OLLSCOIL NA hEIREANN, GAILLIMH SUMMER EXAMINATIONS 1999 SECOND SCIENCE MICROBIOLOGY FIRST PAPER EXTERN EXAMINER Professor J Smith INTERN EXAMINER Internal Examiners TIME ALLOWED: 3 hours ANSWER FIVE QUESTIONS (INDICATE CLEARLY THE QUESTIONS ANSWERED ON THE FIRST PAGE OF YOUR ANSWER BOOK) 1. Write an essay entitled "Gene Transciption" detailing the enzymes involved, gene promoter structure, and how transciption is terminated at the end of a gene. 2. Write an essay describing bacterial gene transfer by conjugation. Note one scientific application using conjugation. 3. Compare and contrast the Gram-positive and Gram-negative bacterial cell. 4. Write an essay on bacterial cell motility. 5. Compare and contrast the influences of temperature and pH on the growth of micro-organisms. 6. Discuss the techniques of batch culture and continuous culture. Include in your discussion an appraisal of their advantages and disadvantages, and suitable diagrams illustrating these techniques. 7. Define the term chemolithotrophy. Describe the mechanisms whereby chemolithotrophs obtain energy and reducing power for growth. 8. Outline the main characteristics of bacterial fermentation pathways. In your answer give examples of specific fermentation pathways.