Product datasheet
Anti-Isocitrate dehydrogenase antibody [EPR12296]
ab172964
1 Abreviews 3 Images
Overview
Product name
Anti-Isocitrate dehydrogenase antibody [EPR12296]
Description
Rabbit monoclonal [EPR12296] to Isocitrate dehydrogenase
Tested applications
WB, IHC-P, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Cow, Orangutan
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human Isocitrate dehydrogenase aa 1-100 (Cysteine residue). The exact sequence is
proprietary.
Database link: O75874
Positive control
HepG2, HeLa, Raji and fetal kidney lysates; Human breast carcinoma tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR12296
Isotype
IgG
Applications
1
Our Abpromise guarantee covers the use of ab172964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with
IHC staining protocol.
IP
Application notes
1/10 - 1/100.
Is unsuitable for Flow Cyt.
Target
Anti-Isocitrate dehydrogenase antibody [EPR12296] images
All lanes : Anti-Isocitrate dehydrogenase
antibody [EPR12296] (ab172964) at 1/1000
dilution
Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : Raji lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-Isocitrate dehydrogenase
Secondary
[EPR12296] antibody (ab172964)
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 47 kDa
Immunohistochemical analysis of paraffinembedded Human breast carcinoma tissue
labeling Isocitrate dehydrogenase with
ab172964 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Isocitrate
dehydrogenase [EPR12296] antibody (ab172964)
2
Western blot analysis on immunoprecipitation
pellet from (1) HepG2 cell lysate or (2) 1X
PBS (negative control) using ab172964 and
HRP-conjugated anti-rabbit IgG preferentially
detecting the non-reduced form of rabbit IgG.
Immunoprecipitation - Anti-Isocitrate
dehydrogenase [EPR12296] antibody (ab172964)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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