INFLUENCE OF ALGAL PRODUCTION ON ECOSYSTEM METABOLISM, MICROBIAL
ACTIVITY AND NUTRIENT DYNAMICS IN A CENTRAL INDIANA STREAM
A THESIS SUBMITTED TO THE GRADUATE SCHOOL IN PARTIAL
FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE
MASTER OF SCIENCE
BY
ANDREA S. FITZGIBBON
ADVISORS: DR. MELODY J. BERNOT & DR. KEVIN H. WYATT
BALL STATE UNIVERSITY
MUNCIE, INDIANA
JULY 2014
INFLUENCE OF ALGAL PRODUCTION ON ECOSYSTEM METABOLISM, MICROBIAL
ACTIVITY AND NUTRIENT DYNAMICS IN A CENTRAL INDIANA STREAM
A THESIS SUBMITTED TO THE GRADUATE SCHOOL IN PARTIAL
FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE
MASTER OF SCIENCE
BY
ANDREA S. FITZGIBBON
ADVISORS: DR. MELODY J. BERNOT & DR. KEVIN H. WYATT
Committee Approval:
___________________________________________________________
Committee Chairperson
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Date
___________________________________________________________
Committee Member
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Date
___________________________________________________________
Committee Member
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Date
Departmental Approval:
___________________________________________________________
Departmental Chairperson
____________
Date
___________________________________________________________
Dean of Graduate School
____________
Date
BALL STATE UNIVERSITY
MUNCIE, INDIANA
JULY 2014
TABLE OF CONTENTS
TABLE OF CONTENTS…………………………………………………………………..…….iii
LIST OF FIGURES CHAPTER 1………………………………………………………...….…..iv
LIST OF TABLES CHAPTER 1………………………………………………………...….…...vi
LIST OF FIGURES CHAPTER 2……………………………………………………………….vii
LIST OF TABLES CHAPTER 2………………………………………………………………...ix
ABSTRACT……………………………………………………………………………………….1
ACKNOWLEGEMENTS…………………………………………………………………………3
CHAPTER 1: Comparison of algal and ecosystem metabolism and nutrient dynamics in a
central Indiana stream
ABSTRACT………………………………………………………………………………….……4
INTRODUCTION………………………………………………………………………….……..6
METHODS……………………………………………………………………………….……….8
RESULTS…………………………………………………………………………….………….14
DISCUSSION…………………………………………………………………….……………...21
CONCLUSIONS………………………………………………………………….…………......26
REFERENCES…………………………………………………………………….………….....27
FIGURES AND TABLES…………………………………………………………….………....32
CHAPTER 2: Influence of algal exudates on microbial activity and ecosystem dynamics
ABSTRACT………………………………………………………………………………….…..44
INTRODUCTION………………………………………………………………………….……46
METHODS……………………………………………………………………………….……...48
RESULTS…………………………………………………………………………….………….53
DISCUSSION…………………………………………………………………….……….……..56
CONCLUSIONS………………………………………………………………….…………......59
REFERENCES…………………………………………………………………….………….....61
FIGURES AND TABLES…………………………………………………………….………....66
APPENDIXES…………………………………………………………………………………...75
iii
LIST OF FIGURES
Figure
Page
CHAPTER 1: Comparison of algal and ecosystem metabolism and nutrient dynamics in
a central Indiana stream
1.
Conceptual model of hypothesized algal mediated diurnal patterns of nutrients, (top
panels) dissolved organic carbon (DOC), dissolve inorganic nitrogen (DIN);
physiochemistry, (middle panels) dissolved oxygen (O2) and pH; and metabolism,
(bottom panels) primary production, respiration and decomposition. ……………….….32
2.
Conceptual model of hypothesized seasonal water column and algae patterns of dissolved
oxygen (top panel); nutrients (middle panel); and metabolism, (bottom panels) primary
production and respiration. ……………………………………………………………..33
3.
Water column and algae diurnal variation in dissolved oxygen (DO, mg O2 /L)
concentration and pH over time in July and December 2013. ………………………….34
4.
Mean seasonal water column and algae dissolved oxygen concentrations. (n= 9 days per
season; Winter = Dec - Feb, Spring = Mar - May, Summer = Jun - Aug, Fall = Sep Nov). Error bars indicate SD. Dashed line indicates a 1:1 relationship. ………………..37
5.
Mean seasonal water column and algae pH. (n= 9 days per season; Winter = Dec - Feb,
Spring = Mar - May, Summer = Jun - Aug, Fall = Sep - Nov). Error bars indicate SD.
Dashed line indicates a 1:1 relationship. …………………………………………...…...38
6.
Mean seasonal water column and algae total organic carbon concentrations. (n = 9 days
per season; Winter = Dec - Feb, Spring = Mar - May, Summer = Jun - Aug, Fall = Sep Nov). Error bars indicate SD. Dashed line indicates a 1:1 relationship. …………….…39
7.
Mean seasonal water column and algae nitrate concentrations. (n= 9 days per season;
Winter = Dec - Feb, Spring = Mar - May, Summer = Jun - Aug, Fall = Sep - Nov). Error
bars indicate SD. Dashed line indicates a 1:1 relationship. ……………………………40
8.
Mean seasonal water column and algae phosphate concentrations. (n= 9 days per season;
Winter = Dec - Feb, Spring = Mar - May, Summer = Jun - Aug, Fall = Sep - Nov). Error
bars indicate SD. Dashed line indicates a 1:1 relationship. ……………………………41
iv
9.
Mean daily rates of (A) GPP, (B) ER and (C) NEP measured in White River, Yorktown,
Indiana from January through December 2013 (n = 2-3 days per month). Error bars
indicate SD. Shaded area (A) indicated mean dry algal biomass (n = 12). ……………42
v
LIST OF TABLES
Table
Page
CHAPTER 1: Comparison of algal and ecosystem metabolism and nutrient dynamics in
a central Indiana stream
1.
Physiochemical characteristics of the water column (Water) and algae in the White River,
Yorktown, Indiana from January through December 2013. Dissolved oxygen (DO), pH,
temperature and conductivity were measured continuously at 10 minute intervals over
approximately 72 hours (n = 125-432). Width (n = 1), flow and depth (n = 5) were
measured during the sampling event. Discharge (n = 1) was calculated from width, flow
and depth. Parentheses indicate standard error. …………………………………………35
2.
Cumulative mean water column (Water) and algae, total organic carbon (TOC), nitrate
(NO3), phosphate (PO4) and ammonium (NH4) concentrations. Phosphate concentrations
were the below detection limit (0.01 mg/L) September through December. Standard error
in parentheses, N= 48-144. ……………………………………………………………...36
3.
Multiple regression model covariates for water and algal gross primary production (GPP)
and ecosystem respiration (ER). ……………………………………………....….……..43
vi
.
LIST OF FIGURES
Figure
Page
CHAPTER 2: Influence of algal exudates and benthic substrata on microbial activity
1.
Mean dissolved oxygen across mesocosm treatments (algae, exudate and control) over
three sampling events (n = 126 for algae treatment, n = 63 for exudate and control
treatments). Error bars indicate SE. Note y-axis begins at 7.00 mg O2/ L. Dissolved
oxygen differs by treatment (p = 0.027) but not by substrate (p= 0.241) with no
interaction between main effects (p = 0.723). Pairwise differences indicated by letters
above bars. ………………………………………………………………………………66
2.
Mean pH of mesocosm (A) treatment (algae, exudate and control) and (B) substrate
(sediment, leaves, sponge and glass) over three sampling events (n = 126 for algae
treatment, n = 63 for exudate and control treatments, n = 60 for each substrate). Error
bars indicate SE. Note y-axis begins at 8.00. pH differs by treatment and substrate (p <
0.001) with no interaction between main effects (p = 0.397). Pairwise differences
indicated by letters above bars. ………………………………………………………….68
3.
Mean total organic carbon (TOC) of mesocosm (A) treatment (algae, exudate and
control) and (B) substrate (sediment, leaves, sponge and glass) over two sampling events
(n = 32 for algae treatment, n = 16 for exudate and control treatments, n = 16 for each
substrate). Error bars indicate SE. TOC differs by treatment (p < 0.0001) and substrate (p
< 0.0001) with an interaction between main effects (p = 0.0073). Pairwise differences
indicated by letters above bars. ……………………………………………………….…69
4.
Mean nitrate (NO3) concentration by mesocosm (A) treatment (algae, exudate and
control) and (B) substrate (sediment, leaves, sponge and glass) over two sampling events
(n = 64 for algae treatment, n = 32 for exudate and control treatments, n = 32 for each
substrate). Error bars indicate SE. NO3 differs by treatment (p = 0.0002) and substrate (p
< 0.0001) with an interaction between main effects (p = 0.0003). Pairwise differences
indicated by letters above bars. …………………………………………………………70
5.
Mean phosphate (PO4) concentration by mesocosm (A) treatment (algae, exudate and
control) and (B) substrate (sediment, leaves, sponge and glass) over two sampling events
(n = 64 for algae treatment, n = 32 for exudate and control treatments, n = 32 for each
substrate). Error bars indicate SE. PO4 differs by treatment (p = 0.0003) and substrate (p
vii
6.
= 0.0003) with no interaction between main effects (p < 0.0001). Pairwise differences
indicated by letters above bars. …………………………………………………………71
Nutrient ratios of total organic carbon (TOC) to nitrate (NO3) and phosphate (PO4)
among treatments (algae, exudate and control). Error bars indicate SE. TOC: NO3 did not
differ by treatment (p = 0.263) nor did TOC: PO4 (p = 0.126). Dashed lines indicate
differences in nutrient availability; arrows indicate high or low amounts of nutrient
available. ………………………………………………………………………………...72
7.
Mean respiration by mesocosm substrate type (sediment, leaves, sponge and glass; n =
20). Error bars indicate SE. Respiration differs by substrate (p < 0.001) but not by
treatment (p = 0.997) with no interaction between main effects (p = 0.803). Pairwise
differences indicated by letter above bars. ……………………………………………....73
8.
Relationship between total organic carbon (TOC) concentration and microbial respiration
by treatment (algae, exudate and control) (p < 0.01). ………………………………..….74
viii
LIST OF TABLES
Table
Page
CHAPTER 2: Influence of algal exudates and benthic substrata on microbial activity
1.
Mean mesocosm temperature (°C) across algal and substrate treatments over three
sampling events (n = 126 for algae treatment, n = 63 for exudate and control treatments
and n = 60 for substrate treatments). Numbers in parentheses are SE. Temperature differs
by treatment (p = 0.010) but not by substrate (p = 0.573) with no interaction between
main effects (p = 0.371). Pairwise difference denoted by letters. ………………………67
ix
ABSTRACT
THESIS: Influence of algal production on ecosystem metabolism, microbial activity and nutrient
dynamics in a central Indiana stream
STUDENT: Andrea S. Fitzgibbon
DEGREE: Masters of Science
COLLEGE: Sciences and Humanities
DATE: July 2014
PAGES: 155
One primary function in most ecosystems is photosynthesis which provides energy for
consumers. In mid-order streams, benthic algae are primary producers fulfilling this role and
their influence on stream biogeochemistry changes with the diurnal patterns of production.
During the day, photosynthesis increases stream oxygen concentrations facilitating shifts in pH
and nutrient concentrations. However, little is known about diurnal patterns of nutrient
concentrations. Algal production regulates nutrient cycling through assimilation of inorganic
nutrients and release of nitrogen and carbon exudates. Though nutrient assimilation by algae in
streams is well understood, understanding of algal nutrient release is limited. Nutrient release by
algae is a significant gap in our knowledge of ecosystem dynamics, especially in nutrient
saturated streams. To understand algal dynamics in a nutrient saturated stream, algal production
in a mid-order stream was measured via monthly sampling for one year using microelectrodes to
quantify algal biofilm chemical gradients of dissolved oxygen and pH. Ecosystem energetics as
gross primary production (GPP) and ecosystem respiration (ER) were also calculated. Nutrients
were analyzed from water column and algal pore water samples. Dissolved oxygen
concentrations and pH between the algae and water column followed similar diurnal curves
although the amplitude of these changes varied seasonally. Nutrient and total organic carbon
1
concentrations varied seasonally. Overall, algae contributed ~ 13 % of primary production to
total ecosystem production. Additionally, we assessed the influence of algal production on
microbial activity using an in vitro factorial experiment. The objective of the experiment was to
understand how algae may stimulate microbial respiration in a eutrophic stream ecosystem. An
algal effect on microbial respiration after 3 days, was not observed likely because the labile algal
exudates were quickly assimilated before the assay was conducted. Leaf substrates yielded the
highest rates of microbial respiration likely due to higher carbon availability associated with
leachate. Continued study of microbial responses to algae and benthic substrates will help predict
ecosystem responses with changing nutrient status as streams become eutrophic.
2
ACKNOWLEDGEMENTS
I would first like to thank the funding sources for this study including Ball State Graduate
Student Research Program, Ball State University ASPiRE Graduate Research Program and the
Indiana Academy of Science Welch Botanical Award. Thanks to Adam Carpenter, Ron and Toni
Fitzgibbon, Jessica Moak and Ethan Toombs for their field assistance as well as Susannah
Dragash and James Justice for their laboratory assistance. Thanks to Dr. Allison Rober for her
time spent serving as a valuable committee member for this project. Lastly, I thank my advisors,
Dr. Melody Bernot and Dr. Kevin Wyatt for the opportunity to be co-advised which has
developed my research skill and interests, for their time, expertise and for their valuable
guidance and advice throughout this study.
3
CHAPTER 1: Comparison of algal and ecosystem metabolism and nutrient dynamics in a
central Indiana stream
Abstract
Benthic algae are primary producers in streams and can regulate nutrient cycling through
assimilation and release of nutrients which may influence ecosystem process rates. Algal biofilm
and water column metabolism estimates were quantified monthly over 1 yr in a central Indiana
3rd order stream. Biofilm dissolved oxygen (DO) and pH were measured continuously using
microelectrodes and contrasted with water column dynamics. Specifically, algal DO
concentrations ranged from 2.41 - 16.95 mg L-1 while water column DO concentrations ranged
from 5.07 - 18.07 mg L-1. Algal pH (7.75 - 8.65) was lower than water column pH (8.88 - 9.73).
Algae and the water column DO concentrations and pH elicited similar diurnal patterns that
varied seasonally. Overall, algal pore water had higher total organic carbon concentrations
relative to the water column. Nitrate concentrations were comparable between the algal pore
water and water column in the winter and fall but algal pore nitrate concentrations were lower in
the spring and higher in the summer relative to the water column. Phosphate concentrations were
comparable between the algal pore water and water column during all seasons and phosphate
was below detection in the fall and winter. Algal gross primary production (GPP; 0.31 g O2 m-2
d-1) and ecosystem respiration (ER; 0.31 g O2 m-2 d-1) was ~10x lower than water column GPP
(3.22 g O2 m-2 d-1) and ER (3.21 g O2/m-2 d-1). Algae and water column NEP varied seasonally
and reflected the differential spatial scales as the microbial community was more closely
associated with algae. Overall algal GPP contributed ~ 13 % of total ecosystem production,
though multiple regressions did not show that algal GPP or ER contributed to water column
GPP. Although algal production did not directly influence water column metabolism, algal
4
biomass and algal dissolved oxygen as well as total organic carbon concentrations did influence
water column metabolism. These data provide insight on algal production influence on
ecosystem process rates via physiochemical changes in the environment. Further, these findings
show that even in nutrient saturated systems, algae influence the water column through changes
in physiochemical changes.
5
Introduction
Primary production and respiration are metrics used to quantify metabolism (Odum
1956) to indicate biotic activity which varies based on community structure (Cummins 1974).
Biotic communities are also influenced by surrounding landscape which can contribute
allochthonous or autochthonous substrates for metabolism which can affect downstream
ecosystem processes (Vannote et al. 1980, Odum and Barrett 2005). Allochthonous inputs are
associated with small order and larger order streams whereas autochthonous inputs are generally
associated with middle order streams. Small order streams typically yield low primary
production as riparian vegetation restricts solar radiation available for photosynthesis resulting in
a net heterotrophic ecosystem (Hill et al. 2001). Leaf litter also provides a carbon substrate for
bacteria and fungi to contribute to respiration in smaller order streams (Petersen and Cummins
1974, Hieber and Gessner 2002). In larger order streams, increased depth and allochthonous
inputs of accumulated materials from upper reaches (i.e., sediments) reduce light penetration and
benthic substrate which supports respiration rates that can exceed primary production (Vannote
et al. 1980, Chapin et al. 2002). Metabolism shifts from a net heterotrophic ecosystem in small
and larger order systems towards a net autotrophic ecosystem in middle order reaches. Less
canopy cover allows for increased light and primary production providing autochthonous sources
of carbon into the stream (Webster et al. 1995).
Benthic algae are dominant primary producers in middle order streams (Stevenson et al.
1996) and their influence on stream biogeochemistry changes with the diurnal patterns of
production (Odum 1956, Roberts et al. 2007). During the day, photosynthesis increases stream
dissolved oxygen concentrations (Mulholland et al. 2005) as well as pH (Talling 1976, Revsbech
et al. 1983) and nutrient concentrations (Fig. 1; Kaplan and Bott 1982, Johnson and Tank 2009)
6
which all decrease to baseline levels at night. Algal production regulates nutrient cycling through
assimilation of inorganic nutrients (removal from the water column) and release of nitrogen and
carbon exudates (loss to the water column). Though nutrient assimilation by algae in streams is
well understood (Bertilsson and Grace 2003), understanding of the factors influencing algal
nutrient release is limited. Algal nutrient release represents a significant gap in our understanding
of ecosystem dynamics, especially in nutrient saturated streams (Harbott and Grace 2005,
Wiegner et al. 2005).
Streams are becoming increasingly eutrophic with anthropogenic contaminants from
agriculture and urbanization (Carpenter et al. 1998, Lewis et al. 2011) which can increase algal
production (Blum 1956, Francoeur 2001), biomass (Stevenson 1997) and subsequently alter
ecosystem processes and trophic interactions (Power 1992). Therefore, understanding how algae
influence biogeochemistry can provide insight in predicting future responses in terms of
heterotrophic metabolism, nutrient cycling and food web dynamics with anticipated changes in
the environment.
The goal of this research was to understand how algal production influenced algal and
ecosystem metabolism in a mid-order stream through diurnal and seasonal changes in the
physiochemical environment. Research was guided by specific questions and related hypotheses
including: 1) How does algal production influence diurnal and seasonal patterns of oxygen and
pH within an algal biofilm relative to the water column?; and, 2) How does algal production
influence nutrient concentrations within algal pore water relative to the water column? We
hypothesized that diurnal dissolved oxygen (DO) and pH within the algal biofilm would increase
(Fig.1) with algal production and algal biofilm patterns in DO and pH would be consistent with
water column patterns. We hypothesized that seasonal algal and water column DO patterns
7
would not be similar (Fig. 2) as algal production increased during the summer and decreased
during the winter. We further hypothesized that as algal production increased with sunlight, algal
pore water nutrient concentrations would also increase (Fig. 1) due to exudate release and this
would not be consistent with nutrient concentrations in the water column as labile exudates
released during photosynthesis would be quickly assimilated. We hypothesized that algal and
water column nutrient concentrations would differ in the spring and summer but be comparable
in fall and winter (Fig. 2). Specifically, we hypothesized that algal pore water concentrations
would decrease relative to the water column in the spring with increased assimilation and algal
pore water would increase relative to the water column in the summer with algal senescence
releasing exudates.
Methods
Study Site
Sampling was conducted in the White River, a 3rd order stream in Yorktown, Indiana,
part of the Upper White River Watershed. The White River at Yorktown was selected for the
presence of filamentous green algae attached to cobbles on the benthos throughout the year.
Agricultural and urban activities including combine sewer overflows (CSOs) have contributed to
high nitrogen concentrations within this sub-watershed (UWRWA 2011). As of 2011, this subwatershed was composed of 51.8% agriculture, 25.4% herbaceous, 15.0% urban, 5.2% forest,
1.9% wetland and 0.8% open water (UWRWA 2011).
Field Sampling
The White River was sampled monthly over 72 h events for one year (n = 12 sampling
events: January 2013 – December 2013). During each event, algae and water column
8
physiochemical characteristics were recorded at 10 minute intervals using microelectrodes
inserted into a filamentous green algae mat and a Hydrolab minisonde placed in a well-mixed
portion of the water column. Water column and algal pore water samples were also collected
every 3 h (except between 0000 and 0600) for measurement of nutrient concentrations (n = 6
water column and pore water samples per 24 h event). Stream width (m), depth (m), velocity
(m/s), and discharge (m3/s) were also measured monthly.
The width (m) of the stream was measured using metric tape followed by measurement of
velocity using a Marsh-McBirney flow meter at 5 equidistant points across the width to measure
depth (m) and velocity (m/s). Discharge (m3/s) was estimated by multiplying the width (m),
mean depth (m) and mean velocity (m/s).
Benthic algal biomass was collected using a 5.08 cm diameter PVC cylinder to isolate a
known area (0.002 m2) and the algae within that area was scraped off of the cobble and placed
into an individual container with 50 mL of stream water. Five samples per month were collected
from five separate cobbles covered with filamentous green algae mats. Samples were placed on
ice in the dark for transport and then placed in a drying oven (60˚C) and subsequently weighed
(Stevenson and Bahls 1999).
For benthic substrate characterization, the stream reach was divided into a 10 x 10
transect scaled by the width of the stream so there were 10 equidistant transects along the width
and length of the stream which yielded 100 quadrants to describe the substrate (leaf, cobble or
algae) present on the benthos. The percent substrate cover was calculated as:
Substrate Cover (%)
100
of
adrants ith s
100
Algae Physiochemical Measurements
9
strate (leaf co
adrants
le or algae)
Measurements of in situ algae pH and dissolved oxygen were conducted using Clark-type
microelectrodes (OX –N and pH –N, Unisense, Aarhus N, Denmark) connected to an
UnderWater Meter system with external battery pack for 72 h of continuous data collection at 10
minute intervals. Microelectrodes were calibrated 1-3 d prior to and following each field
sampling event. Oxygen microelectrodes were calibrated using a 6-point calibration curve
derived from microelectrode response (mV) paired with known oxygen concentrations (mg O2 L1
) from a hand-held dissolved oxygen meter (YSI, DO200). Oxygen concentrations were
manipulated with O2 and N2 bubbling in tap water ranging from approximately 3 - 16 mg O2 L-1.
Buffer solutions (4.00, 7.00 and 10.00) were used to calibrate the pH microelectrode; the mV
response was compared to a pH measured using a hand-held meter (Extech, pH200). Calibration
curves were created using linear regressions to represent the relationship between the
microelectrode response and the known concentration or unit. All linear regressions had an r2 of
0.85 or above.
During each sampling event, microelectrodes were positioned in the stream over a
filamentous green algae mat attached to cobble in the benthos. Two dissolved oxygen and one
pH microelectrode were suspended at approximately the same level using a custom four-way
microelectrode clamp attached to a Unisense micromanipulator stand which was placed
downstream in relation to the algal mat. The microelectrodes were inserted approximately 5 mm
into the interior of the algal mat and programmed to log every 10 minutes for 72 h.
Microelectrodes were checked every ~3 h to remove any debris (e.g., leaves, sticks,
macrophytes). Microelectrode data were collected in mV and subsequently converted to mg O2
L-1 and pH units using the combined pre- and post- calibration curves.
10
Water Column Physiochemical Measurements
A Hydrolab minisonde was deployed in a well-mixed portion of the water column ~10 m
upstream from the microelectrodes in a region with no visible algal mats. The Hydrolab
minisonde was equipped with a Luminescent Dissolved Oxygen (LDO) sensor for measurement
of water column dissolved oxygen (mg L-1) temperat re (˚C) specific cond ctivity (μS/cm) and
pH every 10 minutes for the 72 h duration. Dissolved oxygen concentration was calibrated
according to manufacturer instructions prior to each sampling event.
Metabolism Calculations
Algae and water column metabolism including gross primary production (GPP),
ecosystem respiration (ER) and net ecosystem production (NEP) were calculated using the
nighttime regression method (Owens 1974, Hornberger and Kelly 1975, Young and Huryn
1999). Diurnal oxygen curves (24 h) for the algae and water column were selected for each
sample month (n = 1 - 3) for a total of 65 diurnal curves. Temperature data coupled with the
selected dissolved oxygen diurnal period was used to determine dissolved oxygen saturation.
Since temperature was only logged in the water column it was also used in calculating the
dissolved oxygen saturation for the algae. Before quantifying metabolism in the water column
and algae, a reaeration coefficient
was calculated to adjust for oxygen diffusion. To quantify
K, raw data were used to quantify changes from saturation and change in dissolved oxygen
concentration over time as described below. Dissolved oxygen concentration at saturation ( )
was calculated based on temperature and barometric pressure. Change in the concentration of
dissolved oxygen over 10 minutes was determined(
). Change in measured dissolved
oxygen concentration from the calculated dissolved oxygen concentration at saturation was
11
calculated for each interval(
). To determine the rate of change in dissolved oxygen, the
difference in concentration (
) was divided by the 10 min interval
). This rate of change ( ) was plotted against the dissolved oxygen
period(
concentration change from saturation (
) during nighttime hours only which were
considered sunset to sunrise. A linear regression was used to represent the relationship between
the rate of dissolved oxygen change and deviation from saturation. The slope of the linear
regression equation served as the reaeration coefficient (K). The reaeration coefficient was then
incorporated into equations to quantify NEP, GPP and ER for the algae and water column each
month.
NEP, mg O2 L-1
(
(
))
GPP, mg O2 L-1
[
(
)]
[((
)
(
))( )]
ER, mg O2 L-1
Units for these calculations were adjusted so the metabolism estimates were expressed as g O2 m2
d-1. The change in units incorporated the depth of the algae or water column in meters. The
depth of the algae was estimated when adjusting the microelectrode depth in the interior of the
algal mat. The depth of the algal mat varied temporally (average depth = 0.02 m).
Nutrient Concentrations
Water samples for nutrient analyses from both the water column and algal pore water
were collected every 3 h during the 72 h sampling event. All water samples collected were
filtered on site using a syringe filter containing a 25 mm Whatman glass fiber filter (GF/F; 0.7
micron pore size) in 20 mL high density polyethylene (HDPE) scintillation vials. All samples for
12
total organic carbon analysis were immediately acidified with 2 drops of 1.0 N HCl to inhibit
microbial activity and convert all inorganic forms of carbon to carbon dioxide gas. All samples
were then placed on ice and frozen for subsequent analyses.
Algae pore water samples were collected using individual 5 mL syringes. Each 5 mL
sample was collected directly above the surface of the algae to collect the biofilm pore water and
each sample came from a different algal mat. Three 5 mL syringes were then filtered into each
scintillation vial for a composite sample totaling 15 mL and 8 vials total. Four composite
scintillation vials were used for nutrient analyses and four composite scintillation vials were
acidified for total organic carbon analysis.
Water column samples were collected using four 60 mL syringes at equidistant points
across the width of the stream. Each 60 mL syringe was divided among two scintillation vials
resulting in eight 20 mL scintillation vials. Four scintillation vials were used for nutrient analyses
and four scintillation vials were acidified for total organic carbon analysis.
Nutrient Analysis
Nitrate (NO3) and phosphate (PO4) concentrations were measured for all samples with
analytical duplicates on a Dionex ion chromatograph (Dionex Corporation, Sunnyvale,
California) using standard methods (Eaton et al. 2005). Detection limits were 0.01 mg L-1 NO3
and 0.01 mg L-1 PO4. Ion concentrations below detection were reported as zero. Total organic
carbon concentrations were measured for all samples with a Shimadzu TOC-L analyzer
(Shimadzu Scientific Instruments, Columbia Maryland) using standard methods (Eaton et al.
2005). Water column samples were analyzed using analytical duplicates. However, because of
13
the large volume (40 mL) needed for TOC analysis, algal pore water samples were analyzed as
composite samples for each time point.
Ammonium (NH4) concentrations were quantified using the phenylhypochlorite
spectrophotometric technique on selected water column and algal pore water samples
representative of each season (April, July, October and December; APHA 1995, Aminot et al.
1997). Samples were analyzed on a spectrophotometer (UV-1700 PharmaSpec, Shimadzu) at
650 nm. The a sor ance val es ere then converted to μg NH4 L-1 using a standard curve of
ammonium chloride (NH4Cl) absorbance. The NH4 detection limit as 0.1 μg L-1 NH4.
Statistics
Independent variables for one-way analysis of variance (ANOVA) and paired t-tests were
used for assessing differences in net ecosystem production (NEP), gross primary production
(GPP), ecosystem respiration (ER), total organic carbon (TOC), nitrate (NO3), phosphate (PO4),
ammonium (NH4), dissolved oxygen (DO) and pH among months and between the water column
and algae. In addition to these independent variables, Pearson correlation statistics also
incorporated temperature, conductivity, discharge, flow, width, water column depth, algae depth
and algal biomass to identify related factors. Multiple regression analyses were also used to
identify the independent variables that influenced variation of GPP and ER from the water
column and algae individually. All statistical tests were performed with Minitab 16.2.4.
