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Analysis Protocol Raw Data Plot Residuals. Computer Based Curve Fitting Estimate Initial Rates Manually. Estimate the error in your determination: Carry out a Bootstrap analysis based on residual sampling. Estimate: Use Error Propagation to Compute Initial Rates: Is there anther way to do this? Plot v and S using Lineweaver-Burke and Hanes to estimate Km and Vm from the initial rate estimates. Make sure you propagate the errors in v into the axes transformations, eg 1/v. Compute: Use a Matlab to do the linear regression and estimate the standard errors on the fitted parameters. Weight the fit using the error bars. Present a table of estimates for Km and Vm using the different methods.