Mold Terrarium Summary: Grade Level: 4-8 After a short introductory game explaining the role of fungi as decomposers, students will create their own “mold terrariums” (using common food items, water, and yeast). They will let these “grow” in a dark place for a week, and return to see the results! Objectives/ Standards: The students will… 1) Participate in a lesson/ activity about the role of fungi in the environment. 2) Design experiments to test the growth of fungi on common food items. 3) Examine the results of their experiments to learn more about fungi. Background: A nutrient is a substance required for life, but which cannot be created by the body, and so must be taken in from the environment. When a living animal or plant dies, its nutrients remain trapped within the body, but not forever. If nutrients became trapped forever when an animal died, our world would quickly become nutrient poor, and inhospitable further life. Thankfully, nutrients are constantly cycled through the environment, from dead matter back into living matter, by fungi, bacteria, and other small critters that help decompose dead organic matter and reintroduce the nutrients into the food chain. Fungi are everywhere, particularly where organic matter is plentiful. There are about a million fungi in one ounce of rich topsoil. Fungi include mushrooms, brackets, puffballs, truffles, molds, and yeast. The mushrooms and toadstools we usually see are the fruiting, or reproductive, structures of the fungi (like the flowers on plants). But unlike plants, fungi don’t produce seeds. Instead, they reproduce from spores contained in the fruiting bodies. These spores float through the air and land on stuff. When one lands on a damp piece of food, the fungus grows as it consumes the organic matter of its host. The fruiting bodies we typically see are only a very small part of a much larger non-reproductive network of fungal filaments (mycelium) found within the soil or decaying matter. Unlike plants, fungi do not contain chlorophyll and cannot change sunlight into carbohydrates (food), so they must feed on other organisms. It is through the mycelium that a fungus absorbs nutrients from its environment, such as dead plant and animal matter. The mycelium of “mycorrhizal” fungi can form a mutually beneficial relationship (mutualism) with surrounding plants, which increases the efficiency of water and nutrient absorption to plants, and even confers resistance to some plant pathogens! How? The fungi attach to plant roots underground to gain access to carbohydrates which it cannot produce on its own, and this in turn gives the plant access to the increased absorptive powers of the mycelim, especially phosphates. Essential Questions: What role do fungi play in sustaining life on earth? How are fungi different from plants? How are nutrients constantly cycled through the environment? Duration: 40 min Indoor/ Outdoor/ Both: Key terms: Fungi mycelium decomposers mold yeast spores nutrients cycle food chain mutualism Materials: o Variety of food items (breads, cheese, fruit, with preservatives and without) o Clear containers (plastic baggies or liter bottles cut open work fine) o Yeast (optional) o Tape o Water o Permanent markers o Newspaper o o Notes: Do not use meat or fish, as they will become very smelly! Set-up: a. Have table space prepared for students to work with food and water. b. Have a space prepared for students to play a semi-active game involving moving around and making silly noises! Lead-In: Introduce the Nutrient Cycling Game where nutrients are passed from plants animals fungi plants, by means of winning games of rock-paper-scissors (RPS). RULES: Students play the role of nutrients which are being constantly cycled through the environment. Students mill around the playing area (classroom) playing RPS with each other in order to change status from (be taken up by) plant to animal to fungus. Each student starts as nutrients in a plant (ask them to think of their favorite plant and act like it), and must win a game of RPS in order to be taken up by an animal (ask them to think of their favorite animal and act like it). Students must again win RPS to advance to be taken up by fungi (act like a mushroom?). Students may only play RPS with someone of an equal status (fungi vs. fungi, etc), and the loser stays at the same level. When students advance beyond fungi status they again become plants, because many fungi pass nutrients to plants. Notice there is no top status – because this game is meant to demonstrate a cycle! When everyone has gone through the cycle several times, end the game. Have students return to their seats. The Activity: 1. 2. 3. 4. 5. 6. Explain that you are going to be making terrariums to grow fungi. What will they need to survive? Explain how fungi are very different from plants (see Background). Assign students into groups of 2-4. Seat groups around a table and supply newspaper, water, and clear containers. Allow students to choose 1 food item to place in their container. Have them lightly moisten dry food items before putting them into a container. Optionally, have students add a PINCH of yeast to their container. Have them seal their containers well and write a namezz and date on it. Store in a dark place until next week… *If time allows, you may want to encourage groups to experiment with 2 different variables. Ex: Groups could create 2 containers that are identical, except: 1 has water, 1 does not, OR 1 is kept in the dark, 1 in the light, OR 1 has yeast added, 1 does not, OR 1 with air, 1 with air removed, etc… Wrap-up (how will you help students “bring it home”?): a. One week later, have students compare and contrast their containers. a. What color/ texture is the mold? b. What foods got moldy quickest? c. Did anything not get moldy? d. Do different types of molds grow on different types of foods? e. Which decomposed the most/ least? f. Which showed the greatest growth of fungi (mold)? g. Were their predictions right or wrong? h. How can you keep your food fresh? i. Do fungi like to eat preservatives? b. Do not open the containers, just throw them away. Mold is not healthy to inhale. Extensions (Where can students/ teachers go from here? What lessons could come next?): A. Bacteria at Work – a lesson comparing rates of bacterial growth on potato wedges buried under soil. B. Compare rates of plant growth with and without fungal relationships. (Mycorrizal spores can be obtained online or at garden centers). C. Relevant Books: Other Relevant Activities/ Games: Notes: