Analysis Protocol Raw Data

Analysis Protocol
Raw Data
Plot Residuals.
Computer Based
Curve Fitting
Estimate Initial Rates
Manually. Estimate
the error in your
Carry out a Bootstrap analysis
based on residual sampling.
Use Error Propagation to
Compute Initial Rates:
Is there anther way to do this?
Plot v and S using Lineweaver-Burke
and Hanes to estimate Km and Vm from
the initial rate estimates. Make sure you
propagate the errors in v into the axes
transformations, eg 1/v. Compute:
Use a Matlab to do the linear regression
and estimate the standard errors on the
fitted parameters. Weight the fit using
the error bars.
Present a table of
estimates for Km
and Vm using the
different methods.