ENVIRONMENTAL RISK MANAGEMENT AUTHORITY DECISION Amended under s67A on 1 October 2006 21 December 2000 Application codes Application type Consideration period Considered by GMC99021 Refer to Appendix 1 for applicant details. Import into Containment any New Organism under section 40(1)(a) of the Hazardous Substances and New Organisms (HSNO) Act 1996, made in accordance with section 259 for the approval of existing new microorganisms. 18 – 30 October 2000 Special Committee of the Authority appointed under section 19(2)(b) of the HSNO Act 1996. Decision The application is Approved with Controls. The organisms approved for the application are the microorganisms listed in Appendix 2 to this decision. Application Process Refer to Appendix 1 for details of the application processes. The application was not required under the Act to be publicly notified. The documents available for the evaluation and review of the application by ERMA New Zealand included the application and information from discussions held with the Department of Conservation and the Ministry of Agriculture and Forestry. As the group of applications originally considered involved both genetically modified and non-modified organisms a special committee was established by the Authority to combine members from both the GMO and Non-GMO Standing Committees. The Special Committee of the Authority was appointed in accordance with section 19(2)(b) of the Hazardous Substances and New Organisms (HSNO) Act 1996. The committee comprised members of the Authority: Mrs Helen Hughes (Chair), Dr Lindie Nelson and Professor Barry Scott. Relevant Legislative Criteria The applications sought approval in accordance with section 259 of the HSNO Act 1996 to maintain in containment, and to re-import into containment, microorganisms held as reference specimens for reference and national and international research programmes. These organisms were lawfully imported into New Zealand before July 1998, and the application was made under Part V of the HSNO Act 1996, to bring previously lawful practice into line with HSNO requirements. Section 259 applies to existing microorganisms, where no permit was held that could be deemed to be an approval under section 241(1) or (3) of the Transitional Provisions (Part XVI) of the HSNO Act. The applications were lodged pursuant to section 40(1)(a) of the HSNO Act. The decisions were determined in accordance with section 45, after taking into account the additional matters under sections 37 and 44 of the HSNO Act, and those relevant matters under Part II of the HSNO Act. Consideration of the applications followed the relevant provisions of the Hazardous Substances and New Organisms (Methodology) Order 1998, but with particular regard to clauses 8 (dealing with the scale and significance of the risks, costs and benefits) and 26 (dealing with applications where the risks are negligible). Reasons for the Decision Purpose The Committee considered that the purpose of the applications (refer to Appendix 1) was to gain approval for existing collections of microorganisms for reference, and research programmes. In accordance with section 45(1)(a)(i) of the Act, the Committee determined that this was an appropriate purpose under section 39(1)(h). Ability to Escape Containment In accordance with section 44(b) the Committee considered the ability of the organisms to escape from containment. Currently the microorganisms are held in containment at the applicants’ research facilities throughout New Zealand. The locations are either approved under MAF’s Transitional Facilities Standard and MAF/ERMA New Zealand Standard 154.03.02: Containment Facilities for Micro-organisms, or are in the process of gaining approval under Standard 154.03.02. The facilities will all require approval at physical containment level 1 or 2, depending on the organisms in the collections. All the containment facilities require approval by MAF under the MAF/ERMA Standard 154.03.02: Containment Facilities for Microorganisms. D:\401286708.doc Page 2 of 11 In some facilities, for logistical simplicity, all the microorganisms (of both Risk Group 1 and 21 as defined in the Australian/New Zealand Standard 2243.3:20024, Safety in Laboratories: Part 3: (Microbiology) are held at the higher level PC2 containment. The Committee notes that these collections have been maintained for many years without reported instances of unintended or accidental release from containment. The collections are of pure cultures, which are used in laboratories, and are handled by researchers with experience in microbiology. The Authority understands that most of the collections are held ex situ on artificial media or under extremely low temperatures (e.g. minus 80°C) and cannot simply escape or be accidentally released. The Committee concludes that, taking into account the laboratory procedures proposed by the applicants and the containment controls imposed in this decision, it is very unlikely that the microorganisms could escape from the containment facility. Establishment of self-sustaining populations and ease of eradication In accordance with sections 37(a) and (b) of the HSNO Act the Committee considered the ability of the organisms to establish undesirable self-sustaining populations, which might cause adverse effects and the ease with which any such populations could be eradicated in the event of any escape from containment. Many of the microorganisms and GMOs are laboratory strains, that require specialised culture regimes and/or growth factors to survive, and so have a low likelihood of survival outside of the laboratory. Some of the organisms held in the collections may already occur within the environment, so that any escape from containment is unlikely to have a significant effect. Some of the microorganisms do not have their hosts in New Zealand, or their hosts are either locally distributed or uncommon so that in the event of an escape, access of propagules to the host and therefore establishment will both be unlikely. Many other variables, including climate and the life stage of the organism will also determine the ability for an escaped pathogen to establish an infection. If microorganisms did escape and establish self-sustaining populations it is likely that only disease causing pathogenic organisms would be detected because of their adverse effects on host plants or animals. In this situation there would be potential for control of the pathogens with pesticides. However, in the event of soil microorganisms establishing self-sustaining populations it is unlikely that they would be detected and therefore little opportunity is available to control the spread or attempted eradication. Also any control mechanism for escaped soil organisms, which are detected, could have adverse impacts on the natural soil microbiota. 1 Risk group 1: (low individual and community risk) Microorganisms that are unlikely to cause human, plant or animal disease (as defined in the Australia New Zealand Standard - Safety in laboratories Part 3: Microbiology 2243:3 1995). Risk Group 2: (moderate individual risk, limited community risk) A pathogen that can cause human, plant or animal disease, but is unlikely to be a serious hazard to laboratory workers, the community, livestock, or the environment; laboratory exposures may cause infection, but effective treatment and preventative measures are available, and the risk of spread is limited (as defined in the Australia New Zealand Standard - Safety in laboratories Part 3: Microbiology 2243:3 1995). D:\401286708.doc Page 3 of 11 The Committee concludes that in the unlikely event of any self-sustaining population of microorganisms, particularly soil microorganisms becoming established, the organisms would be difficult to eradicate, in most cases. The Committee notes that the applicants are required, as part of the facility registration to maintain an internal Containment Manual, which must include contingency plans detailing the procedures to be implemented to deal with the spillage of any microorganisms within and outside the facility and for fire or other emergencies that may compromise containment. Potential adverse effects The Committee considered the potential adverse effects of the microorganisms on the environment and public health. Any effects can only be realised should the organisms escape, survive and establish self sustaining populations, or should laboratory staff come in contact with harmful microorganisms. Environment In identifying and assessing the risks associated with these applications the applicants have relied on the fact that these organisms are classified as Risk Group 1 and/or Risk Group 2 organisms, and therefore are unlikely to cause human, plant or animal disease (Risk Group 1) or are unlikely to be serious hazards to laboratory workers, the community, livestock or the environment (Risk Group 2). Many of the organisms in these collections have been held in containment for many years and, to the best of the applicants’ knowledge, have not had an adverse effect on the environment or human health. Some of the microorganisms may be able to infect native or exotic flora or fauna, and some may occur on genera that are found in New Zealand. Consequently, in the event of an escape from containment there is the potential for the microorganisms to establish and have an adverse effect on native or valued exotic flora. Public health The Committee notes that many of the organisms in these collections have already been held in containment for many years and, to the best of the