Product datasheet Anti-TUBA1 + TUBA3 + TUBA4 antibody ab110386 1 Image Overview Product name Anti-TUBA1 + TUBA3 + TUBA4 antibody Description Rabbit polyclonal to TUBA1 + TUBA3 + TUBA4 Specificity ab110386 detects endogenous levels of total TUBA1 + TUBA3 + TUBA4 protein. Tested applications WB Species reactivity Reacts with: Rat Predicted to work with: Mouse, Human Immunogen A synthesized non-phosphopeptide derived from Human TUBA1 + TUBA3 + TUBA4 around the phosphorylation site of tyrosine 272 (A-T-YP-A-P). Positive control Rat brain cells lysate Properties Form Liquid Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride Purity Immunogen affinity purified Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab110386 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes 1/500 - 1/1000. Predicted molecular weight: 50 kDa. Target Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an 1 exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Tissue specificity Expressed at a high level in fetal brain. Involvement in disease Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem. Sequence similarities Belongs to the tubulin family. Post-translational modifications Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. Cellular localization Cytoplasm > cytoskeleton. Anti-TUBA1 + TUBA3 + TUBA4 antibody images All lanes : Anti-TUBA1 + TUBA3 + TUBA4 antibody (ab110386) at 1/500 dilution Lane 1 : Rat brain lysate Lane 2 : Rat brain lysate with blocking peptide at 10 µg Western blot - TUBA1 + TUBA3 + TUBA4 Lysates/proteins at 30 µg per lane. antibody (ab110386) Predicted band size : 50 kDa Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours 2 We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3