Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Student Author Name: A Somebody
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Team Members Name(s):
Tardy
Barbie
Ana Noyance
Yucan Nottatuchiths
Student Author ID #: 10123456
Team Members ID #’(s):
1458971
1085546
236548
Lab Assignment #1
Team Name:
Lab Assignment Title: Human Gametogenesis
Date: 20150903
Purpose / Objective(s):
1.
2.
3.
4.
Observe Cheek Cells
Observe Human Gametogenesis in prepared MS Slides via standard Light Microscopy
Survey and capture illustrations and photos from the internet of the above
Measure Cell and Structure Size utilizing Field of View Calculator
Hypothesis:
NA
Materials / Subjects / Specimens:
Specimens
1. Cheek cells from self
2. Prepared MS slides:
1. Testis
2. Sperm
3. Ovary
4. Oocyte
5. Maturing Follicle
6. Onion Root
7. Whitefish Blastula
Materials

Methylene Blue Stain

Toothpicks

MS Slides and Coverslips
.
Page 1 of 9
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Methods / Tools / Instrumentation / Procedures:
Instruments

Standard Binocular Light Microscope

Brand and model Number…see Photo 1 in Attachments
Procedures
1. Prepare Human Cheek cell MS slide using standardized methylene blue stain
2. View Human Cheek cell all magnifications
3. Prepare Human hair MS Slide
4. Viewed Human hair at all magnifications
5. View Human sperm smear, and view ovary at all magnifications
6. Viewed Letter e, Colored Threads as MS practice specimens
7. Viewed Mitosis in Onion Root and White fish Blastula
Measurements and Calculations

Used the Cell Size FOV On Line Calculator to estimate the sizes of (diameters) of all specimens
See Figure 1 in Attachments
Results

Viewing the target specimens and structures with the standard light microscopes required skill and
patience.

Making photographs of the specimens using my Cell Phone Camera required skill beyond my ability.




Gametogenesis tissues and cells are presented in Table 1
o Male and Female structures
Sample measurements from the FOV Calculator are presented in Table 2
Table 3 lists the size measurements for the cells and structures of Gametogenesis and Mitosis
Figure 1 compares the cells and structure sizes of Gametogenesis and Mitosis Specimens
Analysis / Discussion:

Compare and Contrast

…

….

….

…..
Page 2 of 9
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Research
Phases of Mitosis
http://www.biology.iupui.edu/biocourses/N100/images/8mitosiscropped.jpg
Phases of Meiosis
Page 3 of 9
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Conclusions/Further Considerations: Take Away