applicants’ knowledge, have not had adverse effects on human health. Good microbiological practice will minimise the potential for any human pathogens included in this approval to infect laboratory workers. Ethical Considerations The Committee acknowledges that some of the genetically modified organisms held in these collections contain genetic material sourced from humans. As far as could be ascertained no human genetic material is sourced from Māori. The applications are not for the development of GMOs but for the maintenance of existing collections of GMOs developed either internationally or in New Zealand. The Committee considered that any ethical approvals required for the future use and further development or re-importation of the GMOs would be covered by the importing institutions’ ethical guidelines and Institutional Biological Safety Committee (IBSC) delegation requirements. D:\401286708.doc Page 4 of 11 Inseparable Organisms In accordance with section 45(1)(a)(ii) the Committee considered the effects of any inseparable organisms. As noted above the applicants explained that all the collections are of pure cultures. Some applicants have noted that the possibility of inseparable organisms being present in imported cultures could not be excluded. The Committee notes that since the organisms are imported or held as “pure” cultures, contaminating organisms would be readily noticed in many cases. The Committee considers that, in this application, any inseparable organisms are very unlikely to pose risks, given that the organisms are very unlikely to escape containment under the specified containment controls. Negligible Risk Based on the consideration and analysis of adverse effects to the environment and public health, and taking into account the requirements for containment and that specialist research staff handle the organisms, the Committee considers that the risks associated with the maintenance and importation into containment of the microorganisms listed in Appendix 2 are negligible. Benefits The Committee has identified the primary benefit as being the increase in scientific knowledge as through using the microorganisms for national and international research and for reference purposes. The Committee is satisfied that costs are unlikely to accrue to parties other than the applicants. Conclusion The microorganisms are required to be contained in facilities that are registered, operated and managed in accordance with MAF/ERMA New Zealand Standard 154.03.02: Containment Facilities for Microorganisms, at physical containment level PC1 for Risk Group 1 organisms or PC2 for Risk Group 2 organisms, and the additional controls imposed in this decision. Having considered the possible effects of the organism in accordance with sections 45(1)(a)(ii) and (iii) of the HSNO Act, the Committee is satisfied that the proposed containment regime and additional controls can adequately contain the microorganisms. The Committee concludes that the risks arising from maintaining and importing the microorganisms into containment for reference and research purposes are negligible. Therefore, the Committee has considered this application in terms of clause 26 of the Methodology. Taking into account the containment controls, the Committee considers that the beneficial effects of having the organisms in containment outweigh the likely adverse effects of the organisms and any inseparable organisms, should they escape. In this case, the fact that the applications have been made indicates that the benefits to the applicant outweigh the costs. The Committee is satisfied that costs are unlikely to accrue to parties other than the applicant. D:\401286708.doc Page 5 of 11 The application is approved subject the following controls in accordance with sections 45(1)(a) and 45(2) of the HSNO Act. D:\401286708.doc Page 6 of 11 Controls 1. To limit the likelihood of any accidental release of any organism or any viable genetic material2: 1.1 The construction and operation of the various containment facilities (“the facility”) in which the genetically modified and non-genetically modified microorganisms (as listed in Appendix 2) are maintained and re-imported into shall be in accordance with the: a) Ministry of Agriculture and Forestry (MAF) Biosecurity Authority/ERMA New Zealand Standard 154.03.023: Containment Facilities for Microorganisms. b) Australian/New Zealand Standard AS/NZS 2243.3:20024 Safety in Laboratories: Part 3: (Microbiological aspects and containment facilities), at Physical Containment Level 1 (PC1) for organisms in Risk Group 15. c) Australian/New Zealand Standard AS/NZS 2243.3:20024 Safety in Laboratories: Part 3: (Microbiological aspects and containment facilities), at Physical Containment Level 2 (PC2) for organisms in Risk Group 25. 1.2 The containment facilities shall be approved by MAF in accordance with the MAF Biosecurity Authority/ERMA New Zealand Standard 154.03.023, and the controls of the Authority. 1.3 The minimum requirement for packaging for transportation of the microorganisms by all modes (ie. air, land and sea) from overseas and for transfers between facilities the organism shall be according to Packaging Instruction No. 650 of the International Air Transport Association (IATA) Dangerous Goods Regulations (refer to MAF/ERMA New Zealand Standard 154.03.023). All containers must be clearly labelled with the name, address and phone number of both the sender and the recipient. 1.4 All practical and feasible measures shall be taken to ensure viable fungal material, including spores, are contained and do not leave the containment facility through any outlets including exhaust system, air filters and water discharge. This control may be met by ensuring that all work with any sporulating or potentially sporulating fungi occurs in a biological safety cabinet of Class II, in which any water used is sterilised before disposal. Other methods may also be available to achieve this control. Viable Genetic Material is biological material that can be resuscitated to grow into tissues or organisms. It can be defined to mean biological material capable of growth even though resuscitation procedures may be required, eg when organisms or parts thereof are sublethally damaged by being frozen, dried, heated, or affected by chemical. 2 3 Or any updated Standard approved by ERMA New Zealand 4 Or any updated version endorsed by ERMA New Zealand 5 As identified in organism descriptions of Appendix 2 to this decision D:\401286708.doc Page 7 of 11 2. To exclude unauthorised people from the facility 2.1 Construction and operation of the containment facility shall comply with the requirements contained in the standards listed in control 1.1 relating to identification of entrances, numbers of and access to entrances, and security requirements for the entrances and the facility. 3. To exclude other organisms from the facility and to control undesirable and unwanted organisms within the facility 3.1 Construction and operation of the containment facility shall comply with the standards listed in control 1.1 relating to exclusion of other organisms from the facility and the control of undesirable and unwanted organisms within the facility. 4. To prevent unintended release of the organism by experimenters working with the organism 4.1 Construction and operation of the containment facility shall comply with the requirements of the standards listed in control 1.1 relating to the prevention of unintended release of the organism by experimenters working with the organism. 5. To control the effects of any accidental release or escape of an organism 5.1 Construction and operation of the containment facility shall comply with the requirements contained in the standards listed in control 1.1 relating to controlling the effects of any accidental release or escape of an organism. 5.2 If a breach of containment occurs the Facility Operator must ensure that the MAF Inspector6 responsible for supervision of the facility has received notification of the breach within 24 hours. 5.3 In the event of any breach of containment the contingency plan for the attempted retrieval or destruction of any viable material of the organisms that have escaped shall be implemented immediately. The contingency plan shall be included in the containment manual in accordance with MAF/ERMA New Zealand Standard 154.03.023: Containment Facilities for Microorganisms. 6. Inspection and monitoring requirements for containment facilities 6.1 The operation of the containment facility shall comply with the requirements contained in the standards listed in control 1.1 relating to the inspection and monitoring requirements for containment facilities. 6.2 The Authority, or its authorised agent or properly authorised enforcement officers, may inspect the facilities at any reasonable time. 6.3 The containment manuals shall be updated, as necessary, to address the implementation of the controls imposed by this approval, in accordance with MAF/ERMA New Zealand Standard 154.03.023: Containment Facilities for Microorganisms. 6 An inspector appointed under the Biosecurity Act D:\401286708.doc Page 8 of 11 7. Qualifications required of the persons responsible for implementing those controls 7.1 The operation of the containment facility shall comply with the requirements contained in the standards listed in control 1.1 relating to the training of personnel working in the facility. 