A
3.5
3.8
9.33 13.3 13.5 11.1 9.32 13.1 13.7
9.8
WT
12
21226
1375
947/ 831
564
B
21226
1375
947/ 831
564
Fig. 1A PCR analyses demonstrating the presence of the nptII (A) and StSy (B) genes in
transformed banana plants. Lanes 1-9: different transformed plants; lane 10: plasmid
pYC39 (positive control); lane 11: a wild-type control; lane 12:  EcoRI + HindIII size
markers.
1
Fig. 1B Southern hybridization analysis of DNA from five transformed banana plants.
Genomic DNA was digested with HindIII. The membrane was hybridized with the 700bp nptII coding region. Con—non-transformed control plant.
2
Table 1 Germination of Botrytis cinerea conidia in transgenic banana leaf extracts
Banana
Germination
Germ-tube length
a
line
(%)
(μm)a
13.1
23b
2.33b
9.33
14.8a
1.56a
11.3
28b
2.76b
11.6
24.4b
2.48b
11.1
27.4b
2.44b
Control I
62c
6.52c
Control II
59.4c
5.96cd
a
Values followed by the same letter are not significantly different at p  0.05, (Tukey,
1953)
3
Table 2 Botrytis cinerea leaf spot severity in different transgenic banana lines
Exp 1
Banana
line
9.33
13.1
13.7
9.3
11.3
11.4
11.6
11.4
11.1
3.8
31.1
33.2
Control
LSD
Exp 2
Banana
line
Incubation time (days)
8
14
18
6.3
7.1*
7.1*
8.8
12.1
12.1
5.3
11.4
12.1
2.5*
4.2*
3.3*
4.2*
5.4*
5.4*
5
9.2
8.7
2.5*
4.2*
4.2*
0*
11.7
11.7
0*
0*
0*
7.5
13.3
15
1.7*
1.7*
1.7*
6.4
11.3
14.2
9.8
8.7
9.2
0.36
0.92
0.95
9.33
13.1
13.7
9.3
11.3
11.4.
11.6
11.4
11.1
3.8
31.1
33.2
Control
LSD
Incubation time (days)
4
8
12
5*
14.2*
15*
15.8
17.5
20
10*
20
22.5
17.5
24.2
27.5
7.9*
15.8*
16.7*
13.8
15.8*
28.3
10.4*
29.2
57.1
13.8
15*
15.8*
0*
7.9*
8.3*
9.2*
15.8*
15.8*
7.9*
19.7
20.8
7.4*
16.2*
16.7*
13.3
18.3
21.7
1.2
1.4
1.9
* Significantly reduced disease severity as compared with the wild type control.
4
Table 3. Transgenic banana lines producing equal or greater number
of flowers as compared to the conventional control 'Grand Nain'.
TRANSGENIC
LINE
CODE
FLOWERS
PER 8
POSITIONS
QTY.
DIFFERENCE
CONTROLS a
+ FACTOR
DIFFERENCE
CONTROLS
%
DIFFERENCE
CONTROLS
9.32
11.8
9.8
9.33
3.5
11.1
3.8
13.1
13.5
13.3
8
10
7
10
6
12
6
5
5
8
+3
+5
+2
+5
+1
+7
+1
0
0
+3
1.6
2.0
1.4
2.0
1.2
2.4
1.2
0.0
0.0
1.6
+ 60 %
+ 100%*
+ 40%
+ 100%*
+20%
+140%*
+ 20%
0%
0%
+ 60%
* Flower numbers are statistically greater than the controls; least square means of 5
flowers from 8 plants per line compared using Fisher’s protected least significant
difference (FLSD)
a
Number of flowers equal to or greater than the controls
5