HORSE FALL 2005 H E A LT H L I N E S B R I N G I N G B E T T E R H E A LT H TO YO U R H O R S E S BLINDED BY THE NIGHT Do genetics connect coat patterns and night blindness in Appaloosa horses? WESTERN COLLEGE OF VETERINARY MEDICINE • EQUINE HEALTH RESEARCH FUND It’s tough enough planning your own future — try imagining the future of the large animal surgery area at the Western College of Veterinary Medicine. By 2025, what will horse owners expect in terms of surgical facilities and resources? What knowledge and skills will future veterinary students require? I N S I D E 2 The Cutting Edge in Equine Surgery 4 Blinded by the Night 8 A Region’s Colic Profile 9 Ultrasound Exposé Advanced technology and a new floor plan give WCVM’s large animal surgery area an edge. Is congenital stationary night blindness associated with certain coat patterns in Appaloosa horses? A WCVM study gives a western Canadian perspective to a common equine disease. Ultrasonography may give veterinarians a quicker diagnosis of deadly colic called large colon volvulus. 10 Front Line Support Fred Clement’s contributions to equine health span three decades and generations of western Canadian veterinarians. 12 WCVM Case Report New imaging technology helps clinicians measure the damage caused after a common fungus ends up in an uncommon place. FRONT COVER: A horse’s retinal fundus or the interior lining of the eyeball. The pink oval is the eye’s optic nerve while the green area is the reflective tapetum (reflecting layer) that’s responsible for the “eyeshine” seen when light hits the eye. Photo: Dr. Lynne Sandmeyer. H O R S E H E A LT H L I N E S Horse Health Lines is produced by the Western College of Veterinary Medicine’s Equine Health Research Fund. Visit www.ehrf.usask.ca for more information. Please send comments to: Dr. Hugh Townsend, Editor, Horse Health Lines WCVM, University of Saskatchewan 52 Campus Drive, Saskatoon, SK S7N 5B4 Tel: 306-966-7453 • Fax: 306-966-7274 wcvm.research@usask.ca For article reprint information, please contact sm.ridley@sasktel.net. 2 Horse Health Lines • Fall 2005 Those questions delve into familiar territory for Dr. Bruce Grahn, who has chaired two phases of the WCVM Veterinary Teaching Hospital expansion committee since 2000. He and his committee members have done plenty of speculating about what types of diseases and health issues that veterinarians may eventually face. But as the veterinary ophthalmologist points out, listening to people and monitoring the demands of today give some of the best clues to understanding future animal health trends. “If we did anything well, I think it was the fact that we spent a lot of time listening to people — staff, faculty, clients, students, administrators — everyone,” says Grahn. “We started with basic questions: what do you need? What do you want? Then we repeated that process over and over again. It’s always challenging for people to talk about modifying where they work and how they work. But as they had time to think about it, people became more open to making changes for the overall benefit of this hospital.” Grahn says it soon became obvious where the committee needed to allocate space and dollars in the hospital’s Large Animal Clinic. For example, one necessity was a safer chute complex for large animals. Another essential was a larger surgery area with more centralized services. How much of a problem is space in large animal surgery? “Inside the suite where we do orthopedic surgeries, room is very tight when you have an anesthetized horse on the table,” describes Dr. David Wilson, another expansion committee member and head of the Large Animal Clinical Sciences department. “We can do the surgeries because most of the action takes place around one limb, but it’s difficult to squeeze around the rest of the patient — and that can be a big problem for our support staff who need to move around the entire room.” Wilson adds that space isn’t the only problem: surgical suites are cluttered with medical equipment and supplies, the floors often become a maze of electrical cords and hoses during complicated procedures, and 30-year-old surgical lights are showing their age. The new renovation plans address all of these concerns, plus they create a “closed surgery” where people must change into surgical attire before entering and re-entering the area. “This will reduce the chances of bringing infection into the area as well as taking infection out to other parts of the hospital,” explains Wilson. New features — such as separate rooms for anesthesia-induction and recovery — will also help to minimize contamination if a potential risk arises, adds Wilson. “If we find an internal abscess in a horse during colic surgery, then we know the only places where the infection is present is in the surgical suite and the recovery room. With the new floor plan, we can move that horse into an isolation unit on the outside of the building and prevent contamination of other areas.” The expansion is also ushering in new technology: the hospital added computed tomography (CT) to its medical imaging department in 2004, and nuclear scintigraphy will soon become the College’s newest diagnostic tool. “There’s no question that advanced diagnostics will become much more critical in veterinary teaching hospitals,” stresses Grahn. “If you’re not equipped for the future, you’ll fall behind.” CHUTE COMPLEX 5 6 MEDICAL IMAGING BOVINE WARD BOVINE SURGERY CALF ISOLATION UNIT 1 3 LOCKERS NURSES’ STATION 1 1 4 EQUINE SURGERY EQUINE SURGERY 4 RECOVERY INDUCTION ROOM EQUINE ISOLATION UNITS EQUINE WARD 2 RECOVERY ROOM 4 4 The CUTTING EDGE By upgrading the hospital’s surgical facilities and introducing more advanced technology, WCVM will be capable of expanding its large animal caseload. While that’s important for undergraduate veterinary education, Grahn says it’s especially critical to the quality of the College’s graduate training program — Western Canada’s source for future specialists, faculty members and researchers. Will this be the large animal surgery of everyone’s dreams? Probably not, says Grahn with a soft chuckle. The hospital expansion committee does have to work within space and budgetary limits. But for Grahn and his colleagues, 1. Surgery • What’s new? The closed surgery includes two equine surgical suites, plus one suite for standing surgeries in cattle. All suites have wide doors so the CT scanner, the C-arm fluoroscope or other diagnostic equipment can be wheeled in and out. Suites cluster around a centralized nursing and medical supply area with ready access to anesthesia-induction areas and to medical imaging. Each suite has its own recovery room close to anesthesia and to the equine ward. • Other features? Each suite has new surgical lights that are “camera capable” for digital imagery. Flat panel LCD monitors hang over the operating tables so specialists can view laparascopic images or review digital X-ray images. Anesthetic “booms,” medical gases, suction and electrical cords drop down from the ceiling to minimize clutter. 2. Anesthesia • What’s new? The anesthesia area gains a separate room for induction so pre-surgery patients are separated from post-surgery patients. Anesthesiologists have overhead access to medical gases in each surgery suite, plus ventilators and other critical care equipment can be easily wheeled in. 3. Medical Imaging • What’s new? A CT scanner can be wheeled in to both equine surgery suites, plus equine patients can be led to the new nuclear scintigraphy room located a few metres away from the equine ward. in Equine Surgery what’s more important than extra “bells and whistles” is the fact that the final layout will provide a high-quality training ground for new generations of veterinarians. Plus, it will be a place where people can perform to the best of their abilities — no matter what new health challenge arises in the next 20 years. H How can I make a donation to the VTH Expansion Campaign? • Use the enclosed tear out card • Visit www.wcvm.com/supportus • Contact WCVM development officer Joanne Wurmlinger at 306-966-7450 (joanne.wurmlinger@usask.ca). 