7.2 The person in charge of a particular research area and/or the person in charge of the operation of the containment facility shall inform all personnel involved in the handling of the organisms of the Authority’s controls. ___________________ Date: 21 December 2000 Mrs Helen Hughes, Chair Special Committee of the Authority Amendments: August 2006; to split the 19 applications from one decision to enable section 67A amendments to be made to individual decisions update of the containment facility references in the controls to the most recent MAF/ERMA technical standards and correct the Australian/New Zealand containment facility references to “future proof” the decisions Dr Kieran Elborough Acting Chair, New Organisms Standing Committee Date: 1 October 2006 D:\401286708.doc Page 9 of 11 Decision Appendix 1 – Application details 1. Application Code Applicant Purpose Date Received Date Verified GMC99021 Genesis Research and Development Corporation Limited To gain approval in accordance with section 259 of the HSNO Act 1996 to maintain in containment microorganisms which are genetically modified laboratory strains for use as routine tools. First received 28/09/1999 Resubmitted 10/02/2000 Resubmitted 06/03/2000 09/03/2000 Contacts: Dr Lenny Bloksberg Dr Elizabeth Visser D:\401286708.doc Page 10 of 11 Decision Appendix 2 –List of Approved Organisms D:\401286708.doc Page 11 of 11 Application: GMC99021 Approval Code GMC000374 Application Code GMC99021 GMC000375 GMC99021 A. tumefaciens LBA4301wt A. tumefaciens GMC000376 GMC99021 A. tumefaciens. A. tumefaciens LBA4301ros1/pTiB6S3SE/p MON200 (RC) GMC000377 GMC99021 A. tumefaciens. A. tumefaciens GV3111/pTiB6S3SE/pMON 200 (RC) GMC000378 GMC99021 A. tumefaciens. LBA4011ros1/pJK270 A. tumefaciens GMC000379 GMC99021 A. tumefaciens. LBA4011wt/pJK270 A. tumefaciens GMC000380 GMC99021 A. tumefaciens. LBA4301/ros1pJK270 A. tumefaciens GMC000381 GMC99021 A. tumefaciens. LBA4301wt/pJK270 A. tumefaciens GMC000382 GMC99021 A. tumefaciens. A. tumefaciens LBA4301wt/pTiB6S3SE/pM ON200 (RC) D:\401286708.doc Common Name A. tumefaciens LBA4301ros1 Taxonomic Name A. tumefaciens Organism Description Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301ros1 Plasmid(s): Description: Rec minus Agro host with with ros mutation and no Ti-Plasmid. FMB: 6 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): Description: Rec minus Agro host with no Ti-Plasmid. FMB: 5 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301ros1 Plasmid(s): pTiB6S3SE/pMON200 (RC) Description: Transformation Control with Kan resistance. FMB: 8 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: GV3111 Plasmid(s): pTiB6S3SE/pMON200 (RC) Description: Transformation Control with Kan resistance. FMB: 1 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4011ros1 Plasmid(s): pJK270 Description: pTiC58 containing Tn5, a Wild type pTiC58 with Km resistance FMB: 30 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4011wt Plasmid(s): pJK270 Description: pTiC58 containing Tn5, a Wild type pTiC58 with Km resistance FMB: 29 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301ros1 Plasmid(s): pJK270 Description: pTiC58 containing Tn5, a Wild type pTiC58 with Km resistance FMB: 28 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pJK270 Description: pTiC58 containing Tn5, a Wild type pTiC58 with Km resistance FMB: 27 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pTiB6S3SE/pMON200 (RC) Description: Transformation Control with Kan resistance. FMB: 7 Approval Code GMC000383 Application Code GMC99021 GMC000384 Common Name Taxonomic Name A. tumefaciens. pAL4404 A. tumefaciens GMC99021 A. tumefaciens. pAL4404, pB129 A. tumefaciens GMC000385 GMC99021 A. tumefaciens. pAL4404, pB130 A. tumefaciens GMC000386 GMC99021 A. tumefaciens. pAL4404, pBKM17 A. tumefaciens GMC000387 GMC99021 A. tumefaciens. pAL4404, pBKM18 A. tumefaciens GMC000388 GMC99021 A. tumefaciens. pAL4404, pBKM19 A. tumefaciens GMC000389 GMC99021 A. tumefaciens. pAL4404, pBKM25 A. tumefaciens GMC000390 GMC99021 A. tumefaciens. pAL4404, pBKM26 A. tumefaciens GMC000391 GMC99021 A. tumefaciens. pAL4404, pBKM27 A. tumefaciens GMC000392 GMC99021 A. tumefaciens. pAL4404, pBKM30 A. tumefaciens D:\401286708.doc Organism Description Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404 Description: VIR plasmid. FMB: 33 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA44404 Plasmid(s): pAL4404, pB129 Description: Binary T-DNA vector with NPTII FMB: 41 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pB130 Description: Binary T-DNA vector with NPTII and ONC FMB: 42 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM17 Description: pCIBD10g containing AthCOR15a, a T-DNA / 180bp PmlI-PmlI>CAMV-90>GUS FMB: 265 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM18 Description: pCIBD10g containing AthCOR15a, a T-DNA / 498bp EcoRI-ClaI>CAMV-90>GUS FMB: 262 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM19 Description: pCIBD10g containing CAMV-90, a T-DNA / CAMV90>GUS control FMB: 269 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM25 Description: pCIBD10g containing AthCOR15a, a T-DNA / C-Box 7mer MT50/51>CAMV-90>GUS FMB: 268 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM26 Description: pCIBD10g containing AthCOR15a, a T-DNA / 27bp C-Box MT50/51>CAMV-90>GUS FMB: 267 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM27 Description: pCIBD10g containing AthCOR15a, a T-DNA / mutCBox PmlI-PmlI>CAMV-90>GUS FMB: 266 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Approval Code Application Code Common Name Taxonomic Name GMC000393 GMC99021 A. tumefaciens. pAL4404, pBKM31 A. tumefaciens GMC000394 GMC99021 A. tumefaciens. pAL4404, pGLAM1 A. tumefaciens GMC000395 GMC99021 A. tumefaciens. pAL4404, pLCT71 A. tumefaciens GMC000396 GMC99021 A. tumefaciens. pAL4404, pSJG4 A. tumefaciens GMC000397 GMC99021 A. tumefaciens. pAL4404, pSSB1 A. tumefaciens GMC000398 GMC99021 A. tumefaciens. pAL4404, pUCD2714 A. tumefaciens GMC000399 GMC99021 A. tumefaciens. pTiB6S3SE A. tumefaciens GMC000400 GMC99021 A. tumefaciens. A. tumefaciens pTiB6S3SE/pMON120 (RC) GMC000401 GMC99021 A. tumefaciens. pTiC58 D:\401286708.doc A. tumefaciens Organism Description Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM30 Description: pCIBD10g containing AthCOR15a, a T-DNA / 266bp EcoRI-ScaI>CAMV-90>GUS FMB: 263 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pBKM31 Description: pCIBD10g containing AthCOR15a, a T-DNA / 233bp EcoRI-PmlI>CAMV-90>GUS FMB: 264 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pGLAM1 Description: pCIB10G containing AthCOR6.6, a T-DNA / CAMV35S>AthCOR6.6 sense FMB: 252 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pLCT71 Description: pCIBD10g containing AthCOR15a, a T-DNA / CAMV35S>AthCOR15a sense FMB: 271 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pSJG4 Description: pMON505 containing AthCOR6.6, a T-DNA / PMAS>AthCOR6.6 sense FMB: 253 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4404 Plasmid(s): pAL4404, pSSB1 Description: pBI101 containing AthCOR15a, a T-DNA / 973bp BglII-BglII>GUS FMB: 257 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA44404 Plasmid(s): pAL4404, pUCD2714 Description: pROK2 containing VfiLUXABfusion, a Binary T-DNA / LUX expression control FMB: 105 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: GV3111 Plasmid(s): pTiB6S3SE Description: containing , a Recipient strain for pMON cointegrate vectors FMB: 4 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: GV3111 Plasmid(s): pTiB6S3SE/pMON120 (RC) Description: Transformation Control without Kan resistance. FMB: 2 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Approval Code Application Code Common Name Taxonomic Name GMC000402 GMC99021 A. tumefaciens. pTiC58, pUCD1187 A. tumefaciens GMC000403 GMC99021 A. tumefaciens. pTiNE A. tumefaciens GMC000404 GMC99021 A. tumefaciens. pTiNE, pTi470 A. tumefaciens GMC000405 GMC99021 A. tumefaciens. pTiNE, pTi472 A. tumefaciens GMC000406 GMC99021 A. tumefaciens. pUCD2614 A. tumefaciens GMC000407 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pB129 GMC000408 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pB130 GMC000409 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pB162 GMC000410 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pJM06BB1 GMC000411 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens D:\401286708.doc Organism Description Organism: A. tumefaciens Strain: NTI Plasmid(s): pTiC58 Description: Wild type, oncogenic Agro FMB: 9 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pTiC58, pUCD1187 Description: Vir-B/LUX fusion for testing VIR induction. FMB: 17 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: GV3111 Plasmid(s): pTiNE Description: VIR plasmid. FMB: 10 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: GV3111 Plasmid(s): pTiNE, pTi470 Description: Binary T-DNA vector. FMB: 11 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: GV3111 Plasmid(s): pTiNE, pTi472 Description: Binary T-DNA vector. FMB: 12 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614 Description: VIR plasmid. FMB: 38 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pB129 Description: Binary T-DNA vector with NPTII FMB: 43 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pB130 Description: Binary T-DNA vector with NPTII and ONC FMB: 44 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pB162 Description: pB130 containing pTiC58-NOS, a Binary T-DNA vector with NPTII, NOS, & ONC FMB: 70 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pJM06BB1 Description: pUCD2711 containing AthBamy1, a T-DNA / CAMV35S-AthBamylase; antisense FMB: 214 Genesis Research and Development Corporation - Genetically Approval Code Application Code Common Name Taxonomic Name pJM11SL21 GMC000412 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pJM11SM9 GMC000413 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pLNB193 GMC000414 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pLNB194 GMC000415 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pLNB195 GMC000416 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pLNB196 GMC000417 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pU GMC000418 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD2713 GMC000419 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD2715 D:\401286708.doc Organism Description Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pJM11SL21 Description: pUCD2711 containing AthBamy1, a T-DNA / CAMV35S-AthBamylase; antisense FMB: 215 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pJM11SM9 Description: pUCD2711 containing AthBamy1, a T-DNA / CAMV35S-AthBamylase; sense FMB: 213 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pLNB193 Description: pUCD2706 containing StuAGPTSwt, a T-DNA / CAMV35S>StuAGPTSwt; sense FMB: 202 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pLNB194 Description: pUCD2706 containing StuAGPTSwt, a T-DNA / CAMV35S>StuAGPTSwt; antisense FMB: 203 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pLNB195 Description: pUCD2706 containing StuAGPTLwt, a T-DNA / CAMV35S>StuAGPTLwt; sense FMB: 204 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pLNB196 Description: pUCD2706 containing StuAGPTLwt, a T-DNA / CAMV35S>StuAGPTLwt; anitsense FMB: 205 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3635 Description: pUCD3629 containing LesPAL1, a Binary T-DNA / 807bpPPAL>omega>GUS FMB: 166 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD2713 Description: pUCD2706 containing VfiLUXABfusion, a Binary TDNA / LUX expression control FMB: 104 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD2715 Description: pUCD2711 containing VfiLUXABfusion, a Binary TDNA / omega>LUX expression control FMB: 106 Approval Code GMC000420 Application Code GMC99021 GMC000421 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD31 GMC000422 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD3625 GMC000423 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD3626 GMC000424 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD3628 GMC000425 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD3629 GMC000426 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD3633 GMC000427 GMC99021 A. tumefaciens. pUCD2614, A. tumefaciens pUCD3634 GMC000428 GMC99021 A. tumefaciens. pUCD510 A. tumefaciens GMC000429 GMC99021 A. tumefaciens. VIR plasmid, pKSW101.1BS A. tumefaciens D:\401286708.doc Common Name Taxonomic Name A. tumefaciens. pUCD2614, A. tumefaciens pUCD2716 Organism Description Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD2716 Description: pUCD2711 containing GUS, a Binary T-DNA / omega>GUS expression control FMB: 107 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD31 Description: pB129 containing pTiC58-NOS, a Binary T-DNA vector with NPTII and NOS FMB: 69 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3625 Description: Binary T-DNA / promoterless omega>LUX FMB: 153 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3626 Description: pUCD3625 containing LesPAL1, a PAL 1.3kb promoter>omege>lux reporter T-DNA FMB: 154 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3628 Description: pUCD3627 containing GUS, a PAL 1.3kb promoter>omege>GUS T-DNA FMB: 155 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3629 Description: Binary T-DNA / promoterless omega>GUS FMB: 156 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3633 Description: pUCD3629 containing LesPAL1, a Binary T-DNA / 309bpPPAL>omega>GUS FMB: 164 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD2614, pUCD3634 Description: pUCD3629 containing LesPAL1, a Binary T-DNA / 432bpPPAL>omega>GUS FMB: 165 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: LBA4301wt Plasmid(s): pUCD510 Description: Cloning vector for Agrobacterium tumefaciens FMB: 73 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Approval Code Application Code Common Name Taxonomic Name GMC000430 GMC99021 A. tumefaciens. VIR plasmid, pKSW101NlaIII A. tumefaciens GMC000431 GMC99021 A. tumefaciens. VIR plasmid, pKSW121BglII A. tumefaciens GMC000432 GMC99021 E. coli pRK2013 Escherichia coli GMC000433 GMC99021 E. coli. AC70R1-504 E. coli. GMC000434 GMC99021 E. coli. AC70R1504/pMON17335 E. coli GMC000435 GMC99021 E. coli. AC70R1504/pMON17336 E. coli GMC000436 GMC99021 E. coli. AC70RI504/pMON17335, pMON17336 E. coli GMC000437 GMC99021 E. coli. BHB2688 E. coli GMC000438 GMC99021 E. coli. BHB2690 E. coli D:\401286708.doc Organism Description Organism: A. tumefaciens Strain: C58-CI Plasmid(s): VIR plasmid, pKSW101.1BS Description: pBI101 containing AthCOR15b, a T-DNA / 1kbp ScaIHindIII Cor15b>GUS FMB: 284 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: C58-CI Plasmid(s): VIR plasmid, pKSW101NlaIII Description: pBI101 containing AthCOR15b, a T-DNA / 300bp NlaIII-NlaIII Cor15b>GUS FMB: 285 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: A. tumefaciens Strain: C58-CI Plasmid(s): VIR plasmid, pKSW121BglII Description: pBI121 containing AthCOR15b, a T-DNA / CAMV35S>AthCOR15b sense FMB: 283 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pRK2013 Description: Conjugation helper plasmid. FMB: 36 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): Description: Cloning host, deficient in GlgC (AGP/Pyro) FMB: 180 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pMON17335 Description: pACYC(?) containing StuAGPTSwt, a Potato AGP tuber small, expression plasmid FMB: 185 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pMON17336 Description: pBR327(?) containing StuAGPTLwt, a Potato AGP tuber large, expression plasmid FMB: 186 . Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pMON17335, pMON17336 Description: StuAGP:ts-wt/tl-wt FMB: 187 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: BHB2688 Plasmid(s): Description: Lambda packaging extract strain A FMB: 86 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: BHB2690 Approval Code Application Code Common Name Taxonomic Name GMC000439 GMC99021 E. coli. CES200 E. coli GMC000440 GMC99021 E. coli. CES201 E. coli GMC000441 GMC99021 E. coli. CJ236 E. coli. GMC000442 GMC99021 E. coli. DB1317 E. coli GMC000443 GMC99021 E. coli. DH1 E. coli. GMC000444 GMC99021 E. coli. DH5-a E. coli. GMC000445 GMC99021 E. coli. DH5-a/pMON17335 E. coli GMC000446 GMC99021 E. coli. DH5-a/pMON17336 E. coli GMC000447 GMC99021 E. coli. DH5-a/pTZ18R D:\401286708.doc E. coli Organism Description Plasmid(s): Description: Lambda packaging extract strain B FMB: 87 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: CES200 Plasmid(s): Description: Cloning host for lambda phage for repetative genes (RecB-, RecC-, SbcB-, but McrB+) FMB: 85 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: CES201 Plasmid(s): Description: Cloning host for lambda phage for highly repetative genes (RecB-, RecC-, SbcB-, but McrB+) FMB: 90 . Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: CJ236 Plasmid(s): Description: Dut-, Ung-, Thi, RelA mutant for making uridylated ssDNA for Kunkel SDM FMB: 173 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DB1317 Plasmid(s): Description: Cloning host for lambda phage FMB: 95 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH1 Plasmid(s): Description: Cloning host FMB: 35 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): Description: Cloning host FMB: 50 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pMON17335 Description: pACYC(?) containing StuAGPTSwt, a Potato AGP tuber small, expression plasmid FMB: 181 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pMON17336 Description: pBR327(?) containing StuAGPTLwt, a Potato AGP tuber large, expression plasmid FMB: 182 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pTZ18R Description: Cloning vector Approval Code Application Code Common Name Taxonomic Name GMC000448 GMC99021 E. coli. DH5-a/pTZ19R E. coli GMC000449 GMC99021 E. coli. DH5-a/pUCD2706 E. coli GMC000450 GMC99021 E. coli. DH5-a/pUCD2711 E. coli GMC000451 GMC99021 E. coli. DH5a-MCR- E. coli. GMC000452 GMC99021 E. coli. G6MD3 E. coli. GMC000453 GMC99021 E. coli. G6MD3/pMON17335 E. coli GMC000454 GMC99021 E. coli. G6MD3/pMON17335, pMON17336 E. coli GMC000455 GMC99021 E. coli. G6MD3/pMON17336 E. coli GMC000456 GMC99021 E. coli. GM48 E. coli. GMC000457 GMC99021 E. coli. GM48/pUCD3623 E. coli D:\401286708.doc Organism Description FMB: 109 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pTZ19R Description: Cloning vector FMB: 110 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUCD2706 Description: Binary T-DNA vector / expression cassette FMB: 191 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUCD2711 Description: Binary T-DNA vector / omega expression cst FMB: 192 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5a-MCRPlasmid(s): Description: Cloning host, Mcr- methylation- strain FMB: 167 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): Description: Cloning host with a deletion of the entire Glg operon, requires DiAmmino Pimelic acid (DAP) FMB: 175 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): pMON17335 Description: pACYC(?) containing StuAGPTSwt, a Potato AGP tuber small, expression plasmid FMB: 183 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): pMON17335, pMON17336 Description: StuAGP:ts-wt/tl-wt FMB: 188 