Results
Algae and water column diurnal variation
14
Dissolved oxygen and pH varied diurnally in both the algae and water column (Fig. 3)
and the amplitude of the measurement varied seasonally. July DO concentrations in the algae
(range = 3.54 – 15.88 mg L-1) and water column (range = 4.74 – 9.97 mg L-1) were comparable
at night but algal primary production increased the DO concentration during the day to ~ 6 mg L1
higher than the water column DO concentration. In contrast, water column DO concentrations
peaked during the day and were consistently 9 – 12 mg L-1 higher than the algal DO
concentrations in December. July pH in the algae (range = 7.70 – 8.30) and water column (range
= 8.89 – 9.25) as well as December pH in the algae (range = 7.87 – 8.71) and water column
(range = 9.38 – 9.77) differed by ~ 1 pH unit and followed the diurnal variation of DO. Overall,
pH was highest during the day and lowest during the night.
Physical characteristics
Temperature varied temporally (Table 1) with the lowest temperature in the winter
months (Decem er mean = 1.37 ˚C) and the highest temperat re the s mmer months (J ne mean
= 23.19 ˚C). Cond ctivity as lo est in the inter and early spring (March 448.20 μS cm-1) and
highest in the fall (Octo er 1225.40 μS cm-1). Algal depth varied from 0.015 m to 0.03 m with a
mean depth of 0.02 m. Algal biomass was highest in March (214.1 gdm m-2) and lowest in June
(26.2 gdm m-2) following a flood event. The width of the stream was greatest (21 m) from
January to April and decreased in the spring to ~17 m through the summer, decreasing into fall
(~15 m). Flow was lowest in the late summer and fall (0.27 – 0.38 m s-1) and highest in in the
late winter and spring (0.70 – 1.03 m s-1). Discharge was calculated from the depth, width and
flow (range = 1,190 – 12,220 L s-1).
15
Flow correlated positively with depth (R = 0.76, p < 0.01). However, flow negatively
correlated with algal depth (R = -0.74, p < 0.01). Temperature negatively correlated with stream
depth (R = -0.62, p = 0.03). Algal biomass negatively correlated with temperature (R = -0.582, p
= 0.05).
Algal dynamics
Algal dissolved oxygen (DO) concentration varied seasonally (p < 0.001; range = 4.73 –
9.57 mg L-1; Table 1). Dissolved oxygen concentrations in the algae decreased in the fall
beginning in September (mean = 6.02 mg L-1) with the lowest concentration measured in
December (min = 3.00 mg L-1). Dissolved oxygen concentrations also increased in the spring
with a shift from February (mean = 6.26 mg L-1) to March (mean = 8.05 mg L-1). Algal pH
(Table 1) ranged from 7.75 – 8.65 being lowest from May to September (mean = 7.99) and
highest from October to April (mean = 8.49).
Algal pore water TOC concentrations did not vary seasonally (p = 0.09; range = 4.26 –
7.33 mg L-1; Table 2). Algal pore water TOC concentrations was highest in March and April
(mean = 7.26 – 7.33 mg L-1). In contrast, algal pore water NO3 concentrations did vary
seasonally (p < 0.01; 5.02 – 75.95 mg L-1; Table 2). Specifically, algal pore water NO3
concentrations were highest in June (mean = 75.95 mg L-1) and July (mean = 73.19 mg L-1)
relative to winter measurements (mean = 5.02 mg L-1). Algal pore water PO4 concentrations
varied seasonally (p < 0.01; range= BDL – 29.30 mg L-1; Table 2) being higher in the summer
(range = 7.87 – 29.30 mg L-1) relative to fall and winter measurements (range = BDL – 1.34 mg
L-1). In contrast, algal pore water NH4 concentrations did not vary seasonally (p > 0.05; range =
4.20 – 5.22 μg L-1; Table 2).
16
Water column dynamics
Water column DO concentrations varied seasonally (p < 0.001; range = 6.78 – 15.5 mg L1
; Table 1) with the lowest concentrations in August and highest concentrations in December.
Water column pH also varied seasonally (p < 0.001; range = 8.88 – 9.73; Table 1). Specifically,
water column pH was lowest from May to November (mean = 9.09) and was highest from
December to April (mean = 9.50 mg L-1).
Water column TOC concentrations (p < 0.01; range = 2.89 – 8.38 mg L-1; Table 2) were
highest in June following a storm event and lowest in January. Water column NO3
concentrations also varied seasonally (p < 0.01; range = 4.59 – 90.14 mg L-1; Table 2)being
highest in March (mean = 90.14 mg L-1) and June (mean = 76.14 mg L-1) relative to December
measurements (mean = 4.59 mg L-1). Water column PO4 concentrations were highest in the
summer (range = 8.34 – 30.18 mg L-1) relative to fall and winter measurements (range = BDL –
1.79 L-1) (p < 0.01; range = BDL – 30.18 mg L-1; Table 2). In contrast, water column NH4
concentrations did not vary seasonally (p = 0.08; range = 2.44 – 5.03 μg L-1; Table 2).
Relationships between the algae and water column
Algae and water column DO concentrations were negatively correlated (R = -0.398, p =
0.20; Fig. 4) whereas algae and water column pH were positively correlated (R = 0.696, p =
0.01; Fig. 5). There was a seasonal shift in the relationship between water column and algal DO
concentrations. Specifically, water column DO concentrations were highest in the winter when
algal DO concentrations were low (p < 0.01). This relationship shifted in the spring as algal DO
concentrations increased and water column DO concentrations decreased, likely associated with
17
primary production and increasing temperatures, respectively. Algal DO concentrations were
significantly lower than water column DO concentrations from October – May (p = 0.09) while
algal DO concentrations were comparable to water column DO concentrations for June –
September (p = 0.61). In contrast, algal pH was significantly different from water column pH
across all months (p < 0.01).
As water column DO concentrations increased, temperature decreased (R = -0.90, p <
0.01). Water column DO concentrations increased with algal biomass (R= 0.667, p = 0.02). In
contrast, there was not a relationship between algal DO concentrations and algal biomass (R = 0.200, p = 0.532). As water column pH (R = -0.863, p < 0.01) and algal mat pH (R = -0.758, p <
0.01) increased, temperature decreased. Water column DO increased with water column pH (R =
0.841. p < 0.01).
Across sampling events, there was no difference between algal pore water and water
column PO4 concentrations (p > 0.05; Fig. 8). However, algal pore water NO3 concentrations
were 16 – 26 % higher in July and August relative to the water column (Fig. 7). Additionally,
algal pore water TOC concentrations were 20 – 60 % higher than water column TOC
concentrations (Fig. 6) from January through April (p = 0.003 – 0.059) and in October (p =
0.04). While algal pore water and water column NH4 concentrations were comparable in April,
July and October (p > 0.05), in December algal pore water NH4 was 49 % higher than water
column NH4 concentrations (p = 0.02).
As water column TOC concentrations increased, temperature also increased (R = 0.570, p
= 0.05).and water column DO concentrations decreased (R = -0.548, p = 0.07). Water column
NO3 concentrations increased with water column NH4 (R = 0.951, p = 0.05). Additionally, algal
pore water NO3 concentrations increased with water column NH4 concentrations (R = 0.946, p =
18
0.05) as well as water column NO3 concentrations (R = 0.870, p < 0.01). Water column PO4
concentrations increased with temperature (R = 0.671, p = 0.02) and decreased with stream depth
(R = -0.678, p = 0.02), consistent with algal pore water PO4 concentrations.
Algal and ecosystem metabolism
Algal gross primary production (GPP) varied seasonally (p < 0.01; range = 0.005 – 2.16 g
O2 m-2 d-1; Fig. 9a). Algal GPP was highest in August and lowest in January. Water column GPP
also varied seasonally (p = 0.01; range = 1.27 – 6.15 g O2 m-2 d-1; Fig. 9a) being highest in June
and lowest in January. Algal and water column GPP were similar in January, March, and June (p
> 0.05) but differed in February, April, May, and July through December (p = 0.01 – 0.03).
Water column GPP was consistently 101 – 198 % greater than algal GPP except in August when
water column GPP was only 10 % greater than algal GPP. While water column GPP was
consistently higher than algal GPP, the contribution by algae to the ecosystem production varied
seasonally. Relative to water column GPP, algae contributed the lowest amount of GPP in the
winter (< 1 %) and the highest amount in the summer (41 %). Overall, algal GPP contributed
~13 % of production to the water column GPP.
Algal ecosystem respiration (ER) varied seasonally (p < 0.01; range = 0.02 – 2.17 g O2
m-2 d-1; Fig. 9b). Algal ER was highest in August and lowest in February. Water column ER also
varied seasonally (p < 0.01; range = 1.09 – 5.71 g O2 m-2 d-1; Fig. 9b) being highest in April and
lowest in January. Algal and water column ER were similar in March and June (p > 0.05) but
differed in January, February, April, May and July through December (p > 0.01 – 0.05). Water
column ER was consistently 108 – 196 % greater than algal ER except in August when water
column ER was only 14 % greater than algal ER. While water column ER was consistently
19
higher than algal ER, the contribution by algae to the ecosystem varied seasonally. Relative to
water column ER, algae contributed the smallest amount of ER in the winter (~ 1 %) and the
highest amount in the summer (39 %).
Algal NEP was highest in March and lowest in August (p < 0.01; range = -0.02 – 0.09 g
O2 m-2 d-1; Fig. 9c). Water column NEP also varied seasonally (p < 0.01; range = -0.36 – 0.86 g
O2 m-2 d-1; Fig. 9c) and was highest in June and lowest in October. Algal and water column NEP
differed in March, July, October and November (p = 0.01 – 0.03).
Algal GPP increased with algal ER (R = 0.999, p < 0.01). Algal mat GPP (R = 0.677, p =
0.02) and algal mat ER (R = 0.665, p = 0.02) both increased with algal DO concentrations. Algal
GPP (R = 0.534, p = 0.07) and algal ER (R = 0.541, p = 0.07) increased with water column PO4.
Water column GPP increased with water column (R = 0.988, p < 0.01). Water column GPP (R =
0.813, p < 0.01) and water column ER (R = 0.821, p < 0.01) increased with water column TOC.
Water column GPP (R = 0.493, p = 0.10) and water column ER (R = 0.526, p = 0.08) also
increased with algal TOC concentrations. Water column GPP (R = -0.987, p < 0.01) and water
column ER (R = -0.970, p = 0.03) increased with decreased in water column NH4 concentrations.
Water column GPP was influenced by water column ER, algal biomass, algal and water
column DO concentrations and water column pH (R2 = 99.87, S = 0.0834). Several covariates
were consistent with the correlation analysis including water column ER and water column DO
concentrations. Water column pH did not correlate directly with water column GPP but it did
with water column DO concentrations. Algal biomass also helped explain some of the variation
which suggested that it had an indirect effect as it correlated with water column DO
concentrations which also influenced water column GPP. Water column ER was influenced by
water column GPP, algal and water column TOC concentrations (R2 = 99.12, S = 0.1690). These
20
covariates were consistent with correlation analyses. Algal GPP was influenced by algal ER and
algal biomass (R2 = 99.88, S = 0.0258). Algal ER was influenced by algal GPP and algal
biomass (R2 = 99.85, S = 0.0255). Algal biomass did not correlate directly with algal GPP or ER
but suggested an indirect effect.
Discussion
These findings provide an account of the influence of algal production on stream
physiochemical characteristics, nutrient concentrations, and metabolism at diurnal and seasonal
scales in a eutrophic stream. The study site selected was representative of one of the most
degraded watersheds in the United States (UWRWA 2011) and the results should be considered
in the context of a high nutrient system. In this nutrient saturated system, algal production
influenced water column physiochemical variation and contributed only a small portion (~ 13 %)
of total ecosystem production with the highest contribution in the summer. This contrasted the
idea that open-canopy systems have primary production contributions to total ecosystem
production > 50 % (Minshall 1978) in undisturbed streams. Therefore nutrient saturated systems
may limit the influence primary producers have on total ecosystem production but the
autochthonous sources are detectable even with large contributions from allochthonous sources.
Alternatively, other primary producers (e.g., macrophytes) may contribute a significant amount
of production in some ecosystems.
Factors influencing algae and water column metabolism
Although algal metabolism (GPP and ER) did not directly influence water column
metabolism components, algal biomass, DO and TOC concentrations (mediated by algal
21
metabolism) did influence water column metabolism estimates. Algal biomass and DO
concentrations influenced water column GPP while algal TOC concentrations influenced water
column ER. Algal biomass was also found to be an important driver of ecosystem GPP in a
multi-regional study (Bernot et al. 2010) and in a forested headwater stream (Roberts et al.
2007). In contrast to our study, algal dissolved oxygen concentrations have not been related to
ecosystem metabolism in the literature. Perhaps this is because diurnal dissolved oxygen
concentrations from algal biofilms are not typically collected and therefore not incorporated into
regression models. If algal DO concentrations were exempt from the model, in this study PO4
concentrations from both the algae and water column in addition to biomass and water column
TOC concentrations would explain 96 % of the variation in water column GPP. This is consistent
with a multi-regional study (Mulholland et al 2001) where PO4 concentrations influenced water
column GPP. Water column ER was driven by algal and water column TOC which was
consistent with another multi-regional study (Bernot et al 2010) where these covariates explained
variation in water column ER.
Estimates of water column GPP and ER in the current study (GPP: 1.27 – 6.15 g O2 m-2
d-1, mean = 3.22 g O2 m-2 d-1; ER: 1.09 – 5.17 g O2 m-2 d-1, mean = 3.21 g O2 m-2 d-1) and algal
GPP and ER (GPP: 0.005 – 2.16 g O2 m-2 d-1, mean = 0.31 g O2 m-2 d-1; ER: 0.02 – 2.17 g O2 m-2
d-1, mean = 0.31 g O2 m-2 d-1) are comparable to values reported in a variety of studies with
different land uses. Water column metabolism estimates were similar to values reported with
catchments that included pastures (GPP: 1.26 -3.68 g O2 m-2 d-1; ER: 2.02 – 2.68 g O2 m-2 d-1,
Young and Huryn 1999), agriculture (mean GPP: ~ 4 g O2 m-2 d-1; mean ER: ~6 g O2 m-2 d-1,
Bernot et al. 2010) and urbanization (mean GPP: 3.5 g O2 m-2 d-1; mean ER: 4.2 g O2 m-2 d-1,
Pennino et al. 2014). Water column and algal metabolism estimates also both fell within the
22
bounds of estimates from an agriculturally influenced stream (GPP: 0.1 – 6.2 g O2 m-2 d-1; ER:
0.05 – 6.8 g O2 m-2 d-1, Frankforter et al. 2010).
Our water column metabolism estimates were different compared to a low nutrient,
geothermally regulated stream in Iceland which had a wide range of estimates (GPP: 2 – 28 g O2
m-2 d-1; ER: 3 - 67 g O2 m-2 d-1, Demars et al. 2011) but the upper range for both GPP and ER
were 5 to 10 times higher than our maximum estimates. This suggests that low nutrient systems
may be more productive relative to high nutrient systems.
Water column NEP showed that the stream in the winter was autotrophic likely due to the
open canopy as well as the removal of leaf litter from the benthos allowing more production,
consistent with previous assessments (Roberts et al. 2007). Algal biomass was higher in the
colder months; therefore, winter production may have been influenced more by total biomass,
skewing production estimates. The lower temperature in the winter also decreased microbial
activity which may have contributed to the P: R > 1 which was similar to the decrease in
metabolism observed at colder temperatures in Iceland streams (Demars 2011). In spring,
production was equal to respiration however the ecosystem became slightly autotrophic in the
summer (P: R > 1). This suggested that the autotrophs and heterotrophs were releasing and
assimilating at similar rates (Søndergaard et al. 1995). In the fall, the ecosystem was
heterotrophic likely due to leaf litter inputs which increased the microbial activity (Petersen and
Cummins 1974).
Algal NEP indicated net heterotrophy in the fall, winter, and spring. This was likely due
to the close association between the microbial community and the algae (Søndergaard et al.
1995). In the fall, microbial activity likely increased due to litter fall and decreased primary
production (Petersen and Cummins 1974). In the winter and spring the microbial community
23
could quickly assimilate the exudates released from the algal mat. In the summer, algal NEP was
autotrophic, likely due to primary production as well as algal senescence which released
exudates for metabolism (Fogg 1983). Overall, these variations in NEP between the water
column and algae reflect the spatial scales at which these measurements were collected. The
microbial community was not coupled as tightly with the water column as it was to the algae in
the benthos.
Factors influencing algae and water column physiochemical variation
On a diurnal scale, algal production consistently increased DO and pH during the day and
decreased during the night within the algal mat, consistent with hypotheses and likely
attributable to photosynthesis similar to diurnal patterns in an algal mats previously observed
(Revsbech et al. 1983,Talling 1976). Water column DO concentrations also increased during the
day and decreased during the night with similar amplitude to measurements collected in a
headwater stream before leaf out in March (Roberts et al. 2007) and April (Mulholland et al.
2001). Due to diffusion limits, temperature is negatively correlated with DO, consistent with
observations in the water column but not the algae. Thus, algal DO concentrations may be more
associated with biological processes, for example, metabolism decreases with cold temperatures.
Therefore similar to Demars et al. (2011) where metabolism decreased with temperature, algal
dissolved oxygen concentrations would also decrease with decreasing temperature as it fuels
metabolism. Temperature correlated negatively with algal biomass which would suggest that
although algal abundance increases with decreasing temperature, algae are more efficient in
summer months.
24
Factors influencing algae and water column nutrient concentrations
Water column dissolved nutrient concentrations reflected the seasonal variability of
nitrogen and phosphorus and the influences of agriculture and urbanization and were comparable
to concentrations observed in the same watershed (Veach and Bernot 2011). Changes in algal
pore water nutrient concentrations were consistent with seasonal patterns of production with
assimilation in the spring and release in the summer (Mulholland and Hill 1997, Roberts et al.
2007). Even in this high nutrient system the influence of algae on nutrient concentrations was
detectable. Although NO3 concentrations were elevated due to a storm event, relative to the
water column, algal pore water had lower NO3 concentrations in spring likely due to
assimilation. This decrease in stream nutrient concentrations in the spring has also been observed
in headwater streams (Roberts et al. 2007) but at a different magnitude. We also observed an
increase in algal pore water NO3 concentrations in the summer which was also consistent with
trends observed in Roberts et al. (2007). Similar patterns in assimilation and release of nutrients
in the spring and summer were found by Lee et al. (2012) though the highest concentrations of
NO3 were measured during winter months in the Upper Mississippi River Basin, contrary to our
observations. Algal biomass estimates for Lee et al. (2012) reported no algae in the winter which
may have allowed NO3 to accumulate. In our study, NO3 concentrations were lowest in the
winter likely a function of algal biomass assimilating available nutrients and changes in inputs
from the surrounding watershed.
Anthropogenic influences may have also affected NO3 and TOC concentrations through
runoff associated with storms. The March storm event did not rise above flood stage and
increases in TOC concentrations did not occur; however, NO3 concentrations were significantly
higher. Nitrate is introduced to streams during storm events via tiles and subsurface flow while
25
organic carbon is commonly introduced via overland inputs (Wagner et al. 2008). The algae pore
water had higher NO3 and PO4 concentrations in the summer relative to the water column, likely
due to nutrient release from senescent algae (Bratbak et al. 1994). June sampling took place
following a storm event that rose above flood stage and likely introduced a large amount of NO3
and organic carbon into the stream from agricultural and urban sources. Increased nitrate was
consistent with David et al. (1997) and increased organic carbon concentrations in streams were
consistent with Dalzell et al. (2005 and 2007) where allochthonous inputs were contributed to
from agricultural fields and tiles following flooding events.
Conclusions
This study showed the influence algae have on water column physiochemistry, nutrients
and metabolism. While physiochemical patterns were consistent between the algae and the water
column, the amplitude of the changes varied as well as the contribution of algal-mediated shifts
to the water column. These data also suggested that the microbial community was closely
associated with the algae but not closely associated with the water column based on seasonal
NEP. Algae contributed ~ 13 % to total ecosystem production. Algal contributions were
detectable but not consistent with previous studies (Minshall 1978) as they were conducted in
undisturbed streams.
26
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R.A. Vollenweider. Blackwell Scientific Publications, Oxford. pp. 111–119.
Pennino, M. J., Kaushal, S. S., Beaulieu, J. J., Mayer, P. M., and C. P. Arango. (2014). Effects of
urban stream burial on nitrogen uptake and ecosystem metabolism: implications for
watershed nitrogen and carbon fluxes. Biogeochemistry.
Petersen, Robert C., and K. W. Cummins. (1974). Leaf processing in a woodland stream.
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primacy. Ecology, 73(3), 733-746.
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Roberts, B. J., Mulholland, P. J., and W. R. Hill. (2007). Multiple scales of temporal variability
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forested headwater stream. Ecosystems, 10(4), 588-606.
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Freshwater benthic ecosystem. Academic press.
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30
Upper White River Watershed Alliance (UWRWA). (2011). http://www.uwrwa.org/. Accessed
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31
Fig. 1
32
Fig. 2
33
Fig. 3
34
Table 1.
35
Table 2.
36
Fig. 4
37
Fig. 5
38
Fig. 6
Winter
Spring
Summer
Fall
8
-1
Algae (mg TOC L )
10
6
4
2
0
0
2
4
6
Water column (mg TOC L-1)
39
8
10
Fig. 7
Winter
Spring
Summer
Fall
-1
Algae (mg NO3 L )
80
60
40
20
0
0
20
40
60
Water Column (mg NO3 L-1)
40
80
Fig. 8
30
Winter
Spring
Summer
Fall
-1
Algae (mg PO4 L )
25
20
15
10
5
0
0
5
10
15
20
Water Column (mg PO4 L-1)
41
25
30
Fig. 9
42
Table. 3
43
CHAPTER 2: Influence of algal exudates and benthic substrata on microbial activity
Abstract
Benthic algae are important for ecosystem processes because they regulate nutrient
cycling through assimilation of inorganic nutrients and release of nitrogen and carbon exudates
through photosynthesis. Algal-derived dissolved organic matter (DOM) may facilitate microbial
respiration in streams and thereby influence ecosystem metabolism. The objective of this study
was to isolate mechanisms by which algae may stimulate microbial respiration in a eutrophic
stream ecosystem. We hypothesized that microbial activity would increase in the presence of
algae due to the release of labile exudates into the surrounding water during photosynthesis. We
used experimental mesocosms with a factorial design to study interactions between algal
treatments (actively growing algae, algal exudate, and control) and benthic substrates (leaves,
sediment, cellulose sponge and fritted glass disc) over a 3 d period. We measured
physiochemical variables and nutrient concentrations every 24 h and quantified microbial
respiration as an indicator of microbial activity using dehydrogenase assays (DHA) after 3 days.
Following incubation of mesocosms, dissolved nutrient concentrations varied among both algal
treatments and benthic substrates. Specifically, algal exudate and leaf treatments yielded the
highest total organic carbon concentrations. The algal exudate and leaf treatments also had lower
nitrate and higher phosphate concentrations relative to other treatments and substrates.
Respiration differed among carbon substrates but not algal treatments. We did not detect an
effect of algae or algal exudate abundance on microbial respiration after 3 days, potentially due
to rapid assimilation of exudates prior to our measurements. Leaf substrates yielded the highest
rates of microbial respiration likely due to higher carbon availability associated with leachate.
44
Regardless of algal treatment, when TOC concentrations were > 8 mg /L, microbial respiration
increased suggesting that carbon concentration does influence respiration. These data show the
influence of algae and benthic substrates on microbial respiration in eutrophic environments and
offer insight into the challenges of studying these complex systems. Continued study of
microbial responses to algae and benthic substrates will help predict ecosystem responses with
changing nutrient status as streams become eutrophic.
45
Introduction
Primary production and decomposition are processes that occur in both terrestrial
(Tansley 1935) and aquatic ecosystems (Lindeman 1942, Odum 1956) and can be used as a
measure of ecosystem function (Vannote et al. 1980).Though much research has quantified
primary production and decomposition independently (Singh and Gupta 1977, Cole 1982), recent
studies have highlighted relationships between these processes (Baines and Pace 1991, Findlay et
al. 1993, Bertilsson and Jones 2003, Hagen et al. 2012 ) that enhance our understanding of
ecosystem dynamics and nutrient cycling. Specifically, attention has focused on the potential for
primary prod ction to facilitate decomposition thro gh the “priming effect” (Bingemann et al.
1953). In the context, the priming effect occurs when labile carbon and nitrogen exudates are
released from primary producers into the environment and serve as a catalyst for microbial
activity (Bingemann et al. 1953, Kuzyakov et al. 2000, Guenet et al. 2010, Bianchi 2011)
facilitating increased mineralization of carbon and nitrogen. The mechanisms by which
photosynthetic products increase decomposition of organic matter was first observed in
terrestrial ecosystems where the addition of plant material to soil increased nitrogen
mineralization (Lohnis 1926). Our understanding of the priming effect has continued to develop
in the terrestrial literature (Bingemann et al. 1953, Kuzyakov et al. 2000, Fontaine et al, 2003)
although the phenomenon has not been extensively studied in aquatic ecosystems (but see
Guenet et al. 2010, Bianchi 2011, Danger et al. 2013). This represents a significant gap in our
understanding of primary production and decomposition as well as the important role the
microbial community serves in mediating these processes.
In mid-order streams, algae regulate nutrient cycling through assimilation of inorganic
nutrients (Howarth 1988, Mulholland 1996) and release of carbon and nitrogen exudates
46
(Bertilsson and Jones 2003, Harbott and Grace 2005, Wiegner et al. 2005). While algal nutrient
assimilation is well studied, understanding of algal nutrient and dissolved organic matter (DOM;
also referred to as exudate) release is limited. Nutrient and DOM release by algae may play an
important role in decomposition and may increase assimilation and mineralization of organic
matter by microbial communities. There are many pathways in which algae release DOM into
the environment. Fogg (1983) suggested that healthy algae become supersaturated with labile
organic matter and actively release DOM via the extracellular membrane to continue assimilation
of available nutrients. Alternatively, DOM can be released passively through the extracellular
membrane and not controlled by photosynthesis as light availability has been shown to be
independent of DOM release (Bertilsson and Jones 2003). Senescent algae may also contribute
labile organic matter for use by heterotrophic microorganisms (Bratbak et al. 1994, Wyatt et al.
2014a). Additionally, algal material can be released into the environment via cell lysis during
“sloppy” cons mption y grazers (J mars et al. 1989 Wallace and Webster 1996). All of these
pathways may influence the amount of labile organic matter available to heterotrophic microbes
which influences microbial activity (Bertilsson and Jones 2003) and subsequently influences
decomposition rates (Cole 1982, Jones and Cannon 1986, Baines and Pace 1991).
The nutrient status of the environment can also influence algal DOM release (Fogg 1983,
Obernosterer and Herndl 1995, Wyatt et al. 2014b). In eutrophic environments, the influence of
active algal cell exudate on heterotrophic activity may be higher relative to oligotrophic
environments because biomass can be greater (Obernosterer and Herndl 1995, Wyatt et al. 2010,
Wyatt et al. 2012) due to nutrient saturated primary production (Rosemond et al. 1993, Mosisch
et al. 2001). In oligotrophic environments, primary production is nutrient limited; thus, the
amount of DOM released may be lower as algae retain DOM for continued photosynthesis. In
47
contrast, primary production in eutrophic environments is not nutrient limited; thus, the amount
of DOM released may be due to maintenance of photosynthesis and continued assimilation of
available nutrients (Fogg 1983, Obernosterer and Herndl 1995). In oligotrophic systems, algal
DOM release may be important to ecosystem processes but the amount of DOM released may be
lower relative to eutrophic systems.
Understanding the influence algal DOM may have on heterotrophic respiration will help
predict future ecosystem response to changes in the environment. For example, streams are
becoming increasingly eutrophic which can increase algal production and subsequently microbial
activity which may influence decomposition rates and alter ecosystem processing of nutrients
and organic matter. The objective of this study was to isolate mechanisms by which algal DOM
facilitates microbial activity in the context of available substrates. To do this we used
experimental mesocosms with a factorial design to study interactions between algae and algal
exudates with organic (leaves, sediment or cellulose sponge) and inorganic substrates (glass
fritted disc) by measuring microbial respiration as an indicator of microbial activity. We
hypothesized that microbial activity would increase with algal production or algal exudates due
to increased availability of labile DOM. Since the organic substrates contributed additional
carbon and nutrients, we expected that microbial respiration on organic substrates would be
higher relative to the inorganic substrate and we anticipated that the inorganic substrate would
isolate the relationship between algae and the stream microbial community to provide a
respiration baseline.
Methods
Experimental design
48
Four algal treatments (actively growing algae, senesced algae, algal exudates only and a
control without algae or exudates) were applied to four substrates (sediment, leaves, organic
cellulose sponge and a control inorganic glass fritted disc) in experimental mesocosms. Each
algal treatment and benthic substrate factorial combination (N = 16 combinations) had 5
replicates except the inorganic substrate which had 6 replicates yielding a total of 84 mesocosms.
