'HOBBLE CREEK' MOUNTAIN BIG SAGEBRUSH SEED PRODUCTION Bruce L. Welch Fred J. Wagstaff Gary L. Jorgensen ABSTRACT 'HOBBLE CREEK' BIG SAGEBRUSH This study measured the seed production of 40 randomly selected 'Hobble Creek' mountain big sagebrush (Artemisia tridentata ssp. vaseyana) plants growing in a seed increase garden. Results indicate that 2- and 3-yearold 'Hobble Creek' plants planted on a 7- by 7-ft grid produce about 72 to 67 pounds of certified pure live seed per acre. There was significant variation among plants within a year and significant variation between years for a given plant. At $20 to $50 per pound of pure live seed, the gross income per acre would be between $1,340 and $3,600. 'Hobble Creek' mountain big sagebrush (Artemisia tridentata ssp. vaseyana) is a superior selection of big sagebrush (Welch and others 1986). It is the only big sagebrush, to date, to be named and released as an improved shrub for use on mule deer and domestic sheep winter ranges (Welch and others 1987). Agencies involved in the naming and releasing of 'Hobble Creek' were: Utah Agricultural Experiment Station, Utah State University; U.S. Department of Agriculture, Forest Service, Intermountain Research Station; Utah State Division of Wildlife Resources; Upper Colorado Environmental Plant Center; Colorado Agricultural Experiment Station, Colorado State University; Idaho Agricultural Experiment Station, University of Idaho; and U.S. Department of Agriculture, Soil Conservation Service. The naming and release of 'Hobble Creek' mountain big sagebrush for commercial production and marketing of seed and plants represent a significant step forward in making big sagebrush available as a forage plant for wildlife and certain domestic livestock. Of 186 big sagebrush selections tested, 'Hobble Creek' was the most preferred by wintering mule deer and was ranked in the most preferred group by wintering domestic sheep (Welch and others 1986). This high degree of preference is the main justification for claiming it as a superior selection of big sagebrush. 'Hobble Creek' ranked in the top third of all selections tested for productivity Oength ofleader growth) and was third out of 20 selections in vegetative production expressed as gram per centimeter of stem (Davis and Stevens 1986; Welch and others 1986). Winter nutritive content of vegetative tissue is: (1) in vitro digestibility52.6 percent of dry matter, (2) crude protein-11.0 percent of dry matter, (3) phosphorus-0.21 percent of dry matter, and (4) above the maintenance requirement for carotene (Welch and others 1986). Nutritionally, 'Hobble Creek' mountain big sagebrush ranks very high among winter forages. Only other selections of big sagebrush rank higher in nutritive value, but they are not as preferred by the consuming animals (Welch 1983; Welch and others 1986). 'Hobble Creek' does not contain substances that lower grass cell wall digestion in ruminant animals (Hobbs and others 1986). 'Hobble Creek' can be established and maintained on sites that have deep, well-drained soils with an annual precipitation of at least 14 inches and preferably more. Soil textures should not be any finer than a clay loam (40 percent clay or less). Soil pH may vary from 6.6 to 8.6 (Welch and others 1986). INTRODUCTION On mule deer (Odocoileus hemionus hemionus) and domestic sheep (Ovis aries) winter ranges, most forages are deficient in four nutrients required by the animals: energy-producing compounds, protein, phosphorus, and carotene (Dietz 1972; Welch 1983; Welch and others 1986). One species of range plant that can supply the four nutrients at or above the maintenance requirements of wintering mule deer and domestic sheep is big sagebrush (Welch 1983). The evergreen nature of big sagebrush gives it a winter nutritional advantage over deciduous shrubs such as antelope bitterbrush (Purshia tridentata), true mountain-mahogany (Cercocarpus montanus), and most herbaceous species (Welch 1989). Big sagebrush can not only increase the nutrient level of winter diets, but is a more dependable forage source during periods of drought than are other shrubs, forbs, or grasses (McArthur and Welch 1982; Medin and Anderson 1979). Big sagebrush also usually remains