4. Equine Ward and Isolation Units • What’s new? Barriers separate the equine and bovine wards, plus both wards can be closed off from the rest of the hospital during a biosecurity risk. Three equine isolation units have separate anterooms where staff change clothing before and after entering the units. As in the past, hospital staff will admit infectious equine patients into isolation units from outside doors to avoid contact with other patients in the equine ward. Other Points of Interest 5. Large animal chute complex allows hospital staff to move animals into one of three separate work areas or to return patients to their trailers. It also provides ready access to the bovine standing surgical suite, to medical imaging and to the bovine ward. 6. Calf isolation unit has an outside entrance, 10 stalls and a separate anteroom where staff change their clothing before and after entering the unit. Western College of Ve t e r i n a r y M e d i c i n e 3 T HE HORSES ARE IN TROUBLE. That’s all Sheila Archer could think when she awoke with a start at 2 a.m. during an early spring storm in 2003. As rain pelted against the windows of their farmhouse south of Moose Jaw, Sask., Archer and her husband hurriedly dressed and headed outside into the pitch-black night. Their four purebred Appaloosa mares should have been standing on the well-protected, south side of the barn — but not one horse was there. “We went out to our north pasture, and there they were: standing on a hill, shivering and hypothermic in the cold rain,” recalls Archer. What stood between the horses and shelter from the storm was an S-curved, dirt roadway that crossed over a culvert draining the farm’s dugout. When the couple moved their mares to the farm months earlier, they had run electric fence on either side of the wide causeway — a precaution that posed no problems for the horses in the daylight. “But at night and in the middle of that storm, they would not cross the causeway to get to the barn. They finally crossed but only after we talked to them, put on their halters and led them through to the other side,” says Archer. “And that’s when I knew my horses couldn’t see in the dark.” Nearly three years after that memorable night, Archer now knows with certainty what causes her horses’ vision to disappear after sundown. First described in the late 1970s, congenital stationary night blindness (CSNB) is thought to be a hereditary, non-progressive condition found in some horses. All reported cases have involved Appaloosa horses so far, but because little is known about the disease, it may also affect horses of other breeds. Other than Dr. D.A. Witzel’s initial CSNB research in Appaloosa horses nearly three decades ago, very little information about the disease is available in veterinary literature. In fact, one of the “bibles” of veterinary ophthalmology textbooks contains only one paragraph describing the disease, says Dr. Lynne Sandmeyer, a veterinary ophthalmologist at the University of Saskatchewan’s Western College of Veterinary Medicine (WCVM). Few words are written about CSNB in horses — but Sandmeyer thinks the condition may occur more often than horse owners and veterinarians think. Since most owners don’t have much interaction with their horses after dark, the condition’s symptoms — such as a horse showing some anxiety in darkness or closely following another pasture mate at night — can go unnoticed. “Under normal circumstances, affected horses can cope with the condition because they’re born with it — it’s not like something suddenly changes in their lives. They just think that’s the way the world works: at night, the lights go out and they can’t see,” explains Sandmeyer, who is leading one of the most comprehensive research investigations of CSNB in Appaloosa horses ever undertaken. Blinded by the NIGHT Project sheds light on eye disease For the next two years, the College’s Equine Health Research Fund is providing financial support for Sandmeyer’s research team whose members include veterinary ophthalmologist Dr. Bruce Grahn and Dr. Carrie Breaux, a veterinary ophthalmology resident. In the first phase of the study, the research team will conduct detailed eye examinations on 30 Appaloosa horses as well as on 10 Arabian horses (the study’s control group). Since scientists suspect that CSNB is caused by a problem during the process of transmitting information from a horse’s eyes to its brain, the project includes a detailed series of electroretinographic (ERG) testing on each horse. “The ERG testing will confirm the disease in horses, plus it will also allow us to study the exact electrical responses and to see if we can pinpoint where the transmission problem occurs,” says Sandmeyer. “We’ll examine the horses’ eyes to check for any abnormalities, and we’ll test whether any of the animals are nearsighted or farsighted since anecdotal reports have suggested that CSNB-affected horses are nearsighted.” The research team will conduct more detailed anatomical studies of affected eyes, then do further immunohistochemistry testing to try and pinpoint any physiological abnormalities. “We’ll also be collecting blood from the horses so at some point, we can investigate the gene for the disease,” adds Sandmeyer, who says the ultimate goal is to develop gene therapy for CSNB. “If nothing is morphologically wrong with the horse’s retinal cells, we may eventually be able to provide those cells with the material that they need to activate the physiological L O S T i n Tr a n s l a t i o n Look deep into the eyes of a horse affected with congenital stationary night blindness (CSNB) and you’ll see nothing but liquid pools of colour and light. Even specialists like WCVM’s Dr. Lynne Sandmeyer — equipped with the latest in ophthalmic technology — haven’t discovered anything unusual about the retinas of an animal affected with the condition. That’s because the actual problem that causes this type of blindness is virtually invisible — occurring somewhere during the process of transmitting information from the eyes to the horse’s brain. As Sandmeyer explains, cells called photoreceptors are responsible for transmitting information from the retina to the brain. Cone-shaped photoreceptors take care of colour and day vision, while rod-shaped photoreceptors are responsible for vision in the dark. Normally, rod photoreceptors pick up the light that comes in the eye and transmits it into an electrical response. With the release of neurotransmitting chemicals, the rod photoreceptors relay the electrical response through other processing cells until it eventually reaches the brain where the information is transformed into an image. But in a horse diagnosed with CSNB, something stops this neurological “Pony Express” from happening normally: “We know the rods are there, and they’re not abnormally shaped — but somewhere along the line, the information is dropped or not transmitted properly,” says Sandmeyer. Those transmission problems are mirrored in electroretinograms that are used to make a definite diagnosis of CSNB. After keeping a horse in the dark for at least 20 minutes, a veterinary ophthalmologist conducts a series of tests using electroretinography (ERG) to pick up the electrical information of photoreceptors and other cells in the horse’s retina as they respond to light. “A normal ERG consists of two waves showing the responses of the photoreceptors. The A-wave has a downward peak that goes down a little bit, and then there’s usually a slower, upward peak that’s called the B-wave,” explains Sandmeyer. “In a horse with CSNB, that first A-wave shows up, but instead of a B-wave showing up, the A-wave just continues to go down. In other words, the information is dropped so there’s no image.” Will scientists ever “see” what causes CSNB to happen? The WCVM-led investigation of the disease’s characteristics may uncover some clues, but Sandmeyer suspects it will be a long process. “One of the next steps we can take is to look at the physiological differences between the eyes of normal horses and the eyes of horses diagnosed with CSNB. With the use of immunohistochemistry, we could try and pin down whether it’s the transmitters, the transmitter receptors or other aspects of the retina that aren’t working.” She adds that this could be another way for genetic researchers to identify the gene responsible for the disease and its location in the horse’s genetic makeup: “If we find out that CSNB occurs because a certain neurotransmitter is not there, then we would know that the genetics involved in developing that neurotransmitter is the source of the problem. It’s simply taking a different approach to finding the gene responsible for CSNB.” Solving the CSNB puzzle in horses is valuable to veterinary medicine, but the information may also help researchers gain a better understanding of the equivalent disease in humans. As Sandmeyer points out, the ERG abnormalities that show up in the eyes of CSNB-affected horses are similar to the results recorded in humans affected by the Schubert-Bornshein type of CSNB. “If we identify the CSNB gene in horses, it could potentially benefit the work of researchers involved in human CSNB studies by narrowing down their options. Just as we use human research, they could test whether our gene is causing the same disease in humans.” H process and allow them to work at night,” explains the researcher. “That’s many years down the road, but someday, we may have the means to help these horses.” in the Appaloosa breed — a hypothesis based on anecdotal reports from horse owners and on results from genetic studies conducted by the Appaloosa Project’s researchers. Last fall, someone who has personal experience with CSNB — Sheila Archer — presented that hypothesis continued The Appaloosa Project link The WCVM study is also part of a larger, genetic research initiative called the Appaloosa Project whose research collaborators are striving to identify and isolate the main genes responsible for Appaloosa patterning, and to investigate key physical traits associated with these genes. For instance, one possibility is that the occurrence of CSNB is associated