Biochemistry 462 Spring 2000 Hour exam I Name: ______________ 1. ( 25 points) Describe, in as much detail as possible the electron transport system of eukaryotic mitochondria. Include such details as names of the various transport complexes, how the complexes interact with each other, chemical reactions, stoichiometry of reactions, electron carriers, coenzymes, and proton transport events. A diagram would be extremely helpful 2 2. (12 points) Lipids (A) Make a diagram of a simple triacyl glycerol (B) Make a diagram of a simple phospholipid containing serine ( C) What is an eicosanoid and why are eicosanoids important? (D) What is a steriod and why are steroids important? 3. (13 points) What are the structural differences you would expect to see between a ‘normal’ water soluble protein and a membrane bound protein? List as many differences as possible, focusing on structure at 1o,2o, and 3o levels. What forces are responsible for these different structures? 3 4. (25 points) I have a transport enzyme that allows a cell to actively transport maltose into the cell. The enzyme seems to work wether the energy charge of the cell is high or low, as long at the pH of the medium is lower than the pH of the cell. Propose a simple mechanism for this enzyme. Use a step-bystep diagram to show how your mechanisms works. Include in your mechanism a such details as binding events, and conformational changes. 4 5. (15 points) Draw the guanine and cytosine showing the normal Watson-Crick hydrogen bonds that form between these bases in the DNA double helix. 6. (10 points ) Diagram and discuss the physical structure of the E. coli chromosome.