Product datasheet Anti-Lhx2 antibody [EPR9539] ab140614 1 Image Overview Product name Anti-Lhx2 antibody [EPR9539] Description Rabbit monoclonal [EPR9539] to Lhx2 Tested applications WB Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal) Positive control Daudi, HeLa, T.T. and HepG2 cell lysates General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Properties Form Liquid Storage instructions Shipped at 4°C. Store at -20ºC. Storage buffer Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR9539 Isotype IgG Applications Our Abpromise guarantee covers the use of ab140614 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa). 1 Application notes Is unsuitable for Flow Cyt,ICC,IHC-P or IP. Target Function Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types. Sequence similarities Contains 1 homeobox DNA-binding domain. Contains 2 LIM zinc-binding domains. Domain LIM domains are necessary for transcription activation. Cellular localization Nucleus. Anti-Lhx2 antibody [EPR9539] images All lanes : Anti-Lhx2 antibody [EPR9539] (ab140614) at 1/1000 dilution Lane 1 : Daudi cell lysate Lane 2 : HeLa cell lysate Lane 3 : T.T. cell lysate Lane 4 : HepG2 cell lysate Lysates/proteins at 10 µg per lane. Secondary Western blot - Anti-Lhx2 antibody (ab140614) HRP labelled goat anti-rabbit IgG at 1/2000 dilution Predicted band size : 44 kDa Observed band size : 45 kDa Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions 2 Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3