Product datasheet Anti-Apg10 antibody [1F3-C9-D7] ab130142 1 Image Overview Product name Anti-Apg10 antibody [1F3-C9-D7] Description Mouse monoclonal [1F3-C9-D7] to Apg10 Tested applications WB Species reactivity Reacts with: Recombinant Fragment Predicted to work with: Human Immunogen Recombinant fragment from the N-terminus of Human Apg10 (Q9HOY0). Positive control Recombinant Apg10. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. Storage buffer pH: 7.40 Preservative: 0.02% Sodium azide Constituents: 48% PBS, 50% Glycerol, 1% BSA Purity Protein G purified Clonality Monoclonal Clone number 1F3-C9-D7 Isotype IgG Applications Our Abpromise guarantee covers the use of ab130142 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes 1/100 - 1/2000. Predicted molecular weight: 25 kDa. Target Function E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an 1 ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Sequence similarities Belongs to the ATG10 family. Cellular localization Cytoplasm. Anti-Apg10 antibody [1F3-C9-D7] images Anti-Apg10 antibody [1F3-C9-D7] (ab130142) at 1/2000 dilution + recombinant Apg10 protein with a DsbA tag Predicted band size : 25 kDa Western blot - Anti-Apg10 antibody [not given] (ab130142) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2