF THE CULTURE CONTAINS 25
CELLS. AFTER 2 HOURS OF EXPONENTIAL GROWTH THE CULTURE CONTAINS
A.
100
CELLS
B.
200
CELLS
C.
300
CELLS
D.
400
CELLS
E.
500
CELLS
3.
A BACTERIUM THAT OXIDIZES GLUCOSE WITH OXYGEN FOR ENERGY AND
ALSO USES GLUCOSE AS ITS CARBON SOURCE IS A
A.
CHEMOHETEROTROPH
B.
STRICT ANAEROBE
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
4.
THE FOLLOWING IS ONE POSSIBLE INGREDIENT OF A DEFINED MEDIUM
A.
POTATO EXTRACT
B.
ALANINE (AN AMINO ACID)
C.
BRAIN - HEART INFUSION
D.
YEAST EXTRACT
E.
BEEF INFUSION
5.
PEPTIDOGLYCAN IS MADE OF ______________
BY __________________
A.
PROTEIN
SUGARS
B.
LIPIDS
PEPTIDES
C.
CARBOHYDRATE
SUGARS
D.
CARBOHYDRATE
PEPTIDES
E.
PROTEIN
LIPIDS
6.
HIS DISCOVERY IN 1928 EARNED HIM THE NOBEL PRIZE AND WAS A
BREAKTHROUGH IN ANTIBACTERIAL CHEMOTHERAPY.
A.
JAMES WATSON
B.
ALEXANDER FLEMING
POLYMERS CROSSED-LINKED
C.
D.
E.
VAN LEEUWENHOEK
EDWARD JENNER
LOUIS PASTEUR
7.
DISCOVERIES IN THE TWENTIETH CENTURY IN AREAS OF MICROBIOLOGY
AND MOLECULAR BIOLOGY INCLUDE
A.
THE STRUCTURE OF DNA
B.
THE NATURE OF PRIONS
C
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
8.
THE LIGHT MICROSCOPE
MAGNIFICATION.
A.
0.4 mm,
10
B.
10 m,
100
C.
0.2 m,
2,000
D.
2 nm , 100,000
IS CAPABLE OF ____ RESOLUTION, AND ____
X
X
X
X 
9.
ONE METER EQUALS
A.
100 mm
B.
1,000 m
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
10.
THE PHASE CONTRAST MICROSCOPE IS USEFUL FOR
A.
SEEING BACTERIAL FLAGELLA
B.
VIEWING LIVING BACTERIA
C.
BOTH OF THE ABOVE
D.
NEITHER OF THE ABOVE
KEY FOR QUESTIONS 10-13. THERE IS ONLY ONE CORRECT ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
ESCHERICHIA COLI
BACILLUS SUBTILIS
MYCOBACTERIUM LEPRAE
TREPONEMA PALLIDUM
HOMO SAPIENS (YOU HAD BETTER KNOW THIS ONE)
_____10.
CONTAINS LIPOPOLYSACCHARIDE (LPS)
_____11.
ITS ENVELOPE IS COMPOSED OF TWO LAYERS, A PLASMA
MEMBRANE AND A THICK PEPTIDOGLYCAN CELL WALL
_____12.
IT HAS NO CELL WALL
_____13.
A CLOSE RELATIVE IS NEISSERIA GONORRHOEAE
14.
KILLING ALL ORGANISMS INCLUDING SPORES PRESENT IN A MATERIAL
IS KNOWN AS
A.
FILTRATION
B.
SANITATION
C.
STERILIZATION
D.
LYOPHILIZATION
E.
PASTEURIZATION
15.
ORGANIC COMPOUNDS LIKE ISOPROPANOL (RUBBING ALCOHOL)
PROBABLY HAVE THEIR GREATEST BACTERIAL KILLING EFFECT BY
A.
HYDROLYZING DNA
B.
DESTROYING PROTEINS
C.
DISRUPTING PLASMA MEMBRANES
D.
DISRUPTING SPORES
E.
DISRUPTING RIBOSOMES
16.
__________________ MAKE UP 15% OF THE WET WEIGHT OR
50% OF THE DRY WEIGHT OF CELLS
A.
INORGANIC IONS
B.
CARBOHYDRATES
C.
LIPIDS
D.
PROTEINS
E.
NUCLEIC ACIDS
17.
EUKARYOTIC CELLS ARRESTED IN THEIR GROWTH CYCLE JUST BEFORE
MITOSIS ARE IN
A.
Go
B.
G1
C.
G2
D.
S
E.
M
18.
STREPTOCOCCUS PYOGENES GROWS AS
A.
LONG THIN RODS
B.
CHAINS OF RODS
C.
D.
E.
CLUSTERS OF SPHERICAL CELLS
PAIRS OF SPHERICAL CELLS
CHAINS OF SPHERICAL CELLS
19.
KOCH'S POSTULATES DO NOT INCLUDE
A.
THE PATHOGEN MUST BE PRESENT IN EVERY CASE OF DISEASE
B.
THE PATHOGEN MUST BE ISOLATED & GROWN IN PURE CULTURE
C.
THE PATHOGEN MUST BE TRANSMITTED TO ANOTHER HOST BY
AEROSOLIZED DROPLETS
D.
THE PATHOGEN IS REISOLATED FROM THE INOCULATED HOST &
SHOWN TO BE IDENTICAL TO THE ORIGINAL ORGANISM
20.
THIS CARBOHYDRATE POLYMER IS FOUND IN THE ENVELOPE OF GRAM
NEGATIVE CELLS
A.
CELLULOSE
B.
TEICHOIC ACID
C.
GLYCOGEN
D.
LIPOPOLYSACCHARIDE
E.
CHITIN
KEY FOR QUESTIONS 22-25. EACH QUESTION HAS ONLY ONE ANSWER, BUT EACH
ANSWER MAY BE USED MORE THAN ONCE (OR NOT AT ALL).
A.
B.
C.
D.
E.
THE TCA CYCLE
PROTEIN SYNTHESIS
LACTIC ACID FERMENTATION
THE ELECTRON TRANSPORT CHAIN
GLYCOLYSIS
_____22.
IN THE FIRST STEP, OXALOACETIC ACID ACCEPTS ACETATE
FROM ACETYL-COA TO PRODUCE CITRIC ACID
_____23.
ANABOLISM OF A BIOPOLYMER
_____24.
IN MUSCLE CELLS IN THE ABSENCE OF OXYGEN, GLUCOSE IS
METABOLIZED BY THIS OVERALL PROCESS
_____25.
GLUCOSE IS CONVERTED TO 2 PYRUVIC ACIDS
*************************************************
SHORT ANSWER QUESTIONS (10 POINTS)
26.
THERE ARE TWO GENERAL METHODS FOR COUNTING BACTERIAL CELLS BIOLOGICAL (GROWTH) METHODS AND PHYSICAL METHODS. DESCRIBE ONE
PROCEDURE
FOR EACH TYPE.
OR
27.
DRAW A DIAGRAM OF A GRAM POSITIVE CELL ENVELOPE AND LABEL IT.
SHOW THE GENERAL LOCATION OF THE PHOSPHOLIPID, THE PROTEIN
AND THE TEICHOIC ACIDS.
--------------------------