Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
Anti-beta IV Tubulin antibody [6E3]
Mouse monoclonal [6E3] to beta IV Tubulin
WB, Flow Cyt
Reacts with: Human
Full length Human recombinant beta IV Tubulin protein produced in HEK293T cells
(NP_006078).
HEK293T cells transfected with beta IV Tubulin, Jurkat cells, HeLa cells
Properties
Form
Storage instructions
Storage buffer
Purity
Purification notes
Clonality
Clone number
Isotype
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS
Protein A purified
Purified from Mouse ascites fluids
Monoclonal
6E3
IgG2b
Applications
Our Abpromise guarantee covers the use of ab119254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
Flow Cyt
1/2000. Predicted molecular weight: 50 kDa.
1/100. ab170192 -Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
Function
Sequence similarities
Domain
Post-translational modifications
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Belongs to the tubulin family.
The highly acidic C-terminal region may bind cations such as calcium.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Cytoplasm > cytoskeleton.
Cellular localization
Anti-beta IV Tubulin antibody [6E3] images
All lanes : Anti-beta IV Tubulin antibody [6E3]
(ab119254) at 1/2000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY beta IV Tubulin
Lysates/proteins at 5 µg per lane.
Western blot - Anti-beta IV Tubulin antibody [6E3]
(ab119254)
Predicted band size : 50 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone
(RC203945)
Flow cytometric analysis of HEK293T cells transfected with either beta IV Tubulin overexpress plasmid (Red) or empty vector control plasmid (Blue), immunostained with ab119254 at a 1/100 dilution.
Flow Cytometry - Anti-beta IV Tubulin antibody
[6E3] (ab119254)
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Flow cytometric analysis of HeLa cells immunostained with ab119254 at a 1/100 dilution (Red) or a nonspecific negative control antibody,(Blue).
Flow Cytometry - Anti-beta IV Tubulin antibody
[6E3] (ab119254)
Flow cytometric analysis of Jurkat cells immunostained with ab119254 at a 1/100 dilution (red) or a nonspecific negative control antibody,(Blue).
Flow Cytometry - Anti-beta IV Tubulin antibody
[6E3] (ab119254)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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