with a certain type of coat pattern found “This is a manageable condition, and if owners know their horses are affected by this disease, they can put measures into place that keep their horses — and their families — safe.” to WCVM’s team of veterinary ophthalmologists. Besides being an Appaloosa breeder, the phenotype (physical traits-based) researcher co-ordinates the North America-wide Appaloosa Project from her home near the small rural community of Spring Valley, Sask. In 2003, Archer was involved in a genome scan conducted by Dr. Rebecca Bellone, a genetic researcher from the University of Tampa and one of the Appaloosa Project’s collaborators. That scan mapped the location of a gene called leopard complex or Lp — the gene responsible for “turning on” the Appaloosa spotting pattern — to a region on equine chromosome 1. When geneticists have isolated a gene to a section of a chromosome, they still need to identify the actual gene within the region that’s responsible for the trait they’re studying. One method used to find the target gene is the “candidate gene” approach. “Since scientists have extensively mapped the genomes of the human and the mouse, we compared the region of equine chromosome 1 that PRECEDING PAGES: Owyhee’s Eve (left), an Appaloosa mare owned by Sheila Archer, has CSNB. Top, circle: Archer’s mare, Tyee’s Blue Belle, whose coat pattern is mid-way between the snowcap and fewspot patterns. Belle has CSNB. Bottom right: Ninita, owned by Archer, is an Appaloosa yearling filly with a near-leopard coat pattern. Ninita doesn’t have CSNB. Photos: Sheila Archer. A Project Lp resides in to matching sections of human and mouse chromosomes, and we found some obvious suspects involved in pigmentation,” explains Archer. One candidate gene for Lp is oculocutaneous albinism type 2 (OCA2) that’s associated with very low levels of pigmentation and night blindness in humans. In mice, the most common form of this gene is associated with a totally white appearance and pink eyes. But scientists have identified many different identified versions of the gene — some of which cause a mottled, roaned coat pattern. After Bellone identified OCA2 as a candidate for the Lp gene, Archer began investigating phenotype-based information about Appaloosas that would help to confirm whether or not Lp is a mutation at the same locus or “genetic address” in the horse as OCA2 is in humans. During her research, she collected stories from owners whose Appaloosa horses had shown signs of blindness in the darkness or dim light. In most cases, affected horses were “few spot” or “snowcap” Appaloosas — the breed’s descriptions for relatively spot-free coat patterns. These same patterns occur on horses that have been confirmed as homozygous (having two identical genes at the corresponding position of similar chromosomes) for the Lp gene. Those stories intrigued Archer because the symptoms were similar to her own experiences with her horses’ vision. The coat pattern descriptions also fit her Appaloosa mares — all of which have few spot or snowcap patterning. Curious, she brought two of her horses to Dr. Bruce Grahn at WCVM’s Veterinary Teaching Hospital for eye examinations. She also brought along her suggestion that CSNB in Appaloosas may be associated with certain coat patterns — a possibility that became more plausible once Grahn confirmed that Archer’s horses had CSNB. “After Dr. Grahn finished examining my second horse, he said, ‘I think we’ve got something here,’ and I remember feeling really excited about what this could mean for the Appaloosa Project — that maybe we had our gene. At the same time, I felt strange. I was convinced that my horses had CSNB before I even came to Saskatoon, but as I stood there supporting my very drugged horse, it still upset me to know with certainty that she couldn’t see at night.” But rather than hiding that fact, Archer believes she and other Appaloosa breeders and owners need to know more about the disease to protect their of aDIFFERENT COLOUR There’s something truly unique about the Appaloosa Project — a North America-wide research initiative that focuses on the genetic nature of the Appaloosa breed. For instance, how many other scientists are linked to more than 700 horse owners through an on line classroom? How many other projects sell T-shirts so researchers can purchase PCR (polymerase chain reaction) test kits? And how many other genetic research projects can trace their creative spark to a 1966 Walt Disney movie called “Run Appaloosa, Run”? “I still get choked up thinking about that movie,” says Sheila Archer, who laughs as she readily gives Disney partial credit for her lifelong love of Appaloosa horses. That early fascination eventually led to Archer’s career as a phenotype (physical traits-based) researcher and to her pivotal meeting with another Appaloosa enthusiast five years ago. Back then, Dr. Rebecca Terry (now Bellone) was a PhD student at the University of Kentucky who was conducting research to locate the Lp (leopard complex) gene in the Appaloosa breed. When Bellone and Archer contacted the same Appaloosa breeder, the woman passed on Bellone’s email address to Archer. After corresponding for several months, the two eventually decided to pool together their resources. That partnership led to more collaborations with other genetic researchers at the Universities of Kentucky and Tampa. In 2003, the team of researchers made an important discovery: through a genome scan, they located the Lp gene — the gene responsible for “turning on” the Appaloosa spotting pattern — in a small region on equine chromosome 1. Soon after, the group formalized their research efforts as the Appaloosa Project. Besides Archer and Bellone, the project’s members include Drs. Ernest Bailey, Teri Lear, Gus Cothran and Ms. Samantha Brooks of the University of Kentucky, and Dr. David Adelson at Texas A & M University. Its newest members are Drs. Lynne Sandmeyer, horses as well as the people who work and interact with CSNB-affected animals. “It’s a lot easier on the horse if people have a better idea of what’s going on, so yes, I would love veterinarians, breeders and owners to know more about the condition. Then we can avoid potentially dangerous situations, we can learn how to better manage these horses and we can have a better understanding of how this condition may affect their behaviour and reactions to everyday things.” “CSNB-affected horses are healthy, viable and useful horses,” adds Sandmeyer. “This is a manageable condition, and if owners know their horses are affected by this disease, they can put measures into place that keep their horses — and their families — safe.” Spots and CSNB: association? To test whether CSNB is associated with the gene responsible for the Appaloosa spotting pattern, Archer is helping the WCVM research team track down 30 purebred horses — all with different pedigrees. Researchers will select horses that display three different types of coat patterns: few spot or snowcap (patterns with few or no spots), spotted leopard and spotted blanket patterns (plentiful spotting) and ‘true solid” Appaloosas, showing no coat pattern or any signs that Lp is present (horses that have Appaloosa parents but look like normally pigmented horses). Besides determining if there really is an association, the study will also answer researchers’ questions about whether there are variations in the severity of the disease among horses or changes in affected horses’ day vision. If all 10 of the project’s few spot- or snow cap-patterned horses are night blind, there are two possible explanations. It could mean that the Lp gene is also the causative mutation for CSNB in the breed. Or, another possibility is that the Lp gene lies extremely close to the gene containing the causative mutation for CSNB — so close that the two genes are linked. Either way, developing a test for Lp would be equivalent to developing a test for CSNB in Appaloosas, points out Archer. She adds that if the WCVM research team finds a direct correlation between CSNB and few spot and snowcap patterning in Appaloosas, it could save the Appaloosa Project’s researchers time in the hunt to discover the identity of the Lp gene. A better understanding of the condition’s genetic makeup would also help veterinary ophthalmologists like Sandmeyer eventually develop genetic therapy for CSNB. While researchers wrestle with this puzzling disease, Archer’s Appaloosa horses continue to graze, sleep and play in a world they can’t see after dusk. But some changes have occurred since that rainy night in 2003: the horses now spend their nights in a dimly lit barn and they’ve all foaled babies inside. Archer also brings her CSNB-affected horses in during thunderstorms since sudden flashes of lightning seem to disorient them. “Sure, there are some management issues, but it’s really not that hard. The only differences in my life is that I have to clean the barn every morning, and I pay a larger electrical bill for my barn,” says Archer. “But for me, I don’t think it’s a very big price to pay. A few chores and a little bump on my electrical bill are worth