Product datasheet Anti-IST1 antibody [22] ab87456 1 References 1 Image Overview Product name Anti-IST1 antibody [22] Description Mouse monoclonal [22] to IST1 Tested applications WB, ELISA Species reactivity Reacts with: Human Immunogen Recombinant full length protein expressed in E. Coli Positive control H520 whole cell lysate Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Storage buffer Preservative: 0.02% Proclin Constituents: Citrate-Tris HCl, pH 7.0 Purity Protein G purified Clonality Monoclonal Clone number 22 Isotype IgG1 Applications Our Abpromise guarantee covers the use of ab87456 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes WB 1/1000. Predicted molecular weight: 40 kDa. ELISA Use at an assay dependent dilution. Target Function Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvment in the MVB pathway is 1 not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. Sequence similarities Belongs to the IST1 family. Cellular localization Cytoplasmic vesicle. Localizes to the midbody of dividing cells. Anti-IST1 antibody [22] images Anti-IST1 antibody [22] (ab87456) at 1/1000 dilution + H520 whole cell lysate Predicted band size : 40 kDa Western blot - IST1 antibody [22] (ab87456) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2