Each mesocosm was individually aerated using air stones. Mesocosms were 500 mL clear glass
jars filled with 300 mL of stream water collected from the White River in central Indiana.
Filamentous algae and sediment were also collected from the White River for use in the
experiment. Physiochemical measurements (mean
SE) of the stream water including
temperature (22.0 0.06°C), dissolved oxygen (8.01 0.07 mg/L) and pH (8.86 0.01) were
collected over a 3 d sampling event in May 2013 prior to experimental set-up. Initial total
organic carbon (4.05 0.32 mg/L; n = 64), nitrate (15.71
1.06 mg/L; n = 80) and phosphate
(14.41 1.14 mg/L; n = 80) concentrations were also measured via collection of filtered water
samples.
Algal treatments
Filamentous green algae were carefully removed from cobbles to serve as both the
actively growing and senesced algae treatments following incubation in the laboratory. The algal
samples were placed in Whirl-Paks (Nasco, Fort Atkinson, WI) filled with stream water on site
and transported to the laboratory on ice in the dark where they were processed within 1 h. Algae
were divided for actively growing and senesced algal treatments into two 10 gallon (37.8 L)
aquaria for incubation (5 d). Both aquaria were filled with stream water from the White River
and were kept at room temperature (22 °C) under constant (24 h) light conditions. The aquarium
49
for the actively growing algae treatments were incubated with air stones to prevent uptake rates
of nutrients from exceeding mixing and diffusion rates (Stevenson and Glover 1993) and to
promote CO2 exchange. The second aquarium for the senesced algae treatments were incubated
without air stones to facilitate degradation of the algal material. We observed stream algae
degrade rapidly in stagnant conditions in the senesced treatment, changing from dark green to
brown during the incubation and visually different from the algae in the actively growing
aquarium which appeared dark green in color. Following initial algal incubations, algae were
transferred to experimental mesocosms. Each mesocosm received approximately 1 g (wet
weight) of algae. The senesced algae treatments recovered quickly (~1 d) when transferred to
mesocosms (changing from brown back to dark green).
Algal exudates were collected by leaching filamentous green algae and freeze-drying the
dissolved organic matter into a powder form (Shapiro, 1957, Beck et al., 1974). Approximately 4
L of stream ater as collected and filtered thro gh a 0.2 μm Vac Cap filter (Pall Corporation
Port Washington, NY) to remove heterotrophic organisms before any algal material was
collected for DOM leaching. Benthic filamentous green algae were collected from cobbles in the
stream as described above and sediment and macroinvertebrates were removed prior to placing
in Whirl-Paks (Nasco, Fort Atkinson, WI) with stream water for transport to the laboratory on
ice in the dark. In the la oratory eight 1 L eakers ere filled ith 500 mL of 0.2 μm filtered
stream water. The algal tissue collected was distributed evenly among the eight beakers. The
beakers were placed under constant light conditions at room temperature (21 °C) for 12 h.
Following the incubation, the water was strained to remove algal material and then the remaining
water was filtered through a Millipore nitrocellulose member filter (Millipore Corporation,
Billerica MA; nominal pore size 0.45 μm). The filtrate as collected in Erlenmeyer flasks and a
50
sample was taken to quantify the total organic carbon in the sample for yield estimates. The
filtrate was then immediately lyophilized converting the dissolved organic matter into a powder
form using a Labconco Freeze Dryer (Labconco, Kansas City, MO). The resulting filtrate served
as the inoculum for the algal DOM treatment. Each treatment received a concentration of 10
mg/L of DOM. Throughout the 3 d incubation period, as water evaporated from mesocosms,
stream water was refilled to the initial 300 mL volume. Stream water was used to refill all
mesocosms except for the algal exudate treatments which were refilled with stream water
enriched with 10 mg/L of DOM.
Substrate treatments
Three leaf types, American Elm (Ulmus americana), Black Walnut (Juglans nigra), and
Sugar Maple (Acer saccharum) were used for the coarse particulate organic matter (CPOM)
substrate. Healthy leaves were removed from tree limbs in May 2013 and then dried at 70°C
overnight. The dried leaves were then placed in stream water for 3 d to be conditioned by the
microbial community before being distributed among mesocosms. Three leaves, one of each
type, weighing approximately 5 g, were placed into each leaf treatment mesocosm. River
sediment (top ~0 - 5 cm of the benthic surface) from the original collection site was used for the
fine particulate organic matter substrate. The sediment was homogenized using a No. 3 sieve.
Approximately 1 g of sediment (wet weight) was distributed to each sediment treatment
mesocosm. Cellulose sponges (diameter of 2.54 cm; 3M Corporation, St. Paul, Minnesota) were
used for the artificial organic carbon substrate. One sponge (mean weight of 0.38 g) was placed
in each sponge treatment mesocosm. Glass fritted discs (diameter of 2.54 cm; Leco Corporation,
51
St. Joseph, MI) were used for the inorganic carbon substrate. One glass disc, with a mean weight
of 4.9 g, was placed in each glass (inorganic carbon) treatment mesocosm.
Physicochemical measurements
Once every 24 h over the 3 d experiment, measurements of dissolved oxygen (mg O2/L),
temperature (°C) (YSI, DO200) and pH (Extech, pH200) from each mesocosm were recorded.
Composite duplicate water samples from each mesocosm were collected at 24 h and 48 h to
quantify total organic carbon and nutrient concentrations. High-density polyethylene scintillation
vials were filled with water filtered through Whatman GF/F (0.7 μm nominal pore size) efore
freezing for subsequent analyses. Samples for measurement of total organic carbon (TOC)
concentrations were acidified with 1.0 N HCl immediately after filtration. All samples were
frozen within 1 h of filtration. Nitrate (NO3), phosphate (PO4) and ammonium (NH4)
concentrations were measured using a Dionex ion chromatograph (Dionex Corporation,
Sunnyvale, California). Detection limits were 0.01 mg NO3/L, 0.01 mg PO4/L and 0.005 mg
NH4/L. Total organic carbon concentrations were measured using a Shimadzu TOC-L analyzer
(Shimadzu Scientific Instruments, Columbia Maryland) using standard methods (Eaton et al.
2005). The detection limit for the TOC without blanking the instrument between samples was 2.0
mg TOC/L.
Microbial respiration
Microbial respiration of each mesocosm substrate (sediment, leaves, sponge or glass) was
quantified using dehydrogenase activity (DHA) assays (Trevors 1984, Hill et al. 2002, Bunch
and Bernot 2011) at the end of the 3 d incubation. Approximately 2 cm3 of each substrate was
52
removed from each mesocosm and placed into 50 mL falcon tubes with 2.5 mL of stream water
and 1 mL of 0.75% iodonitrotetrazolium (INT) chloride. Samples were inverted until well mixed
and incubated at room temperature for 3 h. Following incubation, 8 mL of methanol was added
to each sample to stop the reaction and each sample was inverted until well mixed. All samples
were centrifuged for 5 min and supernatant was analyzed on a spectrophotometer (UV-1700
PharmaSpec, Shimadzu) at 428 nm. Because methanol extracted chlorophyll from samples,
absorbance values were corrected by averaging the absorbance of ten samples each of 1 g algae,
5 g leaves and 1 g sediment that were incubated with methanol only. Absorbance values were
then converted to μg O2/h using a standard curve of INT formazan.
Statistical analyses
Difference in respiration and nutrient concentrations among algal and substrate
treatments were analyzed using analysis of variance (ANOVA) and subsequent Tukey pairwise
comparisons. Initial statistical tests indicated no differences between senesced and healthy algal
treatments, potentially due to the senesced algae recovery in mesocosms. Thus, all analyses
combined senesced and actively growing algae treatments. All ANOVA and pairwise
comparisons were performed using SAS statistical software.
Results
Physicochemical measurements
Dissolved oxygen concentrations were 1.4 % higher in the algal treatment (range = 7.40 8.00 mg O2/L) relative to the exudate treatment (7.34 - 7.61 mg O2/L) (p = 0.027; Fig. 1). In the
53
control treatment (no algae or exudate), dissolved oxygen concentration ranged from 7.43 to 7.70
mg O2/L and was not different from the algae and exudate treatments (p > 0.05).
Water temperature in DOM treatments was 0.0 – 1.6 °C higher (range = 20.07- 21.67 °C)
than the algae treatment (range = 18.47 - 21.77 °C) (p = 0.01; Table 1). Temperature in the
control treatments ranged from 18.43 - 22.17 °C and was not different from the algae or exudate
treatments (p > 0.05). Temperature did not differ among substrates (p > 0.05).
Among algal treatments, pH was 0.61% higher in the exudate treatment (range = 8.56 8.74, p < 0.001; Fig. 2) relative to algae and control treatments (range = 8.47 - 8.70). Among
substrates, pH was 0.50 % lower in the sponge treatments (range = 8.54 - 8.68) relative to other
treatments (range = 8.47 - 8.74, p < 0.001; Fig. 2).
Total organic carbon and dissolved nutrient concentrations
Algal exudate treatments had 26.4% higher TOC concentrations (mean = 8.45 mg/L, p <
0.0001; Fig. 3) relative to the algae (mean = 6.42 mg/L) and control (mean = 6.54 mg/L)
treatments. Substrate effects were measured with exposure to leaves yielding 33.7% higher TOC
concentrations (mean = 8.87 mg/L, p < 0.0001; Fig. 3) relative to other substrate types (range =
5.91 – 7.04 mg/L).
In contrast to the TOC concentrations, the exudate treatments had 17.5% lower NO3
concentrations (mean = 5.21 mg/L, p = 0.0002; Fig. 4) relative to the algae (mean = 6.25 mg/L)
and control (mean = 6.16 mg/L) treatments (p > 0.05; Fig. 4). Treatments with leaves as the
substrate had 42.2 % lower NO3 concentrations (mean = 5.48 mg/L, p < 0.0001; Fig. 4) relative
to sediment (mean = 8.26 mg/L) and glass (mean = 8.55 mg/L) substrates (p > 0.05). Sponge
substrates had 143.3% lower NO3 concentrations relative to other substrate types.
54
Similar to the TOC concentrations, the exudate treatment had 86.9% higher PO4
concentrations (mean = 0.013 mg/L, p < 0.01; Fig. 5) relative to the algae and control treatments
(mean = 0.005 mg/L, p > 0.05). Leaves substrate yielded 110.1% higher PO4 concentrations
(mean = 0.014 mg/L, p < 0.003; Fig. 5) relative to the sponge (mean = 0.003 mg/L) and glass
(mean = 0.005 mg/L) treatments and 35.0% higher PO4 concentrations relative to the sediment
treatment (mean = 0.010 mg/L) (p > 0.05).
Ratios of TOC to NO3 were not different among treatments (range = 1.56 – 4.27, p =
0.263) but were among substrates (range = 0.73 – 7.29, p < 0.01) with the highest ratio
associated with sponge substrate. Ratios of TOC to PO4 were not different among treatments
(range = 1087.9 – 6894.8, p = 0.126) or substrates (range = 908.0 – 6898.9, p = 0.325).
Relationships among nutrient ratios suggested differences in nutrient limitation among
treatments (Fig. 6). Specifically, algae treatments were primarily phosphorus limited with the
lowest TOC: NO3 and highest TOC: PO4 ratios of all treatments. The algae treatment had the
highest NO3: PO4 ratio (7789.2). In contrast, exudate treatments were primarily nitrogen limited
having the highest TOC: NO3 and lowest TOC: PO4 ratios relative to other treatments. The
exudate treatment had the lowest NO3: PO4 ratio (427.5) which further suggested nitrogen
limitation. The control treatments were likely carbon limited as the ratios of TOC: NO3, TOC:
PO4 and NO3: PO4 were between the algae and exudate treatments.
Respiration
Leaf s
strates had 157.4% higher respiration (mean = 2.72 μg O2/h, p < 0.001; Fig. 6)
relative to other s
strate types (mean = 0.33 μg O2/h). Total organic carbon influenced
microbial respiration rates above a concentration threshold of ~8 mg/L (p < 0.01, Fig. 8; two-
55
dimensional Kolmogorov-Smirnov test, Garvey et al. 1998, Kemp and Dodds 2002). When TOC
concentrations were above 8 mg/L, microbial respiration was higher, though variable as other
factors were likely controlling rates (e.g., nitrogen, phosphorus, substrate). However, when TOC
concentrations were < 8 mg/L respiration was always lower likely due to carbon limitation.
Discussion
Physiochemical dynamics in response to algae and benthic substrates
Algae and substrate treatments influenced dissolved oxygen concentrations and
temperature. Changes in dissolved oxygen may be attributed to biological processes. For
example, heterotrophic metabolism of DOM decreased available oxygen. In contrast, algae
increased dissolved oxygen concentrations which we would expect in conjunction with
photosynthesis. Interestingly, the exudate treatment yielded the lowest dissolved oxygen
concentration suggesting higher heterotrophic metabolism potentially associated with the readily
available DOM.
The exudate treatment also had the highest pH which may have been due to higher
calcium concentrations, relative to the stream water, in the isolated algal exudate buffering pH
shifts associated with production (Talling 1976). In the future, using anion and cation exchange
resins would remove these anions and cations, resulting in a more pure algal exudate. Across the
substrate treatments, pH was comparable except associated with the sponge treatment which had
a significantly lower pH and may be an experimental artifact due to the possible neutralizing
buffer agent present in the sponge. Temperature was also influenced by algae treatments and
may also be an experimental artifact. The mesocosms were randomly distributed and mesocosms
towards the middle of the arrangement may have been insulated by the surrounding mesocosms.
56
Nutrient dynamics in response to algae and substrates
Algal treatments affected TOC, nitrate and phosphate concentrations. Specifically, TOC
was lowest in the algae treatment, potentially due to assimilation during heterotrophic
metabolism. The algae provide a carbon substrate for the microbial community to colonize and
assimilate as quickly as it is released (Søndergaard et al. 1995, Wyatt et al. 2012). Even though
the algae were healthy the cells co ld release organic car on via an “overflo ” mechanism
(Fogg 1983). This overflow mechanism would be more prevalent in eutrophic streams since
nitrogen and phosphorus are not limiting to algal productivity. Excess carbon is released so the
photosynthetic structures are not clogged with excess carbohydrate and continued algal
production can occur.
As expected, TOC concentrations were highest in the exudate treatment. Initially, algal
exudate treatments had a TOC concentration of 5.45 mg/L in addition to the baseline TOC
concentration in the stream water (5.91 mg/L); thus, a cumulative initial concentration of 11.36
mg/L TOC. Over the experiment duration, algal exudate treatment TOC concentrations
decreased to 8.56 mg/L. This change in TOC concentration during the experiment suggest that at
least 2.80 mg/L TOC were assimilated by the microbial community as there was no influence by
a benthic substrate. The algal exudate treatment was added during the experiment to replace the
volume lost through evaporation and altered total organic carbon concentrations and
assimilation. Using the change in organic carbon observed, the microbial community assimilated
at least 24% of the organic carbon available, likely the most labile TOC constituents. The
remaining 76% of TOC may have been recalcitrant and difficult to assimilate quickly. Labile
organic carbon has been observed to be assimilated quickly (days, Wetzel and Manny 1972;
57
hours, Wyatt et al. 2012) with variation in the time scales based on the composition and origin of
the labile organic carbon. For example, Wetzel and Manny (1972) studied leaf leachate while
Wyatt and others (2012) studied algal exudates. The amount of labile organic carbon assimilated
was comparable but slightly higher than the assimilation of labile organic carbon (0.6 – 1.1
mg/L) reported from a eutrophic lake (Søndergaard et al. 1995).
Across substrates, NO3: PO4 was highest in the algal treatments. This may be due to
nitrate release into the water column during algal production or that nitrification was stimulated
in the presence of algae (Kemp and Dodds 2002). Alternatively, algae may have more efficiently
assimilated phosphate resulting in higher ratios. Algal retention of phosphorus has been observed
in stream ecosystems (Rier et al. 2007) as phosphorus can often be limiting to autotrophic
metabolism. Algal exudate treatments increased availability of organic carbon which may have
influenced the ability of the microbial community to assimilate nitrate and phosphate. The
microbial community assimilated nitrate, as evidence by lower concentrations, although
phosphate concentrations increased over the incubation suggesting that phosphate was being
released back to the water column at an equal or greater rate. Phosphate may have been released
back to the water column as microbial communities typically do not retain phosphate as
efficiently as algae (Morris and Lewis 1992, Rier and Stevenson 2002). Further, the algal
exudate ratio of nutrients suggests that the algae treatment mesocosms were primarily NO3
limited and secondarily PO4 limited.
Algae and substrate effects on heterotrophic metabolism
Contrary to our hypothesis, algal production and algal exudate did not affect microbial
respiration. Microbial respiration rates were higher in the algae and exudate treatment relative to
58
the controls, though not statistically different. The lack of an effect of algae on microbial activity
may have been due to limited replication. The labile organic carbon released during algal
production was likely quickly assimilated and the associated increase in microbial respiration
was not observed due to the sampling intervals. Additionally, Rier and Stevenson (2002)
suggested that the link between algae and bacteria are more closely associated in oligotrophic
environments when nutrients are limiting. Perhaps since the mesocosms were eutrophic, the
microbial community was not dependent on algae for carbon and nutrients as they were available
in the water column. Further, the algal exudate treatment limited heterotrophic response may be
attributed to rapid assimilation of the DOM at experiment start followed by microbial respiration
returning to baseline levels.
In contrast to algal treatments, substrate affected microbial respiration with leaves
resulting in higher respiration likely due to higher carbon availability. Leaf leachate increased the