it if I can have these wonderful creatures in my life.” H FAR LEFT: Tyee’s Blue Belle. Above: The black snowcap patterned Owyhee’s Eve is one of 10 homozygous Lp-patterned horses participating in the WCVM study. Photos: Sheila Archer. Bruce Grahn and Carrie Breaux of the Western College of Veterinary Medicine. “This is a grassroots, volunteer initiative,” says Archer, who operates the on line classroom for horse enthusiasts around the world. Since the Appaloosa Project’s research activities involve a large number of horses, the discussion group has been an invaluable resource when Archer needs to track down Appaloosas with certain traits or to collect anecdotal evidence. “That’s a big difference between our group and other research groups: we’ve worked hard to keep those lines of communication open to horse owners and to help people understand what this project is all about.” “Sheila is amazing because she’s extremely knowledgeable about the breed, its genetic origins and the phenotype research,” adds Sandmeyer, who is working with Archer on the investigation of congenital stationary night blindness (CSNB). “Because she has built such a great network of Appaloosa breeders, she’s also been very helpful in getting access to horses that we can include in the study — which can be very difficult. This just wouldn’t happen without her.” Project funding comes from various sources: donations from Appaloosa breeders, equipment and material donations from universities, research grants and of course — proceeds from T-shirt sales. A new partner is WCVM’s Equine Health Research Fund that’s providing financial support for the two-year CSNB study. Another crucial partner is the Appaloosa Horse Club of Canada. The national breed organization has given the researchers access to registry records, funding and opportunities to communicate with its members. But the project’s largest donors are the researchers themselves who have contributed thousands of volunteer hours to the initiative. Their attraction to Appaloosas may not stem back to Walt Disney — but something about the breed has compelled them to be part of the project. “I think you could safely say that all of us think Appaloosas and their unique coat patterns are beautiful,” says Archer. “And because we’re so fascinated with the reasons why they look like that, we just have to work on this project. There’s no question.” A re draft horse breeds more prone to colic caused by large colon displacement? Are broodmares more prone to a colic caused by large colon volvulus (twisting of the large colon)? Does the gender of a horse make it more prone to developing colic caused by benign tumours called lipomas? Not necessarily at the Western College of Veterinary Medicine, says Dr. Sameeh Abutarbush. The veterinary internal medical specialist 2 most common causes among medical colic cases: large colon impaction and spasmodic colic.* is the main investigator in a new WCVM study that has developed the first comprehensive “colic profile” with a western Canadian angle to it. Instead of reflecting some of the predisposing factors identified in other colic investigations, the 10-year retrospective study of more than 600 colic cases presented at WCVM has recognized some new ones that are distinct to the region. For example, the research showed that geldings in the region are more prone to colic than mares and stallions and that a significant number of older horses (15 years and over) have suffered from colic caused by benign tumours called strangulating lipomas. “A great deal has been written about the causes of colic and its risk factors, but as we found, what’s reported in other regions and published in veterinary literature doesn’t necessarily reflect what we mainly deal with in Western Canada,” explains Abutarbush, who initiated the study in 2002 while completing his residency at WCVM. Abutarbush — with help from fellow A Region’s COLIC PROFILE 18 hours: the average time that colic survivors showed clinical signs before hospital admission (time from when their owners noticed signs of colic to the horses’ hospital admission). Horses that were eventually euthanized showed clinical signs for an average of 22.2 hours before hospital admission.* residents Drs. Ryan Shoemaker and James Carmalt — reviewed the medical records of more than 700 horses that presented with signs of colic at the WCVM Veterinary Teaching Hospital between 1992 and 2002. Altogether, 604 horses were diagnosed as “true colics” or cases of gastrointestinal colic that were confirmed by standard diagnostic methods. A Western College of Veterinary Medicine study shows that some of our experiences with colic are distinctive from those experienced in other parts of the world. Nearly 55 per cent were medical cases while the rest were classified as surgical colic cases. Besides diagnosis and case origin, the research team collected data regarding age, breed, sex, duration of clinical signs, type of pain control used, heart rate, presence of nasogastric reflux, transrectal examination findings, hospitalization days and case outcome. 3 most common causes among surgical cases: large colon displacement, large colon torsion and strangulating lipoma.* Because this study focused on colic cases in a veterinary teaching hospital population, it’s not an absolute reflection of the types and distribution of various colics that are presented in private and ambulatory clinics across the West. However, a distinct characteristic of the WCVM study was that 36 per cent of the Cause 3 Cause 4 Cause 5 colic cases hadn’t been Large colon Enteritis (9%) Meconium examined by other displacement (10.4%) impaction (7.5%) veterinarians (or “first opinion” cases), while Spasmodic Large colon Other small nearly 64 percent of the colic (12.6%) volvulus (4.2%) intestinal strangulation cases were referred to and peritonitis (3.1% each) the hospital by private Large colon Spasmodic Lipoma (6.4%) practitioners or by volvulus (14.1%) colic (11.5%) WCVM’s Field Service. “Having that Large colon Other small Large colon high a number of displacement (10.8%) intestinal volvulus (7.1%) ‘first opinion’ cases strangulation (8.3%) isn’t common in other TOP 5 CAU S E S O F C O L I C , B A S E D O N AG E 8 Age (years) <1 Cause 1 Large colon impaction (17.9%) Cause 2 Spasmodic colic (16.4%) > 1 to 7 Large colon impaction (27.7%) Large colon displacement (20.5%) > 7 to 15 Large colon displacement (17.9%) Large colon impaction (16%) > 15 Lipoma (28.6%) Large colon impaction (14.3%) Horse Health Lines • Fall 2005 U LT R A S O U N D E x p o s é 93.6 % of the horses that were diagnosed and treated as medical cases survived to discharge. In comparison, 59.6 per cent of the cases that were diagnosed and treated as surgical cases survived to discharge.* veterinary institutions, and that’s a major plus for WCVM students who get to see a full range of cases,” points out Abutarbush. The wide range of cases also added to the quality of the information gleaned from this study. Now, western Canadian veterinarians can use this region-specific data as a diagnostic guide for future colic cases. With that goal in mind, the scientists devised a useful table that highlights the five most common causes of colic in relation to horses’ ages. “As soon as a practitioner receives a colic case, he can look up the five most common causes of colic for that horse’s age category and narrow down the potential causes,” explains Abutarbush. But besides being a diagnostic aid, the study’s results will serve another purpose: “The more veterinarians know about the causes of colic, the more information they can pass on to their clients,” points out Abutarbush. The end result is that western Canadian horse owners will be more aware of the consequences of colic and how detrimental it can be to ignore the disease’s early warning signs. H *Statistics based on results reported in “Causes of gastrointestinal colic in horse in Western Canada: 604 cases (1992-2002).” DIAGNOSTIC TABLE (left): The five most common causes of colic presented to WCVM from 1992 to 2002, sorted by age. Table courtesy of the Canadian Veterinary Journal, 2005; 46(9): 800805. Lucky for horse owners, Dr. Sameeh Abutarbush doesn’t stop thinking about his cases when he goes home. Because it was in the wee hours of one night several years ago — long after the veterinary resident had finished his shift at WCVM’s Veterinary Teaching Hospital — that he thought of a new way to diagnose a deadly type of colic. Large colon volvulus is one of the most devastating causes of colic in horses. Because advanced cases need immediate surgery, early diagnosis of the condition is crucial to survival: “It only takes three to four hours before irreversible damage happens to the colon, so time is critical,” explains Abutarbush, now a board-certified specialist in veterinary internal medicine. An elevated heart rate and an abnormal rectal examination are clinical signs of a horse suffering from large colon volvulus. Early in the course of the disease, veterinarians may encounter an atypical presentation — a horse with a low heart rate, a normal rectal examination