Product datasheet
Anti-KRT23 antibody [AT2F6] ab139362
2 Images
Overview
Product name
Anti-KRT23 antibody [AT2F6]
Description
Mouse monoclonal [AT2F6] to KRT23
Tested applications
Flow Cyt, WB
Species reactivity
Reacts with: Human
Immunogen
Recombinant fragment, corresponding to amino acids 271-422 of Human KRT23 purified from
E. coli.
Run BLAST with
Run BLAST with
Positive control
HeLa cell lysate
General notes
ab139362 is derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant Human KRT23 protein.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Purity
Protein G purified
Clonality
Monoclonal
Clone number
AT2F6
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab139362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Flow Cyt
Use 2-5µg for 106 cells.
WB
1/500 - 1/3000. Predicted molecular weight: 48 kDa.
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Target
Sequence similarities
Belongs to the intermediate filament family.
Anti-KRT23 antibody [AT2F6] images
Anti-KRT23 antibody [AT2F6] (ab139362) at
1/3000 dilution + HeLa cell lysate at 30 µg
Secondary
Goat anti-mouse secondary antibody
conjugated to HRP
developed using the ECL technique
Predicted band size : 48 kDa
Western blot - Anti-KRT23 antibody [AT2F6]
(ab139362)
Flow cytometry analysis of HeLa cells
labelling KRT23 with ab139362 at 2-5 µg for
1x106 cells. The secondary antibody used
was Alexa Fluor® 488 goat-anti-mouse IgG.
Flow Cytometry - Anti-KRT23 antibody [AT2F6]
(ab139362)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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