total organic carbon (TOC) concentration in mesocosms relative to other substrates but was
comparable to the algal exudate treatment which did not increase respiration. Leaf leachate can
be assimilated quickly by the microbial community (Strauss and Lamberti 2002) but higher
respiration may also be associated with carbon substrate available for the microbial community
(Farjalla et al. 2008, Yoshiura et al. 2008). Slow mineralization of recalcitrant leaf substrate may
also explain higher microbial respiration relative to other substrates.
Conclusion
This experiment showed the influence algae and benthic substrates have on microbial
respiration in eutrophic environments and offers insight into the challenges of studying these
complex systems. Microbial activity was stimulated with TOC concentrations above 8 mg/L.
59
This response was primarily driven by the leaf substrate. While we did not observe an algal
mediated microbial respiration response, this was likely because we did not capture the event,
not because the response was absent. Organic carbon, especially labile forms, are quickly
assimilated and shorter sampling intervals may help to quantify the influence of algae or algal
exudates on the microbial community. Algae have been shown to provide important resources
for heterotrophic bacteria (Fogg 1983, Obernosterer and Herndl 1995, Rier et al. 2007, Wyatt et
al. 2010, Wyatt et al. 2012) and likely contribute to other processes in streams even in eutrophic
ecosystems. Continued study of microbial responses to algae and benthic substrates will help to
explain the mechanisms by which these communities interact and how these interactions may
influence stream dynamics.
60
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65
Fig. 1
66
Table 1.
67
Fig. 2
68
Fig. 3
69
Fig 4.
70
Fig. 5
71
Fig. 6
72
Fig. 7
73
Fig. 8
Respiration ( g O2/L)
0.0020
Algae
Exudate
Control
0.0015
0.0010
0.0005
0.0000
5
6
7
8
mg TOC/L
74
9
10
Appendix 1.
Water Column Physiochemical Measurements
January
1/19/2013 9:10
1/19/2013 9:20
1/19/2013 9:30
1/19/2013 9:40
1/19/2013 9:50
1/19/2013 10:00
1/19/2013 10:10
1/19/2013 10:20
1/19/2013 10:30
1/19/2013 10:40
1/19/2013 10:50
1/19/2013 11:00
1/19/2013 11:10
1/19/2013 11:20
1/19/2013 11:30
1/19/2013 11:40
1/19/2013 11:50
1/19/2013 12:00
1/19/2013 12:10
1/19/2013 12:20
1/19/2013 12:30
1/19/2013 12:40
1/19/2013 12:50
1/19/2013 13:00
1/19/2013 13:10
1/19/2013 13:20
1/19/2013 13:30
1/19/2013 13:40
1/19/2013 13:50
1/19/2013 14:00
1/19/2013 14:10
Temp
2.71
2.72
2.72
2.72
2.73
2.75
2.76
2.76
2.75
2.76
2.81
2.88
2.94
2.98
3.01
3.05
3.08
3.12
3.16
3.20
3.25
3.30
3.36
3.41
3.46
3.51
3.56
3.61
3.65
3.69
3.74
DO
12.24
12.29
12.30
12.30
12.29
12.31
12.33
12.30
12.33
12.30
12.35
12.38
12.32
12.35
12.33
12.38
12.42
12.39
12.37
12.35
12.43
12.44
12.40
12.41
12.40
12.43
12.50
12.46
12.45
12.47
12.39
pH
9.75
9.76
9.76
9.77
9.78
9.78
9.78
9.77
9.76
9.75
9.74
9.73
9.59
9.57
9.57
9.56
9.56
9.55
9.55
9.55
9.55
9.55
9.54
9.54
9.54
9.53
9.53
9.53
9.52
9.52
9.52
February
2/7/2013 21:00
2/7/2013 21:10
2/7/2013 21:20
2/7/2013 21:30
2/7/2013 21:40
2/7/2013 21:50
2/7/2013 22:00
2/7/2013 22:10
2/7/2013 22:20
2/7/2013 22:30
2/7/2013 22:40
2/7/2013 22:50
2/7/2013 23:00
2/7/2013 23:10
2/7/2013 23:20
2/7/2013 23:30
2/7/2013 23:40
2/7/2013 23:50
2/8/2013 0:00
2/8/2013 0:10
2/8/2013 0:20
2/8/2013 0:30
2/8/2013 0:40
2/8/2013 0:50
2/8/2013 1:00
2/8/2013 1:10
2/8/2013 1:20
2/8/2013 1:30
2/8/2013 1:40
2/8/2013 1:50
2/8/2013 2:00
Temp
4.99
5.01
5.03
5.04
5.05
5.06
5.06
5.07
5.08
5.09
5.10
5.12
5.13
5.14
5.16
5.17
5.18
5.20
5.21
5.23
5.24
5.26
5.28
5.29
5.31
5.32
5.34
5.34
5.35
5.36
5.37
75
DO
12.04
12.03
11.98
11.86
11.90
11.88
11.89
11.88
11.79
11.83
11.81
11.76
11.83
11.76
11.78
11.76
11.72
11.71
11.66
11.65
11.70
11.65
11.62
11.62
11.61
11.59
11.58
11.53
11.54
11.52
11.53
pH
9.44
9.51
9.54
9.57
9.57
9.56
9.58
9.57
9.58
9.59
9.59
9.59
9.59
9.59
9.59
9.59
9.59
9.59
9.59
9.58
9.58
9.58
9.58
9.58
9.58
9.58
9.58
9.58
9.58
9.57
9.58
March
2/28/13 21:00
2/28/13 21:10
2/28/13 21:20
2/28/13 21:30
2/28/13 21:40
2/28/13 21:50
2/28/13 22:00
2/28/13 22:10
2/28/13 22:20
2/28/13 22:30
2/28/13 22:40
2/28/13 22:50
2/28/13 23:00
2/28/13 23:10
2/28/13 23:20
2/28/13 23:30
2/28/13 23:40
2/28/13 23:50
3/1/13 0:00
3/1/13 0:10
3/1/13 0:20
3/1/13 0:30
3/1/13 0:40
3/1/13 0:50
3/1/13 1:00
3/1/13 1:10
3/1/13 1:20
3/1/13 1:30
3/1/13 1:40
3/1/13 1:50
3/1/13 2:00
Temp
3.31
3.31
3.31
3.31
3.30
3.30
3.30
3.29
3.29
3.28
3.27
3.27
3.26
3.26
3.25
3.25
3.24
3.24
3.23
3.23
3.23
3.22
3.22
3.22
3.21
3.23
3.29
3.34
3.36
3.33
3.28
DO
pH
12.96
12.97
12.97
12.91
12.94
12.98
12.91
12.97
12.89
12.94
12.93
12.98
12.94
12.91
12.97
12.91
12.95
12.93
12.95
12.94
12.93
12.91
12.98
12.95
12.92
12.94
12.83
12.73
12.70
12.74
12.79
1/19/2013 14:20
1/19/2013 14:30
1/19/2013 14:40
1/19/2013 14:50
1/19/2013 15:00
1/19/2013 15:10
1/19/2013 15:20
1/19/2013 15:30
1/19/2013 15:40
1/19/2013 15:50
1/19/2013 16:00
1/19/2013 16:10
1/19/2013 16:20
1/19/2013 16:30
1/19/2013 16:40
1/19/2013 16:50
1/19/2013 17:00
1/19/2013 17:10
1/19/2013 17:20
1/19/2013 17:30
1/19/2013 17:40
1/19/2013 17:50
1/19/2013 18:00
1/19/2013 18:10
1/19/2013 18:20
1/19/2013 18:30
1/19/2013 18:40
1/19/2013 18:50
1/19/2013 19:00
1/19/2013 19:10
1/19/2013 19:20
1/19/2013 19:30
1/19/2013 19:40
1/19/2013 19:50
3.78
3.82
3.85
3.90
3.94
3.96
3.98
4.01
4.02
4.04
4.06
4.07
4.09
4.09
4.10
4.11
4.13
4.14
4.15
4.16
4.18
4.19
4.20
4.22
4.23
4.25
4.26
4.27
4.28
4.30
4.30
4.31
4.33
4.34
12.40
12.46
12.40
12.42
12.46
12.40
12.41
12.34
12.38
12.36
12.34
12.35
12.30
12.35
12.31
12.29
12.27
12.25
12.21
12.17
12.24
12.12
12.13
12.13
12.09
12.05
12.04
12.01
11.98
11.96
11.94
11.98
11.95
11.90
9.52
9.52
9.51
9.51
9.50
9.50
9.50
9.49
9.49
9.50
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.48
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
9.49
2/8/2013 2:10
2/8/2013 2:20
2/8/2013 2:30
2/8/2013 2:40
2/8/2013 2:50
2/8/2013 3:00
2/8/2013 3:10
2/8/2013 3:20
2/8/2013 3:30
2/8/2013 3:40
2/8/2013 3:50
2/8/2013 4:00
2/8/2013 4:10
2/8/2013 4:20
2/8/2013 4:30
2/8/2013 4:40
2/8/2013 4:50
2/8/2013 5:00
2/8/2013 5:10
2/8/2013 5:20
2/8/2013 5:30
2/8/2013 5:40
2/8/2013 5:50
2/8/2013 6:00
2/8/2013 6:10
2/8/2013 6:20
2/8/2013 6:30
2/8/2013 6:40
2/8/2013 6:50
2/8/2013 7:00
2/8/2013 7:10
2/8/2013 7:20
2/8/2013 7:30
2/8/2013 7:40
5.37
5.37
5.38
5.38
5.38
5.39
5.39
5.40
5.40
5.40
5.39
5.38
5.37
5.36
5.34
5.32
5.32
5.32
5.34
5.36
5.39
5.40
5.42
5.44
5.44
5.44
5.43
5.42
5.41
5.39
5.38
5.36
5.34
5.33
76
11.50
11.52
11.50
11.49
11.46
11.49
11.48
11.46
11.43
11.44
11.43
11.43
11.42
11.43
11.46
11.47
11.43
11.40
11.43
11.40
11.34
11.28
11.28
11.23
11.17
11.15
11.14
11.14
11.10
11.06
11.11
11.10
11.10
11.15
9.58
9.57
9.58
9.59
9.59
9.59
9.59
9.59
9.59
9.58
9.59
9.59
9.58
9.60
9.59
9.59
9.59
9.58
9.56
9.56
9.56
9.54
9.53
9.53
9.52
9.51
9.50
9.49
9.49
9.48
9.48
9.48
9.48
9.49
3/1/13 2:10
3/1/13 2:20
3/1/13 2:30
3/1/13 2:40
3/1/13 2:50
3/1/13 3:00
3/1/13 3:10
3/1/13 3:20
3/1/13 3:30
3/1/13 3:40
3/1/13 3:50
3/1/13 4:00
3/1/13 4:10
3/1/13 4:20
3/1/13 4:30
3/1/13 4:40
3/1/13 4:50
3/1/13 5:00
3/1/13 5:10
3/1/13 5:20
3/1/13 5:30
3/1/13 5:40
3/1/13 5:50
3/1/13 6:00
3/1/13 6:10
3/1/13 6:20
3/1/13 6:30
3/1/13 6:40
3/1/13 6:50
3/1/13 7:00
3/1/13 7:10
3/1/13 7:20
3/1/13 7:30
3/1/13 7:40
3.25
3.25
3.27
3.28
3.28
3.28
3.26
3.24
3.23
3.21
3.20
3.19
3.18
3.17
3.16
3.15
3.15
3.15
3.15
3.15
3.15
3.14
3.14
3.14
3.15
3.15
3.15
3.16
3.15
3.15
3.15
3.16
3.15
3.16
12.79
12.87
12.83
12.82
12.85
12.86
12.86
12.89
12.84
12.90
12.94
12.95
12.94
12.88
12.90
12.90
12.92
12.95
12.98
12.95
12.92
12.89
12.96
12.95
12.89
12.90
12.96
12.93
12.90
12.95
12.88
12.91
12.91
12.91
1/19/2013 20:00
1/19/2013 20:10
1/19/2013 20:20
1/19/2013 20:30
1/19/2013 20:40
1/19/2013 20:50
1/19/2013 21:00
1/19/2013 21:10
1/19/2013 21:20
1/19/2013 21:30
1/19/2013 21:40
1/19/2013 21:50
1/19/2013 22:00
1/19/2013 22:10
1/19/2013 22:20
1/19/2013 22:30
1/19/2013 22:40
1/19/2013 22:50
1/19/2013 23:00
1/19/2013 23:10
1/19/2013 23:20
1/19/2013 23:30
1/19/2013 23:40
1/19/2013 23:50
1/20/2013 0:00
1/20/2013 0:10
1/20/2013 0:20
1/20/2013 0:30
1/20/2013 0:40
1/20/2013 0:50
1/20/2013 1:00
1/20/2013 1:10
1/20/2013 1:20
1/20/2013 1:30
4.34
4.31
4.29
4.30
4.35
4.39
4.43
4.46
4.47
4.48
4.48
4.47
4.47
4.46
4.45
4.45
4.44
4.44
4.44
4.43
4.42
4.42
4.41
4.40
4.38
4.38
4.37
4.36
4.35
4.34
4.32
4.31
4.31
4.30
11.93
11.92
11.90
11.92
11.88
11.86
11.82
11.79
11.78
11.77
11.80
11.78
11.78
11.74
11.77
11.75
11.74
11.74
11.70
11.70
11.74
11.67
11.67
11.70
11.75
11.69
11.68
11.66
11.69
11.69
11.67
11.67
11.65
11.66
9.49
9.50
9.50
9.50
9.49
9.49
9.49
9.48
9.48
9.48
9.48
9.49
9.49
9.49
9.49
9.49
9.49
9.50
9.50
9.50
9.50
9.51
9.51
9.51
9.51
9.51
9.51
9.51
9.51
9.52
9.52
9.52
9.53
9.53
2/8/2013 7:50
2/8/2013 8:00
2/8/2013 8:10
2/8/2013 8:20
2/8/2013 8:30
2/8/2013 8:40
2/8/2013 8:50
2/8/2013 9:00
2/8/2013 9:10
2/8/2013 9:20
2/8/2013 9:30
2/8/2013 9:40
2/8/2013 9:50
2/8/2013 10:00
2/8/2013 10:10
2/8/2013 10:20
2/8/2013 10:30
2/8/2013 10:40
2/8/2013 10:50
2/8/2013 11:00
2/8/2013 11:10
2/8/2013 11:20
2/8/2013 11:30
2/8/2013 11:40
2/8/2013 11:50
2/8/2013 12:00
2/8/2013 12:10
2/8/2013 12:20
2/8/2013 12:30
2/8/2013 12:40
2/8/2013 12:50
2/8/2013 13:00
2/8/2013 13:10
2/8/2013 13:20
5.31
5.29
5.26
5.25
5.22
5.21
5.19
5.18
5.18
5.17
5.15
5.14
5.12
5.11
5.10
5.09
5.08
5.08
5.07
5.07
5.06
5.04
5.01
5.01
5.01
5.01
5.01
5.01
5.01
4.99
4.98
4.96
4.95
4.95
77
11.14
11.11
11.10
11.19
11.18
11.27
11.23
11.26
11.27
11.31
11.30
11.32
11.31
11.36
11.38
11.41
11.45
11.51
11.49
11.52
11.61
11.61
11.71
11.74
11.80
11.80
11.88
11.84
11.89
11.90
12.01
12.00
12.04
12.12
9.49
9.50
9.51
9.51
9.51
9.52
9.52
9.53
9.53
9.53
9.53
9.54
9.55
9.55
9.55
9.56
9.56
9.57
9.58
9.59
9.59
9.61
9.63
9.63
9.63
9.63
9.64
9.64
9.65
9.65
9.66
9.66
9.67
9.67
3/1/13 7:50
3/1/13 8:00
3/1/13 8:10
3/1/13 8:20
3/1/13 8:30
3/1/13 8:40
3/1/13 8:50
3/1/13 9:00
3/1/13 9:10
3/1/13 9:20
3/1/13 9:30
3/1/13 9:40
3/1/13 9:50
3/1/13 10:00
3/1/13 10:10
3/1/13 10:20
3/1/13 10:30
3/1/13 10:40
3/1/13 10:50
3/1/13 11:00
3/1/13 11:10
3/1/13 11:20
3/1/13 11:30
3/1/13 11:40
3/1/13 11:50
3/1/13 12:00
3/1/13 12:10
3/1/13 12:20
3/1/13 12:30
3/1/13 12:40
3/1/13 12:50
3/1/13 13:00
3/1/13 13:10
3/1/13 13:20
3.15
3.16
3.16
3.16
3.17
3.17
3.17
3.17
3.17
3.17
3.17
3.17
3.18
3.18
3.18
3.16
3.17
3.20
3.23
3.27
3.29
3.31
3.33
3.33
3.32
3.33
3.33
3.34
3.35
3.35
3.36
3.37
3.39
3.41
12.90
12.94
12.93
12.92
12.97
12.91
12.92
12.92
12.94
12.96
12.93
12.94
12.96
13.00
12.99
13.06
13.06
13.03
13.02
13.05
13.01
13.04
13.01
12.96
13.05
13.08
13.07
13.05
13.07
13.11
13.04
13.04
13.08
13.17
1/20/2013 1:40
1/20/2013 1:50
1/20/2013 2:00
1/20/2013 2:10
1/20/2013 2:20
1/20/2013 2:30
1/20/2013 2:40
1/20/2013 2:50
1/20/2013 3:00
1/20/2013 3:10
1/20/2013 3:20
1/20/2013 3:30
1/20/2013 3:40
1/20/2013 3:50
1/20/2013 4:00
1/20/2013 4:10
1/20/2013 4:20
1/20/2013 4:30
1/20/2013 4:40
1/20/2013 4:50
1/20/2013 5:00
1/20/2013 5:10
1/20/2013 5:20
1/20/2013 5:30
1/20/2013 5:40
1/20/2013 5:50
1/20/2013 6:00
1/20/2013 6:10
1/20/2013 6:20
1/20/2013 6:30
1/20/2013 6:40
1/20/2013 6:50
1/20/2013 7:00
1/20/2013 7:10
4.30
4.29
4.29
4.26
4.25
4.22
4.20
4.18
4.15
4.13
4.10
4.07
4.03
3.98
3.91
3.88
3.86
3.87
3.88
3.86
3.83
3.79
3.75
3.70
3.66
3.61
3.55
3.51
3.47
3.44
3.40
3.35
3.31
3.27
11.66
11.66
11.69
11.65
11.70
11.64
11.71
11.69
11.65
11.68
11.67
11.69
11.67
11.70
11.72
11.69
11.68
11.67
11.72
11.71
11.70
11.66
11.72
11.75
11.77
11.78
11.75
11.80
11.77
11.82
11.79
11.85
11.79
11.85
9.53
9.53
9.53
9.54
9.54
9.54
9.54
9.54
9.55
9.55
9.55
9.55
9.56
9.57
9.57
9.57
9.57
9.56
9.56
9.56
9.56
9.56
9.56
9.57
9.57
9.57
9.58
9.59
9.59
9.59
9.59
9.60
9.60
9.60
2/8/2013 13:30
2/8/2013 13:40
2/8/2013 13:50
2/8/2013 14:00
2/8/2013 14:10
2/8/2013 14:20
2/8/2013 14:30
2/8/2013 14:40
2/8/2013 14:50
2/8/2013 15:00
2/8/2013 15:10
2/8/2013 15:20
2/8/2013 15:30
2/8/2013 15:40
2/8/2013 15:50
2/8/2013 16:00
2/8/2013 16:10
2/8/2013 16:20
2/8/2013 16:30
2/8/2013 16:40
2/8/2013 16:50
2/8/2013 17:00
2/8/2013 17:10
2/8/2013 17:20
2/8/2013 17:30
2/8/2013 17:40
2/8/2013 17:50
2/8/2013 18:00
2/8/2013 18:10
2/8/2013 18:20
2/8/2013 18:30
2/8/2013 18:40
2/8/2013 18:50
2/8/2013 19:00
4.97
4.98
4.98
4.99
5.01
5.02
5.03
5.04
5.04
5.03
5.03
5.04
5.04
5.04
5.03
5.02
5.00
4.98
4.96
4.94
4.91
4.89
4.87
4.85
4.82
4.80
4.77
4.73
4.70
4.66
4.62
4.58
4.54
4.50
78
12.09
12.13
12.18
12.18
12.20
12.24
12.25
12.25
12.23
12.31
12.34
12.41
12.33
12.37
12.40
12.37
12.37
12.34
12.37
12.33
12.33
12.31
12.25
12.32
12.28
12.28
12.24
12.27
12.22
12.19
12.15
12.14
12.16
12.13
9.68
9.68
9.67
9.69
9.69
9.70
9.69
9.70
9.69
9.70
9.70
9.70
9.71
9.71
9.71
9.72
9.73
9.73
9.73
9.73
9.73
9.72
9.73
9.74
9.73
9.74
9.74
9.74
9.74
9.74
9.74
9.74
9.74
9.74
3/1/13 13:30
3/1/13 13:40
3/1/13 13:50
3/1/13 14:00
3/1/13 14:10
3/1/13 14:20
3/1/13 14:30
3/1/13 14:40
3/1/13 14:50
3/1/13 15:00
3/1/13 15:10
3/1/13 15:20
3/1/13 15:30
3/1/13 15:40
3/1/13 15:50
3/1/13 16:00
3/1/13 16:10
3/1/13 16:20
3/1/13 16:30
3/1/13 16:40
3/1/13 16:50
3/1/13 17:00
3/1/13 17:10
3/1/13 17:20
3/1/13 17:30
3/1/13 17:40
3/1/13 17:50
3/1/13 18:00
3/1/13 18:10
3/1/13 18:20
3/1/13 18:30
3/1/13 18:40
3/1/13 18:50
3/1/13 19:00
3.44
3.47
3.48
3.50
3.52
3.54
3.55
3.57
3.58
3.58
3.60
3.59
3.60
3.60
3.60
3.60
3.60
3.60
3.61
3.60
3.61
3.61
3.61
3.61
3.61
3.61
3.60
3.60
3.60
3.59
3.56
3.54
3.55
3.57
13.12
13.15
13.15
13.15
13.17
13.16
13.15
13.14
13.19
13.17
13.21
13.10
13.19
13.17
13.16
13.15
13.18
13.18
13.13
13.17
13.19
13.13
13.13
13.13
13.14
13.12
13.10
13.08
13.12
13.07
13.05
13.03
13.08
13.00
1/20/2013 7:20
1/20/2013 7:30
1/20/2013 7:40
1/20/2013 7:50
1/20/2013 8:00
1/20/2013 8:10
1/24/2013 21:00
1/24/2013 21:10
1/24/2013 21:20
1/24/2013 21:30
1/24/2013 21:40
1/24/2013 21:50
1/24/2013 22:00
1/24/2013 22:10
1/24/2013 22:20
1/24/2013 22:30
1/24/2013 22:40
1/24/2013 22:50
1/24/2013 23:00
1/24/2013 23:10
1/24/2013 23:20
1/24/2013 23:30
1/24/2013 23:40
1/24/2013 23:50
1/25/2013 0:00
1/25/2013 0:10
1/25/2013 0:20
1/25/2013 0:30
1/25/2013 0:40
1/25/2013 0:50
1/25/2013 1:00
1/25/2013 1:10
1/25/2013 1:20
1/25/2013 1:30
3.24
3.19
3.16
3.11
3.07
3.03
0.69
0.67
0.66
0.64
0.65
0.63
0.61
0.61
0.58
0.55
0.54
0.55
0.58
0.61
0.63
0.65
0.68
0.69
0.69
0.69
0.68
0.68
0.67
0.67
0.66
0.66
0.67
0.66
11.83
11.89
11.85
11.88
11.90
11.92
12.98
13.04
12.96
13.00
12.96
12.92
12.97
12.88
13.01
12.99
12.94
12.93
12.94
12.94
12.87
12.90
12.84
12.85
12.90
12.81
12.89
12.82
12.84
12.86
12.85
12.82
12.83
12.82
9.60
9.61
9.61
9.62
9.62
9.62
9.62
9.65
9.66
9.66
9.64
9.64
9.64
9.64
9.64
9.64
9.64
9.64
9.65
9.65
9.65
9.66
9.66
9.66
9.65
9.66
9.66
9.66
9.60
9.66
9.67
9.67
9.68
9.68
2/8/2013 19:10
2/8/2013 19:20
2/8/2013 19:30
2/8/2013 19:40
2/8/2013 19:50
2/8/2013 20:00
2/8/2013 20:10
2/8/2013 20:20
2/8/2013 20:30
2/8/2013 20:40
2/8/2013 20:50
2/8/2013 21:00
2/8/2013 21:10
2/8/2013 21:20
2/8/2013 21:30
2/8/2013 21:40
2/8/2013 21:50
2/8/2013 22:00
2/8/2013 22:10
2/8/2013 22:20
2/8/2013 22:30
2/8/2013 22:40
2/8/2013 22:50
2/8/2013 23:00
2/8/2013 23:10
2/8/2013 23:20
2/8/2013 23:30
2/8/2013 23:40
2/8/2013 23:50
2/9/2013 0:00
2/9/2013 0:10
2/9/2013 0:20
2/9/2013 0:30
2/9/2013 0:40
4.47
4.43
4.41
4.38
4.37
4.32
4.26
4.23
4.22
4.23
4.23
4.23
4.21
4.18
4.16
4.12
4.09
4.06
4.04
4.01
4.00
3.97
3.95
3.93
3.90
3.88
3.85
3.83
3.81
3.78
3.76
3.73
3.71
3.68
79
12.14
12.11
12.07
12.09
12.05
12.06
12.10
12.07
11.99
12.00
12.03
12.00
12.03
12.02
12.00
12.01
11.98
12.00
11.97
12.02
12.03
12.05
12.06
12.02
12.04
12.06
12.04
12.05
12.03
12.08
12.03
12.08
12.05
12.10
9.74
9.74
9.74
9.74
9.74
9.75
9.74
9.75
9.74
9.73
9.74
9.74
9.74
9.74
9.75
9.75
9.76
9.76
9.75
9.76
9.75
9.76
9.75
9.76
9.76
9.77
9.77
9.77
9.77
9.78
9.77
9.78
9.78
9.78
3/1/13 19:10
3/1/13 19:20
3/1/13 19:30
3/1/13 19:40
3/1/13 19:50
3/1/13 20:00
3/1/13 20:10
3/1/13 20:20
3/1/13 20:30
3/1/13 20:40
3/1/13 20:50
3/1/13 21:00
3/1/13 21:10
3/1/13 21:20
3/1/13 21:30
3/1/13 21:40
3/1/13 21:50
3/1/13 22:00
3/1/13 22:10
3/1/13 22:20
3/1/13 22:30
3/1/13 22:40
3/1/13 22:50
3/1/13 23:00
3/1/13 23:10
3/1/13 23:20
3/1/13 23:30
3/1/13 23:40
3/1/13 23:50
3/2/13 0:00
3/2/13 0:10
3/2/13 0:20
3/2/13 0:30
3/2/13 0:40
3.59
3.61
3.62
3.62
3.62
3.60
3.58
3.56
3.55
3.54
3.52
3.51
3.49
3.48
3.47
3.46
3.46
3.45
3.44
3.43
3.42
3.41
3.40
3.38
3.37
3.37
3.36
3.35
3.34
3.32
3.32
3.31
3.31
3.30
13.02
12.97
13.00
12.98
12.97
12.94
12.95
12.92
12.94
12.97
12.92
12.92
12.95
12.91
12.93
12.94
12.94
12.94
12.89
12.94
12.96
12.97
12.94
12.91
12.89
12.94
12.88
12.92
12.92
12.88
12.89
12.90
12.88
12.91
1/25/2013 1:40
1/25/2013 1:50
1/25/2013 2:00
1/25/2013 2:10
1/25/2013 2:20
1/25/2013 2:30
1/25/2013 2:40
1/25/2013 2:50
1/25/2013 3:00
1/25/2013 3:10
1/25/2013 3:20
1/25/2013 3:30
1/25/2013 3:40
1/25/2013 3:50
1/25/2013 4:00
1/25/2013 4:10
1/25/2013 4:20
1/25/2013 4:30
1/25/2013 4:40
1/25/2013 4:50
1/25/2013 5:00
1/25/2013 5:10
1/25/2013 5:20
1/25/2013 5:30
1/25/2013 5:40
1/25/2013 5:50
1/25/2013 6:00
1/25/2013 6:10
1/25/2013 6:20
1/25/2013 6:30
1/25/2013 6:40
1/25/2013 6:50
0.66
0.68
0.68
0.68
0.69
0.69
0.69
0.70
0.71
0.70
0.71
0.71
0.71
0.71
0.72
0.72
0.71
0.69
0.65
0.65
0.66
0.67
0.69
0.72
0.73
0.76
0.76
0.77
0.74
0.70
0.68
0.70
12.84
12.81
12.80
12.85
12.79
12.81
12.84
12.77
12.79
12.78
12.80
12.80
12.78
12.75
12.81
12.82
12.77
12.82
12.82
12.77
12.76
12.77
12.76
12.76
12.73
12.73
12.69
12.71
12.73
12.70
12.74
12.68
9.68
9.69
9.71
9.70
9.69
9.69
9.69
9.69
9.69
9.68
9.68
9.71
9.70
9.69
9.69
9.69
9.72
9.69
9.69
9.71
9.72
9.70
9.72
9.73
9.71
9.70
9.70
9.71
9.73
9.71
9.71
9.73
2/9/2013 0:50
2/9/2013 1:00
2/9/2013 1:10
2/9/2013 1:20
2/9/2013 1:30
2/9/2013 1:40
2/9/2013 1:50
2/9/2013 2:00
2/9/2013 2:10
2/9/2013 2:20
2/9/2013 2:30
2/9/2013 2:40
2/9/2013 2:50
2/9/2013 3:00
2/9/2013 3:10
2/9/2013 3:20
2/9/2013 3:30
2/9/2013 3:40
2/9/2013 3:50
2/9/2013 4:00
2/9/2013 4:10
2/9/2013 4:20
2/9/2013 4:30
2/9/2013 4:40
2/9/2013 4:50
2/9/2013 5:00
2/9/2013 5:10
2/9/2013 5:20
2/9/2013 5:30
2/9/2013 5:40
2/9/2013 5:50
2/9/2013 6:00
3.66
3.64
3.62
3.60
3.57
3.55
3.52
3.50
3.47
3.45
3.42
3.39
3.34
3.29
3.28
3.29
3.30
3.31
3.30
3.28
3.25
3.23
3.20
3.17
3.15
3.12
3.09
3.07
3.05
3.02
3.00
2.98
80
12.08
12.05
12.07
12.06
12.07
12.08
12.10
12.10
12.07
12.09
12.08
12.13
12.15
12.12
12.11
12.16
12.13
12.08
12.19
12.12
12.11
12.13
12.16
12.14
12.16
12.19
12.14
12.16
12.21
12.23
12.20
12.18
9.78
9.78
9.78
9.78
9.78
9.78
9.78
9.78
9.78
9.78
9.78
9.78
9.79
9.78
9.77
9.77
9.76
9.76
9.75
9.76
9.77
9.77
9.78
9.78
9.79
9.78
9.79
9.79
9.79
9.79
9.80
9.80
3/2/13 0:50
3/2/13 1:00
3/2/13 1:10
3/2/13 1:20
3/2/13 1:30
3/2/13 1:40
3/2/13 1:50
3/2/13 2:00
3/2/13 2:10
3/2/13 2:20
3/2/13 2:30
3/2/13 2:40
3/2/13 2:50
3/2/13 3:00
3/2/13 3:10
3/2/13 3:20
3/2/13 3:30
3/2/13 3:40
3/2/13 3:50
3/2/13 4:00
3/2/13 4:10
3/2/13 4:20
3/2/13 4:30
3/2/13 4:40
3/2/13 4:50
3/2/13 5:00
3/2/13 5:10
3/2/13 5:20
3/2/13 5:30
3/2/13 5:40
3/2/13 5:50
3/2/13 6:00
3.29
3.29
3.27
3.26
3.25
3.23
