Product datasheet Anti-Apg7 antibody [MM0968-2R37] ab211661 1 Image Overview Product name Anti-Apg7 antibody [MM0968-2R37] Description Mouse monoclonal [MM0968-2R37] to Apg7 Tested applications IHC-P, ICC, WB Species reactivity Reacts with: Mouse, Human Immunogen Recombinant fragment corresponding to Human Apg7 aa 387-570. Database link: O95352 Positive control HeLa cell lysate. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Storage buffer Constituent: 100% PBS Purity Protein G purified Purification notes The IgG fraction of culture supernatant was purified by Protein G affinity chromatography. Clonality Monoclonal Clone number MM0968-2R37 Isotype IgG1 Applications Our Abpromise guarantee covers the use of ab211661 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes IHC-P 1/100 - 1/200. ICC 1/100 - 1/200. WB 1/1000 - 1/2000. Predicted molecular weight: 78 kDa. Target 1 Function Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Tissue specificity Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Sequence similarities Belongs to the ATG7 family. Domain The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. Cellular localization Cytoplasm. Anti-Apg7 antibody [MM0968-2R37] images Anti-Apg7 antibody [MM0968-2R37] (ab211661) at 1/1000 dilution + HeLa cell lysate Predicted band size : 78 kDa Western blot - Anti-Apg7 antibody [MM09682R37] (ab211661) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2