Genetic Engineerings Name: 1. Date: 4. Select the technique, chosen from the list below, that is best described by the statement shown. The diagram below represents a scienti c technique in use today. This technique involves the transfer of genetic information from a human to a bacterial cell such as E. coli, thus causing the bacterial cell to produce human growth hormone. A. Amniocentesis B. Cloning C. Genetic engineering D. Karyotyping 2. 3. Scientists have used this technique to In genetic engineering, the transfer of hereditary information from one species to another results in the formation of A. motile gametes B. C. dihybrids D. zygotes Select the term, chosen from the list below, that is best described by the statement shown. A portion of a chromosome is lost. Amniocentesis B. C. Cloning D. Deletion produce hormones for human use at a lower cost than other methods B. produce pathogens that are able to live in humans C. clone human cells with desired characteristics D. eliminate the need for laboratory production of medicines for humans recombinant DNA A. A. Karyotyping page 1 5. The diagram below represents a technique used in some molecular biology laboratories. Which phrase best describes a possible result of this process? A. the production of gametes having both human and bacterial DNA B. the production of a human hormone by the bacterial cell C. the introduction of a pathogen into a human cell D. the separation of DNA bacterial cell 6. ngerprints in the Which statement would most likely be used to describe the procedure represented in the diagram below? A. Enzymes are used to assemble an insulin gene, which is then attached to bacterial DNA. B. Bacterial DNA is cut from a human DNA strand and inserted into a human cell to form an insulin gene. C. The insulin gene is cut out of a human DNA strand using an enzyme and inserted into bacterial DNA, resulting in a combination of di erent DNA segments. D. A gene is deleted from bacterial DNA to produce an insulin gene, which is then inserted into human DNA. page 2 Genetic Engineerings 7. Which set of terms correctly identi es the procedure shown in the diagram below and a substance produced by this procedure? A. selective breeding—growth hormone B. cloning—antibiotics C. genetic engineering—insulin 9. The diagram below represents a genetic procedure. Which statement best describes the outcome of this procedure? D. replicating—glucose A. Bacterial cells will destroy defective human genetic material. B. Bacterial cells may form a multicellular embryo. C. The inserted human DNA will change harmful bacteria to harmless ones. D. The inserted human DNA may direct the synthesis of human proteins. 8. The diagram below represents a technique used in some molecular biology laboratories. 10. Base your answer(s) to the following question(s) on the diagram below, which illustrates some steps in genetic engineering and on your knowledge of biology. This technique is a type of A. chromatography B. gel electrophoresis C. direct harvesting D. genetic engineering State one way that enzymes are used in step 2. page 3 Genetic Engineerings 11. What is the result of step 3? A. a new type of molecular base is formed B. di erent types of minerals are joined together C. DNA from the bacterial cell is cloned 13. This change is most likely due to the cell being placed in D. DNA from di erent organisms is joined together 14. 12. A red onion cell has undergone a change, as represented in the diagram below. A. distilled water B. light C. salt water D. darkness The diagram below represents a common laboratory technique in molecular genetics. The ounder is a species of sh that can live in very cold water. The sh produces an “antifreeze” protein that prevents ice crystals from forming in its blood. The DNA for this protein has been identi ed. An enzyme is used to cut and remove this section of ounder DNA that is then spliced into the DNA of a strawberry plant. As a result, the plant can now produce a protein that makes it more resistant to the damaging e ects of frost. This process is known as A. sorting of genes B. genetic engineering C. recombination of chromosomes One common use of this technology is the D. mutation by deletion of genetic material A. production of a human embryo to aid women who are unable to have children B. change of single-celled organisms to multicellular organisms C. introduction of a toxic substance to kill bacterial cells D. production of hormones or enzymes to replace missing human body chemicals page 4 Genetic Engineerings 15. Researchers Cohn and Boyer transferred a gene from an African clawed frog into a bacterium. To