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): pMON17336 Description: pBR327(?) containing StuAGPTLwt, a Potato AGP tuber large, expression plasmid FMB: 184 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: GM48 Plasmid(s): Description: Cloning host, Dcm-, Dam- methylation- strain FMB: 142 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: GM48 Approval Code Application Code Common Name Taxonomic Name GMC000458 GMC99021 E. coli. GM48/pUCD3626 E. coli GMC000459 GMC99021 E. coli. HB101 E. coli. GMC000460 GMC99021 E. coli. HB101/pUCD1742 E. coli GMC000461 GMC99021 E. coli. HB101/pUCD2711 E. coli GMC000462 GMC99021 E. coli. HB101/pUCD607 E. coli GMC000463 GMC99021 E. coli. JM101 E. coli. GMC000464 GMC99021 E. coli. JM101/pTZ18R E. coli GMC000465 GMC99021 E. coli. JM101/pTZ19R E. coli GMC000466 GMC99021 E. coli. JM101/pUCD1742 E. coli D:\401286708.doc Organism Description Plasmid(s): pUCD3623 Description: pTZ18R containing LesPAL1, a PstI-AluI; 1.3kb PAL promoter to -3 of ATG start FMB: 152 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: GM48 Plasmid(s): pUCD3626 Description: pUCD3625 containing LesPAL1, a PAL 1.3kb promoter>omege>lux reporter T-DNA FMB: 145 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): Description: Cloning host FMB: 18 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD1742 Description: pUC118 containing GUS, a Promoterless-GUS cassette FMB: 135 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2711 Description: Binary T-DNA vector / omega expression cst FMB: 103 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD607 Description: pUCD5 containing VfiLUX, a Tet-LUX fusion for constitutive LUX in bacteria. FMB: 76 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): Description: Cloning host FMB: 111 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pTZ18R Description: Cloning vector FMB: 124 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pTZ19R Description: Cloning vector FMB: 125 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD1742 Description: pUC118 containing GUS, a Promoterless>GUS cassette FMB: 148 Approval Code GMC000467 Application Code GMC99021 E. coli. JM101/pUCD3623 E. coli GMC000468 GMC99021 E. coli. JM101/pUCD3626 E. coli GMC000469 GMC99021 E. coli. Lambda, cI857sam7 E. coli GMC000470 GMC99021 E. coli. LCB618 E. coli. GMC000471 GMC99021 E. coli. LCB618/pMON17335 E. coli GMC000472 GMC99021 E. coli. LCB618/pMON17335, pMON17336 E. coli GMC000473 GMC99021 E. coli. LCB618/pMON17336 E. coli GMC000474 GMC99021 E. coli. LE392 E. coli. GMC000475 GMC99021 E. coli. MM294 E. coli. GMC000476 GMC99021 E. coli. MV1193 E. coli. D:\401286708.doc Common Name Taxonomic Name Organism Description Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3623 Description: pTZ18R containing LesPAL1, a PstI-AluI; 1.3kb PAL promoter to -3 of ATG start FMB: 140 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3626 Description: pUCD3625 containing LesPAL1, a PAL 1.3kb promoter>omege>lux reporter T-DNA FMB: 144 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: C520 Plasmid(s): Lambda, cI857-sam7 Description: Lambda lysogen for production of lambda DNA for size standards FMB: 255 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: LCB618 Plasmid(s): Description: Cloning host, putative mutant for glgC FMB: 176 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: LCB618 Plasmid(s): pMON17335 Description: pACYC(?) containing StuAGPTSwt, a Potato AGP tuber small, expression plasmid FMB: 177 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: LCB618 Plasmid(s): pMON17335, pMON17336 Description: StuAGP:ts-wt/tl-wt FMB: 179 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: LCB618 Plasmid(s): pMON17336 Description: pBR327(?) containing StuAGPTLwt, a Potato AGP tuber large, expression plasmid FMB: 178 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: LE392 Plasmid(s): Description: Cloning host for lambda phage FMB: 83 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MM294 Plasmid(s): Description: Cloning host FMB: 13 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Approval Code Application Code Common Name Taxonomic Name GMC000477 GMC99021 E. coli. NW2 E. coli GMC000478 GMC99021 E. coli. P2-392 E. coli GMC000479 GMC99021 E. coli. pAB5 E. coli GMC000480 GMC99021 E. coli. pAP4 E. coli GMC000481 GMC99021 E. coli. pARK4 E. coli GMC000482 GMC99021 E. coli. pARK8 E. coli GMC000483 GMC99021 E. coli. pB129 E. coli GMC000484 GMC99021 E. coli. pB130 E. coli GMC000485 GMC99021 E. coli. pB160 E. coli D:\401286708.doc Organism Description Strain: MV1193 Plasmid(s): Description: F': traD36, ProAB, LacIqZ^M15/^lac-ProAB, Thi, SupE, ^(Srt-recA)306::Tn10 for Kunkel SDM FMB: 174 Also refer to FMB: 221, has same Strain & is the cloning host. Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: NW2 Plasmid(s): Description: Cloning host for lambda phage, McrFMB: 98 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: P2-392 Plasmid(s): Description: Cloning host for lambda phage with P2 lysogen FMB: 84 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pAB5 Description: VfiLUXABfusion, a Translational fusion of LUX A & B FMB: 101 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pAP4 Description: AthPAL4, a Arabidopsis phenylalanine ammonia lyase 4 FMB: 172 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pARK4 Description: Binary T-DNA vector with NOS FMB: 20 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pARK8 Description: Binary T-DNA vector with NPTII FMB: 21 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pB129 Description: Binary T-DNA vector with NPTII FMB: 31 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pB130 Description: Binary T-DNA vector with NPTII and ONC FMB: 32 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM109 Plasmid(s): pB160 Description: pTZ18R containing pTiC58-HindIII#23, a The T-DNA right border and NOS gene FMB: 39 Approval Code GMC000486 Application Code GMC99021 E. coli. pB161 E. coli Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM109 Plasmid(s): pB161 Description: pTZ18R containing pTiC58-NOS FMB: 68 GMC000487 GMC99021 E. coli. pB162 E. coli GMC000488 GMC99021 E. coli. pBGS130 E. coli GMC000489 GMC99021 E. coli. pBGS131 E. coli GMC000490 GMC99021 E. coli. pBGS18 E. coli GMC000491 GMC99021 E. coli. pBGS19 E. coli GMC000492 GMC99021 E. coli. pBI121 E. coli GMC000493 GMC99021 E. coli. pBKM17 E. coli Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pB162 Description: pB130 containing pTiC58-NOS, a Binary T-DNA vector with NPTII, NOS, & ONC FMB: 49 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBGS130 Description: pUC130 like Cloning vector with Km, not Ap FMB: 65 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBGS131 Description: pUC131 like Cloning vector with Km, not Ap FMB: 66 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBGS18 Description: pUC18 like Cloning vector with Km, not Ap FMB: 63 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBGS19 Description: pUC19 like Cloning vector with Km, not Ap FMB: 64 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pBI121 Description: Binary T-DNA / CAMV35S>GUS control FMB: 171 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM17 Description: pCIBD10g containing AthCOR15a, a T-DNA / 180bp PmlI-PmlI>CAMV-90>GUS FMB: 278 GMC000494 GMC99021 E. coli. pBKM18 E. coli GMC000495 GMC99021 E. coli. pBKM19 E. coli D:\401286708.doc Common Name Taxonomic Name Organism Description Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM18 Description: pCIBD10g containing AthCOR15a, a T-DNA / 498bp EcoRI-ClaI>CAMV-90>GUS FMB: 275 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Approval Code Application Code Common Name Taxonomic Name GMC000496 GMC99021 E. coli. pBKM25 E. coli GMC000497 GMC99021 E. coli. pBKM26 E. coli GMC000498 GMC99021 E. coli. pBKM27 E. coli GMC000499 GMC99021 E. coli. pBKM28 E. coli GMC000500 GMC99021 E. coli. pBKM30 E. coli GMC000501 GMC99021 E. coli. pBKM31 E. coli GMC000502 GMC99021 E. coli. pBKM32 E. coli GMC000503 GMC99021 E. coli. pBKM33 E. coli D:\401286708.doc Organism Description Organism: E. coli Strain: DH5-a Plasmid(s): pBKM19 Description: pCIBD10g containing CAMV-90, a T-DNA / CAMV90>GUS control FMB: 282 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM25 Description: pCIBD10g containing AthCOR15a, a T-DNA / C-Box 7mer MT50/51>CAMV-90>GUS FMB: 281 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM26 Description: pCIBD10g containing AthCOR15a, a T-DNA / 27bp C-Box MT50/51>CAMV-90>GUS FMB: 280 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM27 Description: pCIBD10g containing AthCOR15a, a T-DNA / mutCBox PmlI-PmlI>CAMV-90>GUS FMB: 279 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM28 Description: pBlueSK- containing MT50/MT51x6, a MT50/MT51 C-Box oligo 6mer FMB: 254 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM30 Description: pCIBD10g containing AthCOR15a, a T-DNA / 266bp EcoRI-ScaI>CAMV-90>GUS FMB: 276 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM31 Description: pCIBD10g containing AthCOR15a, a T-DNA / 233bp EcoRI-PmlI>CAMV-90>GUS FMB: 277 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM32 Description: pBlueSK- containing CAMV35S:-46, a CAMV35S:46>GUS>3C Promoter probe FMB: 272 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM33 Description: pBlueSK- containing CAMV35S:-46, a CAMV35S:46>GUS>3C Promoter probe FMB: 273 Approval Code GMC000504 Application Code GMC99021 E. coli. pBKM34 E. coli GMC000505 GMC99021 E. coli. pBlueKS+ E. coli GMC000506 GMC99021 