3.22
3.20
3.17
3.12
3.12
3.14
3.15
3.16
3.16
3.15
3.14
3.12
3.10
3.08
3.06
3.05
3.03
3.02
3.00
2.99
2.97
2.96
2.96
2.95
2.94
2.93
12.93
12.90
12.90
12.90
12.92
12.87
12.92
12.91
12.89
12.95
12.96
12.99
12.91
12.92
12.91
12.93
12.94
12.90
12.93
12.94
12.98
12.94
12.93
12.96
12.95
12.97
12.97
12.98
12.96
12.98
12.97
12.95
Appendix 1. (continued)
April
4/15/2013 0:00
4/15/2013 0:10
4/15/2013 0:20
4/15/2013 0:30
4/15/2013 0:40
4/15/2013 0:50
4/15/2013 1:00
4/15/2013 1:10
4/15/2013 1:20
4/15/2013 1:30
4/15/2013 1:40
4/15/2013 1:50
4/15/2013 2:00
4/15/2013 2:10
4/15/2013 2:20
4/15/2013 2:30
4/15/2013 2:40
4/15/2013 2:50
4/15/2013 3:00
4/15/2013 3:10
4/15/2013 3:20
4/15/2013 3:30
4/15/2013 3:40
4/15/2013 3:50
4/15/2013 4:00
4/15/2013 4:10
4/15/2013 4:20
4/15/2013 4:30
Temp
10.85
10.84
10.82
10.81
10.79
10.78
10.77
10.75
10.74
10.73
10.72
10.7
10.69
10.68
10.66
10.65
10.64
10.63
10.62
10.61
10.59
10.59
10.58
10.57
10.56
10.55
10.54
10.53
DO
9.62
9.64
9.58
9.58
9.58
9.57
9.58
9.56
9.58
9.55
9.53
9.49
9.52
9.51
9.54
9.53
9.52
9.49
9.46
9.45
9.51
9.47
9.49
9.43
9.46
9.46
9.43
9.39
pH
9.01
9.03
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
May
Temp
5/29/2013 21:00 22.29
5/29/2013 21:10 22.25
5/29/2013 21:20
22.2
5/29/2013 21:30 22.15
5/29/2013 21:40
22.1
5/29/2013 21:50 22.05
5/29/2013 22:00
22
5/29/2013 22:10 21.95
5/29/2013 22:20 21.91
5/29/2013 22:30 21.87
5/29/2013 22:40 21.84
5/29/2013 22:50
21.8
5/29/2013 23:00 21.76
5/29/2013 23:10 21.73
5/29/2013 23:20
21.7
5/29/2013 23:30 21.66
5/29/2013 23:40 21.64
5/29/2013 23:50 21.61
5/30/2013 0:00 21.58
5/30/2013 0:10 21.55
5/30/2013 0:20 21.52
5/30/2013 0:30 21.49
5/30/2013 0:40 21.47
5/30/2013 0:50 21.44
5/30/2013 1:00 21.41
5/30/2013 1:10 21.39
5/30/2013 1:20 21.36
5/30/2013 1:30 21.34
81
DO
8.34
8.25
8.18
8.11
8.05
7.97
7.88
7.9
7.79
7.76
7.67
7.61
7.54
7.49
7.46
7.39
7.36
7.34
7.29
7.32
7.26
7.19
7.2
7.18
7.17
7.14
7.07
7.06
pH
8.78
8.8
8.8
8.8
8.8
8.79
8.78
8.78
8.78
8.77
8.77
8.77
8.76
8.77
8.76
8.76
8.75
8.76
8.75
8.75
8.75
8.75
8.74
8.75
8.74
8.74
8.74
8.74
June
6/17/2013 16:10
6/17/2013 16:20
6/17/2013 16:30
6/17/2013 16:40
6/17/2013 16:50
6/17/2013 17:00
6/17/2013 17:10
6/17/2013 17:20
6/17/2013 17:30
6/17/2013 17:40
6/17/2013 17:50
6/17/2013 18:00
6/17/2013 18:10
6/17/2013 18:20
6/17/2013 18:30
6/17/2013 18:40
6/17/2013 18:50
6/17/2013 19:00
6/17/2013 19:10
6/17/2013 19:20
6/17/2013 19:30
6/17/2013 19:40
6/17/2013 19:50
6/17/2013 20:00
6/17/2013 20:10
6/17/2013 20:20
6/17/2013 20:30
6/17/2013 20:40
Temp
23.56
23.59
23.6
23.61
23.59
23.56
23.55
23.55
23.54
23.54
23.53
23.53
23.52
23.52
23.53
23.53
23.54
23.57
23.6
23.62
23.62
23.6
23.58
23.56
23.54
23.53
23.51
23.51
DO
8.07
8.09
8.06
8.02
7.95
7.91
7.86
7.86
7.82
7.82
7.77
7.68
7.64
7.59
7.57
7.52
7.49
7.41
7.37
7.33
7.24
7.24
7.19
7.17
7.14
7.06
7.07
7.02
pH
9.1
9.06
9.07
9.07
9.07
9.07
9.06
9.07
9.06
9.06
9.06
9.06
9.06
9.05
9.05
9.05
9.05
9.05
9.05
9.05
9.04
9.04
9.03
9.03
9.02
9.02
9.02
9.01
4/15/2013 4:40
4/15/2013 4:50
4/15/2013 5:00
4/15/2013 5:10
4/15/2013 5:20
4/15/2013 5:30
4/15/2013 5:40
4/15/2013 5:50
4/15/2013 6:00
4/15/2013 6:10
4/15/2013 6:20
4/15/2013 6:30
4/15/2013 6:40
4/15/2013 6:50
4/15/2013 7:00
4/15/2013 7:10
4/15/2013 7:20
4/15/2013 7:30
4/15/2013 7:40
4/15/2013 7:50
4/15/2013 8:00
4/15/2013 8:10
4/15/2013 8:20
4/15/2013 8:30
4/15/2013 8:40
4/28/2013 21:00
4/28/2013 21:10
4/28/2013 21:20
4/28/2013 21:30
4/28/2013 21:40
4/28/2013 21:50
10.52
10.51
10.5
10.49
10.49
10.48
10.47
10.46
10.46
10.46
10.45
10.45
10.44
10.44
10.43
10.43
10.44
10.45
10.46
10.49
10.51
10.54
10.57
10.59
10.62
13.24
13.23
13.22
13.22
13.21
13.21
9.43
9.45
9.41
9.4
9.45
9.41
9.41
9.4
9.41
9.4
9.41
9.38
9.41
9.41
9.43
9.4
9.4
9.48
9.49
9.48
9.52
9.54
9.59
9.63
9.7
8.97
8.94
8.86
8.83
8.82
8.83
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.05
9.05
5/30/2013 1:40
5/30/2013 1:50
5/30/2013 2:00
5/30/2013 2:10
5/30/2013 2:20
5/30/2013 2:30
5/30/2013 2:40
5/30/2013 2:50
5/30/2013 3:00
5/30/2013 3:10
5/30/2013 3:20
5/30/2013 3:30
5/30/2013 3:40
5/30/2013 3:50
5/30/2013 4:00
5/30/2013 4:10
5/30/2013 4:20
5/30/2013 4:30
5/30/2013 4:40
5/30/2013 4:50
5/30/2013 5:00
5/30/2013 5:10
5/30/2013 5:20
5/30/2013 5:30
5/30/2013 5:40
5/30/2013 5:50
5/30/2013 6:00
5/30/2013 6:10
5/30/2013 6:20
5/30/2013 6:30
5/30/2013 6:40
21.31
21.28
21.25
21.23
21.2
21.17
21.13
21.11
21.07
21.04
21.02
20.99
20.96
20.93
20.9
20.88
20.85
20.82
20.8
20.77
20.75
20.74
20.71
20.69
20.67
20.66
20.64
20.63
20.62
20.61
20.62
82
7.08
7.07
7.04
7.01
6.99
7
6.97
7
6.95
6.95
6.91
6.95
6.92
6.9
6.9
6.91
6.87
6.91
6.87
6.87
6.85
6.85
6.82
6.86
6.84
6.85
6.87
6.86
6.89
6.89
6.91
8.74
8.73
8.73
8.73
8.73
8.73
8.73
8.73
8.72
8.73
8.73
8.73
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.72
8.73
8.73
8.73
6/17/2013 20:50
6/17/2013 21:00
6/17/2013 21:10
6/17/2013 21:20
6/17/2013 21:30
6/17/2013 21:40
6/17/2013 21:50
6/17/2013 22:00
6/17/2013 22:10
6/17/2013 22:20
6/17/2013 22:30
6/17/2013 22:40
6/17/2013 22:50
6/17/2013 23:00
6/17/2013 23:10
6/17/2013 23:20
6/17/2013 23:30
6/17/2013 23:40
6/17/2013 23:50
6/18/2013 0:00
6/18/2013 0:10
6/18/2013 0:20
6/18/2013 0:30
6/18/2013 0:40
6/18/2013 0:50
6/18/2013 1:00
6/18/2013 1:10
6/18/2013 1:20
6/18/2013 1:30
6/18/2013 1:40
6/18/2013 1:50
23.51
23.51
23.5
23.5
23.5
23.5
23.5
23.49
23.48
23.47
23.45
23.43
23.4
23.38
23.35
23.32
23.28
23.25
23.2
23.17
23.12
23.08
23.04
23.01
22.97
22.93
22.89
22.85
22.81
22.77
22.73
6.97
6.96
6.91
6.84
6.85
6.81
6.78
6.75
6.71
6.69
6.65
6.65
6.63
6.61
6.61
6.57
6.58
6.52
6.52
6.49
6.48
6.48
6.44
6.44
6.42
6.43
6.41
6.42
6.41
6.38
6.41
9.01
9.01
9.01
9.01
9.01
9.01
9.01
9
9
9
9
9
9
8.99
8.99
8.99
8.99
8.98
8.98
8.98
8.98
8.98
8.98
8.98
8.98
8.98
8.98
8.98
8.97
8.97
8.97
4/28/2013 22:00
4/28/2013 22:10
4/28/2013 22:20
4/28/2013 22:30
4/28/2013 22:40
4/28/2013 22:50
4/28/2013 23:00
4/28/2013 23:10
4/28/2013 23:20
4/28/2013 23:30
4/28/2013 23:40
4/28/2013 23:50
4/29/2013 0:00
4/29/2013 0:10
4/29/2013 0:20
4/29/2013 0:30
4/29/2013 0:40
4/29/2013 0:50
4/29/2013 1:00
4/29/2013 1:10
4/29/2013 1:20
4/29/2013 1:30
4/29/2013 1:40
4/29/2013 1:50
4/29/2013 2:00
4/29/2013 2:10
4/29/2013 2:20
4/29/2013 2:30
4/29/2013 2:40
4/29/2013 2:50
4/29/2013 3:00
13.2
13.2
13.19
13.18
13.18
13.18
13.18
13.17
13.17
13.16
13.16
13.15
13.14
13.14
13.13
13.12
13.11
13.1
13.09
13.08
13.08
13.07
13.06
13.05
13.04
13.04
13.02
13.02
13.01
13
12.99
8.79
8.72
8.7
8.66
8.68
8.65
8.59
8.59
8.58
8.55
8.56
8.52
8.52
8.5
8.47
8.48
8.43
8.44
8.42
8.42
8.42
8.39
8.38
8.38
8.39
8.36
8.35
8.37
8.39
8.37
8.36
9.04
9.04
9.03
9.04
9.04
9.04
9.04
9.05
9.05
9.05
9.05
9.05
9.06
9.06
9.06
9.06
9.07
9.07
9.08
9.09
9.09
9.08
9.08
9.08
9.08
9.09
9.09
9.1
9.1
9.1
9.11
5/30/2013 6:50
5/30/2013 7:00
5/30/2013 7:10
5/30/2013 7:20
5/30/2013 7:30
5/30/2013 7:40
5/30/2013 7:50
5/30/2013 8:00
5/30/2013 8:10
5/30/2013 8:20
5/30/2013 8:30
5/30/2013 8:40
5/30/2013 8:50
5/30/2013 9:00
5/30/2013 9:10
5/30/2013 9:20
5/30/2013 9:30
5/30/2013 9:40
5/30/2013 9:50
5/30/2013 10:00
5/30/2013 10:10
5/30/2013 10:20
5/30/2013 10:30
5/30/2013 10:40
5/30/2013 10:50
5/30/2013 11:00
5/30/2013 11:10
5/30/2013 11:20
5/30/2013 11:30
5/30/2013 11:40
5/30/2013 11:50
20.62
20.62
20.63
20.63
20.63
20.64
20.64
20.66
20.68
20.7
20.73
20.77
20.81
20.86
20.92
20.98
21.06
21.14
21.2
21.31
21.38
21.48
21.58
21.65
21.75
21.83
21.95
22.05
22.2
22.33
22.44
83
6.95
6.96
6.98
7.02
7.06
7.1
7.19
7.24
7.38
7.44
7.5
7.58
7.61
7.72
7.81
7.96
8.09
8.15
8.25
8.37
8.5
8.62
8.78
8.84
9
9.07
9.28
9.35
9.52
9.62
9.75
8.73
8.73
8.74
8.74
8.74
8.74
8.74
8.75
8.75
8.76
8.76
8.77
8.77
8.77
8.78
8.78
8.79
8.8
8.81
8.82
8.83
8.83
8.84
8.85
8.86
8.87
8.88
8.88
8.89
8.9
8.91
6/18/2013 2:00
6/18/2013 2:10
6/18/2013 2:20
6/18/2013 2:30
6/18/2013 2:40
6/18/2013 2:50
6/18/2013 3:00
6/18/2013 3:10
6/18/2013 3:20
6/18/2013 3:30
6/18/2013 3:40
6/18/2013 3:50
6/18/2013 4:00
6/18/2013 4:10
6/18/2013 4:20
6/18/2013 4:30
6/18/2013 4:40
6/18/2013 4:50
6/18/2013 5:00
6/18/2013 5:10
6/18/2013 5:20
6/18/2013 5:30
6/18/2013 5:40
6/18/2013 5:50
6/18/2013 6:00
6/18/2013 6:10
6/18/2013 6:20
6/18/2013 6:30
6/18/2013 6:40
6/18/2013 6:50
6/18/2013 7:00
22.69
22.66
22.63
22.6
22.57
22.54
22.51
22.48
22.46
22.45
22.44
22.44
22.42
22.38
22.33
22.28
22.23
22.19
22.16
22.12
22.09
22.06
22.04
22.02
21.99
21.97
21.94
21.92
21.89
21.87
21.85
6.41
6.42
6.4
6.39
6.41
6.39
6.4
6.39
6.37
6.39
6.38
6.4
6.37
6.37
6.39
6.39
6.39
6.4
6.39
6.41
6.41
6.4
6.42
6.44
6.39
6.41
6.43
6.41
6.41
6.43
6.46
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.98
8.98
8.98
8.98
8.98
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.97
8.98
8.98
8.98
8.98
4/29/2013 3:10
4/29/2013 3:20
4/29/2013 3:30
4/29/2013 3:40
4/29/2013 3:50
4/29/2013 4:00
4/29/2013 4:10
4/29/2013 4:20
4/29/2013 4:30
4/29/2013 4:40
4/29/2013 4:50
4/29/2013 5:00
4/29/2013 5:10
4/29/2013 5:20
4/29/2013 5:30
4/29/2013 5:40
4/29/2013 5:50
4/29/2013 6:00
4/29/2013 6:10
4/29/2013 6:20
4/29/2013 6:30
4/29/2013 6:40
4/29/2013 6:50
4/29/2013 7:00
4/29/2013 7:10
4/29/2013 7:20
4/29/2013 7:30
4/29/2013 7:40
4/29/2013 7:50
4/29/2013 8:00
4/29/2013 8:10
12.98
12.97
12.96
12.95
12.94
12.93
12.92
12.91
12.9
12.9
12.89
12.88
12.87
12.87
12.86
12.85
12.85
12.84
12.83
12.83
12.82
12.82
12.82
12.81
12.81
12.8
12.8
12.8
12.8
12.8
12.8
8.37
8.4
8.36
8.36
8.37
8.37
8.39
8.41
8.4
8.38
8.39
8.37
8.39
8.4
8.38
8.39
8.42
8.38
8.42
8.39
8.42
8.44
8.43
8.44
8.48
8.42
8.44
8.48
8.5
8.47
8.48
9.11
9.11
9.11
9.11
9.11
9.11
9.11
9.11
9.11
9.12
9.12
9.12
9.12
9.12
9.12
9.12
9.13
9.14
9.13
9.13
9.14
9.14
9.14
9.15
9.14
9.14
9.14
9.14
9.14
9.14
9.14
5/30/2013 12:00
5/30/2013 12:10
5/30/2013 12:20
5/30/2013 12:30
5/30/2013 12:40
5/30/2013 12:50
5/30/2013 13:00
5/30/2013 13:10
5/30/2013 13:20
5/30/2013 13:30
5/30/2013 13:40
5/30/2013 13:50
5/30/2013 14:00
5/30/2013 14:10
5/30/2013 14:20
5/30/2013 14:30
5/30/2013 14:40
5/30/2013 14:50
5/30/2013 15:00
5/30/2013 15:10
5/30/2013 15:20
5/30/2013 15:30
5/30/2013 15:40
5/30/2013 15:50
5/30/2013 16:00
5/30/2013 16:10
5/30/2013 16:20
5/30/2013 16:30
5/30/2013 16:40
5/30/2013 16:50
5/30/2013 17:00
22.53
22.62
22.77
22.87
23.02
23.15
23.21
23.28
23.27
23.41
23.55
23.6
23.67
23.72
23.73
23.82
23.91
23.99
24.05
24.05
24.1
24.12
24.1
24.1
24.12
24.15
24.18
24.16
24.14
24.13
24.12
84
9.79
9.93
10.07
10.14
10.34
10.42
10.44
10.48
10.33
10.55
10.72
10.76
10.79
10.78
10.68
10.75
10.91
10.99
11
10.82
10.76
10.71
10.64
10.49
10.47
10.49
10.48
10.45
10.33
10.23
10.13
8.92
8.93
8.93
8.94
8.95
8.96
8.96
8.97
8.97
8.98
9
9
9.01
9.01
9
9.01
9.02
9.02
9.02
9.02
9.03
9.02
9.01
9.01
9.01
9.01
9.02
9.02
9.02
9.01
9.01
6/18/2013 7:10
6/18/2013 7:20
6/18/2013 7:30
6/18/2013 7:40
6/18/2013 7:50
6/18/2013 8:00
6/18/2013 8:10
6/18/2013 8:20
6/18/2013 8:30
6/18/2013 8:40
6/18/2013 8:50
6/18/2013 9:00
6/18/2013 9:10
6/18/2013 9:20
6/18/2013 9:30
6/18/2013 9:40
6/18/2013 9:50
6/18/2013 10:00
6/18/2013 10:10
6/18/2013 10:20
6/18/2013 10:30
6/18/2013 10:40
6/18/2013 10:50
6/18/2013 11:00
6/18/2013 11:10
6/18/2013 11:20
6/18/2013 11:30
6/18/2013 11:40
6/18/2013 11:50
6/18/2013 12:00
6/18/2013 12:10
21.83
21.82
21.79
21.78
21.77
21.77
21.77
21.78
21.77
21.78
21.8
21.81
21.83
21.84
21.84
21.85
21.91
21.95
22.01
22.07
22.13
22.19
22.27
22.34
22.42
22.52
22.61
22.71
22.81
22.9
23
6.45
6.49
6.51
6.58
6.57
6.61
6.61
6.68
6.71
6.72
6.8
6.81
6.9
6.96
6.89
6.91
6.98
7.04
7.16
7.12
7.2
7.3
7.38
7.45
7.44
7.5
7.53
7.6
7.61
7.72
7.9
8.98
8.98
8.98
8.98
8.99
8.98
8.99
8.99
8.99
8.99
8.99
9
9
9
9.01
9.01
9.01
9.02
9.02
9.03
9.03
9.04
9.04
9.05
9.06
9.06
9.06
9.07
9.07
9.07
9.08
4/29/2013 8:20
4/29/2013 8:30
4/29/2013 8:40
4/29/2013 8:50
4/29/2013 9:00
4/29/2013 9:10
4/29/2013 9:20
4/29/2013 9:30
4/29/2013 9:40
4/29/2013 9:50
4/29/2013 10:00
4/29/2013 10:10
4/29/2013 10:20
4/29/2013 10:30
4/29/2013 10:40
4/29/2013 10:50
4/29/2013 11:00
4/29/2013 11:10
4/29/2013 11:20
4/29/2013 11:30
4/29/2013 11:40
4/29/2013 11:50
4/29/2013 12:00
4/29/2013 12:10
4/29/2013 12:20
4/29/2013 12:30
4/29/2013 12:40
4/29/2013 12:50
4/29/2013 13:00
4/29/2013 13:10
4/29/2013 13:20
12.8
12.8
12.81
12.81
12.82
12.84
12.85
12.86
12.88
12.9
12.91
12.93
12.95
12.97
12.99
13.02
13.06
13.09
13.12
13.17
13.21
13.25
13.3
13.34
13.39
13.43
13.45
13.47
13.49
13.51
13.54
8.52
8.54
8.55
8.57
8.58
8.61
8.68
8.69
8.72
8.78
8.81
8.83
8.85
8.86
8.92
9
9.04
9.08
9.14
9.16
9.21
9.27
9.28
9.32
9.35
9.34
9.34
9.33
9.34
9.34
9.37
9.14
9.14
9.14
9.14
9.14
9.15
9.15
9.14
9.13
9.12
9.12
9.11
9.11
9.11
9.11
9.11
9.11
9.11
9.1
9.1
9.1
9.09
9.09
9.09
9.09
9.08
9.08
9.08
9.08
9.08
9.08
5/30/2013 17:10
5/30/2013 17:20
5/30/2013 17:30
5/30/2013 17:40
5/30/2013 17:50
5/30/2013 18:00
5/30/2013 18:10
5/30/2013 18:20
5/30/2013 18:30
5/30/2013 18:40
5/30/2013 18:50
5/30/2013 19:00
5/30/2013 19:10
5/30/2013 19:20
5/30/2013 19:30
5/30/2013 19:40
5/30/2013 19:50
5/30/2013 20:00
5/30/2013 20:10
5/30/2013 20:20
5/30/2013 20:30
5/30/2013 20:40
5/30/2013 20:50
5/30/2013 21:00
5/30/2013 21:10
5/30/2013 21:20
5/30/2013 21:30
5/30/2013 21:40
5/30/2013 21:50
5/30/2013 22:00
5/30/2013 22:10
24.12 10.04
24.02 9.87
23.97 9.71
23.94 9.51
23.9 9.44
23.89 9.43
23.86 9.42
23.86 9.43
23.81 8.37
23.77
9.1
23.72 9.01
23.66 8.86
23.61 8.69
23.57 8.65
23.53 8.59
23.5
8.5
23.46 8.39
23.41 8.28
23.38 8.16
23.33 8.08
23.29 7.99
23.25 7.92
23.21 7.86
23.17 7.81
23.14 7.72
23.11 7.63
23.07 7.56
23.03 7.49
23.01 7.37
22.99 7.36
22.96 7.24
85
9.01
9
8.99
8.99
8.99
8.99
8.98
8.98
8.97
8.96
8.96
8.95
8.94
8.93
8.93
8.93
8.92
8.91
8.91
8.9
8.9
8.89
8.89
8.89
8.88
8.88
8.88
8.87
8.86
8.86
8.85
6/18/2013 12:20
6/18/2013 12:30
6/18/2013 12:40
6/18/2013 12:50
6/18/2013 13:00
6/18/2013 13:10
6/18/2013 13:20
6/18/2013 13:30
6/18/2013 13:40
6/18/2013 13:50
6/18/2013 14:00
6/18/2013 14:10
6/18/2013 14:20
6/18/2013 14:30
6/18/2013 14:40
6/18/2013 14:50
6/18/2013 15:00
6/18/2013 15:10
6/18/2013 15:20
6/18/2013 15:30
6/18/2013 15:40
6/18/2013 15:50
6/18/2013 16:00
6/18/2013 16:10
6/18/2013 16:20
6/18/2013 16:30
6/18/2013 16:40
6/18/2013 16:50
6/18/2013 17:00
6/18/2013 17:10
6/18/2013 17:20
23.09
23.18
23.26
23.36
23.45
23.54
23.65
23.73
23.82
23.88
23.91
24.05
24.11
24.2
24.26
24.3
24.4
24.49
24.59
24.68
24.75
24.8
24.84
24.83
24.84
24.82
24.8
24.78
24.74
24.7
24.68
7.91
7.94
7.88
8.03
9.08
9.08
9.08
9.08
9.09
9.09
9.09
9.1
9.1
9.1
9.11
9.11
9.12
9.12
9.12
9.12
9.13
9.13
9.13
9.13
9.14
9.14
9.14
9.14
9.14
9.14
9.14
9.14
9.13
9.13
9.13
4/29/2013 13:30
4/29/2013 13:40
4/29/2013 13:50
4/29/2013 14:00
4/29/2013 14:10
4/29/2013 14:20
4/29/2013 14:30
4/29/2013 14:40
4/29/2013 14:50
4/29/2013 15:00
4/29/2013 15:10
4/29/2013 15:20
4/29/2013 15:30
4/29/2013 15:40
4/29/2013 15:50
4/29/2013 16:00
4/29/2013 16:10
4/29/2013 16:20
4/29/2013 16:30
4/29/2013 16:40
4/29/2013 16:50
4/29/2013 17:00
4/29/2013 17:10
4/29/2013 17:20
4/29/2013 17:30
4/29/2013 17:40
4/29/2013 17:50
4/29/2013 18:00
4/29/2013 18:10
4/29/2013 18:20
4/29/2013 18:30
13.57
13.61
13.65
13.71
13.75
13.8
13.86
13.91
14
14.06
14.09
14.11
14.21
14.26
14.3
14.37
14.38
14.34
14.35
14.36
14.41
14.45
14.49
14.51
14.53
14.55
14.58
14.58
14.61
14.62
14.62
9.39
9.37
9.4
9.42
9.43
9.39
9.45
9.49
9.54
9.59
9.5
9.51
9.54
9.58
9.54
9.59
9.6
9.54
9.51
9.47
9.5
9.47
9.49
9.45
9.41
9.35
9.43
9.34
9.33
9.3
9.25
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.08
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.07
9.08
9.08
9.07
9.06
9.06
9.05
9.05
5/30/2013 22:20
5/30/2013 22:30
5/30/2013 22:40
5/30/2013 22:50
5/30/2013 23:00
5/30/2013 23:10
5/30/2013 23:20
5/30/2013 23:30
5/30/2013 23:40
5/30/2013 23:50
5/31/2013 0:00
5/31/2013 0:10
5/31/2013 0:20
5/31/2013 0:30
5/31/2013 0:40
5/31/2013 0:50
5/31/2013 1:00
5/31/2013 1:10
5/31/2013 1:20
5/31/2013 1:30
5/31/2013 1:40
5/31/2013 1:50
5/31/2013 2:00
5/31/2013 2:10
5/31/2013 2:20
5/31/2013 2:30
5/31/2013 2:40
5/31/2013 2:50
5/31/2013 3:00
5/31/2013 3:10
5/31/2013 3:20
22.93
22.9
22.88
22.84
22.81
22.78
22.75
22.74
22.71
22.7
22.68
22.66
22.62
22.59
22.55
22.52
22.48
22.44
22.41
22.38
22.34
22.31
22.28
22.24
22.21
22.17
22.14
22.11
22.08
22.04
22.01
86
7.19
7.14
7.12
7.06
7.03
6.99
6.98
6.96
6.9
6.87
6.89
6.87
6.82
6.8
6.78
6.76
6.78
6.73
6.75
6.71
6.72
6.7
6.69
6.7
6.7
6.68
6.64
6.63
6.64
6.63
6.64
8.85
8.85
8.84
8.84
8.84
8.84
8.84
8.84
8.84
8.84
8.84
8.84
8.84
8.83
8.83
8.83
8.83
8.83
8.83
8.83
8.83
8.83
8.83
8.83
8.82
8.83
8.82
8.82
8.82
8.82
8.82
6/18/2013 17:30
6/18/2013 17:40
6/18/2013 17:50
6/18/2013 18:00
6/18/2013 18:10
6/18/2013 18:20
6/18/2013 18:30
6/18/2013 18:40
6/18/2013 18:50
6/18/2013 19:00
6/18/2013 19:10
6/18/2013 19:20
6/18/2013 19:30
6/18/2013 19:40
6/18/2013 19:50
6/18/2013 20:00
6/18/2013 20:10
6/18/2013 20:20
6/18/2013 20:30
6/18/2013 20:40
6/18/2013 20:50
6/18/2013 21:00
6/18/2013 21:10
6/18/2013 21:20
6/18/2013 21:30
6/18/2013 21:40
6/18/2013 21:50
6/18/2013 22:00
6/18/2013 22:10
6/18/2013 22:20
6/18/2013 22:30
24.64
24.6
24.56
24.52
24.49
24.46
24.44
24.42
24.4
24.37
24.37
24.35
24.32
24.28
24.24
24.19
24.15
24.09
24.05
24.02
23.99
23.96
23.93
23.89
23.86
23.84
23.81
23.78
23.75
23.72
23.69
9.12
9.12
9.12
9.12
9.12
9.12
9.11
9.11
9.11
9.11
9.11
9.11
9.1
9.09
9.09
9.08
9.08
9.08
9.07
9.07
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.05
9.05
4/29/2013 18:40
4/29/2013 18:50
4/29/2013 19:00
4/29/2013 19:10
4/29/2013 19:20
4/29/2013 19:30
4/29/2013 19:40
4/29/2013 19:50
4/29/2013 20:00
4/29/2013 20:10
4/29/2013 20:20
4/29/2013 20:30
4/29/2013 20:40
4/29/2013 20:50
4/29/2013 21:00
4/29/2013 21:10
14.62
14.62
14.62
14.62
14.63
14.63
14.63
14.63
14.62
14.6
14.57
14.53
14.49
14.46
14.42
14.39
9.19
9.18
9.14
9.11
9.09
9.04
9.01
8.95
8.9
8.89
8.85
8.83
8.74
8.71
8.66
8.63
9.05
9.05
9.05
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.06
9.07
9.07
9.06
DO
8.24
8.22
8.16
8.2
8.1
8.07
7.98
7.91
7.88
7.84
7.78
pH
9.25
9.25
9.25
9.25
9.25
9.25
9.24
9.24
9.23
9.23
9.23
5/31/2013 3:30
5/31/2013 3:40
5/31/2013 3:50
5/31/2013 4:00
5/31/2013 4:10
5/31/2013 4:20
5/31/2013 4:30
5/31/2013 4:40
5/31/2013 4:50
5/31/2013 5:00
5/31/2013 5:10
5/31/2013 5:20
5/31/2013 5:30
5/31/2013 5:40
5/31/2013 5:50
5/31/2013 6:00
21.97
21.94
21.91
21.88
21.85
21.82
21.79
21.76
21.74
21.71
21.68
21.66
21.64
21.62
21.61
21.6
6.66
6.62
6.64
6.63
6.63
6.57
6.63
6.58
6.61
6.6
6.62
6.61
6.64
6.59
6.62
6.63
8.83
8.82
8.82
8.82
8.82
8.82
8.82
8.82
8.82
8.82
8.82
8.82
8.82
8.83
8.83
8.83
6/18/2013 22:40
6/18/2013 22:50
6/18/2013 23:00
6/18/2013 23:10
6/18/2013 23:20
6/18/2013 23:30
6/18/2013 23:40
6/18/2013 23:50
6/19/2013 0:00
6/19/2013 0:10
6/19/2013 0:20
6/19/2013 0:30
6/19/2013 0:40
6/19/2013 0:50
6/19/2013 1:00
6/19/2013 1:10
23.67
23.65
23.62
23.59
23.57
23.54
23.51
23.47
23.44
23.41
23.36
23.33
23.29
23.24
23.2
23.15
9.05
9.05
9.05
9.05
9.05
9.05
9.05
9.05
9.05
9.05
9.04
9.04
9.04
9.04
9.04
9.04
Appendix 1. (continued)
July
7/26/2013 16:40
7/26/2013 16:50
7/26/2013 17:00
7/26/2013 17:10
7/26/2013 17:20
7/26/2013 17:30
7/26/2013 17:40
7/26/2013 17:50
7/26/2013 18:00
