UCMR3 537 (Perfluorinated Compounds) Sampling Instructions
1. a) FREEZE GELPAKS UPON RECEIPT OF SAMPLE KIT AND ADD FROZEN GELPAKS TO THE COOLER ON THE
DAY OF SAMPLING.
b) WHEN SAMPLING, BRING OTHER AVAILABLE BAGGED WET ICE IN SEALED BAGS OR FROZEN GELPAKS
TO CHILL SAMPLES DURING SAMPLE COLLECTION.
2. The sampler will receive the following sample kit from our lab:
Bottle Label
@UCMR3 537
@UCMR3 537
TB
@UCMR3 537
FB
# of
Bottles
3
Size
Bottle
Preservative
275mL
Polypropylene (PP) bottles with PP
screw-caps (no round label on bottle)
PP bottles with PP screw-caps (white
round label on bottle)
PP bottles with PP screw-caps (blue
round label on bottle)
1.4g of Trizma
1
275mL
1
275mL
1.4g of Trizma
None
* The sampler will receive the Trip Blank (TB) filled with water and preservative. It is colored in the picture to indicate
the bottle is filled (not empty) for picture purposes only. **The sampler will also receive an empty bottle labeled
@UCMR3 537 FB (Field Blank) and 3 sets of preserved bottles.
3. Put on nitrile gloves. While at the site, before collecting samples, open the @UCMR3 537
TB labeled bottle containing the preserved reagent water.
4. Pour the preserved reagent water into the empty bottle labeled (@UCMR3 537 FB)
Cap both the filled @UCMR3 537 FB and the now empty TB bottle. Ship the filled FB and
the now empty @UCMR3 537 TB bottle back to the lab along with the samples.
5. If sampling from faucet, remove the aerator and screen.
6. Open the tap and let the water of the sample source run at fast flow for approximately 5
minutes.
7. Use indelible ink (pen included in kit) to clearly identify the sample bottles with the
information listed below.
-Sample ID
-Source of sample, if not already on label
-Analysis required, if not already on label
-Date and Time of collection
-Preservative used, if not already on label
UCMR3 537 Page 1 of 2
Revised: 11/14/12
8. Slow water flow to thickness of a pencil (to minimize splashing) and fill bottle.
9. Fill sample bottle up to bottom of neck, taking care not to flush out preservatives and
making sure the mouth of the bottle does not come in contact with anything other than
sample water.
10. Cap and invert the bottles at least 5 times to mix the sample with the preservative.
11. Collect sample for the other 2 sample bottles by repeating steps 8 to 10.
SHIPPING SAMPLES AND STORAGE
1.
2.
3.
If shipping samples on the same day of sampling, chill samples until at or below 10°C by exchanging the ice used
during sampling with available sealed bag of fresh frozen ice or frozen gelpaks.
Pack chilled samples in a cooler with FROZEN gelpaks.
Complete the Chain of Custody during sample collection. Place completed Kit Order and completed Chain of Custody
in a ziplock bag in the cooler on top of packing material. The following information is required on the completed Chain
of Custody.
- Collector’s name
-Unique field sample ID (from UCMR database)
-PWSID #
-Facility ID # (from UCMR database)
4.
5.
6.
7.
8.
9.
-Date and time of collection
-Comments about the sample, if applicable
-Sample type (EP, MR, or FB)
-Sample Event Number (SE1, SE2, SE3, SE4)
Ship via overnight service such as FEDEX, UPS, or DHL, etc. Sample must not exceed 10˚C during transit.
Samples MUST arrive at lab within 48 hours of sampling at or less than 10˚C, greater than 0˚C (not frozen).
If samples are received more than 48 hours after sampling they must be at or less than 6˚C, greater than 0˚C
(not frozen).
If samples are received on the same day as collection, temperature may be greater than 10˚C with evidence of cooling
such as frozen gelpaks or wet ice.
Maximum HOLDING TIME FOR SAMPLES IS 14 DAYS from time of collection. Sample extracts can be held for a
maximum of 28 days.
Alternatively, cool the samples down by placing them overnight in a cooler with frozen refrigerant packs or water ice,
or in a refrigerator (store chilled for at least 12 hours before packing for shipment). Maintain the samples cold until
repacked in the cooler for shipment to the lab
ADDITIONAL NOTES
1.
Do not composite (i.e., combine, mix or blend) UCMR3 samples.
2.
Avoid handling potential contamination such as food packaging and certain foods and beverages before sample collection. Wash
hands before sampling and wear powderless nitrile gloves (included in kit) while filling and sealing the sample bottles.
3.
Collect samples early enough in the day to allow adequate time to cool and to send those samples for overnight delivery to the
laboratory, if not refrigerated and stored overnight before shipping.
Try to collect only on a Monday, Tuesday or Wednesday and ship no later than Thursday of each week, and try to NOT collect
4.
samples on Friday, Saturday, or Sunday unless special arrangements have been made for the receipt of samples at the laboratory
within 48-hours of collection.
5.
If in doubt, please review our YouTube sampling video at http://www.youtube.com/user/EurofinsEaton.
UCMR3 537 Page 2 of 2
Revised: 11/14/12