Fluorochrome chart
FL
450/50
530/30
585/40
630/30
670/40
720/40
780/60
RB
R-PE-Cy7
Cy7
APC-Cy7
AF700
APC-Cy5.5
PerCP-Cy5.5
DRAQ5
PerCP
AF647
R-PE-Cy5.5
R-PE-Cy5
Legend
Cy5
Fluorescent Channel (FL)
■ Blue Channel
■ Green Channel
■ Yellow Channel
■ Orange Channel
■ Red Channel
■ Far Red Channel
APC
7-AAD
TR
Relative Brightness (RB)
High
AF594
Medium
R-PE-TR
Low
PI
Spectra
AF568
Excitation
Emission
R-PE
Lasers
u Violet laser (405 nm)
u Blue laser (488 nm)
u Yellow laser (561 nm)
u Red laser (640 nm)
u Far Red laser (750 nm)
AF555
Cy3
FITC
Abbreviaions
AF488
AF: Alexa Fluor®
BV: Brilliant Blue™
Cy: Cyanine
PB: Pacific Blue™
FITC: Fluorescein isothiocyanate
R-PE: R-Phycoerythrin
PI: Propidium Iodide
TR: Texas Red®
7-AAD: 7 AminoacLnomycinD
APC: Allophycocianin
PerCP: Peridinin Chlorophyll
GFP
PB
BV421
AF405
DAPI
350
400
450
500
How to use this chart
Examples
1 Check your instrument:
Type, number of lasers, filters and detectors dictate the fluorochromes that can be used.
Try to choose a fluorochrome for each laser excitation range
Fluorochrome
2 Select bright dyes:
It is possible to rank available dyes according to their brightness on a particular instrument.
Brightest fluorochromes for dim antibodies and vice versa
3 Minimize spillover:
The amount of spectral overlap will determine whether compensation is required.
Sacrifice brightness to avoid spillover
Avoid spillover from bright cell populations into detectors requiring high sensitivity for those populations
Discover more at abcam.com/fluorochrome-chart
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
550
Target Expression
600
Lasers
650
Channels
700
750
Brightness
Compensation
800
850
Combination
FITC
APC
High
Low
Blue
Red
Green
Red
Medium
High
Mild
FITC
PE
High
Low
Blue
Yellow
Green
Yellow
Medium
High
Moderate
PerCP
7-AAD
High
Blue
Red
Low
Poor
Severe
Low BlueRed Medium
(not recommended)
Good
Medium
E-583
Wavelength (nm): 300