7/26/2013 18:10
7/26/2013 18:20
Temp
24.53
24.56
24.55
24.56
24.57
24.57
24.58
24.57
24.58
24.58
24.56
August
Temp
8/6/2013 18:00 22.72
8/6/2013 18:10 22.72
8/6/2013 18:20 22.71
8/6/2013 18:30 22.71
8/6/2013 18:40 22.71
8/6/2013 18:50 22.71
8/6/2013 19:00 22.73
8/6/2013 19:10 22.74
8/6/2013 19:20 22.74
8/6/2013 19:30 22.74
8/6/2013 19:40 22.74
87
DO
9.8
9.69
9.68
9.58
9.52
9.5
9.47
9.46
9.43
9.3
9.26
pH
9.11
9.11
9.11
9.1
9.1
9.1
9.1
9.1
9.1
9.09
9.09
September
9/11/2013 21:00
9/11/2013 21:10
9/11/2013 21:20
9/11/2013 21:30
9/11/2013 21:40
9/11/2013 21:50
9/11/2013 22:00
9/11/2013 22:10
9/11/2013 22:20
9/11/2013 22:30
9/11/2013 22:40
Temp
25.83
25.79
25.75
25.7
25.66
25.61
25.56
25.51
25.46
25.43
25.38
DO
6.35
6.29
6.22
6.14
6.06
5.99
5.92
5.84
5.76
5.71
5.62
pH
9.05
9.04
9.04
9.03
9.02
9.01
9.01
9
8.99
8.99
8.98
7/26/2013 18:30
7/26/2013 18:40
7/26/2013 18:50
7/26/2013 19:00
7/26/2013 19:10
7/26/2013 19:20
7/26/2013 19:30
7/26/2013 19:40
7/26/2013 19:50
7/26/2013 20:00
7/26/2013 20:10
7/26/2013 20:20
7/26/2013 20:30
7/26/2013 20:40
7/26/2013 20:50
7/26/2013 21:00
7/26/2013 21:10
7/26/2013 21:20
7/26/2013 21:30
7/26/2013 21:40
7/26/2013 21:50
7/26/2013 22:00
7/26/2013 22:10
7/26/2013 22:20
7/26/2013 22:30
7/26/2013 22:40
7/26/2013 22:50
7/26/2013 23:00
7/26/2013 23:10
7/26/2013 23:20
7/26/2013 23:30
24.56
24.54
24.52
24.5
24.47
24.44
24.42
24.39
24.36
24.33
24.29
24.26
24.23
24.19
24.16
24.12
24.08
24.05
24.01
23.97
23.93
23.9
23.87
23.83
23.8
23.76
23.72
23.69
23.65
23.62
23.57
7.76
7.67
7.6
7.54
7.47
7.35
7.29
7.23
7.14
7.07
7
6.92
6.84
6.79
6.69
6.59
6.53
6.52
6.41
6.32
6.25
6.18
6.12
6.07
6.01
5.92
5.91
5.84
5.78
5.73
5.68
9.22
9.21
9.21
9.2
9.2
9.2
9.19
9.19
9.18
9.17
9.17
9.16
9.16
9.15
9.14
9.14
9.13
9.13
9.12
9.11
9.11
9.1
9.1
9.09
9.09
9.08
9.07
9.07
9.06
9.06
9.05
8/6/2013 19:50
8/6/2013 20:00
8/6/2013 20:10
8/6/2013 20:20
8/6/2013 20:30
8/6/2013 20:40
8/6/2013 20:50
8/6/2013 21:00
8/6/2013 21:10
8/6/2013 21:20
8/6/2013 21:30
8/6/2013 21:40
8/6/2013 21:50
8/6/2013 22:00
8/6/2013 22:10
8/6/2013 22:20
8/6/2013 22:30
8/6/2013 22:40
8/6/2013 22:50
8/6/2013 23:00
8/6/2013 23:10
8/6/2013 23:20
8/6/2013 23:30
8/6/2013 23:40
8/6/2013 23:50
8/7/2013 0:00
8/7/2013 0:10
8/7/2013 0:20
8/7/2013 0:30
8/7/2013 0:40
8/7/2013 0:50
22.72
22.7
22.68
22.65
22.63
22.6
22.58
22.55
22.53
22.5
22.48
22.46
22.43
22.41
22.38
22.36
22.34
22.32
22.3
22.27
22.24
22.22
22.2
22.18
22.16
22.14
22.12
22.1
22.08
22.06
22.04
88
9.22
9.12
9.06
8.98
8.87
8.75
8.72
8.62
8.55
8.43
8.39
8.34
8.23
8.18
8.09
8.03
7.99
7.89
7.86
7.76
7.73
7.66
7.61
7.56
7.48
7.46
7.41
7.33
7.32
7.26
7.22
9.09
9.08
9.08
9.07
9.07
9.06
9.05
9.05
9.04
9.03
9.03
9.03
9.02
9.01
9.01
9
9
8.99
8.99
8.99
8.98
8.98
8.97
8.96
8.96
8.95
8.95
8.95
8.95
8.94
8.93
9/11/2013 22:50
9/11/2013 23:00
9/11/2013 23:10
9/11/2013 23:20
9/11/2013 23:30
9/11/2013 23:40
9/11/2013 23:50
9/12/2013 0:00
9/12/2013 0:10
9/12/2013 0:20
9/12/2013 0:30
9/12/2013 0:40
9/12/2013 0:50
9/12/2013 1:00
9/12/2013 1:10
9/12/2013 1:20
9/12/2013 1:30
9/12/2013 1:40
9/12/2013 1:50
9/12/2013 2:00
9/12/2013 2:10
9/12/2013 2:20
9/12/2013 2:30
9/12/2013 2:40
9/12/2013 2:50
9/12/2013 3:00
9/12/2013 3:10
9/12/2013 3:20
9/12/2013 3:30
9/12/2013 3:40
9/12/2013 3:50
25.34
25.3
25.25
25.2
25.16
25.12
25.08
25.04
24.98
24.95
24.91
24.87
24.83
24.8
24.76
24.73
24.69
24.66
24.63
24.6
24.56
24.53
24.49
24.46
24.42
24.39
24.35
24.32
24.28
24.25
24.21
5.56
5.49
5.44
5.36
5.34
5.27
5.26
5.18
5.15
5.1
5.05
5.02
4.99
4.96
4.92
4.89
4.85
4.82
4.78
4.75
4.73
4.69
4.67
4.65
4.62
4.6
4.57
4.56
4.53
4.49
4.49
8.98
8.97
8.97
8.96
8.96
8.95
8.95
8.95
8.94
8.94
8.93
8.93
8.93
8.92
8.92
8.92
8.92
8.92
8.91
8.91
8.91
8.91
8.9
8.9
8.9
8.9
8.9
8.9
8.89
8.89
8.89
7/26/2013 23:40
7/26/2013 23:50
7/27/2013 0:00
7/27/2013 0:10
7/27/2013 0:20
7/27/2013 0:30
7/27/2013 0:40
7/27/2013 0:50
7/27/2013 1:00
7/27/2013 1:10
7/27/2013 1:20
7/27/2013 1:30
7/27/2013 1:40
7/27/2013 1:50
7/27/2013 2:00
7/27/2013 2:10
7/27/2013 2:20
7/27/2013 2:30
7/27/2013 2:40
7/27/2013 2:50
7/27/2013 3:00
7/27/2013 3:10
7/27/2013 3:20
7/27/2013 3:30
7/27/2013 3:40
7/27/2013 3:50
7/27/2013 4:00
7/27/2013 4:10
7/27/2013 4:20
7/27/2013 4:30
7/27/2013 4:40
23.54
23.5
23.46
23.41
23.38
23.33
23.29
23.26
23.22
23.17
23.13
23.08
23.04
23
22.95
22.91
22.87
22.84
22.81
22.77
22.73
22.71
22.67
22.64
22.6
22.57
22.53
22.5
22.47
22.43
22.39
5.62
5.59
5.54
5.5
5.46
5.41
5.38
5.35
5.31
5.29
5.26
5.22
5.2
5.17
5.13
5.11
5.1
5.07
5.05
5.04
5
4.99
4.97
4.98
4.93
4.93
4.92
4.87
4.87
4.86
4.85
9.05
9.05
9.04
9.03
9.03
9.03
9.02
9.02
9.01
9.01
9
9
9
9
8.99
8.99
8.99
8.99
8.98
8.98
8.97
8.97
8.97
8.96
8.96
8.96
8.96
8.95
8.95
8.95
8.95
8/7/2013 1:00
8/7/2013 1:10
8/7/2013 1:20
8/16/2013 15:30
8/16/2013 15:40
8/16/2013 15:50
8/16/2013 16:00
8/16/2013 16:10
8/16/2013 16:20
8/16/2013 16:30
8/16/2013 16:40
8/16/2013 16:50
8/16/2013 17:00
8/16/2013 17:10
8/16/2013 17:20
8/16/2013 17:30
8/16/2013 17:40
8/16/2013 17:50
8/16/2013 18:00
8/16/2013 18:10
8/16/2013 18:20
8/16/2013 18:30
8/16/2013 18:40
8/16/2013 18:50
8/16/2013 19:00
8/16/2013 19:10
8/16/2013 19:20
8/16/2013 19:30
8/16/2013 19:40
8/16/2013 19:50
8/16/2013 20:00
22.02
22
21.97
21.21
21.21
21.21
21.24
21.25
21.25
21.26
21.28
21.31
21.34
21.36
21.41
21.44
21.45
21.45
21.46
21.46
21.46
21.45
21.44
21.42
21.4
21.38
21.37
21.35
21.32
21.29
21.26
89
7.18
7.14
7.08
8.54
8.53
8.49
8.52
8.5
8.47
8.47
8.48
8.47
8.46
8.43
8.5
8.52
8.48
8.48
8.45
8.39
8.32
8.25
8.14
8.14
8.04
7.99
7.89
7.82
7.74
7.67
7.56
8.93
8.93
8.92
8.92
8.92
8.92
8.91
8.91
8.91
8.91
8.9
8.9
8.9
8.9
8.89
8.89
8.89
8.89
8.88
8.88
8.88
8.87
8.87
8.87
8.87
8.87
8.87
8.86
8.86
8.86
8.86
9/12/2013 4:00
9/12/2013 4:10
9/12/2013 4:20
9/12/2013 4:30
9/12/2013 4:40
9/12/2013 4:50
9/12/2013 5:00
9/12/2013 5:10
9/12/2013 5:20
9/12/2013 5:30
9/12/2013 5:40
9/12/2013 5:50
9/12/2013 6:00
9/12/2013 6:10
9/12/2013 6:20
9/12/2013 6:30
9/12/2013 6:40
9/12/2013 6:50
9/12/2013 7:00
9/12/2013 7:10
9/12/2013 7:20
9/12/2013 7:30
9/12/2013 7:40
9/12/2013 7:50
9/12/2013 8:00
9/12/2013 8:10
9/12/2013 8:20
9/12/2013 8:30
9/12/2013 8:40
9/12/2013 8:50
9/12/2013 9:00
24.18
24.15
24.12
24.08
24.06
24.03
23.99
23.96
23.94
23.91
23.88
23.85
23.71
23.66
23.56
23.52
23.5
23.47
23.45
23.43
23.41
23.37
23.33
23.3
23.27
23.24
23.2
23.16
23.11
23.05
23.01
4.44
4.43
4.41
4.41
4.36
4.34
4.34
4.32
4.31
4.28
4.28
4.27
4.49
4.54
4.68
4.67
4.65
4.63
4.57
4.53
4.47
4.49
4.5
4.52
4.52
4.54
4.59
4.62
4.66
4.73
4.81
8.89
8.89
8.88
8.88
8.88
8.88
8.88
8.87
8.87
8.87
8.87
8.87
8.87
8.87
8.87
8.87
8.86
8.86
8.86
8.86
8.86
8.86
8.87
8.87
8.87
8.88
8.88
8.88
8.89
8.89
8.89
7/27/2013 4:50
7/27/2013 5:00
7/27/2013 5:10
7/27/2013 5:20
7/27/2013 5:30
7/27/2013 5:40
7/27/2013 5:50
7/27/2013 6:00
7/27/2013 6:10
7/27/2013 6:20
7/27/2013 6:30
7/27/2013 6:40
7/27/2013 6:50
7/27/2013 7:00
7/27/2013 7:10
7/27/2013 7:20
7/27/2013 7:30
7/27/2013 7:40
7/27/2013 7:50
7/27/2013 8:00
7/27/2013 8:10
7/27/2013 8:20
7/27/2013 8:30
7/27/2013 8:40
7/27/2013 8:50
7/27/2013 9:00
7/27/2013 9:10
7/27/2013 9:20
7/27/2013 9:30
7/27/2013 9:40
7/27/2013 9:50
22.37
22.33
22.29
22.25
22.22
22.18
22.15
22.11
22.09
22.06
22.03
22
21.97
21.95
21.92
21.89
21.87
21.85
21.83
21.82
21.8
21.78
21.77
21.76
21.75
21.75
21.74
21.75
21.77
21.81
21.84
4.84
4.83
4.81
4.81
4.82
4.8
4.79
4.75
4.78
4.76
4.76
4.76
4.75
4.75
4.76
4.74
4.74
4.78
4.78
4.79
4.79
4.78
4.84
4.84
4.88
4.93
4.96
5.06
5.15
5.28
5.38
8.94
8.94
8.94
8.94
8.94
8.94
8.94
8.94
8.94
8.94
8.94
8.94
8.95
8.95
8.95
8.95
8.96
8.96
8.97
8.97
8.98
8.98
8.99
8.99
9
9.01
9.01
9.02
9.02
9.04
9.04
8/16/2013 20:10
8/16/2013 20:20
8/16/2013 20:30
8/16/2013 20:40
8/16/2013 20:50
8/16/2013 21:00
8/16/2013 21:10
8/16/2013 21:20
8/16/2013 21:30
8/16/2013 21:40
8/16/2013 21:50
8/16/2013 22:00
8/16/2013 22:10
8/16/2013 22:20
8/16/2013 22:30
8/16/2013 22:40
8/16/2013 22:50
8/16/2013 23:00
8/16/2013 23:10
8/16/2013 23:20
8/16/2013 23:30
8/16/2013 23:40
8/16/2013 23:50
8/17/2013 0:00
8/17/2013 0:10
8/17/2013 0:20
8/17/2013 0:30
8/17/2013 0:40
8/17/2013 0:50
8/17/2013 1:00
8/17/2013 1:10
21.23
21.2
21.17
21.14
21.1
21.08
21.05
21.01
20.98
20.96
20.93
20.9
20.86
20.82
20.79
20.76
20.73
20.7
20.66
20.63
20.6
20.57
20.54
20.51
20.48
20.45
20.42
20.39
20.36
20.33
20.31
90
7.47
7.42
7.32
7.26
7.17
7.12
7.08
7.02
6.97
6.87
6.82
6.76
6.68
6.61
6.58
6.55
6.47
6.43
6.36
6.33
6.25
6.22
6.15
6.1
6.05
6
5.97
5.9
5.88
5.83
5.8
8.86
8.86
8.86
9.24
9.25
9.25
9.25
9.25
9.25
9.26
9.26
9.26
9.26
9.26
9.27
9.27
9.28
9.28
9.28
9.28
9.28
9.27
9.27
9.27
9.27
9.26
9.26
9.25
9.25
9.24
9.23
9/12/2013 9:10
9/12/2013 9:20
9/12/2013 9:30
9/12/2013 9:40
9/12/2013 9:50
9/12/2013 10:00
9/12/2013 10:10
9/12/2013 10:20
9/12/2013 10:30
9/12/2013 10:40
9/12/2013 10:50
9/12/2013 11:00
9/12/2013 11:10
9/12/2013 11:20
9/12/2013 11:30
9/12/2013 11:40
9/12/2013 11:50
9/12/2013 12:00
9/12/2013 12:10
9/12/2013 12:20
9/12/2013 12:30
9/12/2013 12:40
9/12/2013 12:50
9/12/2013 13:00
9/12/2013 13:10
9/12/2013 13:20
9/12/2013 13:30
9/12/2013 13:40
9/12/2013 13:50
9/12/2013 14:00
9/12/2013 14:10
22.98
22.95
22.93
22.88
22.83
22.78
22.74
22.72
22.71
22.7
22.7
22.71
22.73
22.76
22.81
22.88
22.94
22.98
23.03
23.08
23.14
23.19
23.23
23.28
23.33
23.36
23.4
23.45
23.51
23.54
23.56
4.88
4.88
4.89
4.84
4.8
4.8
4.78
4.76
4.73
4.71
4.72
4.72
4.72
4.76
4.81
4.9
4.9
4.9
4.92
4.93
5.01
5.06
5.07
5.14
5.13
5.13
5.1
5.08
5.09
5.04
4.98
8.89
8.9
8.9
8.91
8.92
8.92
8.93
8.94
8.94
8.95
8.95
8.96
8.96
8.97
8.98
8.98
8.99
8.99
9
9.01
9.01
9.02
9.03
9.04
9.05
9.06
9.06
9.07
9.08
9.09
9.1
7/27/2013 10:00
7/27/2013 10:10
7/27/2013 10:20
7/27/2013 10:30
7/27/2013 10:40
7/27/2013 10:50
7/27/2013 11:00
7/27/2013 11:10
7/27/2013 11:20
7/27/2013 11:30
7/27/2013 11:40
7/27/2013 11:50
7/27/2013 12:00
7/27/2013 12:10
7/27/2013 12:20
7/27/2013 12:30
7/27/2013 12:40
7/27/2013 12:50
7/27/2013 13:00
7/27/2013 13:10
7/27/2013 13:20
7/27/2013 13:30
7/27/2013 13:40
7/27/2013 13:50
7/27/2013 14:00
7/27/2013 14:10
7/27/2013 14:20
7/27/2013 14:30
7/27/2013 14:40
7/27/2013 14:50
7/27/2013 15:00
21.85
21.88
21.95
22.03
22.08
22.13
22.17
22.21
22.29
22.44
22.55
22.65
22.79
22.94
23.04
23.12
23.22
23.34
23.37
23.44
23.54
23.65
23.79
23.91
24.03
24.19
24.29
24.45
24.43
24.46
24.49
5.41
5.46
5.56
5.68
5.73
5.8
5.79
5.78
5.86
6.03
6.06
6.15
6.26
6.36
6.49
6.49
6.64
6.7
6.72
6.8
6.9
7
7.13
7.25
7.32
7.43
7.51
7.59
7.57
7.54
7.6
9.05
9.05
9.06
9.06
9.07
9.07
9.08
9.08
9.09
9.09
9.09
9.11
9.11
9.11
9.11
9.12
9.13
9.13
9.13
9.13
9.14
9.14
9.15
9.15
9.16
9.16
9.17
9.17
9.17
9.17
9.17
8/17/2013 1:20
8/17/2013 1:30
8/17/2013 1:40
8/17/2013 1:50
8/17/2013 2:00
8/17/2013 2:10
8/17/2013 2:20
8/17/2013 2:30
8/17/2013 2:40
8/17/2013 2:50
8/17/2013 3:00
8/17/2013 3:10
8/17/2013 3:20
8/17/2013 3:30
8/17/2013 3:40
8/17/2013 3:50
8/17/2013 4:00
8/17/2013 4:10
8/17/2013 4:20
8/17/2013 4:30
8/17/2013 4:40
8/17/2013 4:50
8/17/2013 5:00
8/17/2013 5:10
8/17/2013 5:20
8/17/2013 5:30
8/17/2013 5:40
8/17/2013 5:50
8/17/2013 6:00
8/17/2013 6:10
8/17/2013 6:20
20.28
20.26
20.23
20.2
20.18
20.15
20.11
20.08
20.05
20.01
19.97
19.94
19.91
19.88
19.84
19.81
19.79
19.75
19.73
19.7
19.67
19.64
19.6
19.56
19.53
19.49
19.45
19.41
19.38
19.34
19.31
91
5.77
5.72
5.7
5.65
5.62
5.59
5.56
5.53
5.51
5.48
5.45
5.43
5.39
5.37
5.35
5.31
5.29
5.29
5.27
5.25
5.22
5.21
5.22
5.18
5.17
5.15
5.14
5.14
5.12
5.09
5.07
9.23
9.23
9.22
9.21
9.21
9.2
9.19
9.19
9.18
9.18
9.17
9.16
9.16
9.15
9.14
9.14
9.13
9.12
9.12
9.11
9.11
9.1
9.09
9.09
9.08
9.07
9.07
9.06
9.06
9.05
9.05
9/12/2013 14:20
9/12/2013 14:30
9/12/2013 14:40
9/12/2013 14:50
9/12/2013 15:00
9/12/2013 15:10
9/12/2013 15:20
9/12/2013 15:30
9/12/2013 15:40
9/12/2013 15:50
9/12/2013 16:00
9/12/2013 16:10
9/12/2013 16:20
9/12/2013 16:30
9/12/2013 16:40
9/12/2013 16:50
9/12/2013 17:00
9/12/2013 17:10
9/12/2013 17:20
9/12/2013 17:30
9/12/2013 17:40
9/12/2013 17:50
9/12/2013 18:00
9/12/2013 18:10
9/12/2013 18:20
9/12/2013 18:30
9/12/2013 18:40
9/12/2013 18:50
9/12/2013 19:00
9/12/2013 19:10
9/12/2013 19:20
23.6
23.67
23.73
23.81
23.87
23.9
23.96
23.98
24
24.02
24.04
24.06
24.04
24.04
24.02
24
23.99
23.99
23.98
23.97
23.96
23.94
23.92
23.91
23.9
23.89
23.86
23.82
23.78
23.74
23.69
4.94
4.94
4.9
4.89
4.89
4.87
4.85
4.83
4.81
4.81
4.78
4.79
4.74
4.74
4.72
4.71
4.68
4.69
4.69
4.69
4.7
4.71
4.71
4.73
4.73
4.73
4.73
4.7
4.7
4.64
4.61
9.1
9.11
9.12
9.12
9.14
9.15
9.15
9.16
9.17
9.17
9.17
9.17
9.18
9.17
9.18
9.18
9.19
9.19
9.19
9.19
9.19
9.19
9.19
9.19
9.19
9.18
9.18
9.18
9.17
9.17
9.16
7/27/2013 15:10
7/27/2013 15:20
7/27/2013 15:30
7/27/2013 15:40
7/27/2013 15:50
7/27/2013 16:00
7/27/2013 16:10
7/27/2013 16:20
7/27/2013 16:30
7/27/2013 16:40
7/27/2013 16:50
7/27/2013 17:00
7/27/2013 17:10
7/27/2013 17:20
7/27/2013 17:30
7/27/2013 17:40
7/29/2013 18:00
7/29/2013 18:10
7/29/2013 18:20
7/29/2013 18:30
7/29/2013 18:40
7/29/2013 18:50
7/29/2013 19:00
7/29/2013 19:10
7/29/2013 19:20
7/29/2013 19:30
7/29/2013 19:40
7/29/2013 19:50
7/29/2013 20:00
7/29/2013 20:10
7/29/2013 20:20
24.52
24.48
24.65
24.71
24.7
24.75
24.72
24.7
24.69
24.63
24.62
24.66
24.63
24.65
24.64
24.63
23.57
23.56
23.58
23.57
23.54
23.51
23.47
23.42
23.37
23.32
23.27
23.23
23.17
23.12
23.07
7.57
7.6
7.69
7.7
7.69
7.71
7.7
7.66
7.58
7.52
7.52
7.58
7.52
7.53
7.5
7.43
9.97
9.94
9.93
9.86
9.75
9.63
9.55
9.44
9.36
9.27
9.17
9.1
9
8.92
8.85
9.17
9.17
9.17
9.17
9.16
9.15
9.15
9.15
9.15
9.14
9.15
9.15
9.14
9.14
9.13
9.13
9.13
9.12
9.12
9.11
9.11
9.1
9.1
9.09
9.08
9.08
9.08
9.07
9.07
9.06
9.06
8/17/2013 6:30
8/17/2013 6:40
8/17/2013 6:50
8/17/2013 7:00
8/17/2013 7:10
8/17/2013 7:20
8/17/2013 7:30
8/17/2013 7:40
8/17/2013 7:50
8/17/2013 8:00
8/17/2013 8:10
8/17/2013 8:20
8/17/2013 8:30
8/17/2013 8:40
8/17/2013 8:50
8/17/2013 9:00
8/17/2013 9:10
8/17/2013 9:20
8/17/2013 9:30
8/17/2013 9:40
8/17/2013 9:50
8/17/2013 10:00
8/17/2013 10:10
8/17/2013 10:20
8/17/2013 10:30
8/17/2013 10:40
8/17/2013 10:50
8/17/2013 11:00
8/17/2013 11:10
8/17/2013 11:20
8/17/2013 11:30
19.28
19.25
19.22
19.19
19.18
19.16
19.14
19.13
19.12
19.11
19.11
19.12
19.12
19.14
19.15
19.17
19.2
19.23
19.26
19.29
19.32
19.36
19.4
19.45
19.51
19.59
19.67
19.75
19.85
19.95
20.06
92
5.09
5.09
5.12
5.14
5.17
5.18
5.24
5.27
5.28
5.33
5.38
5.45
5.48
5.56
5.59
5.68
5.67
5.76
5.8
5.85
5.87
5.98
6.02
6.12
6.2
6.31
6.44
6.57
6.69
6.84
6.97
9.04
9.03
9.03
9.02
9.02
9.02
9.01
9
9
9
8.99
8.99
8.98
8.98
8.97
8.97
8.96
8.96
8.96
8.96
8.95
8.95
8.95
8.95
8.94
8.94
8.94
8.94
8.93
8.93
8.93
9/12/2013 19:30
9/12/2013 19:40
9/12/2013 19:50
9/12/2013 20:00
9/12/2013 20:10
9/12/2013 20:20
9/12/2013 20:30
9/12/2013 20:40
9/12/2013 20:50
9/12/2013 21:00
9/12/2013 21:10
9/12/2013 21:20
9/12/2013 21:30
9/12/2013 21:40
9/12/2013 21:50
9/12/2013 22:00
9/12/2013 22:10
9/12/2013 22:20
9/12/2013 22:30
9/12/2013 22:40
9/12/2013 22:50
9/12/2013 23:00
9/12/2013 23:10
9/12/2013 23:20
9/12/2013 23:30
9/12/2013 23:40
9/12/2013 23:50
9/13/2013 0:00
9/13/2013 0:10
9/13/2013 0:20
9/13/2013 0:30
23.63
23.58
23.53
23.48
23.43
23.39
23.33
23.29
23.25
23.21
23.18
23.14
23.1
23.07
23.04
23
22.95
22.9
22.84
22.8
22.75
22.7
22.65
22.61
22.56
22.52
22.47
22.43
22.39
22.34
22.3
4.57
4.57
4.51
4.5
4.48
4.43
4.42
4.41
4.38
4.37
4.35
4.33
4.29
4.3
4.25
4.24
4.22
4.19
4.18
4.15
4.15
4.11
4.09
4.07
4.06
4.02
4.01
3.97
3.96
3.95
3.96
9.16
9.15
9.14
9.14
9.13
9.12
9.11
9.1
9.1
9.09
9.08
9.07
9.06
9.05
9.05
9.04
9.03
9.03
9.02
9.01
9.01
9
8.99
8.99
8.98
8.98
8.97
8.97
8.97
8.96
8.95
7/29/2013 20:30
7/29/2013 20:40
7/29/2013 20:50
7/29/2013 21:00
7/29/2013 21:10
7/29/2013 21:20
7/29/2013 21:30
7/29/2013 21:40
7/29/2013 21:50
7/29/2013 22:00
7/29/2013 22:10
7/29/2013 22:20
7/29/2013 22:30
7/29/2013 22:40
7/29/2013 22:50
7/29/2013 23:00
7/29/2013 23:10
7/29/2013 23:20
7/29/2013 23:30
7/29/2013 23:40
7/29/2013 23:50
7/30/2013 0:00
7/30/2013 0:10
7/30/2013 0:20
7/30/2013 0:30
7/30/2013 0:40
7/30/2013 0:50
7/30/2013 1:00
7/30/2013 1:10
7/30/2013 1:20
7/30/2013 1:30
23.03
22.98
22.92
22.87
22.82
22.77
22.71
22.67
22.62
22.58
22.53
22.49
22.44
22.39
22.35
22.3
22.26
22.21
22.16
22.12
22.07
22.01
21.97
21.92
21.87
21.82
21.77
21.72
21.67
21.61
21.57
8.7
8.65
8.56
8.53
8.41
8.33
8.23
8.16
8.07
7.97
7.92
7.85
7.79
7.69
7.64
7.58
7.55
7.46
7.41
7.34
7.29
7.23
7.2
7.13
7.08
7.05
7.02
6.98
6.94
6.9
6.86
9.05
9.05
9.04
9.04
9.03
9.03
9.02
9.02
9.01
9.01
9
9
9
8.99
8.99
8.98
8.98
8.98
8.97
8.97
8.96
8.96
8.96
8.95
8.95
8.94
8.94
8.94
8.94
8.93
8.93
8/17/2013 11:40
8/17/2013 11:50
8/17/2013 12:00
8/17/2013 12:10
8/17/2013 12:20
8/17/2013 12:30
8/17/2013 12:40
8/17/2013 12:50
8/17/2013 13:00
8/17/2013 13:10
8/17/2013 13:20
8/17/2013 13:30
8/17/2013 13:40
8/17/2013 13:50
8/17/2013 14:00
8/17/2013 14:10
8/17/2013 14:20
8/17/2013 14:30
8/17/2013 14:40
8/17/2013 14:50
8/17/2013 15:00
8/17/2013 15:10
8/17/2013 15:20
8/17/2013 15:30
8/17/2013 15:40
8/17/2013 15:50
8/17/2013 16:00
8/17/2013 16:10
8/17/2013 16:20
8/17/2013 16:30
8/17/2013 16:40
20.18
20.29
20.37
20.47
20.59
20.72
20.82
20.87
20.93
21.03
21.13
21.27
21.34
21.54
21.6
21.82
22.03
22.19
22.15
22.23
22.22
22.26
22.38
22.5
22.5
22.46
22.46
22.47
22.47
22.56
22.54
93
7.1
7.24
7.34
7.4
7.49
7.55
7.6
7.65
7.66
7.75
7.62
7.71
7.86
7.99
8.02
8.06
8.24
8.33
8.41
8.47
8.36
8.47
8.56
8.68
8.82
8.89
8.88
8.91
8.9
8.86
8.76
8.93
8.93
8.92
8.92
8.92
8.93
8.92
8.93
8.93
8.93
8.93
8.94
8.94
8.94
8.94
8.95
8.95
8.95
8.96
8.96
8.96
8.97
8.97
8.97
8.98
8.99
9
9.01
9.02
9.03
9.04
9/13/2013 0:40
9/13/2013 0:50
9/13/2013 1:00
9/13/2013 1:10
9/13/2013 1:20
9/13/2013 1:30
9/13/2013 1:40
9/13/2013 1:50
9/13/2013 2:00
9/13/2013 2:10
9/13/2013 2:20
9/13/2013 2:30
9/13/2013 2:40
9/13/2013 2:50
9/13/2013 3:00
9/13/2013 3:10
9/13/2013 3:20
9/13/2013 3:30
9/13/2013 3:40
9/13/2013 3:50
9/13/2013 4:00
9/13/2013 4:10
9/13/2013 4:20
9/13/2013 4:30
9/13/2013 4:40
9/13/2013 4:50
9/13/2013 5:00
9/13/2013 5:10
9/13/2013 5:20
9/13/2013 5:30
9/13/2013 5:40
22.25
22.21
22.16
22.11
22.06
22.02
21.97
21.92
21.87
21.83
21.78
21.73
21.68
21.63
21.58
21.54
21.5
21.45
21.41
21.36
21.31
21.26
21.22
21.16
21.11
21.06
21.01
20.95
20.9
20.85
20.8
3.92
3.91
3.91
3.87
3.88
3.88
3.86
3.86
3.83
3.81
3.8
3.78
3.77
3.75
3.75
3.74
3.75
3.74
3.73
3.72
3.71
3.71
3.72
3.75
3.73
3.74
3.73
3.72
3.72
3.73
3.73
8.95
8.95
8.94
8.94
8.94
8.93
8.93
8.93
8.92
8.92
8.92
8.91
8.91
8.91
8.91
8.9
8.9
8.9
8.89
8.89
8.89
8.89
8.89
8.89
8.88
8.88
8.88
8.88
8.88
8.88
8.88
7/30/2013 1:40 21.51
7/30/2013 1:50 21.47
6.85 8.93
6.81 8.92
8/17/2013 16:50
8/17/2013 17:00
22.52
22.51
8.65 9.05
8.57 9.06
9/13/2013 5:50
9/13/2013 6:00
20.75 3.74
20.69 3.75
8.88
8.87
Appendix 1. (continued)
October
10/21/2013 21:00
10/21/2013 21:10
10/21/2013 21:20
10/21/2013 21:30
10/21/2013 21:40
10/21/2013 21:50
10/21/2013 22:00
10/21/2013 22:10
10/21/2013 22:20
10/21/2013 22:30
10/21/2013 22:40
10/21/2013 22:50
10/21/2013 23:00
10/21/2013 23:10
10/21/2013 23:20
10/21/2013 23:30
10/21/2013 23:40
10/21/2013 23:50
10/22/2013 0:00
10/22/2013 0:10
10/22/2013 0:20
10/22/2013 0:30
10/22/2013 0:40