E. coli. pBlueKS- E. coli GMC000507 GMC99021 E. coli. pBlueSK- Escherichia coli GMC000508 GMC99021 E. coli. pBR322 E. coli GMC000509 GMC99021 E. coli. pBR327 E. coli GMC000510 GMC99021 E. coli. pBR328 E. coli GMC000511 GMC99021 E. coli. pcB10ESIA E. coli GMC000512 GMC99021 E. coli. pCB25 E. coli GMC000513 GMC99021 E. coli. pCHI1 E. coli D:\401286708.doc Common Name Taxonomic Name Organism Description Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBKM34 Description: pBlueSK- containing CAMV35S:-90, a CAMV35S:90>GUS>3C Promoter probe FMB: 274 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBlueKS+ Description: Cloning vector FMB: 198 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pBlueKSDescription: Cloning vector FMB: 200 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1190 Plasmid(s): pBlueSKDescription: Cloning vector FMB: 199 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pBR322 Description: Cloning vector FMB: 51 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pBR327 Description: Tiny cloning vector FMB: 52 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pBR328 Description: Cloning vector with Ap, Tc, and Cm resistance FMB: 53 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pcB10ESIA Description: pEMBL12 containing NplATP2-1, a N. plumbaginafolia ATP synthase beta subunit FMB: 23 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pCB25 Description: pGEM4 containing LesADH2, a Tomato alcohol dehydrogenase cDNA FMB: 89 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Approval Code Application Code Common Name Taxonomic Name GMC000514 GMC99021 E. coli. pCHS1 E. coli GMC000515 GMC99021 E. coli. pCIB10 E. coli GMC000516 GMC99021 E. coli. pCIB710 E. coli GMC000517 GMC99021 E. coli. pCJ4 E. coli GMC000518 GMC99021 E. coli. pCP63.15 E. coli GMC000519 GMC99021 E. coli. pcPR1-h E. coli GMC000520 GMC99021 E. coli. pGLAM1 E. coli GMC000521 GMC99021 E. coli. pGLGCwt1 E. coli GMC000522 GMC99021 E. coli. pHH29dSK- E. coli D:\401286708.doc Organism Description Plasmid(s): pCHI1 Description: pSP65 containing PvuCHI1, a Bean chalcone isomerase cDNA FMB: 45 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pCHS1 Description: pBR325 containing PvuCHS1, a Bean chalcone synthase cDNA FMB: 46 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pCIB10 Description: Binary T-DNA vector with NPTII FMB: 259 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pCIB710 Description: Cloning vector w/ P-CAMV35S expression csst FMB: 258 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pCJ4 Description: Up mutant of E. coli GlgC (AGP,pyro) FMB: 197 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pCP63.15 Description: pUC9 containing StuPAL1, a Potato phenylalanine ammonia lyase cDNA FMB: 91 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pcPR1-h Description: pUC9 containing PcrPR1, a Celery pathogen response gene 1 cDNA FMB: 94 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pGLAM1 Description: pCIB10G containing AthCOR6.6, a T-DNA / CAMV35S>AthCOR6.6 sense FMB: 250 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pGLGCwt1 Description: pUC19 containing EcoGlgCwt, a E. coli GlgCwt in pUC19 FMB: 216 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1190 Plasmid(s): pHH29dSKDescription: pBlue containing AthCOR6.6, a Arabidopsis COR6.6 Approval Code Application Code Common Name Taxonomic Name GMC000523 GMC99021 E. coli. pHH72a E. coli GMC000524 GMC99021 E. coli. pHYP1 E. coli GMC000525 GMC99021 E. coli. pJAT3011 E. coli GMC000526 GMC99021 E. coli. pKa5 E. coli GMC000527 GMC99021 E. coli. pKG3 Escherichia coli GMC000528 GMC99021 E. coli. pLCT10A E. coli GMC000529 GMC99021 E. coli. pLCT61 Escherichia coli GMC000530 GMC99021 E. coli. pLCT71 E. coli GMC000531 GMC99021 E. coli. pLF15 E. coli D:\401286708.doc Organism Description cDNA FMB: 244 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pHH72dS919 Description: pMON919 containing AthCOR47, a PMAS>AthCOR47 cDNA expression cassette FMB: 247 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pHYP1 Description: pUC19 containing PvuHPRGP, a Bean hydroxyproline rich glycoprotein cDNA FMB: 47 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pJAT3011 Description: pUC? containing AthADH, a Arabidopsis Alcohol dehydrogenase FMB: 286 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pKa5 Description: pUC9 containing Stu4CL1, a Potato 4-coumarate lyase cDNA FMB: 92 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): pKG3 Description: Glg operon from up mutant CL1136 on a plasmid FMB: 209 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: XL1Blue Plasmid(s): pLCT10A Description: pBlue containing AthCOR15a, a Arabidopsis CORl15a cDNA FMB: 245 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLCT61 Description: pCIB710 containing AthCOR15a, a CAMV35S>AthCOR15a cDNA expression csst FMB: 248 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLCT71 Description: pCIBD10g containing AthCOR15a, a T-DNA / CAMV35S>AthCOR15a sense FMB: 270 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pLF15 Description: pBR322 containing PcrCHS1, a Celery chalcone Approval Code Application Code Common Name Taxonomic Name GMC000532 GMC99021 E. coli. pLNB189 E. coli GMC000533 GMC99021 E. coli. pLNB190 E. coli GMC000534 GMC99021 E. coli. pLNB193 E. coli GMC000535 GMC99021 E. coli. pLNB194 E. coli GMC000536 GMC99021 E. coli. pLNB195 E. coli GMC000537 GMC99021 E. coli. pLNB196 E. coli GMC000538 GMC99021 E. coli. pLNB206 E. coli GMC000539 GMC99021 E. coli. pLNB207 E. coli GMC000540 GMC99021 E. coli. pLNB223 E. coli D:\401286708.doc Organism Description synthase 1 cDNA FMB: 93 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB189 Description: pUC4 containing StuAGPTSwt, a Potato AGP tuber small, shuttle clone FMB: 189 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB190 Description: pUC4 containing StuAGPTLwt, a Potato AGP tuber large, shuttle clone FMB: 190 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB193 Description: pUCD2706 containing StuAGPTSwt, a T-DNA / CAMV35S>StuAGPTSwt; sense FMB: 193 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB194 Description: pUCD2706 containing StuAGPTSwt, a T-DNA / CAMV35S>StuAGPTSwt; antisense FMB: 194 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB195 Description: pUCD2706 containing StuAGPTLwt, a T-DNA / CAMV35S>StuAGPTLwt; sense FMB: 195 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB196 Description: pUCD2706 containing StuAGPTLwt, a T-DNA / CAMV35S>StuAGPTLwt; anitsense FMB: 196 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB206 Description: pUCD3629, a Binary T-DNA / promoterless omega>GUS FMB: 206 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB207 Description: pUCD3629, a Binary T-DNA / promoterless omega>GUS FMB: 207 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB223 Approval Code Application Code Common Name Taxonomic Name GMC000541 GMC99021 E. coli. pLNB224 E. coli GMC000542 GMC99021 E. coli. pLNB227 E. coli GMC000543 GMC99021 E. coli. pLNB228 E. coli GMC000544 GMC99021 E. coli. pLNB229 E. coli GMC000545 GMC99021 E. coli. pLNB230 E. coli GMC000546 GMC99021 E. coli. pLNB231 E. coli GMC000547 GMC99021 E. coli. pLNB232 E. coli GMC000548 GMC99021 E. coli. pLNB232, pMON17336 E. coli GMC000549 GMC99021 E. coli. pLNB233 E. coli D:\401286708.doc Organism Description Description: pTZ18R containing StuAGPTSwt, a Potato AGP tuber small, phagmid clone FMB: 223 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB224 Description: pT7T3-18U containing StuAGPTSwt, a Potato AGP tuber small, for SDM FMB: 224 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB227 Description: pT7T3-18U containing StuAGPTS:K188E, a Potato AGP tuber small, SDM: K188E FMB: 227 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB228 Description: pT7T3-18U containing StuAGPTS:K188A, a Potato AGP tuber small, SDM: K188A FMB: 228 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB229 Description: pT7T3-19U containing StuAGPTLwt, a Potato AGP tuber large, for SDM FMB: 229 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB230 Description: pTZ18R containing LesPAL1, a EcoRI 0.58kb frag; edit site; PCR #2 from cDNA FMB: 230 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB231 Description: pT7T3-18U containing StuAGPTS:K431E, a Potato AGP tuber small, SDM: K431E FMB: 231 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB232 Description: pACYC(?) containing StuAGPTS:K188E, a Potato AGP tuber small, K188E expression FMB: 232 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pLNB232, pMON17336 Description: StuAGP:ts-K188E/tl-wt expression system FMB: 234 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB233 Approval Code Application Code Common Name Taxonomic Name GMC000550 GMC99021 E. coli. pLNB233, pMON17336 E. coli GMC000551 GMC99021 E. coli. pLNB236 E. coli GMC000552 GMC99021 E. coli. pLNB237 E. coli GMC000553 GMC99021 E. coli. pLNB237, pMON17336 E. coli GMC000554 GMC99021 E. coli. pLNB238 E. coli GMC000555 GMC99021 E. coli. pLNB238, pMON17336 E. coli GMC000556 GMC99021 E. coli. pLNB241 E. coli GMC000557 GMC99021 E. coli. pLNB242 E. coli GMC000558 GMC99021 E. coli. pLNB243 E. coli D:\401286708.doc Organism Description Description: pACYC(?) containing StuAGPTS:K188A, a Potato AGP tuber small, K188A expression FMB: 233 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pLNB233, pMON17336 Description: StuAGP:ts-K188A/tl-wt expression system FMB: 235 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB236 Description: pT7T3-18U containing StuAGPTS:K431A, a Potato AGP