and normal results from an abdominocentesis (belly tap). “No one knows why these cases are different: it may be that there hasn’t been enough time for trapped gas to accumulate or the damage to the colon tissues hasn’t happened yet,” says Abutarbush. “The problem is that most veterinarians wouldn’t be keen on recommending surgery for a ‘non-colicky’ horse. But any delay in surgery could mean the difference between saving and losing the patient.” The solution, says Abutarbush, is to use ultrasonography to detect a key difference in the ventral (bottom or lower) and dorsal (top or upper) sides of the large colon. In healthy horses, ultrasound images show the sacculated (lined with sacs) ventral large colon. But in cases where the large colon has twisted, the images will show the non-sacculated dorsal large colon. By taking ultrasound images at two “land mark” points, Abutarbush says practitioners can identify the origin of rotation and estimate how much of the large colon is involved. The diagnostic method has a limitation: if the large colon rotates to exactly 360 or to 720 degrees, the sacculated ventral side of the organ will appear to be in the right place. “But when the large colon twists, chances are it will revolve to points of rotation between those particular degrees,” points out Abutarbush, adding that the ability to identify the point of rotation is extremely valuable. “If you know the length of the colon that’s affected, you can give your patient a much more accurate prognosis.” Abutarbush has successfully used ultrasonography to diagnose large colon volvulus in four colic cases, and those findings are described in an article that’s due to be published in an upcoming issue of the Journal of the American Veterinary Medical Association (JAVMA). While ultrasonography is becoming a common diagnostic tool for colic cases, Abutarbush believes this is the first time anyone has described using this technology to diagnose large colon volvulus based on the organ’s anatomy. Scientists need to do further testing to verify the method’s accuracy, but Abutarbush believes that the new technique will be widely used: “It’s noninvasive, it’s easy to do in the field, it gives veterinarians a better chance of diagnosing large colon volvulus in its early stages and it increases the chances of giving a client a more optimistic prognosis.” Western College of Ve t e r i n a r y M e d i c i n e 9 Front Line SUPPORT T GI Tr a c t Attraction When Dr. Sameeh Abutarbush began a retrospective study of colic cases at WCVM in 2002, the study covered one of the resident’s favourite subjects. Gastrointestinal system problems — especially equine colic — have always held a special attraction for Abutarbush: “I like to think about all of the changes that can happen, how lesions are created and cause such a variety of problems. Sometimes I just have to sit back and think about why did this situation happen? How did it happen?” Fortunately, WCVM provided him with the ideal environment to explore his pet topic. The veterinary teaching hospital’s full range of referred and “first opinion” cases, ready access to an ultrasound machine — plus the support of faculty and fellow graduate students — allowed Abutarbush to initiate several colic studies during his residency. Three years later, Abutarbush has published four colicrelated research articles*, but these recent projects are just a few items on Abutarbush’s to-do list: in the past six months, the veterinarian completed his three-year residency and his Master of Veterinary Science degree at WCVM. Abutarbush also became a diplomate of the American College of Veterinary Internal Medicine this spring. He’s now an assistant professor in the Atlantic Veterinary College’s Department of Health Management at the University of Prince Edward Island. But Abutarbush’s interest in WCVM’s colic cases isn’t over: he plans on searching the study’s enormous database to generate even more details about Western Canada’s experiences with colic. “There are so many factors that I want to try and find an association with — I just find the possibilities fascinating.” *A listing of colic-related articles produced by Abutarbush and his co-authors: • “Using ultrasonography to diagnose large colon volvulus in horses,” by Dr. Sameeh Abutarbush. Journal of the American Veterinary Medical Association (in press). • “Comparison of surgical versus medical treatment of nephrosplenic entrapment of the large colon in horses: 19 cases (1992-2002),” by Drs. Sameeh Abutarbush and Jonathan Naylor. Journal of the American Veterinary Medical Association [2005; 227(4): 603-605]. • “Causes of gastrointestinal colic in horses in Western Canada: 604 cases (1992-2002),” by Drs. Sameeh Abutarbush, James Carmalt and Ryan Shoemaker. Canadian Veterinary Journal [2005; 46(9): 800-805]. • “Strangulation of small intestines by a mesodiverticular band in three adult horses,” by Drs. Sameeh Abutarbush, Ryan Shoemaker and Jeremy Bailey. Canadian Veterinary Journal [2003; 44(12): 1005-1006]. HEY CAME IN THE FALL when the Clement family’s pasture-bred mares were expected to be a few months along in their pregnancy. Arriving by the vanloads, the fourthyear veterinary students from the Western College of Veterinary Medicine were eager to learn more about “pregnancy checking” and to gain hands-on experience. They had no shortage of learning material at the Bar C Ranch near Rossburn, Man. As the large herds poured into the corrals, the students stood awestruck by the sight of dozens and dozens of healthy mares streaming through the gates. “That was a great teaching time for the students since very few of them had ever seen 100, 200, 300 horses at a time,” recalls equine rancher Fred Clement. “Every time they went through the barns and learned how we worked with those large numbers of horses, they gained a basic understanding of the equine ranching industry and how horses are treated on a commercial scale.” Besides providing an ideal training ground for up-and-coming veterinarians, the Clements also allowed WCVM researchers and their collaborators to include large numbers of their horses in a variety of studies. Many of these investigations focused on natural and assisted reproduction, but others also addressed issues surrounding foal management and equine behaviour. Reflecting on F r e d Norm Luba, executive director of the North American Equine Ranching Information Council (NAERIC), talks about Fred Clement and his family’s impact on the equine ranching industry. Q When did you first meet Fred Clement? I visited the Clements’ Bar C Ranch on my first tour when I began with NAERIC in 1995. Without question, Fred was a tremendous inspiration to me personally and professionally. Fred’s vision for the industry was always our inspiration on the board, in the NAERIC office and among our membership. He’s a leader who has a keen way of looking at all aspects of an issue or idea, and of arriving at a common sense approach to that challenge or opportunity. Q Q. How did Fred help develop future leaders? For many years, Fred served as president of the Manitoba Equine Ranching Association and of NAERIC. His creativity and innovative approaches to equine ranching weren’t lost in the board room. Fred earned the respect of his fellow leaders and was a mentor to younger ranchers. He was honest, sincere and truly cared for each rancher. He was willing to spend as much time as necessary on behalf of the industry, often times at significant personal compromise. That research partnership began in the mid-1970s when Fred’s father, Harold, welcomed WCVM theriogenologist Dr. Frank Bristol to the ranch. “I was in my late 20s when Frank came one summer, and I remember how he spent weeks out in the pasture, watching our horses from 4 a.m. to 10 p.m. at night,” recalls Fred. Bristol’s study on pasture breeding behaviour eventually earned him an invitation to present his findings at an international symposium in Australia. It wasn’t the last time the Clement family’s horses contributed to the world’s understanding of equine health: WCVM researchers have presented and published dozens of papers whose research subjects carried the Bar C Ranch brand. “Having access to such a large group of horses on one farm has been an invaluable resource for our research team. While other scientists focused their research on a handful of horses, we designed studies that included hundreds of subjects which dramatically increased the significance of our findings,” explains WCVM researcher Dr. Hugh Townsend. Q How did the Clements’ relationship with WCVM benefit equine ranchers? Fred’s curiosity led him to his significant interaction with research and veterinary education projects at WCVM. His belief in the importance of cutting edge research, in sharing that research with producers and in producing outstanding equine veterinarians will be a lasting legacy to the horse industry. As a staunch promoter of the research check-off program, Fred promoted participation by our ranchers who contributed many thousands of dollars to WCVM. Their support has fueled equine research and education that benefits