10/22/2013 0:50
10/22/2013 1:00
10/22/2013 1:10
10/22/2013 1:20
Temp
13.15
13.14
13.12
13.11
13.09
13.07
13.04
13.02
12.99
12.96
12.92
12.90
12.87
12.84
12.80
12.77
12.74
12.70
12.66
12.63
12.59
12.55
12.51
12.47
12.43
12.38
12.34
DO
8.24
8.16
8.06
8.02
7.95
7.90
7.84
7.80
7.74
7.71
7.64
7.64
7.56
7.54
7.53
7.43
7.37
7.38
7.34
7.33
7.26
7.25
7.18
7.20
7.15
7.14
7.11
pH
9.08
9.11
9.12
9.12
9.12
9.11
9.11
9.10
9.10
9.09
9.09
9.08
9.08
9.07
9.06
9.06
9.05
9.05
9.05
9.04
9.04
9.03
9.03
9.03
9.02
9.02
9.01
November
11/8/2013 18:00
11/8/2013 18:10
11/8/2013 18:20
11/8/2013 18:30
11/8/2013 18:40
11/8/2013 18:50
11/8/2013 19:00
11/8/2013 19:10
11/8/2013 19:20
11/8/2013 19:30
11/8/2013 19:40
11/8/2013 19:50
11/8/2013 20:00
11/8/2013 20:10
11/8/2013 20:20
11/8/2013 20:30
11/8/2013 20:40
11/8/2013 20:50
11/8/2013 21:00
11/8/2013 21:10
11/8/2013 21:20
11/8/2013 21:30
11/8/2013 21:40
11/8/2013 21:50
11/8/2013 22:00
11/8/2013 22:10
11/8/2013 22:20
Temp
9.87
9.87
9.86
9.85
9.84
9.84
9.84
9.83
9.83
9.82
9.82
9.80
9.79
9.78
9.75
9.74
9.72
9.70
9.69
9.67
9.65
9.63
9.62
9.61
9.60
9.59
9.58
94
DO
11.47
11.45
11.35
11.29
11.24
11.13
11.03
11.00
10.90
10.86
10.74
10.68
10.59
10.54
10.46
10.37
10.28
10.23
10.18
10.12
10.06
10.01
9.94
9.87
9.82
9.77
9.79
pH
9.08
9.12
9.14
9.14
9.14
9.14
9.14
9.14
9.14
9.14
9.13
9.13
9.12
9.12
9.12
9.11
9.11
9.10
9.10
9.09
9.09
9.09
9.08
9.08
9.07
9.07
9.07
December
12/13/2013 20:00
12/13/2013 20:10
12/13/2013 20:20
12/13/2013 20:30
12/13/2013 20:40
12/13/2013 20:50
12/13/2013 21:00
12/13/2013 21:10
12/13/2013 21:20
12/13/2013 21:30
12/13/2013 21:40
12/13/2013 21:50
12/13/2013 22:00
12/13/2013 22:10
12/13/2013 22:20
12/13/2013 22:30
12/13/2013 22:40
12/13/2013 22:50
12/13/2013 23:00
12/13/2013 23:10
12/13/2013 23:20
12/13/2013 23:30
12/13/2013 23:40
12/13/2013 23:50
12/14/2013 0:00
12/14/2013 0:10
12/14/2013 0:20
Temp
2.65
2.61
2.56
2.52
2.47
2.45
2.43
2.42
2.39
2.36
2.33
2.28
2.22
2.18
2.13
2.09
2.04
1.99
1.95
1.90
1.85
1.79
1.76
1.71
1.68
1.69
1.69
DO
14.70
14.65
14.50
14.38
14.31
14.26
14.08
14.03
13.89
13.83
13.82
13.62
13.60
13.54
13.43
13.44
13.35
13.35
13.22
13.26
13.23
13.20
13.18
13.16
13.13
13.13
13.09
pH
9.70
9.69
9.69
9.68
9.67
9.66
9.65
9.64
9.63
9.63
9.62
9.61
9.60
9.60
9.59
9.58
9.58
9.57
9.57
9.57
9.56
9.56
9.55
9.55
9.54
9.54
9.53
10/22/2013 1:30
10/22/2013 1:40
10/22/2013 1:50
10/22/2013 2:00
10/22/2013 2:10
10/22/2013 2:20
10/22/2013 2:30
10/22/2013 2:40
10/22/2013 2:50
10/22/2013 3:00
10/22/2013 3:10
10/22/2013 3:20
10/22/2013 3:30
10/22/2013 3:40
10/22/2013 3:50
10/22/2013 4:00
10/22/2013 4:10
10/22/2013 4:20
10/22/2013 4:30
10/22/2013 4:40
10/22/2013 4:50
10/22/2013 5:00
10/22/2013 5:10
10/22/2013 5:20
10/22/2013 5:30
10/22/2013 5:40
10/22/2013 5:50
10/22/2013 6:00
10/22/2013 6:10
10/22/2013 6:20
10/22/2013 6:30
10/22/2013 6:40
10/22/2013 6:50
10/22/2013 7:00
12.30
12.26
12.21
12.17
12.12
12.07
12.03
11.98
11.93
11.89
11.85
11.80
11.76
11.71
11.67
11.62
11.58
11.54
11.50
11.45
11.40
11.36
11.32
11.28
11.23
11.19
11.15
11.12
11.08
11.04
11.00
10.96
10.93
10.89
7.12
7.05
7.07
7.04
7.01
7.00
6.99
6.97
6.98
6.96
6.93
6.94
6.95
6.96
6.92
6.93
6.92
6.92
6.90
6.91
6.89
6.89
6.92
6.93
6.92
6.90
6.91
6.94
6.89
6.90
6.91
6.91
6.94
6.92
9.01
9.01
9.00
9.00
9.00
8.99
8.99
8.99
8.98
8.98
8.98
8.97
8.97
8.97
8.97
8.96
8.96
8.96
8.96
8.96
8.96
8.96
8.96
8.96
8.96
8.95
8.95
8.95
8.95
8.95
8.95
8.95
8.95
8.95
11/8/2013 22:30
11/8/2013 22:40
11/8/2013 22:50
11/8/2013 23:00
11/8/2013 23:10
11/8/2013 23:20
11/8/2013 23:30
11/8/2013 23:40
11/8/2013 23:50
11/9/2013 0:00
11/9/2013 0:10
11/9/2013 0:20
11/9/2013 0:30
11/9/2013 0:40
11/9/2013 0:50
11/9/2013 1:00
11/9/2013 1:10
11/9/2013 1:20
11/9/2013 1:30
11/9/2013 1:40
11/9/2013 1:50
11/9/2013 2:00
11/9/2013 2:10
11/9/2013 2:20
11/9/2013 2:30
11/9/2013 2:40
11/9/2013 2:50
11/9/2013 3:00
11/9/2013 3:10
11/9/2013 3:20
11/9/2013 3:30
11/9/2013 3:40
11/9/2013 3:50
11/9/2013 4:00
9.56
9.54
9.52
9.51
9.49
9.47
9.45
9.44
9.41
9.39
9.37
9.34
9.32
9.30
9.27
9.26
9.23
9.22
9.20
9.18
9.17
9.15
9.13
9.12
9.10
9.08
9.07
9.06
9.04
9.03
9.02
9.01
8.99
8.98
95
9.70
9.68
9.64
9.55
9.57
9.53
9.46
9.47
9.41
9.37
9.39
9.36
9.32
9.34
9.31
9.29
9.26
9.24
9.21
9.22
9.19
9.17
9.20
9.17
9.15
9.18
9.10
9.11
9.16
9.14
9.13
9.10
9.10
9.13
9.06
9.06
9.06
9.05
9.05
9.05
9.05
9.05
9.04
9.04
9.04
9.04
9.04
9.04
9.04
9.03
9.04
9.03
9.03
9.03
9.03
9.03
9.02
9.03
9.03
9.02
9.02
9.03
9.02
9.02
9.02
9.02
9.03
9.02
12/14/2013 0:30
12/14/2013 0:40
12/14/2013 0:50
12/14/2013 1:00
12/14/2013 1:10
12/14/2013 1:20
12/14/2013 1:30
12/14/2013 1:40
12/14/2013 1:50
12/14/2013 2:00
12/14/2013 2:10
12/14/2013 2:20
12/14/2013 2:30
12/14/2013 2:40
12/14/2013 2:50
12/14/2013 3:00
12/14/2013 3:10
12/14/2013 3:20
12/14/2013 3:30
12/14/2013 3:40
12/14/2013 3:50
12/14/2013 4:00
12/14/2013 4:10
12/14/2013 4:20
12/14/2013 4:30
12/14/2013 4:40
12/14/2013 4:50
12/14/2013 5:00
12/14/2013 5:10
12/14/2013 5:20
12/14/2013 5:30
12/14/2013 5:40
12/14/2013 5:50
12/14/2013 6:00
1.70
1.70
1.70
1.69
1.68
1.67
1.67
1.66
1.65
1.65
1.65
1.64
1.65
1.64
1.63
1.62
1.62
1.62
1.61
1.59
1.55
1.55
1.56
1.58
1.59
1.59
1.58
1.58
1.59
1.59
1.60
1.61
1.62
1.63
13.04
12.99
13.00
13.01
13.01
13.03
13.02
13.02
12.97
12.98
12.99
12.96
12.95
12.95
12.94
12.93
12.90
12.91
12.91
12.87
12.82
12.80
12.79
12.76
12.76
12.74
12.74
12.73
12.67
12.67
12.68
12.64
12.62
12.61
9.53
9.53
9.52
9.52
9.52
9.52
9.51
9.51
9.51
9.51
9.51
9.51
9.51
9.51
9.50
9.50
9.50
9.50
9.50
9.49
9.49
9.49
9.49
9.48
9.48
9.48
9.48
9.48
9.47
9.47
9.47
9.47
9.46
9.46
10/22/2013 7:10
10/22/2013 7:20
10/22/2013 7:30
10/22/2013 7:40
10/22/2013 7:50
10/22/2013 8:00
10/22/2013 8:10
10/22/2013 8:20
10/22/2013 8:30
10/22/2013 8:40
10/22/2013 8:50
10/22/2013 9:00
10/22/2013 9:10
10/22/2013 9:20
10/22/2013 9:30
10/22/2013 9:40
10/22/2013 9:50
10/22/2013 10:00
10/22/2013 10:10
10/22/2013 10:20
10/22/2013 10:30
10/22/2013 10:40
10/22/2013 10:50
10/22/2013 11:00
10/22/2013 11:10
10/22/2013 11:20
10/22/2013 11:30
10/22/2013 11:40
10/22/2013 11:50
10/22/2013 12:00
10/22/2013 12:10
10/22/2013 12:20
10/22/2013 12:30
10/22/2013 12:40
10.85
10.81
10.77
10.74
10.71
10.69
10.66
10.63
10.61
10.60
10.59
10.58
10.59
10.59
10.59
10.59
10.58
10.59
10.59
10.60
10.62
10.63
10.65
10.68
10.71
10.76
10.80
10.84
10.88
10.94
11.01
11.05
11.09
11.14
6.93
6.95
6.94
6.97
7.01
7.04
7.08
7.12
7.19
7.24
7.36
7.41
7.48
7.55
7.56
7.67
7.74
7.80
7.81
7.92
7.95
8.07
8.17
8.24
8.37
8.45
8.59
8.68
8.80
8.87
8.99
9.04
9.14
9.23
8.95
8.95
8.95
8.95
8.95
8.95
8.95
8.96
8.96
8.97
8.98
8.98
8.98
8.99
8.99
9.00
9.00
9.01
9.01
9.01
9.02
9.03
9.04
9.05
9.05
9.06
9.07
9.08
9.08
9.09
9.10
9.11
9.12
9.13
11/9/2013 4:10
11/9/2013 4:20
11/9/2013 4:30
11/9/2013 4:40
11/9/2013 4:50
11/9/2013 5:00
11/9/2013 5:10
11/9/2013 5:20
11/9/2013 5:30
11/9/2013 5:40
11/9/2013 5:50
11/9/2013 6:00
11/9/2013 6:10
11/9/2013 6:20
11/9/2013 6:30
11/9/2013 6:40
11/9/2013 6:50
11/9/2013 7:00
11/9/2013 7:10
11/9/2013 7:20
11/9/2013 7:30
11/9/2013 7:40
11/9/2013 7:50
11/9/2013 8:00
11/9/2013 8:10
11/9/2013 8:20
11/9/2013 8:30
11/9/2013 8:40
11/9/2013 8:50
11/9/2013 9:00
11/9/2013 9:10
11/9/2013 9:20
11/9/2013 9:30
11/9/2013 9:40
8.97
8.95
8.93
8.92
8.89
8.87
8.85
8.82
8.80
8.79
8.78
8.76
8.74
8.72
8.71
8.69
8.67
8.65
8.64
8.61
8.60
8.58
8.57
8.57
8.56
8.55
8.54
8.54
8.55
8.56
8.56
8.58
8.61
8.62
96
9.09
9.11
9.06
9.12
9.10
9.08
9.05
9.08
9.07
9.05
9.06
9.09
9.09
9.04
9.01
9.05
9.03
9.02
9.02
9.05
9.08
9.05
9.09
9.09
9.10
9.17
9.22
9.23
9.28
9.34
9.38
9.42
9.45
9.53
9.02
9.02
9.02
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.03
9.04
9.04
9.04
9.05
9.05
9.05
9.05
9.06
12/14/2013 6:10
12/14/2013 6:20
12/14/2013 6:30
12/14/2013 6:40
12/14/2013 6:50
12/14/2013 7:00
12/14/2013 7:10
12/14/2013 7:20
12/14/2013 7:30
12/14/2013 7:40
12/14/2013 7:50
12/14/2013 8:00
12/14/2013 8:10
12/14/2013 8:20
12/14/2013 8:30
12/14/2013 8:40
12/14/2013 8:50
12/14/2013 9:00
12/14/2013 9:10
12/14/2013 9:20
12/14/2013 9:30
12/14/2013 9:40
12/14/2013 9:50
12/14/2013 10:00
12/14/2013 10:10
12/14/2013 10:20
12/14/2013 10:30
12/14/2013 10:40
12/14/2013 10:50
12/14/2013 11:00
12/14/2013 11:10
12/14/2013 11:20
12/14/2013 11:30
12/14/2013 11:40
1.65
1.67
1.69
1.71
1.72
1.74
1.74
1.75
1.73
1.72
1.73
1.73
1.74
1.75
1.76
1.77
1.77
1.78
1.79
1.81
1.83
1.85
1.86
1.86
1.86
1.85
1.85
1.84
1.82
1.83
1.83
1.83
1.84
1.85
12.64
12.60
12.57
12.58
12.54
12.55
12.51
12.48
12.49
12.45
12.44
12.47
12.48
12.47
12.46
12.46
12.56
12.51
12.58
12.62
12.67
12.73
12.82
12.83
12.90
13.00
13.07
13.09
13.23
13.29
13.40
13.52
13.75
13.83
9.46
9.46
9.46
9.45
9.45
9.45
9.45
9.45
9.45
9.45
9.45
9.45
9.45
9.44
9.45
9.44
9.44
9.45
9.45
9.45
9.45
9.45
9.45
9.45
9.46
9.46
9.46
9.47
9.47
9.48
9.49
9.49
9.50
9.51
10/22/2013 12:50
10/22/2013 13:00
10/22/2013 13:10
10/22/2013 13:20
10/22/2013 13:30
10/22/2013 13:40
10/22/2013 13:50
10/22/2013 14:00
10/22/2013 14:10
10/22/2013 14:20
10/22/2013 14:30
10/22/2013 14:40
10/22/2013 14:50
10/22/2013 15:00
10/22/2013 15:10
10/22/2013 15:20
10/22/2013 15:30
10/22/2013 15:40
10/22/2013 15:50
10/22/2013 16:00
10/22/2013 16:10
10/22/2013 16:20
10/22/2013 16:30
10/22/2013 16:40
10/22/2013 16:50
10/22/2013 17:00
10/22/2013 17:10
10/22/2013 17:20
10/22/2013 17:30
10/22/2013 17:40
10/22/2013 17:50
10/22/2013 18:00
10/22/2013 18:10
10/22/2013 18:20
11.20
11.26
11.32
11.35
11.38
11.42
11.45
11.48
11.49
11.50
11.52
11.52
11.53
11.52
11.52
11.51
11.52
11.52
11.53
11.53
11.53
11.53
11.53
11.54
11.54
11.54
11.54
11.53
11.53
11.52
11.52
11.51
11.50
11.49
9.36
9.42
9.57
9.71
9.80
9.94
10.01
10.09
10.21
10.27
10.38
10.39
10.45
10.46
10.48
10.52
10.55
10.59
10.60
10.58
10.61
10.60
10.57
10.58
10.62
10.64
10.55
10.52
10.45
10.42
10.38
10.31
10.19
10.11
9.14
9.15
9.16
9.17
9.17
9.18
9.19
9.20
9.21
9.22
9.23
9.23
9.24
9.25
9.25
9.26
9.26
9.27
9.28
9.28
9.29
9.30
9.30
9.30
9.31
9.31
9.31
9.32
9.32
9.32
9.32
9.32
9.32
9.32
11/9/2013 9:50
11/9/2013 10:00
11/9/2013 10:10
11/9/2013 10:20
11/9/2013 10:30
11/9/2013 10:40
11/9/2013 10:50
11/9/2013 11:00
11/9/2013 11:10
11/9/2013 11:20
11/9/2013 11:30
11/9/2013 11:40
11/9/2013 11:50
11/9/2013 12:00
11/9/2013 12:10
11/9/2013 12:20
11/9/2013 12:30
11/9/2013 12:40
11/9/2013 12:50
11/9/2013 13:00
11/9/2013 13:10
11/9/2013 13:20
11/9/2013 13:30
11/9/2013 13:40
11/9/2013 13:50
11/9/2013 14:00
11/9/2013 14:10
11/9/2013 14:20
11/9/2013 14:30
11/9/2013 14:40
11/9/2013 14:50
11/9/2013 15:00
11/9/2013 15:10
11/9/2013 15:20
8.65
8.67
8.71
8.74
8.77
8.81
8.85
8.89
8.94
8.99
9.05
9.11
9.17
9.23
9.30
9.36
9.44
9.51
9.58
9.65
9.72
9.78
9.85
9.92
9.99
10.07
10.14
10.20
10.25
10.29
10.33
10.36
10.40
10.44
97
9.59
9.70
9.69
9.81
9.89
9.94
9.98
10.09
10.19
10.26
10.33
10.44
10.52
10.55
10.67
10.76
10.81
10.94
10.99
11.06
11.11
11.18
11.31
11.35
11.37
11.40
11.56
11.65
11.67
11.70
11.68
11.66
11.70
11.73
9.06
9.07
9.07
9.08
9.08
9.08
9.09
9.09
9.10
9.11
9.11
9.12
9.13
9.13
9.14
9.14
9.14
9.15
9.16
9.16
9.17
9.18
9.18
9.19
9.20
9.20
9.21
9.21
9.22
9.22
9.22
9.23
9.23
9.23
12/14/2013 11:50
12/14/2013 12:00
12/14/2013 12:10
12/14/2013 12:20
12/14/2013 12:30
12/14/2013 12:40
12/14/2013 12:50
12/14/2013 13:00
12/14/2013 13:10
12/14/2013 13:20
12/14/2013 13:30
12/14/2013 13:40
12/14/2013 13:50
12/14/2013 14:00
12/14/2013 14:10
12/14/2013 14:20
12/14/2013 14:30
12/14/2013 14:40
12/14/2013 14:50
12/14/2013 15:00
12/14/2013 15:10
12/14/2013 15:20
12/14/2013 15:30
12/14/2013 15:40
12/14/2013 15:50
12/14/2013 16:00
12/14/2013 16:10
12/14/2013 16:20
12/14/2013 16:30
12/14/2013 16:40
12/14/2013 16:50
12/14/2013 17:00
12/14/2013 17:10
12/14/2013 17:20
1.87
1.87
1.86
1.83
1.82
1.83
1.81
1.78
1.78
1.80
1.82
1.84
1.86
1.86
1.87
1.87
1.87
1.87
1.87
1.86
1.86
1.85
1.85
1.85
1.85
1.85
1.87
1.89
1.89
1.91
1.92
1.93
1.95
1.97
13.93
13.94
13.96
14.16
14.27
14.47
14.51
14.60
14.70
14.80
14.90
15.03
15.15
15.27
15.37
15.43
15.54
15.59
15.66
15.64
15.71
15.72
15.75
15.80
15.92
15.91
15.79
15.81
15.78
15.75
15.66
15.60
15.59
15.49
9.52
9.53
9.53
9.54
9.55
9.56
9.57
9.58
9.59
9.59
9.61
9.61
9.62
9.63
9.64
9.65
9.66
9.66
9.67
9.68
9.68
9.69
9.69
9.70
9.71
9.71
9.71
9.72
9.72
9.72
9.72
9.72
9.71
9.71
10/22/2013 18:30
10/22/2013 18:40
10/22/2013 18:50
10/22/2013 19:00
10/22/2013 19:10
10/22/2013 19:20
10/22/2013 19:30
10/22/2013 19:40
10/22/2013 19:50
10/22/2013 20:00
10/22/2013 20:10
10/22/2013 20:20
10/22/2013 20:30
10/22/2013 20:40
10/22/2013 20:50
10/22/2013 21:00
10/22/2013 21:10
10/22/2013 21:20
10/22/2013 21:30
10/22/2013 21:40
10/22/2013 21:50
10/22/2013 22:00
10/22/2013 22:10
10/22/2013 22:20
10/22/2013 22:30
10/22/2013 22:40
10/22/2013 22:50
10/22/2013 23:00
10/22/2013 23:10
10/22/2013 23:20
10/22/2013 23:30
10/22/2013 23:40
10/22/2013 23:50
10/23/2013 0:00
11.48
11.46
11.43
11.40
11.38
11.36
11.34
11.33
11.32
11.30
11.28
11.27
11.26
11.25
11.24
11.24
11.23
11.21
11.21
11.20
11.19
11.18
11.17
11.17
11.16
11.15
11.15
11.14
11.13
11.12
11.11
11.10
11.09
11.09
10.05
9.97
9.90
9.82
9.73
9.69
9.61
9.54
9.44
9.39
9.32
9.21
9.14
9.06
8.98
8.92
8.88
8.77
8.73
8.67
8.62
8.52
8.51
8.39
8.36
8.30
8.29
8.21
8.17
8.13
8.08
8.02
8.00
7.94
9.31
9.31
9.31
9.30
9.30
9.29
9.28
9.28
9.27
9.26
9.25
9.24
9.23
9.22
9.21
9.20
9.19
9.18
9.17
9.16
9.15
9.15
9.14
9.13
9.12
9.11
9.11
9.10
9.09
9.09
9.08
9.07
9.07
9.06
11/9/2013 15:30
11/9/2013 15:40
11/9/2013 15:50
11/9/2013 16:00
11/9/2013 16:10
11/9/2013 16:20
11/9/2013 16:30
11/9/2013 16:40
11/9/2013 16:50
11/9/2013 17:00
11/9/2013 17:10
11/9/2013 17:20
11/9/2013 17:30
11/9/2013 17:40
11/9/2013 17:50
11/9/2013 18:00
11/9/2013 18:10
11/9/2013 18:20
11/9/2013 18:30
11/9/2013 18:40
11/9/2013 18:50
11/9/2013 19:00
11/9/2013 19:10
11/9/2013 19:20
11/9/2013 19:30
11/9/2013 19:40
11/9/2013 19:50
11/9/2013 20:00
11/9/2013 20:10
11/9/2013 20:20
11/9/2013 20:30
11/9/2013 20:40
11/9/2013 20:50
11/9/2013 21:00
10.47
10.49
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.50
10.49
10.48
10.47
10.46
10.45
10.43
10.42
10.41
10.40
10.40
10.39
10.38
10.37
10.36
10.35
10.34
10.33
98
11.83
11.76
11.78
11.79
11.71
11.68
11.68
11.61
11.58
11.58
11.55
11.49
11.46
11.46
11.41
11.34
11.29
11.21
11.14
11.06
11.04
10.90
10.81
10.68
10.64
10.54
10.45
10.34
10.30
10.19
10.12
10.05
9.96
9.96
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.24
9.23
9.23
9.23
9.22
9.22
9.21
9.21
9.20
9.20
9.19
9.18
9.18
9.17
9.17
9.16
9.15
9.15
9.14
12/14/2013 17:30
12/14/2013 17:40
12/14/2013 17:50
12/14/2013 18:00
12/14/2013 18:10
12/14/2013 18:20
12/14/2013 18:30
12/14/2013 18:40
12/14/2013 18:50
12/14/2013 19:00
12/14/2013 19:10
12/14/2013 19:20
12/14/2013 19:30
12/14/2013 19:40
12/14/2013 19:50
12/14/2013 20:00
12/14/2013 20:10
12/14/2013 20:20
12/14/2013 20:30
12/14/2013 20:40
12/14/2013 20:50
12/14/2013 21:00
12/14/2013 21:10
12/14/2013 21:20
12/14/2013 21:30
12/14/2013 21:40
12/14/2013 21:50
12/14/2013 22:00
12/14/2013 22:10
12/14/2013 22:20
12/14/2013 22:30
12/14/2013 22:40
12/14/2013 22:50
12/14/2013 23:00
1.98
2.01
2.03
2.05
2.07
2.08
2.10
2.12
2.13
2.14
2.15
2.17
2.18
2.20
2.21
2.22
2.24
2.25
2.26
2.26
2.27
2.26
2.27
2.27
2.27
2.27
2.27
2.27
2.27
2.27
2.27
2.27
2.27
2.28
15.34
15.35
15.22
15.11
14.98
14.91
14.84
14.69
14.62
14.49
14.41
14.29
14.16
14.05
13.95
13.88
13.76
13.71
13.63
13.53
13.44
13.38
13.32
13.24
13.12
13.04
12.98
12.99
12.95
12.88
12.84
12.76
12.73
12.68
9.71
9.70
9.70
9.69
9.69
9.68
9.67
9.66
9.65
9.65
9.64
9.63
9.62
9.61
9.60
9.59
9.59
9.58
9.57
9.56
9.55
9.54
9.54
9.53
9.52
9.52
9.51
9.50
9.50
9.49
9.48
9.48
9.47
9.47
10/23/2013 0:10
10/23/2013 0:20
10/23/2013 0:30
10/23/2013 0:40
10/23/2013 0:50
10/23/2013 1:00
10/23/2013 1:10
10/23/2013 1:20
10/23/2013 1:30
10/23/2013 1:40
10/23/2013 1:50
10/23/2013 2:00
10/23/2013 2:10
10/23/2013 2:20
10/23/2013 2:30
10/23/2013 2:40
10/23/2013 2:50
10/23/2013 3:00
10/23/2013 3:10
10/23/2013 3:20
10/23/2013 3:30
10/23/2013 3:40
10/23/2013 3:50
10/23/2013 4:00
10/23/2013 4:10
10/23/2013 4:20
10/23/2013 4:30
10/23/2013 4:40
10/23/2013 4:50
10/23/2013 5:00
10/23/2013 5:10
10/23/2013 5:20
10/23/2013 5:30
10/23/2013 5:40
11.07
11.05
11.05
11.04
11.02
10.98
10.97
10.94
10.89
10.83
10.76
10.70
10.64
10.59
10.54
10.46
10.40
10.35
10.30
10.27
10.24
10.24
10.24
10.23
10.22
10.19
10.17
10.14
10.09
10.05
10.01
9.97
9.93
9.90
7.92
7.85
7.80
7.76
7.74
7.66
7.67
7.64
7.59
7.57
7.54
7.48
7.48
7.45
7.41
7.43
7.38
7.35
7.33
7.33
7.32
7.30
7.27
7.25
7.24
7.23
7.25
7.21
7.22
7.17
7.20
7.22
7.20
7.20
9.06
9.05
9.04
9.04
9.04
9.03
9.03
9.02
9.02
9.01
9.01
9.01
9.01
9.00
9.00
9.00
9.00
8.99
8.99
8.99
8.99
8.98
8.98
8.98
8.98
8.97
8.97
8.98
8.97
8.97
8.97
8.97
8.96
8.96
11/9/2013 21:10
11/9/2013 21:20
11/9/2013 21:30
11/9/2013 21:40
11/9/2013 21:50
11/9/2013 22:00
11/9/2013 22:10
11/9/2013 22:20
11/9/2013 22:30
11/9/2013 22:40
11/9/2013 22:50
11/9/2013 23:00
11/9/2013 23:10
11/9/2013 23:20
11/9/2013 23:30
11/9/2013 23:40
11/9/2013 23:50
11/10/2013 0:00
11/10/2013 0:10
11/10/2013 0:20
11/10/2013 0:30
11/10/2013 0:40
11/10/2013 0:50
11/10/2013 1:00
11/10/2013 1:10
11/10/2013 1:20
11/10/2013 1:30
11/10/2013 1:40
11/10/2013 1:50
11/10/2013 2:00
11/10/2013 2:10
11/10/2013 2:20
11/10/2013 2:30
11/10/2013 2:40
10.32
10.31
10.30
10.29
10.28
10.27
10.26
10.24
10.23
10.21
10.20
10.18
10.16
10.14
10.13
10.10
10.09
10.08
10.06
10.04
10.03
10.01
10.00
9.98
9.95
9.94
9.91
9.89
9.87
9.84
9.82
9.79
9.76
9.74
99
9.86
9.83
9.72
9.68
9.63
9.54
9.49
9.48
9.39
9.35
9.38
9.28
9.25
9.22
9.18
9.14
9.09
9.07
9.06
9.01
8.96
8.98
8.92
8.90
8.89
8.88
8.86
8.90
8.83
8.83
8.77
8.85
8.79
8.81
9.14
9.13
9.13
9.12
9.12
9.11
9.11
9.11
9.10
9.10
9.09
9.09
9.09
9.08
9.08
9.08
9.07
9.07
9.07
9.06
9.06
9.06
9.05
9.05
9.05
9.05
9.05
9.04
9.04
9.04
9.04
9.04
9.04
9.04
12/14/2013 23:10
12/14/2013 23:20
12/14/2013 23:30
12/14/2013 23:40
12/14/2013 23:50
12/15/2013 0:00
12/15/2013 0:10
12/15/2013 0:20
12/15/2013 0:30
12/15/2013 0:40
12/15/2013 0:50
12/15/2013 1:00
12/15/2013 1:10
12/15/2013 1:20
12/15/2013 1:30
12/15/2013 1:40
12/15/2013 1:50
12/15/2013 2:00
12/15/2013 2:10
12/15/2013 2:20
12/15/2013 2:30
12/15/2013 2:40
12/15/2013 2:50
12/15/2013 3:00
12/15/2013 3:10
12/15/2013 3:20
12/15/2013 3:30
12/15/2013 3:40
12/15/2013 3:50
12/15/2013 4:00
12/15/2013 4:10
12/15/2013 4:20
12/15/2013 4:30
12/15/2013 4:40
2.28
2.28
2.27
2.26
2.25
2.23
2.22
2.21
2.21
2.20
2.20
2.20
2.20
2.21
2.21
2.22
2.22
2.23
2.23
2.23
2.22
2.21
2.21
2.19
2.18
2.17
2.16
2.15
2.13
2.12
2.10
2.09
2.07
2.05
12.71
12.66
12.66
12.57
12.56
12.53
12.58
12.53
12.48
12.47
12.46
12.43
12.45
12.44
12.41
12.37
12.37
12.37
12.37
12.32
12.35
12.32
12.32
12.29
12.33
12.26
12.26
12.32
12.21
12.33
12.29
12.24
12.30
12.30
9.46
9.46
9.46
9.45
9.45
9.45
9.45
9.44
9.44
9.44
9.44
9.43
9.43
9.43
9.42
9.42
9.42
9.42
9.41
9.41
9.41
9.41
9.41
9.41
9.41
9.41
9.41
9.41
9.40
9.40
9.40
9.40
9.40
9.40
10/23/2013 5:50
10/23/2013 6:00
9.86
9.84
7.19
7.20
8.97
8.96
11/10/2013 2:50
11/10/2013 3:00
9.71
9.68
100
8.81
8.75
9.04
9.04
12/15/2013 4:50
12/15/2013 5:00
2.04
2.02
12.28
12.28
9.40
9.40
Appendix 2.