tuber small, SDM: K431A FMB: 236 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB237 Description: pACYC(?) containing StuAGPTS:K431E, a Potato AGP tuber small, K431E expression FMB: 237 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pLNB237, pMON17336 Description: StuAGP:ts-K431E/tl-wt expression system FMB: 239 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB238 Description: pACYC(?) containing StuAGPTS:K431A, a Potato AGP tuber small, K431A expression FMB: 238 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: AC70R1-504 Plasmid(s): pLNB238, pMON17336 Description: StuAGP:ts-K431A/tl-wt expression system FMB: 240 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB241 Description: pBlueKS- containing AthCOR15a, a PmlI-PmlI 180bp promoter fragment FMB: 241 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pLNB242 Description: pBlueKS- containing AthCOR15a, a ScaI-ClaI 230bp promoter fragment FMB: 242 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB243 Description: pBlueKS- containing AthCOR15a, a EcoRI-HindIII 600bp promoter fragment Approval Code Application Code Common Name Taxonomic Name GMC000559 GMC99021 E. coli. pLNB256 E. coli GMC000560 GMC99021 E. coli. pLNB74 Escherichia coli GMC000561 GMC99021 E. coli. pMON200 E. coli GMC000562 GMC99021 E. coli. pMON316 Escherichia coli GMC000563 GMC99021 E. coli. pMON505 E. coli GMC000564 GMC99021 E. coli. pMON919 E. coli GMC000565 GMC99021 E. coli. pOP12 E. coli GMC000566 GMC99021 E. coli. pPAL5 E. coli GMC000567 GMC99021 E. coli. pPD5 E. coli GMC000568 GMC99021 E. coli. pRK2013 E. coli D:\401286708.doc Organism Description FMB: 243 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pLNB256 Description: pBlueSK- containing MT50/MT51x6, a MT50/MT51 C-Box oligo 6mer; EcoRI fragment FMB: 256 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pLNB74 Description: Cloning vector with Tc resistance only FMB: 74 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MM294 Plasmid(s): pMON200 Description: Cointegrate T-DNA vector. FMB: 15 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MM294 Plasmid(s): pMON316 Description: Binary T-DNA vector. FMB: 16 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pMON505 Description: Binary T-DNA vector with NPTII FMB: 260 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pMON919 Description: Cloning vector w/ P-MAS expression cassette FMB: 261 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): pOP12 Description: Glg operon on a plasmid FMB: 212 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pPAL5 Description: pAT153 containing PvuPAL5, a Bean phenylalanine ammonia lyase cDNA FMB: 75 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: G6MD3 Plasmid(s): pPD5 Description: Glg operon on a plasmid FMB: 210 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Approval Code Application Code Common Name Taxonomic Name GMC000569 GMC99021 E. coli. pROK2 E. coli GMC000570 GMC99021 E. coli. pSHS78 E. coli GMC000571 GMC99021 E. coli. pSJG4 E. coli GMC000572 GMC99021 E. coli. pSUP5011 E. coli GMC000573 GMC99021 E. coli. pT7T3-18U E. coli GMC000574 GMC99021 E. coli. pT7T3-19U E. coli GMC000575 GMC99021 E. coli. pTA71 E. coli GMC000576 GMC99021 E. coli. pTCHS1 E. coli GMC000577 GMC99021 E. coli. pTCHS2 E. coli D:\401286708.doc Organism Description Strain: MM294 Plasmid(s): pRK2013 Description: Conjugation helper plasmid. FMB: 14 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pROK2 Description: Binary T-DNA vector / expression cassette FMB: 100 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pSHS78 Description: pBlue containing AthCOR78, a Arabidopsis COR78 cDNA FMB: 249 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pSJG4 Description: pMON505 containing AthCOR6.6, a T-DNA / PMAS>AthCOR6.6 sense FMB: 251 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: S17-1 Plasmid(s): pSUP5011 Description: Tn5 with Km resistance, suicide vector FMB: 37 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pT7T3-18U Description: Cloning vector FMB: 225 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pT7T3-19U Description: Cloning vector FMB: 226 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH1 Plasmid(s): pTA71 Description: pACYC184 containing Tae-rRNA, a Wheat ribosomal gene cluster FMB: 22 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pTCHS1 Description: pBlueKS+ containing LesCHS1, a Tomato chalcone synthase 1 cDNA FMB: 79 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: ? Plasmid(s): pTCHS2 Description: pBlueKS+ containing LesCHS1, a Tomato chalcone synthase 2 cDNA Approval Code Application Code Common Name Taxonomic Name GMC000578 GMC99021 E. coli. pTPAL1S E. coli GMC000579 GMC99021 E. coli. pTZ19U E. coli GMC000580 GMC99021 E. coli. pUC119 E. coli GMC000581 GMC99021 E. coli. pUC128 E. coli GMC000582 GMC99021 E. coli. pUC129 E. coli GMC000583 GMC99021 E. coli. pUC19 E. coli GMC000584 GMC99021 E. coli. pUC4 E. coli GMC000585 GMC99021 E. coli. pUC8 E. coli GMC000586 GMC99021 E. coli. pUCD1571 E. coli GMC000587 GMC99021 E. coli. pUCD1731 E. coli D:\401286708.doc Organism Description FMB: 80 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pTPAL1S Description: pTZ18R containing LesPAL1, a SalI 2kb fragment ? FMB: 112 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pTZ19U Description: Cloning vector (& HpaII low molecular wt. std) FMB: 160 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: MV1193 Plasmid(s): pUC119 Description: Cloning vector FMB: 222 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUC128 Description: Cloning vector FMB: 61 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUC129 Description: Cloning vector FMB: 62 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUC19 Description: Cloning vector FMB: 55 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUC4 Description: Cloning vector with palindromic polylinker FMB: 54 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUC8 Description: Cloning vector FMB: 67 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH1 Plasmid(s): pUCD1571 Description: pUCD615 containing Atu-pscA FMB: 72 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD1731 Description: VfiLUXABfusion, a Trp>omega>LUX for constitutive LUX in bacteria Approval Code Application Code Common Name Taxonomic Name GMC000588 GMC99021 E. coli. pUCD2 E. coli GMC000589 GMC99021 E. coli. pUCD2-11K1 E. coli GMC000590 GMC99021 E. coli. pUCD2-3KS E. coli GMC000591 GMC99021 E. coli. pUCD2335 E. coli GMC000592 GMC99021 E. coli. pUCD2706 E. coli GMC000593 GMC99021 E. coli. pUCD2715 E. coli GMC000594 GMC99021 E. coli. pUCD2716 E. coli GMC000595 GMC99021 E. coli. pUCD2720 E. coli GMC000596 GMC99021 E. coli. pUCD3 E. coli GMC000597 GMC99021 E. coli. pUCD31 E. coli D:\401286708.doc Organism Description FMB: 108 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2 Description: Broad host cloning vector with Sp, Km, Ap, & Tc FMB: 56 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2-11K1 Description: pUCD2 containing pTiC58-KpnI#11, a Vir G, Vir C, and part of Vir D FMB: 40 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2-3KS Description: pUCD2 containing pTiC58-KpnI#3, a The entire pTiC58 T-DNA in pUCD2 FMB: 34 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUCD2335 Description: Binary T-DNA vector with HPH and COS FMB: 136 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2706 Description: Binary T-DNA vector / expression cassette FMB: 102 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2715 Description: Binary T-DNA / CAMV35S>omega>LUX control FMB: 150 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2716 Description: Binary T-DNA / CAMV35S>omega>GUS control FMB: 151 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD2720 Description: Binary T-DNA / promoterless omega>LUX FMB: 139 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD3 Description: Broad host cloning vector with Cm, Km, & COS FMB: 57 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD31 Approval Code Application Code Common Name Taxonomic Name GMC000598 GMC99021 E. coli. pUCD310 E. coli GMC000599 GMC99021 E. coli. pUCD3600 E. coli GMC000600 GMC99021 E. coli. pUCD3601 E. coli GMC000601 GMC99021 E. coli. pUCD3602 E. coli GMC000602 GMC99021 E. coli. pUCD3603 E. coli GMC000603 GMC99021 E. coli. pUCD3604 E. coli GMC000604 GMC99021 E. coli. pUCD3605 E. coli GMC000605 GMC99021 E. coli. pUCD3606 E. coli GMC000606 GMC99021 E. coli. pUCD3607 E. coli D:\401286708.doc Organism Description Description: pB129 containing pTiC58-NOS, a Binary T-DNA vector with NPTII and NOS FMB: 48 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD310 Description: pUCD5 containing VfiLUX, a Tet-LUX fusion for constitutive LUX in bacteria. FMB: 3 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUCD3600 Description: pUC19 containing pTiC58-XbaI#7 FMB: 71 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3601 Description: pTZ18R containing LesPAL1, a SalI 9.1kb fragment FMB: 113 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3602 Description: pTZ18R containing LesPAL1, a SalI 3.7kb fragment orientation 1 FMB: 114 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3603 Description: pTZ18R containing LesPAL1, a SalI 3.7kb fragment orientation 2 FMB: 115 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3604 Description: pTZ18R containing LesPAL1, a EcoRI 3.5kb frag orient 1; 3' untranscribed region FMB: 116 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3605 Description: pTZ18R containing LesPAL1, a EcoRI 3.5kb frag orient 2; 3' untranscribed