Load organisms from green house. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Click “open” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Select an organism for self cross for eg. The Red organism Then click tab “Self-cross One Organism” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Resulting offsprings of Red organism. Then double click on Tray1 and click “Send to Lower Panel” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Select two different organisms(heterozygous) Then click the tab “Cross Two Organisms” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Resulting offsprings of Red and Green-1 organism. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Select two same organisms(homozygous) Then click the tab “Cross two Organisms” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Resulting offsprings of Red organism. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Select an organism Then click “Mutate One Organism” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Mutant versions of Green-2 organism. Then click the tab “Biochemistry”. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON In the Biochemistry add amino acid sequence SSSSSSS here. History List is populated with corresponding template and phenotype.-White organism Then click “FOLD” The default phenotype Corresponding phenotype populated when sequence is folded. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Enter another sequence FFFFFFF and click “FOLD” Heterozygous combined phenotype(Red) Corresponding phenotype(Red) 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Enter the same sequence FFFFFFF here and click “FOLD” Homozygous combined phenotype.(Red ) 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Then click tab “Molecular Biology” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Click the tab “Enter New DNA” Then enter the DNA sequenceTATAAATGTCTAATCGTCATATTTT ATTAGTTTTTTGTCGTCAATAGGGGGG and click “OK” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Then click “Fold Protein” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Resulting Organism -Red Default Phenotype 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Double click “History List” and select “Send to Lower Panel” or Enter the same DNA by clicking “Enter New DNA” in Lower panel . 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON The same two DNA sequences give the combined phenotype here(Red) which is homozygous. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Enter DNACAGCTATAACCGAGATTGATGTCTAGTGCGATAAGCCCCAAAGATCGGCACATTTTGTG CGCTATACAAAGGTTAGTGTACTGGCGGCAGTAGTAGGGGGCGT and click “OK” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Click “Fold Protein” 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON Resulting Green-1 organism The two different DNA sequences gives a Heterozygous combined phenotype which populates here. 24 April, 2008 MGX TUTORIAL MGX © 2007-2008 UMASS BOSTON