The Microscope is a valuable essential device for the study of cells and histology.
………..
………….
References
1. http://webnt.calhoun.edu/distance/internet/natural/anatomy/EndoRepro/ovary4X.html
2. http://faculty.baruch.cuny.edu/jwahlert/bio1003/mitosis.html
Page 4 of 9
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
ATTACHMENTS:
Table 1:
Illustrations and Photomicrographs of cells- Human Gametogenesis, (Testis, Sperm, and Ovary) and
Human Development
Table 1
Human
Gametogenesis
Specimen
Key Events
Testis
Spermatogenesis Meiosis
Web Reference
Notes / Description /Illustration
Low Magnification
Photo
Medium Magnification
Photo
High Magnification
Photo
http://www.bing.com/images/search?q=Testis+M odel&vie
w=detailv2&&id=FAD0D6375C7F1CBDF8FEC27A94A3F
4A91CACA823&selectedIndex=40&ccid=uFxvgOai&simid
http://www.bing.com/images/search?q=Testis+M odel&vie
w=detailv2&id=6AABF80730719E94F749F699174AEAE
B6C746A45&selectedindex=38&ccid=%2BTEhpdiO&simid
http://www.bing.com/images/search?q=human+testis+med
ium+magnification+histology&view=detailv2&&id=346B81
0E391D82012C85F162172A3994BEA93759&selectedInd
http://www.bing.com/images/search?q=human+testis+med
ium+magnification+histology&view=detailv2&id=DA3C78B
78196F1733D03E3FB7A7C374046CC0A09&selectedind
Very High Magnification
Photo
Cell Size
Sperm
Maturation Discharge into Lumen of
Seminiferous Tubuls
Web Reference
http://bigthink.com/ideafeed/how-sequencing-spermcould-help-fight-cancer
Cell Size
Ovary
Oogenesis by Meiosis
Web Reference
Cell Size
Oocytes
Mature from Primary to Secondary
Web Reference
http://www.firstscience.com/home/images/stories/egg.jpg
Cell Size
Mature Follicle
Web Reference
Cell Size
Page 5 of 9
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Table 2: Specimens of Gametogenesis and estimated sizes in mm and microns
Average Size mm
Average Size μm
Human Sperm
0.00
2.8
Onion Root Cell
0.01
14.6
Blastula Cell
0.02
15.2
My Hair
0.02
22.9
Cheek Cell
0.06
55.9
Colored Threads
0.08
76.1
Human Oocyte
0.15
145.8
White Fish Blastula
0.70
700.0
Letter e
1.05
1053.5
Corpus Luteum
3.50
3500.0
Specimen
Page 6 of 9
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Figure 1 Estimated cell Size in mm
Average Size mm
3.50
3.00
2.50
Human Sperm
Onion Root Cell
Blastula Cell
2.00
My Hair
Cheek Cell
Colored Threads
1.50
Human Oocyte
White Fish Blastula
Letter e
1.00
Corpus Luteum
0.50
0.00
Human
Sperm
Onion Root
Cell
Blastula
Cell
My Hair
Cheek Cell
Colored
Threads
Human
Oocyte
White Fish
Blastula
Letter e
Corpus
Luteum
Page 7 of 9
612952380
Biology 105 – Human Biology
Session 2015:
Sections:
Class Location:
Days / Time :
Lab Report
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Illustration 1
Example of Cell Size Calculator
FOV Field of View
Check / Set
Specimen Size
Therefore
OCULAR
MAG
Therefore
The Field of
View
Diameter is
Magnification
Name your
specimens here
Therefore
OBJECTIVE
MAG
TOTAL
MAG
1,000
Specimen
Estimated Count *
Pi times radius
Put your estimate here
squared
-
How many specimens
(S) could be placed
"shoulder to shoulder?"
in a straight line along
the diameter of the
FOV?
For a
Specimen with
a Round Form
Ratio
The Specimen
Diameter
=
FOV Area in μm
squared
FOV /
Specimen
units in
mm
1,000,000
1
The Field of
View Area is
FOV
FOV Area in
FOV Diameter
Diameter in
mm
in μm
mm
squared
Size for Round and Spherical
Set Ocular and Objective
Powers here
Count =
units in microns
1,000
mm
μm
Cheek Cell
10
4
40
3.50
3500
9.62
9,621,150
59.0
0.017
0.06
59
Cheek Cell
10
10
100
1.40
1400
1.54
1,539,384
24.0
0.042
0.06
58
Cheek Cell
10
40
400
0.35
350
0.10
96,212
7.0
0.143
0.05
50
10
63
630
0.22
222
0.04
38,785
292.5
1.4
1368.1
2.8
Cheek Cell
AVERAGE
Specimen
2,823,883
Set Ocular and Objective
Powers here
30.0
0.1
Count =
0.056
55.9
mm
μm
Colored Threads
10
4
40
3.50
3500
9.62
9,621,150
35.0
0.029
0.10
100
Colored Threads
10
10
100
1.40
1400
1.54
1,539,384
20.0
0.050
0.07
70
Colored Threads
10
40
400
0.35
350
0.10
96,212
6.0
0.167
0.06
58
Colored Threads
10
63
630
0.22
222
0.04
38,785
292.5
1.4
1368.1
2.8
AVERAGE
2,823,883
20.3
0.1
0.1
Page 8 of 9
76.1
612952380
Biology 105 – Human Biology
Lab Report
Session 2015:
Sections:
Class Location:
Days / Time :
Instructor:
Fall
60544 4 Units
Fall
64561 4 Units
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
T 9:30 AM – 12:20 PM LAB
RIDDELL
838 / 2030
T Th 8:00 AM – 9:20 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Photo 1 Microscope
Jkosdfnglnvlfvdflpvpsdfkvpfv
Page 9 of 9
612952380