5.1: Mendel’s Crosses and the Law of Segregation For example: the round seed allele is dominant. A plant with round seeds has at last one copy of the dominant allele. It can have one of these genotypes: homozygous dominant (RR) or heterozygous for the trait (Rr). A plant with wrinkled seeds can only be homozygous recessive (rr). Genes are found at specific locations (loci) on specific chromosomes. Alleles are alternative forms of a gene having the same position on a pair of homologous chromosomes. For example the “R” could represent allele for the dominant trait (round seeds) and the “r” represents the recessive allele (wrinkled seeds). Learning Check: 1. How do you know that a pea plant with wrinkled seeds does not have one copy of the round seed allele? 2. Add labels to the diagram, which illustrates Mendel’s first law. 1. B A 2. C 3. D 3. Use the diagram to write Mendel’s first law in your own words. 4. How can you remember what F2 stands for? 5. How can you remember the difference between genotype and phenotype? 5.1 & 5.2: One-Trait (Monohybrid) Crosses and Test Crosses A monohybrid cross involves parent organisms with different forms of one trait. Use a pattern similar to the one shown to the left to solve single trait (monohybrid) genetic questions Sometimes it is not possible to determine if an organism with a dominant phenotype is homozygous or heterozygous for that trait. A test cross is used to determine the genotype of the dominant phenotype organism by crossing it with a homozygous recessive phenotype. The results of the testcross will indicate the genotype of the parent with the dominant phenotype.