DomainsoftheNucleus(Q)
100:Locationofsplicingfactors.
200:Theresearcherthatproposedthateach
chromosomeoccupiesadistinctterritory.
300:Theprocessofimmobilizing
chromosomesonaslide,denaturingthem,
andthenaddingasingle-stranded
fluorescently-labeledDNAprobethatis
complementarytooneormore
chromosomes.
400:TheformofDNAthathasalowergene
densityandisreplicatedlaterinSphase.
500:Identifythewhite,black,andgolddots
inthefigureshown.(Figureonlastpageof
document)
600:ThemostabundantformofRNA.
Thenucleolus(Q)
100:Thenucleolusformsaroundthearrays
of5.8S,18Sand28SrRNAgenes,whichare
calledwhat.
200:The5SrRNAgenesarelocatedhere,
andtranscribedbythisRNAPolymerase.
300:Thesplicingofthe45Spre-rRNA
transcriptisperformedbythis.
400:Thethreedistinctregionsofthe
nucleolus.
500:Functionsofthenucleolus.
600:Thisfigureisshowingwhatprocess.
Whatarethebranchesandwhatarethe
ornamentsusingtheChristmastreeanalogy.
(Figureonlastpageofdocument)
DomainsoftheNucleus(A)
100:Whatareinterchromosomaldomains?
200:WhoisCarlRabl?
300:WhatisFISH(Fluorescenceinsitu
hybridization)?
400:Whatisheterochromatin?
500:Whataretranscriptionallyactivegenes
(white),transcriptionallysilentgenes(black),
andspicingmachineryorspeckles(gold)?
600:WhatisrRNA?
Thenucleolus(A)
100:WhatareNORs(NucleolarOrganizing
Regions)?
200:WhatisChromosome1andRNA
PolymeraseIII?
300:TrickQuestion!45SpreRNAtranscripts
areprocessed,notspliced(butwhatdoesthe
processing)?
400:Whatarethefibrillarcenter,dense
fibrillarregionandgranularzone?
500:WhatarerRNAtranscription,rRNA
processing,andribosomalsubunitassembly?
600:Figureshows45SrRNAtandemarrays,
ThebranchesarerRNAtranscripts.The
ornamentsareribosomalproteinsstartingto
associatewiththerRNAsaswellas
processingmachinery(snoRNPs),Some
branchesareshorterbecauseprocessingof
thosetranscriptshavealreadystarted.
MutationsI(Q)
100:Thetypeofmutationmostlikelyto
produceafunctionalprotein.
200:Thethreecategoriesofbase
substitutionmutations.
300:Stopcodons.
400:Giveanexampleofageneticdisease
causedbyamissenseorframeshiftmutation
affectingoneaminoacid.
500:Namefourtypesofmutations.
600:DNArepairsystemusingDNA
polymerases(only).
MutationsII(Q)
100:DNAPolymeraseIandIIIproofreadin
thisdirection.
200:HowdoestheDNArepairmachinery
distinguishbetweentheparentalDNA
strandsanddaughterDNAstrands?
300:ThemethylationsequencefoundinE.
coli.
400:ThethreeproteinsmentionedinMCB
150lecturethatareinvolvedinmismatch
repair.
500:Diseasesdiscussedinclassthatare
causedbyinsertionordeletionmutations.
600:DNArepairsystemthatusesDNA
polymeraseIII,DNAligase,andrepair
enzymes.
MutationsI(A)
100:Whatissamesenseorsilentmutation?
200:Whataresamesense,missense,and
nonsense?
300:WhatareUAA,UAG,UGA?
400:Whatis/aresicklecellanemia,PKU,or
cysticfibrosis?
500:Whatarebasesubstation,frameshift,
insertion,deletion,translocations,
duplications,andinversion?
600:Whatisproofreading?
MutationsII(A)
100:Whatis3-5?
200:Whatismethylatedvs.unmethylated
DNA?
300:Whatis5-GATC-3(andtheAdenine
getsmethylated)?
400:WhatareMutS,MutHandMutL(MutS
locatesthemismatch,MutHlooksfor
hemimethylatedDNAandholdsontothat
regionandcutsunmethylatedstrand,MutL
linksMutHandMutS)?
500:WhatareDuchenne'sMuscular
DystrophyandHaemophiliaA?
600:Whatismismatchrepair?
Nucleo-CytoTransport(Q)
100:Nuclearlocalizationsignalsarerichin
thistypeofaminoacid.
200:Nuclearexportsignalsarerichinthis
aminoacid.
300:Therangeinnanometersofanuclear
porecomplex.
400:Energyrequiredtorecycleimportin
andexportinisintheformofthismolecule.
500:Explainhowaproteincangetintothe
nucleus.(Youneedtousefourkeywordsto
gettheanswercorrect).
600:AproteintravelingfromtheGolgito
thelateendosomepresentsthissignal.
FinalJeopardy(Q)
Translatethefollowingportionofthe
templatestrandofDNAintoprotein.5-CGA
TGGCATACCGATCTG-3.
Figurebelowcorrespondsto”Domainsof
theNucleus”for500
Nucleo-CytoTransport(A)
100:Whatarebasicaminoacids?
200:Whatisleucine?
300:Whatis9nmto26nm?
400:WhatisGTP?
500:ProteinpresentsanNLS,Importin
recognizestheNLS,bindsandshowsasignal
tothenuclearporecomplex,Importinis
allowedin,Importinneedstoberecycled
usingGTP.
600:Whatismannose-6-phosphate?
FinalJeopardy(A)
Fliptemplatestrandsoitisread3to5.3-
GTCTAGCCATACGGTAGC-5:DNA.
TranscribemRNA5-CAGAUCGGUAUGCCA
UCG-3:mRNA.TranslatemRNA(5to3)N-
GlnIleGlyMetProSer-C:Protein
Figurebelowcorrespondsto“The
nucleolus”for600