our industry as well as the entire horse industry. Q What’s significant about the Clements’ support of veterinary education? Fred’s willingness to host numerous veterinary students at his ranch fostered mutually-beneficial relationships between the veterinary profession and the equine ranching industry. I often comment on the fact that the equine ranching industry is the greatest learning opportunity available to academic institutions. In addition, the industry benefits from the science-based management that results from implementing the knowledge derived from veterinarians who study the horses and the industry’s practices. “Results of these studies have led to a greater knowledge of horse health that benefits the entire horse industry — not only in Western Canada but around the world. That couldn’t have been done without the Clement family’s co-operation: veterinarians and horse owners owe this family a great debt.” Meeting new generations of veterinary students and researchers is a ritual that Fred and his wife Lois will miss on their family’s ranch. Last year, the Clements were one of the equine ranching families who left the industry and received a compensation package after Wyeth Pharmaceuticals reduced the PMU (pregnant mares’ urine) producer network in Western Canada and North Dakota. Besides leaving his roles at the Manitoba Equine Ranching Association and the North American Equine Ranching Information Council, Fred resigned from his longtime post as the industry’s representative on the WCVM Equine Health Research Fund’s advisory board (a role his father also filled for 15 years). During his time with EHRF, Fred was a key supporter of a check-off program where equine ranchers contributed a percentage of their income to EHRF. Since that program’s creation, the industry has contributed many thousands of dollars to WCVM’s equine health research and training programs. “What I really enjoyed most about being part of these boards was encouraging people around me to come up with good ideas — to bring out the best in people – so we could deal with some of the challenges that arose,” says Fred, who is proud of his family’s contributions to equine health and to the veterinary profession. “What was always foremost in my mind was the fact that we needed to support the College because it was producing the equine practitioners we really needed in Western Canada,” says Fred. “That was always the strength behind our family’s support for WCVM.” H ABOVE: Fred Clement (centre) visiting friends at the NAERIC display during the EHRF’s 25th anniversary celebrations at Spruce Meadows in 2003. Q Your industry has undergone many changes, but what remains the same? The foundation of what we do relies on optimal management of the horses entrusted to us. Our livelihoods depend on the health and well-being of the horse. We’ll always have a need for equine research and educating equine veterinarians, and our industry will continue to support WCVM’s programs in every way possible. Q What did the Clements contribute to the industry? The Clements contributed to the vision, rationale and implementation of the modernized equine ranching industry. Their vision became the vision of most ranchers. They led by example, and the industry always sought their wisdom to lead them through changing times. It’s hard to imagine equine ranching without the presence, physically, of the Clements. But in spirit, their presence will be felt for many generations. To a large degree, those ranchers that continue in the business owe a great debt of gratitude to the Clements and to others who shaped this industry. For that, we’ll be forever thankful. WCVM CASE REPORT When Dr. Chris Clark first saw the three-year-old Quarter horse mare in August 2005, she looked healthy except for one noticeable problem. “The horse had a foul-smelling, nasty-looking discharge coming from her nose,” explains Clark, a veterinary clinician at the Western College of Veterinary Medicine’s Veterinary Teaching Hospital. A Common Fungus in an Uncommon Place The horse, which was referred to WCVM by Dr. Sean Archibald (WCVM ’94) of Spruce Grove, Alta., had been producing the nasal discharge since May — shortly before the animal was diagnosed and treated for Streptococcus equi infection (strangles). When the horse came to the Okotoks Animal Clinic in late June, Dr. Emma Read (WCVM ’98) noticed white plaque lining the horse’s left nasal passage during an endoscopic examination. Laboratory results confirmed that it was Aspergillus spp. — a common fungus found in hay. An allergic reaction to the fungus’ spores often causes chronic obstructive pulmonary disease (COPD) or heaves, but it rarely colonizes and causes direct disease. In late July, Read’s colleague — Dr. Erin Fierheller (WCVM ’00) of Okotoks Animal Clinic — reexamined the horse and found that the fungal infection was much more extensive. Fierheller physically removed as much plaque as possible, then flushed the area with enilconazole — an antifungal drug. But it was still unclear how much damage the fungus had caused, plus Fierheller had another concern: after hearing abnormal lung sounds, she feared that the infection had developed into fungal pneumonia — a difficult and expensive condition to treat. After discussing the case with Fierheller, Archibald suggested that the owners bring their horse to WCVM. Clark — along with equine surgical specialist Dr. James Carmalt and veterinary intern Dr. Brandy Burgess — listened to the patient’s lungs and performed a transtracheal wash shortly after the horse arrived in Saskatoon. Based on lab results and their findings, the veterinarians ruled out fungal pneumonia. Next, they performed a second endoscopic examination of the nasal passages and confirmed that the enilconazole treatment was working. But they also witnessed a startling sight: large amounts of the horse’s nasal passageway had completely disappeared. “When you look up a horse’s nasal passages with an endoscope, you normally see a narrow passageway that consists of the ventral, middle and dorsal passages,” explains Clark. Consisting of thin nasal bone covered with 12 Horse Health Lines • Fall 2005 mucosal tissues, these passages are like coiled “scrolls” with numerous twists and turns. “The middle passage is usually quite tight and you can’t see very much. But in this case, when we passed the scope up the middle passage, there was no wall left on the outside — just a big open space.” Since endoscopic and radiographic images couldn’t show the extent of the fungus’ spread, Clark, Carmalt and Burgess elected to use computed tomography (CT) to pinpoint the extent of the damage. After anesthetizing the patient, medical imaging specialist Dr. Kimberly Tryon performed a CT scan on the horse’s nasal passageways. These images clearly showed that the damage extended from about two inches into the horse’s nostril to below the eye. “The right side shows the normal, convoluted structure in the nasal passages while the left side shows open space,” describes Clark. “The CT scans defined the limits of the infection, and it allowed us to be more comfortable about making our next decision.” The clinical team drilled a small hole through the anesthetized horse’s skull into the sinus then pushed a tiny endoscope through the hole. Once inside the sinuses, the team members confirmed there were no further signs of disease — a good sign since it lowered the chances of the fungal infection spreading from the sinus to the horse’s brain. The clinicians decided to continue the enilconazole treatments, “but we wanted to make sure that the owners could treat the animal themselves. Plus we wanted to get it into as many corners of the nasal passages as possible,” explains Clark. As well, using a topical treatment avoids serious systemic side effects and reduces costs. After removing the scope from the drilled hole, the team replaced it with a tube. As soon as the horse recovered from general anesthesia, Clark and Carmalt injected the enilconazole solution through the tube. “The solution flowed out into the sinus, through the nasal passages and bathed all of the infected tissues. Our hope was that after two weeks of this once-daily treatment, the enilconazole would destroy the fungus,” says Clark. The horse went home later that day, sporting the tube that emerged out of its skull between its eyes. “We sutured it to the forehead, then it came between the ears where we braided it into its mane,” describes Clark. “All you had to do was walk up to its neck and hold it while you attached a syringe to the tube. Then you injected the solution and let it slowly drip out.” While antifungal medication eliminated the infection, the damage to the horse’s nasal passageway is permanent. But based on other cases where surgeons have removed nasal tumours, horses can manage with a wide open nasal passageway. “Because the nasal chambers help to filter out dust and warm cold air, this horse may be more prone to coughing in a dusty environment or if it’s being exercised in cold. Other than that, it should be fine,” says Clark, adding that the patient still has a promising