available for use above the snow while other forage classes become covered and unavailable for wintering animals (Gade and Provenza 1986). However, not all big sagebrush selections are equal in terms of animal preference, growth rate, nutritive valqe, or chemical composition (Welch and others 1986). Paper presented at the Symposium on Cheatgrass Invasion, Shrub DieOff, and Other Aspects of Shrub Biology and Management, Las Vegas, NV, April 5-7, 1989. Bruce L. Welch is a Principal Research Plant Physiologist, and Fred J. Wagstaff is a Range Economist at the Intermountain Research Station, Forest Service, U.S. Department of Agriculture, Provo, UT 84606; Gary L. Jorgensen is a Range Technician at the Intermountain Research Station, Ephraim, UT 84627. 167 This file was created by scanning the printed publication. Errors identified by the software have been corrected; however, some errors may remain. 'Hobble Creek' can be established by direct seeding, by transplanting bareroot or containerized stock, and by a technique we term "mother plant." It is the production of seed needed for the establishment by the direct seeding technique that is the subject of this study. What follows is a description of establishing a seed increase garden of 'Hobble Creek' and results of the first 3 years of production. Next, the medium was cooled, moved into a clean potting room and placed in Tinus root-trainer books (21.5 in3 ; 1.5 by 2 by 7.25 inch). Tinus and other brands of roottrainer books having longitudinal grooves prevent spiraling and reduce balling of the roots. Also, the root tips are air pruned, which aids in reducing root balling. These root trainers were placed in sets of eight into wooden crates and filled firmly to 1 inch from the top. This space provided a water basin. We sowed about five to eight 'Hobble Creek' big sagebrush seeds on the surface of the plant-growing medium and covered the seed with number 3 sandblasting grit 1/s-inch deep (Jacobson and Welch 1987). A fogger was used in the mornings and evenings to keep the seed moist until 2 weeks after germination. Six weeks after germination, seedlings were thinned to one per container. We recommend starting the containerized stock in May in a greenhouse, placing the stock outside during the last part of August, then planting on the site just as soon as the soil is frost free. This takes advantage of early spring (April) moisture. However, for this study containerized stock was started in January and transplanted to the garden in May. We did this to speed up garden establishment. Planting containerized stock in May meant that the stock needed watering during the summer months of June, July, and August. By following the stated recommendations, summer watering can be avoided. Containerized stock of 'Hobble Creek' was transplanted to the garden in May 1986. Planting was done on a 7- by 7-ft grid. This equates to about 900 plants per acre. The 7-foot grid allowed a garden tractor to be used for weed control. We planted about 1,800 plants. From these plants, 40 were chosen at random to study seed production. Each plant was labeled with a metal tag attached to a wooden stake. When the seeds were ripe (mid-November), each plant was harvested with hand clippers. Seed stalks from individual plants were placed in separate plastic bags. The bags were opened and the SITE The garden site is located 4 miles south of Nephi, UT, at the Utah State University, Nephi Agriculture Experimental Station. Soils of this site are of the Nephi series (Nephi silt loam). This soil is a deep, well-drained alluvium derivative of sandstone, limestone, and shale. Soil permeability is slow with an effective rooting depth of 5 ft or more. The pH range is from 8.2 to 8.6. Average annual precipitation ranges from 14 to 16 inches. Frost-free periods are 100 to 140 days. Clay content varies from 15 to 35 percent (Trickier and Hall 1984). The garden site was first used to raise small grains. An orchard offour-wing saltbush (Atriplex canescens) was established on the site, but it was winter-killed about 3 years later. The site was then planted with crested wheatgrass (Agropyron desertorum) for about 6 years before the present seed garden was established. Garden