region FMB: 117 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3606 Description: pTZ18R containing LesPAL1, a EcoRI-SalI 2.0kb frag; promoter - exon I - intron FMB: 118 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3607 Description: pTZ18R containing LesPAL1, a EcoRI-SalI 1.4kb Approval Code Application Code Common Name Taxonomic Name GMC000607 GMC99021 E. coli. pUCD3608 E. coli GMC000608 GMC99021 E. coli. pUCD3609 E. coli GMC000609 GMC99021 E. coli. pUCD3610 E. coli GMC000610 GMC99021 E. coli. pUCD3611 E. coli GMC000611 GMC99021 E. coli. pUCD3612 E. coli GMC000612 GMC99021 E. coli. pUCD3613 E. coli GMC000613 GMC99021 E. coli. pUCD3614 E. coli GMC000614 GMC99021 E. coli. pUCD3615 E. coli GMC000615 GMC99021 E. coli. pUCD3616 E. coli D:\401286708.doc Organism Description frag; 3' distal region FMB: 119 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3608 Description: pTZ18R containing LesPAL1, a EcoRI 0.85kb frag orient 1; 3' end of gene FMB: 120 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3609 Description: pTZ18R containing LesPAL1, a EcoRI 0.85kb frag orient 2; 3' end of gene FMB: 121 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3610 Description: pTZ18R containing LesPAL1, a EcoRI 0.54kb frag orient 1; 5' end of exon II FMB: 122 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3611 Description: pTZ18R containing LesPAL1, a EcoRI 0.58kb frag orient 1; exon II center/edit site FMB: 123 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3612 Description: pTZ18R containing LesPAL1, a BamHI 7.3kb frag orient 1; 3' end of gene FMB: 126 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3613 Description: pTZ18R containing LesPAL1, a BamHI 7.3kb frag orient 1; 3' end of gene FMB: 127 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3614 Description: pTZ19R containing LesPAL1, a EcoRI-SalI 1.4kb frag; 3' distal region FMB: 128 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3615 Description: pTZ19R containing LesPAL1, a EcoRI 0.85kb frag orient 1; 3' end of gene FMB: 129 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3616 Approval Code Application Code Common Name Taxonomic Name GMC000616 GMC99021 E. coli. pUCD3617 E. coli GMC000617 GMC99021 E. coli. pUCD3618 E. coli GMC000618 GMC99021 E. coli. pUCD3619 E. coli GMC000619 GMC99021 E. coli. pUCD3620 E. coli GMC000620 GMC99021 E. coli. pUCD3621 E. coli GMC000621 GMC99021 E. coli. pUCD3622 Escherichia coli GMC000622 GMC99021 E. coli. pUCD3624 E. coli GMC000623 GMC99021 E. coli. pUCD3625 E. coli GMC000624 GMC99021 E. coli. pUCD3627 E. coli D:\401286708.doc Organism Description Description: pTZ19R containing LesPAL1, a EcoRI 0.85kb frag orient 2; 3' end of gene FMB: 130 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3617 Description: pTZ18R containing LesPAL1, a PstI 2.0kb frag orient 1; 3' end of gene FMB: 131 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3618 Description: pTZ18R containing LesPAL1, a PstI 2.0kb frag orient 2; 3' end of gene FMB: 132 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3619 Description: pTZ18R containing LesPAL1, a PstI 3.3kb frag orient 1; 5' end/most of gene FMB: 133 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3620 Description: pTZ18R containing LesPAL1, a PstI 3.3kb frag orient 2; 5' end/most of gene FMB: 134 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3621 Description: pTZ18R containing LesPAL1, a EcoRI 0.54kb frag orient 2; 5' end of exon II FMB: 137 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: GM48 Plasmid(s): pUCD3622 Description: pTZ18R containing LesPAL1, a PstI-plus285 ExoIII; promoter & exonic element FMB: 138 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUCD3624 Description: pUCD2720 containing LesPAL1, a PAL 1.3kb promoter>omege>lux reporter T-DNA FMB: 141 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: DH5-a Plasmid(s): pUCD3625 Description: Binary T-DNA / promoterless omega>LUX FMB: 143 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3627 Approval Code Application Code Common Name Taxonomic Name GMC000625 GMC99021 E. coli. pUCD3628 E. coli GMC000626 GMC99021 E. coli. pUCD3629 E. coli GMC000627 GMC99021 E. coli. pUCD3630 E. coli GMC000628 GMC99021 E. coli. pUCD3631 E. coli GMC000629 GMC99021 E. coli. pUCD3632 E. coli GMC000630 GMC99021 E. coli. pUCD3633 E. coli GMC000631 GMC99021 E. coli. pUCD3634 E. coli GMC000632 GMC99021 E. coli. pUCD3635 E. coli GMC000633 GMC99021 E. coli. pUCD3636 E. coli D:\401286708.doc Organism Description Description: pUCD3625 containing LesPAL1, a Binary T-DNA / PPAL>omega expression cst FMB: 146 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3628 Description: pUCD3627 containing GUS, a PAL 1.3kb promoter>omege>GUS T-DNA FMB: 147 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3629 Description: Binary T-DNA / promoterless omega>GUS FMB: 149 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3630 Description: pTZ18R containing LesPAL1, a SpeI-AluI; 309bp PAL promoter to -3 of ATG start FMB: 157 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3631 Description: pTZ18R containing LesPAL1, a BglII-AluI; 432bp PAL promoter to -3 of ATG start FMB: 158 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3632 Description: pTZ18R containing LesPAL1, a BclI-AluI; 807bp PAL promoter to -3 of ATG start FMB: 159 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3633 Description: pUCD3629 containing LesPAL1, a Binary T-DNA / 309bpPPAL>omega>GUS FMB: 161 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3634 Description: pUCD3629 containing LesPAL1, a Binary T-DNA / 432bpPPAL>omega>GUS FMB: 162 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3635 Description: pUCD3629 containing LesPAL1, a Binary T-DNA / 807bpPPAL>omega>GUS FMB: 163 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3636 Approval Code Application Code Common Name Taxonomic Name GMC000634 GMC99021 E. coli. pUCD3637 E. coli GMC000635 GMC99021 E. coli. pUCD3638 E. coli GMC000636 GMC99021 E. coli. pUCD5 E. coli GMC000637 GMC99021 E. coli. pUCD623 E. coli GMC000638 GMC99021 E. coli. pUCD9P E. coli GMC000639 GMC99021 E. coli. pUCD9X E. coli GMC000640 GMC99021 E. coli. RR1 E. coli GMC000641 GMC99021 E. coli. VCS257 Escherichia coli GMC000642 GMC99021 E. coli. XL1Blue E. coli. GMC000643 GMC99021 E.coli. DB1318 E. coli D:\401286708.doc Organism Description Description: pTZ19R containing LesPAL1, a EcoRI 0.58kb frag; edit site; PCR from genome FMB: 168 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3637 Description: pTZ19R containing LesPAL1, a EcoRI 0.58kb frag; edit site; PCR #1 from cDNA FMB: 169 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: JM101 Plasmid(s): pUCD3638 Description: pTZ19R containing NPTII, a Promoterless NPTII gene as a SalI frag FMB: 170 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD5 Description: Broad host cloning vector w/ Km, Ap, Tc, & COS FMB: 58 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD623 Description: Promotorless Tn-LUX (TnLUX) FMB: 77 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD9P Description: Cloning vector FMB: 59 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: HB101 Plasmid(s): pUCD9X Description: Cloning vector FMB: 60 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: RR1 Plasmid(s): Description: Cloning host FMB: 19 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: VCS257 Plasmid(s): Description: Cloning host for lambda phage FMB: 97 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Strain: XL1Blue Plasmid(s): Description: Cloning host FMB: 201 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: E. coli Approval Code Application Code Common Name Taxonomic Name GMC000644 GMC99021 Lambda. bAt1001 Lambda GMC000645 GMC99021 Lambda. EMBL3 Lambda. GMC000646 GMC99021 Lambda. fAt1504 Lambda GMC000647 GMC99021 Lambda. fAt3101 Lambda GMC000648 GMC99021 Lambda. LesPAL1 Lambda GMC000649 GMC99021 X. campestris. 6D53-R X. campestris GMC000650 GMC99021 X. campestris. pUCD607 X. campestris GMC000651 GMC99021 X. campestris. pUCD623 X. campestris D:\401286708.doc Organism Description Strain: DB1318 Plasmid(s): Description: Cloning host for lambda phage FMB: 96 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: Lambda Strain: SEP6 Plasmid(s): bAt1001 Description: SEP6 containing AthCAB, a Arabidopsis Chlorophyll binding protein FMB: 26 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: Lambda Strain: EMBL3 Plasmid(s): Description: Lambda cloning vector FMB: 88 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: Lambda Strain: EMBL4 Plasmid(s): fAt1504 Description: EMBL4 containing AthSSP, a Arabidopsis seed storage protein FMB: 25 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: Lambda Strain: EMBL4 Plasmid(s): fAt3101 Description: EMBL4 containing AthADH, a Arabidopsis Alcohol dehydrogenase FMB: 24 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: Lambda Strain: EMBL3 Plasmid(s): LesPAL1 Description: EMBL3 containing LesPAL1, a Tomato phenylalanine ammonia lyase genomic FMB: 99 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: X. campestris Strain: 6D53-R Plasmid(s): Description: Xanthamonas campestris vesicatoria FMB: 78 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: X. campestris Strain: 6D53-R Plasmid(s): pUCD607 Description: Tet-LUX fusion for constitutive LUX in bacteria. FMB: 81 Genesis Research and Development Corporation - Genetically Modified Microorganisms Collection, Risk Group 1 (GMC99021) Organism: X. campestris Strain: 6D53-R Plasmid(s): pUCD623 Description: Promotorless Tn-LUX (TnLUX) FMB: 82