Product datasheet
Anti-Isocitrate dehydrogenase antibody [GT1521]
ab184615
5 Images
Overview
Product name
Anti-Isocitrate dehydrogenase antibody [GT1521]
Description
Mouse monoclonal [GT1521] to Isocitrate dehydrogenase
Tested applications
ICC/IF, IP, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
Immunogen
Recombinant fragment within Human Isocitrate dehydrogenase aa 30-337. The exact sequence
is proprietary.
Database link: O75874
Positive control
293T, A431, HeLa, HepG2, PC-12, Rat2, Neuro2A, GL261, C8D30, NIH-3T3, Raw264.7 and
C2C12 whole cell extracts, A431 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Purity
Protein G purified
Clonality
Monoclonal
Clone number
GT1521
Isotype
IgG2b
Applications
Our Abpromise guarantee covers the use of ab184615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/100 - 1/1000.
IP
1/500 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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Target
Anti-Isocitrate dehydrogenase antibody [GT1521] images
Immunoprecipitaion analysis:
Lane1: 30 μg whole cell lysate/extract
of Isocitrate dehydrogenase expressing
HepG2 cells;
Lane 2: Control with 2.5 μg of pre-immune
mouse IgG;
Immunoprecipitation - Anti-Isocitrate
Lane 3: Immunoprecipitation of Isocitrate
dehydrogenase [GT1521] antibody (ab184615)
dehydrogenase protein by 2.5 μg of
ab184615.
The immunoprecipitated Isocitrate
dehydrogenase protein was detected by
western blot using ab184615 diluted at 1 :
1000.
Immunofluorescent analysis of ice-cold MeOH
fixed (for 5 min) A431 cells labeling Isocitrate
dehydrogenase at a 1/200 dilution (green),
with Hoechst 33342 counterstaining (blue).
Immunocytochemistry/ Immunofluorescence Anti-Isocitrate dehydrogenase antibody
(ab184615)
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All lanes : Anti-Isocitrate dehydrogenase
antibody [GT1521] (ab184615) at 1/1000
dilution
Lane 1 : Neuro2A whole cell lysate/extract
Lane 2 : GL261 whole cell lysate/extract
Lane 3 : C8D30 whole cell lysate/extract
Lane 4 : NIH-3T3 whole cell lysate/extract
Lane 5 : Raw264.7 whole cell lysate/extract
Lane 6 : C2C12 whole cell lysate/extract
Western blot - Anti-Isocitrate dehydrogenase
Lysates/proteins at 30 µg per lane.
antibody (ab184615)
Predicted band size : 46 kDa
10% SDS-PAGE
All lanes : Anti-Isocitrate dehydrogenase
antibody [GT1521] (ab184615) at 1/1000
dilution
Lane 1 : PC-12 whole cell lysate/extract
Lane 2 : Rat-2 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size : 46 kDa
Western blot - Anti-Isocitrate dehydrogenase
10% SDS-PAGE
antibody (ab184615)
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All lanes : Anti-Isocitrate dehydrogenase
antibody [GT1521] (ab184615) at 1/1000
dilution
Lane 1 : 293T whole cell lysate/extract
Lane 2 : A431 whole cell lysate/extract
Lane 3 : HeLa whole cell lysate/extract
Lane 4 : HepG2 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Western blot - Anti-Isocitrate dehydrogenase
antibody (ab184615)
Predicted band size : 46 kDa
10% SDS-PAGE
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
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