Anti-Apg7 antibody [MM0968-2R37] ab211661 Product datasheet 1 Image Overview

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Product datasheet
Anti-Apg7 antibody [MM0968-2R37] ab211661
1 Image
Overview
Product name
Anti-Apg7 antibody [MM0968-2R37]
Description
Mouse monoclonal [MM0968-2R37] to Apg7
Tested applications
IHC-P, ICC, WB
Species reactivity
Reacts with: Mouse, Human
Immunogen
Recombinant fragment corresponding to Human Apg7 aa 387-570.
Database link: O95352
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Constituent: 100% PBS
Purity
Protein G purified
Purification notes
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.
Clonality
Monoclonal
Clone number
MM0968-2R37
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab211661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/100 - 1/200.
ICC
1/100 - 1/200.
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
Target
1
Function
Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and
ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1,
GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy.
Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3.
Tissue specificity
Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Sequence similarities
Belongs to the ATG7 family.
Domain
The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and
ATG12.
Cellular localization
Cytoplasm.
Anti-Apg7 antibody [MM0968-2R37] images
Anti-Apg7 antibody [MM0968-2R37]
(ab211661) at 1/1000 dilution + HeLa cell
lysate
Predicted band size : 78 kDa
Western blot - Anti-Apg7 antibody [MM09682R37]
(ab211661)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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