Product datasheet
Anti-Lactate Dehydrogenase antibody [AF9D1] ab85325
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Overview
Product name
Anti-Lactate Dehydrogenase antibody [AF9D1]
Description
Mouse monoclonal [AF9D1] to Lactate Dehydrogenase
Tested applications
WB, ELISA
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
His tagged recombinant Human Lactate Dehydrogenase protein, purified from E.coli.
Positive control
HeLa, HepG2, 293T, SK-N-MC, RD, PC12 and NIH3T3 cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.03% Sodium Azide
Constituents: 50% Glycerol, 0.01% BSA, HEPES, 0.15M Sodium chloride
Purity
Ammonium Sulphate Precipitation
Clonality
Monoclonal
Clone number
AF9D1
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab85325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/2000. Detects a band of approximately 30 kDa.
ELISA
1/2000.
Target
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
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Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A
metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family.
Post-translational
modifications
ISGylated.
Cellular localization
Cytoplasm.
Anti-Lactate Dehydrogenase antibody [AF9D1] images
All lanes : Anti-Lactate Dehydrogenase
antibody [AF9D1] (ab85325) at 1/2000
dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SK-N-MC cell lysate
Western blot - Lactate Dehydrogenase antibody
Lane 5 : RD cell lysate
[AF9D1] (ab85325)
Lane 6 : PC12 cell lysate
Lane 7 : NIH3T3 cell lysate
Observed band size : 30 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Valid for 12 months from date of delivery
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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