preparation was accomplished by killing the grass with chemicals and tilling with a springtooth tiller. Containerized stock was used for establishing the seed increase garden. Seed was obtained from the 'Hobble Creek' breeder block located in the mouth of Hobble Creek Canyon. MATERIALS AND METHODS In producing containerized 'Hobble Creek' stock, we used a plant-growing medium composed of sphagnum peatmoss, horticultural vermiculite, sandy loam soil, and number 3 sandblasting grit. We screened the peatmoss and soil through 0.5-inch hardware cloth prior to mixing. The plant-growing medium formulation was four parts screened peatmoss, three parts vermiculite, three parts screened soil, and three parts of number 3 sandblasting grit. An alternate formulation is four parts peatmoss, four parts vermiculite, two parts "Turface," and three parts of number 3 sandblasting grit. Regardless of the formulation used, a package of fertilizer was added during mixing of the medium. Table 1lists the fertilizers and the quantities used per cubic foot of plant growing medium. Plant-growing medium and fertilizer were thoroughly mixed while dry in a concrete mixer. Next, water was added until the mixture maintained a ball shape after being hand squeezed. After mixing, the medium was transferred to a soil cart where it was treated with aerated steam. This treatment heated the medium to 175 °F for 30 min. Aerated steam treatment controls many soil-borne plant pathogens. We used the methods described by Nelson (1984) to produce disease-free containerized stock. Table 1-Fertilizer mixture used for the production of containerized 'Hobble Creek' big sagebrush stock. The amount of fertilizer indicated is for each cubic foot of plant growing medium Fertilizer Amount Grams Dolomite Ground limestone Horticultural gypsum Calcium nitrate Osmocote (slow release fertilizer N,P,K) Super phosphate Trace elements Chelate iron-138 168 176 176 100 32 32 26 5 2 seed stalks air dried in a heated greenhouse for about 2 weeks. To prevent the formation of mold, seed stalks were stirred daily within the bags. After drying, the seed and chaff were separated from the stalks by hand. Next, the seed and chaff were screened to remove the larger particles of chaff and further separated by use of an air seed cleaner. Seed purity and percent oflive seed (tetrazolium test) were determined for all 40 seed lots. Production is expressed as grams of pure live seed per plant. Table 2--Seed production for forty 2- and 3-year-old 'Hobble Creek' mountain big sagebrush plants. All data expressed as grams of pure live seed per plant Plant number 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 RESULTS The data from this study are given in table 2. Pure live seed per plant for 2-year-old plants varied from 0 to 136.6 grams. The mean was 36.3 grams of pure live seed, with a standard deviation of 36.6 grams. This would equate to a yield of about 72.0 pounds of pure live seed per acre (900 plants per acre). 'Hobble Creek' seed has sold for $20 per pound of pure live seed. Gross income per acre per year would be about $1,440. (First harvest is about 18 months from transplanting.) Pure live seed per plant for 3-year-old plants varied from 0 to 112.8 grams, with a mean of 33.9 grams and a standard deviation of29.9 grams. This would equate to a yield of about 67 pounds of pure live seed per acre (900 plants per acre). Gross income per acre per year for 3-year-old plants would be $1,340. Raising 'Hobble Creek' seed is a potentially profitable venture. Two major benefits are: First, 'Hobble Creek' seed could be produced on sites that may not be tillable, such as rocky soils or steep slopes (so-called waste land), and second, because of the late date of seed maturity (mid-November) harvesting of seed occurs at a time when for most farming operations labor is at a surplus. Harvesting seed is a labor-intensive operation. We recommend the following for establishing a 'Hobble Creek' seed garden: 1. Plant containerized stock as soon as the frost has left the soil. 2. Establish plant spacing at either 7 or 3.5 feet. 3. For areas with wintering mule deer, build deer-proof fence. Deer browsing can reduce seed stalk production (Rodriguez and Welch 1989; Wagstaff and Welch in press). 