accomplish this, these scientists had to use A. enzymes to cut out and insert the gene B. hereditary information located in amino acids C. radiation to increase the gene mutation rate of the bacterial cells 17. The accompanying diagrams represent some steps in a procedure used in biotechnology. Letters X and Y represent the D. cancer cells to promote rapid cell division A. hormones that stimulate the replication of bacterial DNA B. biochemical catalysts involved in the insertion of genes into other organisms C. hormones that trigger rapid mutation of genetic information D. gases needed to produce the energy required for gene manipulation 16. The production of certain human hormones by genetically engineered bacteria results from A. inserting a speci c group of amino acids into the bacteria B. combining a portion of human DNA with bacterial DNA and inserting this into bacteria C. crossing two di erent species of bacteria 18. The accompanying diagram represents a technique used in biotechnology. D. deleting a speci c amino acid from human DNA and inserting it into bacterial DNA Name a speci c substance that can be produced by this technique and state how humans have bene ted from the production of this substance. page 5 Genetic Engineerings 19. The gene for the production of human insulin is inserted into certain bacterial cells. The o spring of these bacterial cells will most likely be able to A. destroy pathogens B. reproduce sexually C. synthesize this hormone 21. D. form human tissue The accompanying diagram illustrates some key steps of a procedure in one area of biotechnology. The letter X most likely represents A. bacterial cells that are unable to synthesize insulin B. human cells that are able to synthesize antibodies C. bacterial cells that are able to synthesize insulin D. human cells that are unable to resist antibiotics 20. Many diabetics are now using insulin that was made by certain bacteria. The ability of these bacteria to produce insulin was most likely the result of A. deleting many DNA segments from bacterial DNA B. genetic mapping of bacterial DNA to activate the gene for insulin production C. inserting a portion of human DNA into the ring-shaped DNA of bacteria 22. A product of genetic engineering technology is represented below. Which substance was needed to join the insulin gene to the bacterial DNA as shown? D. using radiation to trigger mutations A. a speci c carbohydrate B. a speci c enzyme C. hormones D. antibodies page 6 Genetic Engineerings 23. Base your answer(s) to the following question(s) on the information below and on your knowledge of biology. 25. To demonstrate techniques used in DNA analysis, a student was given two paper strip samples of DNA. The two DNA samples are shown below. Base your answer(s) to the following question(s) on the information below and on your knowledge of biology. As part of a laboratory technique, DNA samples taken from four plants were separated. The results are represented in the diagram below. The student cut between the C and G in each of the shaded sequences in sample 1 and between the As in each of the shaded sequences in sample 2. Both sets of fragments were then arranged on a paper model of a gel. Identify the technique that was being demonstrated when the fragments were arranged on the gel model. Circle the band in the diagram below in the results for plant X that would contain the smallest DNA fragments. Support your answer. 24. The results of this type of DNA analysis are often used to help determine A. the number of DNA molecules in an organism B. if two species are closely related C. the number of mRNA molecules in DNA D. if two organisms contain carbohydrate molecules page 7 Genetic Engineerings Problem-Attic format version 4.4.263 c 2011–2015 EducAide Software _ Licensed for use by brian christopher Terms of Use at www.problem-attic.com Genetic Engineerings 04/13/2016 1. Answer: C 19. Answer: C 2. Answer: B 20. Answer: C 3. Answer: D 21. Answer: C 4. Answer: A 5. Answer: 22. Answer: B B 6. Answer: 23. Answer: electrophoresis or gel electrophoresis C 7. Answer: 24. Answer: B C 8. Answer: 25. Answer: D 9. Answer: D 10. Answer: Enzymes are used to cut the DNA. OR to cut the genetic material 11. Answer: D 12. Answer: B 13. Answer: C 14. Answer: D 15. Answer: A 16. Answer: B 17. Answer: B 18. Answer: This technique is used to produce insulin, which is used in the treatment of diabetes. or human growth hormone that can increase bone density in the elderly or hormones that may reduce the cost and side e ects of replacing missing body chemicals The smallest fragments travel the farthest/fastest in the gel.