Product datasheet
Anti-beta I Tubulin antibody [SAP.4G5] ab11312
5 References 1 Image
Overview
Product name
Anti-beta I Tubulin antibody [SAP.4G5]
Description
Mouse monoclonal [SAP.4G5] to beta I Tubulin
Specificity
No reactivity with other tubulin isotypes is observed. The antibody is specific for beta I and does
not recognize beta II, III, or IV by western blot. Beta VI does contain the sequence. However,
there is no experimental proof that the antibody recognizes this isoform.
Tested applications
IHC-P, ELISA, ICC/IF, WB
Species reactivity
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis
Immunogen
Synthetic peptide corresponding to the C-terminal sequence of beta-tubulin isotype I, coupled to
BSA.
Epitope
Ab11312 specifically recognizes an epitope located in the C-terminal sequence of beta tubulin
isotype I of human, bovine, dog, hamster, rat, mouse, chicken and Xenopus.
Positive control
Cultured chicken fibroblasts.
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged
storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Purity
Ascites
Clonality
Monoclonal
Clone number
SAP.4G5
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab11312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
IHC-P
ELISA
ICC/IF
WB
Application notes
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution (methanolacetone and 3% paraformaldehyde-0.5%
Triton-x-100 fixations).
IHC-P: Use at an assay dependent dilution (10% neutral buffered formalin-alcoholic formalinxylene, paraffin-embedded).
WB: 1/20000, this dilution is determined using cultured chicken fibroblasts. Predicted molecular
weight: 55kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an
exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Involvement in disease
Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related
(MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased
platelet size and decreased number of circulating platelets.
Sequence similarities
Belongs to the tubulin family.
Post-translational
modifications
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs
exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl
group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in
human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and
flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the
mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and
lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of
such modifications is still unclear but they regulate the assembly and dynamics of axonemal
microtubules.
Cellular localization
Cytoplasm > cytoskeleton.
Anti-beta I Tubulin antibody [SAP.4G5] images
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ab11312 (1µg/ml) staining beta 1 tubulin in
human placenta using an automated system
(DAKO Autostainer Plus). Using this protocol
there is strong cytoplasmic staining.
Sections were rehydrated and antigen
retrieved with the Dako 3 in 1 AR buffer
EDTA pH 9.0 in a DAKO PT link. Slides were
peroxidase blocked in 3% H2O2 in methanol
for 10 mins. They were then blocked with
Immunohistochemistry (Formalin/PFA-fixed
Dako Protein block for 10 minutes (containing
paraffin-embedded sections)-beta I Tubulin
casein 0.25% in PBS) then incubated with
antibody [SAP.4G5](ab11312)
primary antibody for 20 min and detected with
Dako Envision Flex amplification kit for 30
minutes. Colorimetric detection was
completed with Diaminobenzidine for 5
minutes. Slides were counterstained with
Haematoxylin and coverslipped under
DePeX. Please note that, for manual staining,
optimization of primary antibody
concentration and incubation time is
recommended. Signal amplification may be
required.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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