career as a cutting horse. Was Aspergillus spp. responsible for such widespread damage? As Clark explains, once the nasal bone and mucosal tissue became so infiltrated with the fungus, the horse’s body eventually turned on itself to destroy the fungal infection — a process that produced the putrid nasal discharge. What remains a mystery is how the fungus managed to thrive in the horse’s nasal passageway — a rare occurrence based on the very few cases that Clark could find in recent veterinary literature. And what about the initial S. equi infection: was it an accessory in the fungus’ development? “I don’t think the fungal infection grew because the horse’s immunity was lowered, but I wonder whether there was sufficient damage caused by the strangles to help the fungus gain a foothold in the same area,” says Clark. Whatever led to the fungus’ development, Clark doubts that it will recur. “Even quality hay has some Aspergillus spp. in it, and it’s not an option to completely remove it from a horse’s environment,” says the clinician. “Really, this case was a bit of a lightning strike, and you know how the saying goes — lightning rarely strikes the same place twice.” H LEFT: A CT scan shows the horse’s normal nasal passageway on the right side (left side of this image). In comparison, most of the nasal bone and mucosal tissue has disappeared on the left side (right side of this image). ABOVE: A tube was inserted into a drilled hole in the horse’s head so the owners could continue administering antifungal medication. CLUB Horse Health With six horses living on her family’s acreage near Asquith, Sask., it doesn’t take long for 15-year-old Elinorah Ripley to rattle off a list of troubles that she’s seen in her herd: “We’ve had hoof abscesses and bad gashes, we’ve had babies and old horses. We’ve taken of horses that are easy keepers, hard keepers . . . .” In Ripley’s world, horse health knowledge is an asset. So when she and other members of the Heartland Pony Club heard about a day-long equine seminar at the Western College of Veterinary Medicine (WCVM) last January, they jumped at the chance to go. They weren’t alone. More than 400 4-H and Pony Club members, club leaders and parents braved the icy trip to Saskatoon to hear veterinary students talk about everything from horses’ skeletons, nutrition and colic to common parasites, diseases and lameness issues. For maximum enjoyment, WCVM students split up the young horse enthusiasts — ages 6 to 19 — into three groups and gave presentations that fit each group’s knowledge level. All of the presenters are interested in pursuing a career involving horse health, and many of the 20-plus volunteers were also former Pony Club or 4-H members — experience that came in handy. “We wanted to give the kids information they could take home. The best way to describe it is that we tried to include all of the things we wish we would have known when we were their age,” explains third-year WCVM student Tee Fox, who is also a leader in the Viscount 4-H Light Horse Club. “Some veterinary students were previous A- or B-level Pony Club members, while others worked their way through 4-H so they know quite a bit about both programs,” adds Carol Weiler, the regional education chair for Pony Clubs in Saskatchewan. Having presenters who can directly relate to an audience of young horse enthusiasts adds extra value to these educational seminars — and it’s one Western College of Ve t e r i n a r y M e d i c i n e 1 3 of the main reasons why Weiler has been working closely with WCVM students to organize these kinds of events in the past three years. The additional horse health information helps prepare Pony Club members for their annual examinations as well as the organization’s national quiz program. Horse health knowledge is also a vital part of the 4-H Club’s annual unit examinations. While previous seminars focused on Pony Club members, local 4-H Clubs were also invited to the 2005 event: “It was a good chance for everybody to see that horse health is pretty much the same whether you ride English or Western,” says Fox. “We tend to become isolated when we only attend 4-H-related events, so this was a great opportunity. It gave our club’s members the chance to see and meet students outside of 4-H who are involved in horses,” adds Patti Turk, a leader in the Handel Multiple 4-H Club. Older members in Turk’s group especially enjoyed learning about lameness issues: “It was a good visual presentation 14 Horse Health Lines • Fall 2005 that used video clips to demonstrate how horses react differently to lameness problems,” explains Turk. “The students described the different muscles, tendons and bones in understandable terms, and we came away with some really useful information.” For a fun finalé, veterinary volunteers hosted a game show with multiple-choice questions that covered the day’s topics. “We added in some other interesting questions like, ‘How many ribs does a horse have?’ or ‘How long is a horse’s small intestine?’ — the kinds of things that fascinate kids,” says fourth-year student Tracy Epp. 4-H and Pony Club members weren’t the only beneficiaries: the chance to interact with horse owners was also very valuable for the veterinary volunteers. “We don’t get many chances to practise client communications in our early years,” explains Epp. “Students brushed up on the topics while they prepared lectures — so it was a good experience for all of us.” Buoyed by positive feedback, WCVM students plan to organize another seminar in 2006. As for Ripley, she hopes future seminars will include information on developing sport-specific conditioning programs and on veterinary medicine as a career. “What I like about these seminars is that you’re listening to people who have learned about the subject and have hands-on experience,” says Ripley. “They actually have answers that you know aren’t pulled out of the air or from another book.” “It is the Horse Industry Association of Alberta’s belief that quality research is important to resolving health issues in horses as well as teaching veterinary fellows to become leaders in equine medicine and health research. The association also acknowledges the continued support of the college to the Alberta Horse Breeders and Owners Conference and the horse industry in Western Canada.” — Les Burwash, secretary-treasurer of the Horse Industry Association of Alberta. An excerpt from a letter accompanying the association’s annual EHRF donation. The following list includes the names of all of the Equine Health Research Fund’s contributors from September 1, 2004, to August 31, 2005. The EHRF contributor list is published annually in the fall issue of Horse Health Lines. $5,000-plus • British Columbia Standardbred Breeders’ Society, SURREY, BC • Dube, David, SASKATOON, SK • Horse Racing Alberta, EDMONTON, AB • North American Equine Ranching Information Council (NAERIC), LOUISVILLE, KY • Saskatchewan Horse Racing Commission, SASKATOON, SK $1,000 to $4,999 • Anonymous • Canadian Thoroughbred Horse Society, WINNIPEG, MB • Caron, Dr. John, LANSING, MI • Chouinard, L.D., DEWINTON, AB • Horse Industry Association of Alberta, AIRDRIE, AB • Manitoba Jockey Club Inc., WINNIPEG, MB • Roper, G.F., CALGARY, AB • Townsend, Dr. Hugh, SASKATOON, SK • Up to $999 • A • Anonymous • B • BMO Fountain of Hope, TORONTO, ON • BMO Nesbitt Burns Inc., TORONTO, ON • Baber, Dorothy, BALCARRES, SK • Bailey, M.E., PRINCE ALBERT, SK • Barber, Dr. Spencer, SASKATOON, SK • Bassett, Georgina, VICTORIA, BC • Bergmann, D., EDMONTON, AB • Big Hill Veterinary Services, COCHRANE, AB • Bird, Donna, CALGARY, AB • Boghean, Ronald, CALGARY, AB • Boucher, B., BRANDON, MB • Boulware, M.C., CALGARY, AB • Burlingame, D.M., SASKATOON, SK • Burns, Bev, EDMONTON, AB • C • Cadman, D.M., AIRDRIE, AB • Campbell, K., CALGARY, AB • Carroll, L.D., RUMSEY, AB • Charlton, K.W., BOWDEN, AB • Chart, Christopher, NELSON, BC • Clark, Dr. Ted, SASKATOON, SK • Clarke, D., BALZAC, AB • Cohen, Cheryl, CALGARY, AB • Colchester & District Agricultural Society, SHERWOOD PARK, AB • Cole, H.W., OLDS, AB • Collins, K.Y., ROSETOWN, SK • Cook, Cliff, BOWSMAN, MB • Corbett, Q.C., B., CALGARY, AB • Coulthard, C., CASTOR, AB • Cribb, Peter, SASKATOON, SK • Croken, G., PONOKA, AB • Crush, Ken, LANGHAM, SK • D • Dawson Creek Vet Clinic Ltd., DAWSON CREEK, BC• Dechant, Sheila, Federation Inc., REGINA, SK • Saskatchewan Regional Pony Club, SASKATOON, SK • Saskatchewan Association of Veterinary Technologists, ABERDEEN, SK • Saskatoon Pony Club, CLAVET, SK • Schneidmiller, H., CALGARY, AB • Silver Spur Riding Club, ERRINGTON, BC • Smith, Eva M.E., REGINA, SK • Smith, Hannelore, SASKATOON, SK • Souris Valley Trekkers, ESTEVAN, SK • Southern, M.E., CALGARY, AB • Stair, E.D., COCHRANE, AB • Steinman, T., BALZAC, AB • Stelmaschuk, M., EDMONTON, AB • T • Theilman, Laura, SASKATOON, SK • Tishauser, D., CALGARY, AB • Townsend, R. D., VICTORIA, BC • U • United Way of Calgary, CALGARY, AB • W • Walker, D.R., OKOTOKS, AB • Wallace, J., DAUPHIN, MB • West Central Arabian Association, SASKATOON, SK • Weston, B., SASKATOON, SK • Wheels and Saddles Driving & Riding