169 Seed ~roduction 2-year-old b~ age class 3-year-old - - - - - - - Grams - - - - - - 68.1 14.8 10.0 37.4 0.6 41.8 78.5 1.3 23.1 56.1 3.1 29.8 39.5 45.3 35.4 19.0 57.2 3.4 108.0 10.9 3.2 52.6 126.2 dead 11.0 0.2 62.8 44.3 79.0 11.1 0.5 1.6 2.4 34.2 0.2 77.4 47.4 41.0 33.7 0.6 23.3 4.3 42.3 61.9 40.5 40.6 37.4 77.7 18.9 14.8 0.0 112.8 70.5 97.3 75.4 42.8 92.8 6.5 136.6 11.7 4.3 29.3 17.7 56.7 4.0 3.2 8.9 48.1 20.2 55.0 100.7 17.6 51.9 44.3 0.0 16.0 0.5 8.3 0.0 0.2 REFERENCES area: Proceedings: Intermountain Nurseryman's Association 1983 conference; 1983 August 8-11; Las Vegas, NV. Gen. Tech. Rep. INT-168. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Forest and Range Experiment Station: 32-38. Rodriguez, R. L.; Welch, B. L. 1989. Effects of heavy grazing by mule deer on 'Hobble Creek' mountain big sagebrush seed stalk production. In: Wallace, A., McArthur, E. D.; Haferkamp, M. R., compilers. Proceedings-symposium on shrub ecophysiology and biotechnology; 1984 June 30-July 2; Logan, UT. Gen. Tech. Rep. INT-256. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Research Station: 141-143. Trickier, D. L.; Hall, D. T. 1984. Soil survey of FairfieldNephi Area, Utah-Parts of Juab, Sanpete, and Utah Counties. Provo, UT: U.S. Department of Agriculture, Soil Conservation Service. 361 p. plus 130 maps. Wagstaff, F. J.; Welch, B. L. [In press.] Seedstalk production of mountain big sagebrush (Artemisia tridentata ssp. vaseyana) enhanced through short-term protection from heavy browsing. Journal of Range Management. (Submitted). Welch, B. L. 1983. Big sagebrush: nutrition, selection, and controversy. In: Johnson, K. L., ed. Proceedings, first Utah shrub ecology workshop; 1981 September 9-10; Ephraim, UT. Logan, UT: Utah State University, College of Natural Resources. 50 p. Welch, B. L. 1989. Nutritive value of shrubs. In: McKell, Cyrus M., ed. The biology and utilization of shrubs. San Diego: Academic Press: 405-424. Welch, B. L.; McArthur, E. D.; Nelson, D. L.; Pederson, J. C.; Davis, J. N. 1986. 'Hobble Creek'-A superior selection oflow-elevation mountain big sagebrush. Res. Pap. INT-370. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Research Station. 10 p. Welch, B. L.; McArthur, E. D.; Young, S. 1987. Notice of the naming and release of 'Hobble Creek' mountain big sagebrush. Provo, UT: U.S. Department of Agriculture, Forest Service, Intermountain Research Station. 6 p. Davis, J. N.; Stevens, R. 1986. Comparison of production in 27 accessions of sagebrush taxa. In: McArthur, E. D., Welch, B. L., compilers. Proceedings-symposium on the biology of Artemisia and Chrysothamnus; 1984 July 9-13; Provo, UT. Gen. Tech. Rep. INT-200. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Research Station: 336-431. Dietz, D. R. 1972. Nutritive value of shrubs. In: McKell, C. M.; Blaisdell, J.P.; Goodin, J. R., eds. Proceedings, Wildland shrubs-their biology and utilization; 1971 July; Logan, UT. Gen. Tech. Rep. INT-1. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Forest and Range Experiment Station: 289-302. Gade, A. E.; Provenza, F. D. 1986. Nutrition of sheep grazing crested wheatgrass versus crested wheatgrassshrub pastures during winter. Journal of Range Management. 39: 527-530. Hobbs, N. T.; Welch, B. L.; Remington, T. E. 1986. Effect of big sagebrush on in vitro digestion of grass cell wall. In: McArthur, E. D.; Welch, B. L., compilers. Proceedings-symposium on the biology of Artemisia and Chrysothamnus; 1984 July 9-13; Provo, UT. Gen. Tech. Rep. INT-200. Ogden, UT: U.S. Department of Agriculture, Forest Service, Intermountain Research Station: 186-189. Jacobson, T. L. C.; Welch, B. L. 1987. Planting depth of 'Hobble Creek' mountain big sagebrush seed. Great Basin Naturalist. 47: 497-499. McArthur, E. D.; Welch, B. L. 1982. Growth rate differences among big sagebrush (Artemisia tridentata) accessions and subspecies. Journal of Range Management. 35: 396-401. Medin, D. E.; Anderson, A. E. 1979. Modeling the dynamics of a Colorado mule deer population. Wildlife Monographs. 68. 77 p. Nelson, D. L. 1984. Toward producing disease-free container-grown native wildland plants. In: The challenge of producing native plants for the Intermountain 170