Club, WAWOTA, SK • Whitley, Janet and Jaclyn, SASKATOON, SK • Willow Ridge Pony Club, SASKATOON, SK • Wolfe, M.R., CALGARY, AB • Wood, J.R., REGINA, SK • Wood, M., CALGARY, AB • Z • Zeilner, Catherine, CASA RIO, SK • Zurawski, C.D., REGINA, SK. A YEAR IN REVIEW OUR CONTRIBUTORS EDMONTON, AB • Duncan, R.A., ROCKHAVEN, SK • Dykes, Mary, SASKATOON, SK • Dzierzanowski-Cerato, W.M., AIRDRIE, AB • E • Echlin, S.L., SASKATOON, SK • Edworthy, J., CALGARY, AB • Elaschuk, N.A., TURIN, AB • Elder, Brian and Sharon, SASKATOON, SK • Erickson, Gwen, CLAVET, SK • F • Fisher’s Drug Company Inc., NORTH BATTLEFORD, SK • Fitzharris, Fern, SASKATOON, SK • Foxleigh Riding Club, REGINA, SK • Frank’s Saddlery & Supply Ltd., LLOYDMINSTER, SK • Frolic, N.K., COMOX, BC • G • Gaehring, S., CALGARY, AB • Gawley, S.R., SASKATOON, SK • Gladson, D.L., PICKARDVILLE, AB • Gould, Kay, PRINCE ALBERT, SK • Gray, Lorna, WINNIPEG, MB • Gregory, M., LANGLEY, BC • H • Halina, K.J., SASKATOON, SK • Hamilton, Dr. Donald, SASKATOON, SK • I • Isman, V., GLADSTONE, MB • J • Johnson, Sharon, SASKATOON, SK • Johnson, W., PRIDDIS, AB • Jones, G.D., CALGARY, AB • JustAnotherFarm, KATHYRN, AB • K • Kanevsky, J., COURTENAY, BC • Kessler, Marlise, COCHRANE, AB • Killeen, James Randy, SHERWOOD PARK, AB • Klein, I., EATONIA, SK • Knight, D., STRATHMORE, AB • Koosey, P., CALGARY, AB • L • LaPlante, J.L., CALGARY, AB • Langlois, S., EDMONTON, AB • Lee, J.W., PENTICTON, BC • Lenz, S.B., CALGARY, AB •Lepoudre, L., AIRDRIE, AB • Loney, R., CALGARY, AB • Longair, J., DUNCAN , BC • Lynch, Lorne, DAVIDSON, SK • M • Mackie, D., CALGARY, AB • Manitoba Trail Riding Club Inc., ST. ADOLPHE, MB • Matheson, G., LANGLEY, BC • Maxwell, Allison, MAPLE RIDGE, BC • McCargar, Murray, CALGARY, AB • McClellan, A., VICTORIA, BC • McKague, Brenda and Ross, BRANDON, MB • Metzger-Savoie, P., CALGARY, AB • Misra, Vikram, SASKATOON, SK • Moore & Co. Veterinary Services, BALZAC, AB • More, Everett, VIRDEN, MB • N • Nelson & District Riding Club, NELSON, BC • Nordstrom, Glenn, VIKING, AB • O • Okotoks Animal Clinic Ltd., OKOTOKS, AB • P • Palese, K.M., CALGARY, AB • Palouse Holdings Ltd., CALGARY, AB • Paton, Dr. David, ALDERGROVE, BC • Pauw, Kathy and Mendelt, SASKATOON, SK • Perry, C.M., RED DEER, AB • Q • Quesnel & District Riding Club, QUESNEL, BC • Quinn, R., BANFF, AB • R • Racine, R., ABBOTSFORD, BC • Regina District Dressage Assoc., REGINA, SK • Riddell, Betty (In Memory of Murray Riddell), SASKATOON, SK • Robinson, Brian, LLOYDMINSTER, SK • Rossmann, T., OKOTOKS, AB • Runge, Wendy, CALGARY, AB • S • Saskatchewan Horse The Equine Health Research Fund’s statement of revenue, expenditures and fund balances fro the year ended December 31, 2004. EXPENDABLE Revenue Donations Private Horsemen’s Association Racing Commissions NAERIC Miscellaneous Expenditures Fellowship Program Grants Cost recovery from previous grants Summer student Fund raising Horse Health Lines Administration - Advisory Board Equipment Excess (deficiency) of revenue over expenses Transfer from restricted funds Fund balance, beginning of year Fund balance, end of year RESTRICTED 2004 2003 $23,687.35 12,586.00 30,000.00 28,900.00 163.32 $43,278.61 8,500.00 30,250.00 31,300.00 0.00 $95,336.67 $113,328.61 50,605.79 85,586.00 (7,884.01) 7,700.00 12,646.75 25,834.99 2,860.14 0.00 31,791.66 40,013.00 (11,403.99) 0.00 22,078.67 36,510.55 3,433.31 11,716.71 $177,349.66 $134,139.91 (82,012.99) 74,669.69 8,801.31 (20,811.30) 54,456.00 (24,843.39) $1,458.01 $8,801.31 2004 2003 Investment income Transfer to unrestricted fund Fund balance, beginning of year $144,549.14 (74,669.69) 1,646,935.33 $142,442.63 (54,456.00) 1,558,948.70 Fund balance, end of year $1,716,814.78 $1,646,935.33 Western College of Ve t e r i n a r y M e d i c i n e 1 5 HORSE HEALTH ON TRACK: More than 50 horse G A L L O P I N G G A Z E T T E ARTHROSCOPY THERAPY: According to a Western College of Veterinary Medicine clinical case report, veterinarians should consider using standard arthroscopic equipment to evaluate and treat temporomandibular joint (TMJ) sepsis in horses. This joint is part of the horse’s cranium where the mandible (jaw) contacts and articulates with the temporal bone — the area of the skull where the horse’s ear resides. Drs. James Carmalt and David Wilson, two equine surgical specialists at WCVM’s Veterinary Teaching Hospital, examined a 12-year-old Thoroughbred mare affected by TMJ sepsis — a rare condition in horses. The specialists made a stab incision into the dorsocaudal compartment, then used arthroscopy to investigate the dorsal joint pouch of the right TMJ. After initial evaluation, the WCVM team used arthroscopic equipment to debride and thoroughly clean the joint. As well, the specialists administered intra-articular and systemic antibiotics to treat the horse’s infection. The team successfully resolved the horse’s joint sepsis with the combination therapy. Eight months later, the specialists found no clinical evidence of degenerative joint disease or ankylosis of the TMJ. Based on those promising results, Carmalt and Wilson believe that arthroscopic debridement and lavage should be considered for evaluation and initial treatment of TMJ sepsis in horses. RIDE FOR HORSE HEALTH: As Saskatchewan marked its 100th anniversary this summer, a provincial group created its own centennial celebration along the banks of the South Saskatchewan River. On September 3, 37 horse and rider teams from across Western Canada took part in the Saskatchewan Long Riders’ annual ride in the Nisbet Provincial Forest, about 80 kilometres north of Saskatoon, Sask. Riders competed in a 25-mile limited distance ride, or in one of two endurance races: a 50mile or a 100-mile circuit. Myna Cryderman and Night Skye of Manitoba earned first place as well as the ride’s best condition with a time of nine hours and 45 minutes. Fellow Manitoban Dawn Crowley and her horse Blade finished first and earned best condition in the 50-mile race, while Alice Gaucher and her horse Zip won first place and best condition in the limited distance ride. As in the past, the Saskatchewan Long Riders are generously donating all proceeds of the ride to the Equine Health Research Fund. To add to the contribution, WCVM’s Drs. Don Hamilton, James Carmalt and Jenny Kelly waived their fees for conducting the ride’s mandatory veterinary checks. Third-year veterinary students Jen Fowlie, Jane Harms, Barb Wesselowski and Julie Bulman-Fleming also assisted the ride’s veterinary team. *Arthroscopic treatment of temporomandibular joint sepsis in a horse, published in Veterinary Surgery, 34 (1): 55-58, 2005. Authors: Drs. James Carmalt and David Wilson. V i s i t H o r s e H e alth Lines on line at www.ehrf.usask.ca PUBLICATIONS MAIL AGREEMENT NO. 40112792 RETURN UNDELIVERABLE CANADIAN ADDRESSES TO: Research Office, WCVM University of Saskatchewan 52 Campus Drive Saskatoon, SK S7N 5B4 wcvm.research@usask.ca owners, breeders, trainers, veterinarians, farriers and racing fans attended an afternoon of horse health seminars at Winnipeg’s Assiniboia Downs on August 13. Organized by the Equine Health Research Fund, the Manitoba Jockey Club and the Manitoba branch of the Canadian Thoroughbred Horse Society (CTHS), the event gave horse enthusiasts and WCVM scientists a chance to discuss some common horse health issues in Western Canada’s racing industry. During the two-hour seminar, equine surgical specialist Dr. Spencer Barber talked about some of the risk factors associated with injuries on the racetrack. Besides his clinical, teaching and research work at WCVM, Barber is authority veterinarian at Marquis Downs in Saskatoon, Sask. The afternoon’s second speaker was Dr. Dave Wilson, an equine surgical specialist and department head of WCVM’s Large Animal Clinical Sciences. Wilson’s topic was the treatment of angular deformities in foals — a key research area for Wilson and his graduate students. Infectious disease specialist Dr. Hugh Townsend and veterinary pathologist Dr. Andy Allen were also on hand to answer questions about the College’s equine research program. Based on their backgrounds and their expertise, the event’s two speakers were ideal resources for the racing audience. “Drs. Barber and Wilson both grew up in southern Saskatchewan, they’ve been around horses all of their lives, they both trained to become surgical specialists and they’re now part of WCVM’s faculty,” says EHRF chair Dr. Andy Allen. “I think they’re great examples of how the Equine Health Research Fund has created ‘a critical mass’ of knowledgeable people at the College who expose undergraduate and graduate students to a wide range of horse health issues, research and training every day.” EHRF PURSE: WCVM’s Equine Health Research Fund was honoured in Assiniboia Downs’ sixth race on Saturday, August 13. Toubeeb and jockey Larren Delorme ran the six-furlong race in 1:51:3 — earning the lion’s share of the $8,400 purse. The $12,000 claiming race was for three-yearolds and older. WCVM’s Drs. Spencer Barber and Andy Allen joined trainer Tom Gardipy Jr. and Toubeeb’s owners in the winner’s circle after the race. Photo: Assiniboia Downs