Algae Physiochemical Measurements
January
1/25/2013 10:00
1/25/2013 10:10
1/25/2013 10:20
1/25/2013 10:30
1/25/2013 10:40
1/25/2013 10:50
1/25/2013 11:00
1/25/2013 11:10
1/25/2013 11:20
1/25/2013 11:30
1/25/2013 11:40
1/25/2013 11:50
1/25/2013 12:00
1/25/2013 12:10
1/25/2013 12:20
1/25/2013 12:30
1/25/2013 12:40
1/25/2013 12:50
1/25/2013 13:00
1/25/2013 13:10
1/25/2013 13:20
1/25/2013 13:30
1/25/2013 13:40
1/25/2013 13:50
1/25/2013 14:00
1/25/2013 14:10
1/25/2013 14:20
1/25/2013 14:30
1/25/2013 14:40
1/25/2013 14:50
1/25/2013 15:00
DO
5.26
5.42
5.66
5.71
5.74
5.74
5.81
5.78
5.80
5.79
5.80
5.80
5.81
5.83
5.84
5.84
5.85
5.86
5.87
5.89
5.91
5.93
5.95
5.96
5.97
5.98
6.00
6.01
6.02
6.03
6.04
DO
5.86
5.60
5.68
5.74
5.76
5.79
5.81
5.84
5.87
5.90
5.92
5.95
5.98
6.00
6.03
6.12
6.09
6.15
6.16
6.21
6.23
6.26
6.30
6.33
6.31
6.33
6.35
6.34
6.35
6.38
6.36
pH
8.66
8.74
8.69
8.67
8.67
8.66
8.65
8.65
8.64
8.63
8.63
8.62
8.65
8.64
8.65
8.65
8.64
8.64
8.64
8.63
8.62
8.62
8.62
8.61
8.61
8.61
8.61
8.61
8.62
8.61
8.61
February
2/7/2013 21:09
2/7/2013 21:19
2/7/2013 21:29
2/7/2013 21:39
2/7/2013 21:49
2/7/2013 21:59
2/7/2013 22:09
2/7/2013 22:19
2/7/2013 22:29
2/7/2013 22:39
2/7/2013 22:49
2/7/2013 22:59
2/7/2013 23:09
2/7/2013 23:19
2/7/2013 23:29
2/7/2013 23:39
2/7/2013 23:49
2/7/2013 23:59
2/8/2013 0:09
2/8/2013 0:19
2/8/2013 0:29
2/8/2013 0:39
2/8/2013 0:49
2/8/2013 0:59
2/8/2013 1:09
2/8/2013 1:19
2/8/2013 1:29
2/8/2013 1:39
2/8/2013 1:49
2/8/2013 1:59
2/8/2013 2:09
DO
6.62
6.62
6.64
6.63
6.61
6.62
6.63
6.61
6.57
6.53
6.48
6.45
6.42
6.40
6.37
6.39
6.37
6.34
6.31
6.25
6.25
6.25
6.25
6.24
6.23
6.24
6.37
6.34
6.32
6.31
6.29
DO
6.32
6.26
6.21
6.18
6.17
6.15
6.14
6.12
6.11
6.10
6.09
6.08
6.07
6.06
6.05
6.05
6.04
6.03
6.03
6.03
6.02
6.01
6.01
6.00
6.00
5.99
5.98
5.98
5.98
5.98
5.98
101
pH
8.79
8.76
8.67
8.76
8.77
8.73
8.75
8.72
8.71
8.73
8.73
8.75
8.69
8.66
8.64
8.64
8.62
8.67
8.66
8.68
8.71
8.68
8.67
8.70
8.67
8.67
8.68
8.68
8.66
8.65
8.65
March
2/28/2013 20:51
2/28/2013 21:01
2/28/2013 21:11
2/28/2013 21:21
2/28/2013 21:31
2/28/2013 21:41
2/28/2013 21:51
2/28/2013 22:01
2/28/2013 22:11
2/28/2013 22:21
2/28/2013 22:31
2/28/2013 22:41
2/28/2013 22:51
2/28/2013 23:01
2/28/2013 23:11
2/28/2013 23:21
2/28/2013 23:31
2/28/2013 23:41
2/28/2013 23:51
3/1/2013 0:01
3/1/2013 0:11
3/1/2013 0:21
3/1/2013 0:31
3/1/2013 0:41
3/1/2013 0:51
3/1/2013 1:01
3/1/2013 1:11
3/1/2013 1:21
3/1/2013 1:31
3/1/2013 1:41
3/1/2013 1:51
DO
5.20
5.25
5.31
5.35
5.37
5.40
5.47
5.48
5.49
5.50
5.50
5.51
5.52
5.50
5.51
5.52
5.51
5.51
5.52
5.53
5.54
5.55
5.54
5.54
5.55
5.55
5.54
5.53
5.51
5.49
5.48
DO
4.78
4.81
4.85
4.87
4.89
4.90
4.91
4.92
4.92
4.92
4.91
4.92
4.91
4.92
4.91
4.91
4.90
4.91
4.88
4.87
4.86
4.86
4.86
4.86
4.85
4.85
4.84
4.82
4.81
4.81
4.80
pH
8.08
8.10
8.07
8.21
8.09
8.09
8.09
8.09
8.12
8.09
8.10
8.09
8.08
8.10
8.09
8.11
8.08
8.10
8.06
8.13
8.10
8.09
8.07
8.08
8.07
8.07
8.05
8.08
8.04
8.03
8.08
April
4/15/2013 0:04
4/15/2013 0:14
4/15/2013 0:24
4/15/2013 0:34
4/15/2013 0:44
4/15/2013 0:54
4/15/2013 1:04
4/15/2013 1:14
4/15/2013 1:24
4/15/2013 1:34
4/15/2013 1:44
4/15/2013 1:54
4/15/2013 2:04
4/15/2013 2:14
4/15/2013 2:24
4/15/2013 2:34
4/15/2013 2:44
4/15/2013 2:54
4/15/2013 3:04
4/15/2013 3:14
4/15/2013 3:24
4/15/2013 3:34
4/15/2013 3:44
4/15/2013 3:54
4/15/2013 4:04
4/15/2013 4:14
4/15/2013 4:24
4/15/2013 4:34
4/15/2013 4:44
4/15/2013 4:54
4/15/2013 5:04
DO
6.73
6.75
6.73
6.73
6.74
6.75
6.67
6.66
6.66
6.67
6.64
6.65
6.66
6.65
6.64
6.63
6.63
6.63
6.61
6.54
6.55
6.59
6.56
6.57
6.57
6.57
6.57
6.57
6.54
6.56
6.56
1/25/2013 15:10
1/25/2013 15:20
1/25/2013 15:30
1/25/2013 15:40
1/25/2013 15:50
1/25/2013 16:00
1/25/2013 16:10
1/25/2013 16:20
1/25/2013 16:30
1/25/2013 16:40
1/25/2013 16:50
1/25/2013 17:00
1/25/2013 17:10
1/25/2013 17:20
1/25/2013 17:30
1/25/2013 17:40
1/25/2013 17:50
1/25/2013 18:00
1/25/2013 18:10
1/25/2013 18:20
1/25/2013 18:30
1/25/2013 18:40
1/25/2013 18:50
1/25/2013 19:00
1/25/2013 19:10
1/25/2013 19:20
1/25/2013 19:30
1/25/2013 19:40
1/25/2013 19:50
1/25/2013 20:00
1/25/2013 20:10
1/25/2013 20:20
1/25/2013 20:30
1/25/2013 20:40
6.04
6.05
6.04
6.04
6.04
6.04
6.02
6.01
6.00
5.99
5.97
5.96
5.94
5.92
5.91
5.89
5.87
5.85
5.83
5.82
5.79
5.78
5.76
5.75
5.74
5.72
5.70
5.69
5.67
5.66
5.65
5.63
5.61
5.62
6.39
6.33
6.33
6.33
6.33
6.32
6.31
6.31
6.30
6.27
6.26
6.24
6.23
6.20
6.18
6.16
6.15
6.13
6.11
6.09
6.07
6.04
6.03
6.00
5.99
5.98
5.96
5.94
5.93
5.92
5.90
5.88
5.86
5.85
8.61
8.59
8.60
8.61
8.61
8.61
8.61
8.61
8.62
8.62
8.62
8.62
8.61
8.61
8.62
8.62
8.62
8.63
8.62
8.62
8.63
8.63
8.63
8.63
8.62
8.62
8.62
8.62
8.62
8.62
8.62
8.62
8.63
8.63
2/8/2013 2:19
2/8/2013 2:29
2/8/2013 2:39
2/8/2013 2:49
2/8/2013 2:59
2/8/2013 3:09
2/8/2013 3:19
2/8/2013 3:29
2/8/2013 3:39
2/8/2013 3:49
2/8/2013 3:59
2/8/2013 4:09
2/8/2013 4:19
2/8/2013 4:29
2/8/2013 4:39
2/8/2013 4:49
2/8/2013 4:59
2/8/2013 5:09
2/8/2013 5:19
2/8/2013 5:29
2/8/2013 5:39
2/8/2013 5:49
2/8/2013 5:59
2/8/2013 6:09
2/8/2013 6:19
2/8/2013 6:29
2/8/2013 6:39
2/8/2013 6:49
2/8/2013 6:59
2/8/2013 7:09
2/8/2013 7:19
2/8/2013 7:29
2/8/2013 7:39
2/8/2013 7:49
6.27
6.27
6.26
6.34
6.32
6.27
6.25
6.23
6.20
6.22
6.19
6.21
6.22
6.21
6.22
6.21
6.18
6.20
6.15
6.15
6.14
6.12
6.09
6.04
6.03
6.03
5.99
5.93
5.90
5.86
5.83
5.82
5.85
5.81
5.96
5.96
5.95
5.95
5.95
5.94
5.94
5.93
5.92
5.93
5.92
5.92
5.91
5.91
5.90
5.89
5.89
5.88
5.88
5.87
5.86
5.85
5.81
5.79
5.77
5.76
5.74
5.73
5.71
5.70
5.70
5.69
5.70
5.70
102
8.66
8.66
8.65
8.64
8.63
8.58
8.55
8.44
8.55
8.48
8.42
8.53
8.50
8.48
8.45
8.44
8.53
8.48
8.42
8.50
8.47
8.41
8.43
8.42
8.44
8.42
8.42
8.37
8.38
8.42
8.38
8.42
8.40
8.43
3/1/2013 2:01
3/1/2013 2:11
3/1/2013 2:21
3/1/2013 2:31
3/1/2013 2:41
3/1/2013 2:51
3/1/2013 3:01
3/1/2013 3:11
3/1/2013 3:21
3/1/2013 3:31
3/1/2013 3:41
3/1/2013 3:51
3/1/2013 4:01
3/1/2013 4:11
3/1/2013 4:21
3/1/2013 4:31
3/1/2013 4:41
3/1/2013 4:51
3/1/2013 5:01
3/1/2013 5:11
3/1/2013 5:21
3/1/2013 5:31
3/1/2013 5:41
3/1/2013 5:51
3/1/2013 6:01
3/1/2013 6:11
3/1/2013 6:21
3/1/2013 6:31
3/1/2013 6:41
3/1/2013 6:51
3/1/2013 7:01
3/1/2013 7:11
3/1/2013 7:21
3/1/2013 7:31
5.52
5.52
5.51
5.52
5.50
5.49
5.49
5.49
5.48
5.48
5.47
5.48
5.48
5.48
5.48
5.47
5.47
5.47
5.47
5.46
5.46
5.46
5.46
5.45
5.41
5.43
5.42
5.39
5.41
5.35
5.37
5.36
5.35
5.34
4.80
4.81
4.81
4.81
4.81
4.82
4.81
4.81
4.78
4.80
4.80
4.79
4.83
4.74
4.85
4.74
4.78
4.73
4.74
4.80
4.76
4.75
4.82
4.73
4.66
4.81
4.74
4.74
4.73
4.70
4.73
4.73
4.73
4.67
8.09
8.14
8.47
8.37
8.32
8.27
8.22
8.20
8.16
8.11
8.09
8.06
8.03
8.03
8.00
7.98
7.96
7.94
7.92
7.91
7.91
7.90
7.91
7.93
7.92
7.94
7.94
7.99
7.97
7.98
8.01
8.01
8.05
8.02
4/15/2013 5:14
4/15/2013 5:24
4/15/2013 5:34
4/15/2013 5:44
4/15/2013 5:54
4/15/2013 6:04
4/15/2013 6:14
4/15/2013 6:24
4/15/2013 6:34
4/15/2013 6:44
4/15/2013 6:54
4/15/2013 7:04
4/15/2013 7:14
4/15/2013 7:24
4/15/2013 7:34
4/15/2013 7:44
4/15/2013 7:54
4/15/2013 8:04
4/15/2013 8:14
4/15/2013 8:24
4/15/2013 8:34
4/15/2013 8:44
4/15/2013 8:54
4/15/2013 9:04
4/15/2013 9:14
4/15/2013 9:24
4/15/2013 9:34
4/15/2013 9:44
4/15/2013 9:54
4/15/2013 10:04
4/15/2013 10:14
4/15/2013 10:24
4/15/2013 10:34
4/15/2013 10:44
6.55
6.53
6.55
6.54
6.54
6.52
6.55
6.55
6.52
6.51
6.56
6.56
6.57
6.57
6.59
6.64
6.67
6.69
6.73
6.76
6.80
6.83
6.88
6.94
6.97
7.01
7.06
7.11
7.18
7.25
7.32
7.39
7.52
7.62
1/25/2013 20:50
1/25/2013 21:00
1/25/2013 21:10
1/25/2013 21:20
1/25/2013 21:30
1/25/2013 21:40
1/25/2013 21:50
1/25/2013 22:00
1/25/2013 22:10
1/25/2013 22:20
1/25/2013 22:30
1/25/2013 22:40
1/25/2013 22:50
1/25/2013 23:00
1/25/2013 23:10
1/25/2013 23:20
1/25/2013 23:30
1/25/2013 23:40
1/25/2013 23:50
1/26/2013 0:00
1/26/2013 0:10
1/26/2013 0:20
1/26/2013 0:30
1/26/2013 0:40
1/26/2013 0:50
1/26/2013 1:00
1/26/2013 1:10
1/26/2013 1:20
1/26/2013 1:30
1/26/2013 1:40
1/26/2013 1:50
1/26/2013 2:00
1/26/2013 2:10
1/26/2013 2:20
5.60
5.60
5.59
5.60
5.60
5.59
5.58
5.59
5.58
5.59
5.59
5.58
5.58
5.57
5.57
5.57
5.56
5.56
5.55
5.55
5.55
5.53
5.56
5.53
5.53
5.51
5.52
5.51
5.50
5.51
5.50
5.49
5.49
5.48
5.84
5.83
5.82
5.82
5.82
5.81
5.80
5.80
5.79
5.79
5.78
5.78
5.77
5.77
5.77
5.76
5.76
5.76
5.75
5.75
5.74
5.73
5.73
5.72
5.72
5.71
5.70
5.69
5.69
5.69
5.69
5.69
5.69
5.68
8.63
8.62
8.62
8.61
8.61
8.61
8.60
8.61
8.60
8.60
8.60
8.60
8.60
8.60
8.60
8.60
8.59
8.60
8.60
8.60
8.60
8.60
8.59
8.59
8.59
8.59
8.59
8.59
8.59
8.60
8.59
8.60
8.60
8.60
2/8/2013 7:59
2/8/2013 8:09
2/8/2013 8:19
2/8/2013 8:29
2/8/2013 8:39
2/8/2013 8:49
2/8/2013 8:59
2/8/2013 9:09
2/8/2013 9:19
2/8/2013 9:29
2/8/2013 9:39
2/8/2013 9:49
2/8/2013 9:59
2/8/2013 10:09
2/8/2013 10:19
2/8/2013 10:29
2/8/2013 10:39
2/8/2013 10:49
2/8/2013 10:59
2/8/2013 11:09
2/8/2013 11:19
2/8/2013 11:29
2/8/2013 11:39
2/8/2013 11:49
2/8/2013 11:59
2/8/2013 12:09
2/8/2013 12:19
2/8/2013 12:29
2/8/2013 12:39
2/8/2013 12:49
2/8/2013 12:59
2/8/2013 13:09
2/8/2013 13:19
2/8/2013 13:29
5.81
5.81
5.81
5.78
5.76
5.90
5.94
5.94
5.84
5.79
5.80
5.74
5.78
5.84
5.76
5.86
5.87
5.89
5.91
5.85
5.99
6.03
6.03
6.02
5.97
5.94
6.11
6.13
6.13
6.15
6.15
6.16
6.21
6.37
5.69
5.70
5.69
5.70
5.70
5.71
5.71
5.72
5.72
5.72
5.72
5.72
5.73
5.75
5.76
5.77
5.78
5.79
5.82
5.84
5.86
5.88
5.90
5.92
5.93
5.94
5.96
5.97
5.99
6.00
6.01
6.02
6.04
6.06
103
8.43
8.40
8.41
8.43
8.42
8.39
8.38
8.44
8.39
8.39
8.38
8.37
8.38
8.38
8.38
8.43
8.39
8.38
8.39
8.37
8.40
8.38
8.39
8.38
8.51
8.44
8.49
8.48
8.52
8.63
8.56
8.56
8.50
8.58
3/1/2013 7:41 5.37 4.78
3/1/2013 7:51 5.36 4.83
3/1/2013 8:01 5.41 4.80
3/1/2013 8:11 5.40 4.82
3/1/2013 8:21 5.40 4.85
3/1/2013 8:31 5.46 4.86
3/1/2013 8:41 5.52 4.92
3/1/2013 8:51 5.55 4.83
3/1/2013 9:01 5.54 4.81
3/1/2013 9:11 5.51 4.83
3/1/2013 9:21 5.60 4.92
3/1/2013 9:31 6.05 4.71
3/1/2013 9:41 8.43 5.14
3/1/2013 9:51 8.23 5.05
3/1/2013 10:01 8.91 5.12
3/1/2013 10:11 8.35 5.13
3/1/2013 10:21 9.01 5.17
3/1/2013 10:31 8.28 5.11
3/1/2013 10:41 7.87 5.07
3/1/2013 10:51 7.99 5.10
3/1/2013 11:01 8.63 5.14
3/1/2013 11:11 8.61 5.15
3/1/2013 11:21 9.52 5.20
3/1/2013 11:31 8.60 5.11
3/1/2013 11:41 8.47 5.11
3/1/2013 11:51 9.02 5.13
3/1/2013 12:01 8.79 5.15
3/1/2013 12:11 9.23 5.13
3/1/2013 12:21 9.38 5.16
3/1/2013 12:31 9.12 5.14
3/1/2013 12:41 8.87 5.22
3/1/2013 12:51 10.14 5.16
3/1/2013 13:01 9.91 5.20
3/1/2013 13:11 9.42 5.33
8.04
8.05
8.07
8.06
8.08
8.06
8.05
8.06
8.07
8.05
8.06
8.06
8.04
8.02
8.01
8.02
7.98
8.00
7.96
4/15/2013 10:54
4/15/2013 11:04
4/15/2013 11:14
4/15/2013 11:24
4/15/2013 11:34
4/15/2013 11:44
4/15/2013 11:54
4/15/2013 12:04
4/15/2013 12:14
4/15/2013 12:24
4/15/2013 12:34
4/15/2013 12:44
4/15/2013 12:54
4/15/2013 13:04
4/15/2013 13:14
4/15/2013 13:24
4/15/2013 13:34
4/15/2013 13:44
4/15/2013 13:54
4/15/2013 14:04
4/15/2013 14:14
4/15/2013 14:24
4/15/2013 14:34
4/15/2013 14:44
4/15/2013 14:54
4/15/2013 15:04
4/15/2013 15:14
4/15/2013 15:24
4/15/2013 15:34
4/15/2013 15:44
4/15/2013 15:54
4/15/2013 16:04
4/15/2013 16:14
4/15/2013 16:24
7.70
7.79
7.90
8.07
8.26
8.39
8.41
8.45
8.49
8.54
8.53
8.51
8.61
8.69
8.67
8.66
8.71
8.80
8.79
8.80
8.85
8.87
8.88
8.89
8.97
9.11
9.17
9.10
9.14
9.24
9.27
9.21
9.24
9.26
1/26/2013 2:30
1/26/2013 2:40
1/26/2013 2:50
1/26/2013 3:00
1/26/2013 3:10
1/26/2013 3:20
1/26/2013 3:30
1/26/2013 3:40
1/26/2013 3:50
1/26/2013 4:00
1/26/2013 4:10
1/26/2013 4:20
1/26/2013 4:30
1/26/2013 4:40
1/26/2013 4:50
1/26/2013 5:00
1/26/2013 5:10
1/26/2013 5:20
1/26/2013 5:30
1/26/2013 5:40
1/26/2013 5:50
1/26/2013 6:00
1/26/2013 6:10
1/26/2013 6:20
1/26/2013 6:38
1/26/2013 6:48
1/26/2013 6:58
1/26/2013 7:08
1/26/2013 7:18
1/26/2013 7:28
1/26/2013 7:38
1/26/2013 7:48
1/26/2013 7:58
1/26/2013 8:08
5.48
5.48
5.48
5.46
5.47
5.46
5.46
5.46
5.45
5.45
5.45
5.44
5.44
5.42
5.42
5.41
5.40
5.40
5.39
5.39
5.40
5.39
5.39
5.39
5.38
5.40
5.42
5.42
5.42
5.43
5.43
5.43
5.43
5.44
5.68
5.67
5.67
5.66
5.65
5.65
5.65
5.64
5.64
5.63
5.63
5.63
5.62
5.61
5.61
5.60
5.60
5.59
5.59
5.59
5.59
5.59
5.60
5.59
5.60
5.60
5.60
5.60
5.60
5.60
5.60
5.61
5.61
5.61
8.60
8.60
8.60
8.60
8.60
8.60
8.59
8.60
8.60
8.60
8.60
8.60
8.60
8.60
8.60
8.61
8.60
8.61
8.61
8.60
8.60
8.60
8.59
8.59
8.58
8.59
8.59
8.58
8.59
8.59
8.58
8.59
8.60
8.59
2/8/2013 13:39
2/8/2013 13:49
2/8/2013 13:59
2/8/2013 14:09
2/8/2013 14:19
2/8/2013 14:29
2/8/2013 14:39
2/8/2013 14:49
2/8/2013 14:59
2/8/2013 15:09
2/8/2013 15:19
2/8/2013 15:29
2/8/2013 15:39
2/8/2013 15:49
2/8/2013 15:59
2/8/2013 16:09
2/8/2013 16:19
2/8/2013 16:29
2/8/2013 16:39
2/8/2013 16:49
2/8/2013 16:59
2/8/2013 17:09
2/8/2013 17:19
2/8/2013 17:29
2/8/2013 17:39
2/8/2013 17:49
2/8/2013 17:59
2/8/2013 18:09
2/8/2013 18:19
2/8/2013 18:29
2/8/2013 18:39
2/8/2013 18:49
2/8/2013 18:59
2/8/2013 19:09
6.41
6.44
6.46
6.47
6.47
6.48
6.48
6.48
6.48
6.48
6.48
6.49
6.48
6.46
6.45
6.43
6.41
6.40
6.37
6.35
6.34
6.32
6.30
6.28
6.26
6.26
6.24
6.22
6.20
6.17
6.14
6.57
6.54
6.51
6.09
6.10
6.12
6.14
6.15
6.16
6.17
6.17
6.19
6.20
6.21
6.21
6.20
6.20
6.20
6.19
6.18
6.18
6.17
6.14
6.13
6.13
6.11
6.09
6.06
6.04
6.00
5.99
5.97
5.95
5.93
5.91
5.89
5.87
104
8.52
8.55
8.52
8.55
8.51
8.51
8.53
8.54
8.48
8.48
8.49
8.40
8.57
8.58
8.63
8.59
8.66
8.68
8.56
8.59
8.62
8.55
8.56
8.50
8.53
8.45
8.46
8.41
8.59
8.60
8.61
8.60
8.60
8.60
3/1/2013 13:21
3/1/2013 13:31
3/1/2013 13:41
3/1/2013 13:51
3/1/2013 14:01
3/1/2013 14:11
3/1/2013 14:21
3/1/2013 14:31
3/1/2013 14:41
3/1/2013 14:51
3/1/2013 15:01
3/1/2013 15:11
3/1/2013 15:21
3/1/2013 15:31
3/1/2013 15:41
3/1/2013 15:51
3/1/2013 16:01
3/1/2013 16:11
3/1/2013 16:21
3/1/2013 16:31
3/1/2013 16:41
3/1/2013 16:51
3/1/2013 17:01
3/1/2013 17:11
3/1/2013 17:21
3/1/2013 17:31
3/1/2013 17:41
3/1/2013 17:51
3/1/2013 18:01
3/1/2013 18:11
3/1/2013 18:21
3/1/2013 18:31
3/1/2013 18:41
3/1/2013 18:51
8.81
8.94
8.40
8.71
9.05
8.38
8.22
8.34
8.50
8.01
8.25
7.95
7.38
8.82
8.05
8.71
8.40
8.16
9.13
7.94
8.18
7.84
7.72
7.68
7.65
7.54
7.44
7.35
7.22
7.19
7.14
7.05
7.01
6.98
5.38
5.23
5.47
5.93
5.77
6.82
5.71
5.85
7.12
5.70
7.32
6.54
4.94
5.22
5.19
5.21
5.20
5.20
5.23
5.19
5.19
5.18
5.18
5.18
5.16
5.17
5.15
5.15
5.14
5.14
5.13
5.11
5.10
5.10
4/15/2013 16:34
4/15/2013 16:44
4/15/2013 16:54
4/15/2013 17:04
4/15/2013 17:14
4/15/2013 17:24
4/15/2013 17:34
4/15/2013 17:44
4/15/2013 17:54
4/15/2013 18:04
4/15/2013 18:14
4/15/2013 18:24
4/15/2013 18:34
4/15/2013 18:44
4/15/2013 18:54
4/15/2013 19:04
4/15/2013 19:14
4/15/2013 19:24
4/15/2013 19:34
4/15/2013 19:44
4/15/2013 19:54
4/15/2013 20:04
4/15/2013 20:14
4/15/2013 20:24
4/15/2013 20:34
4/15/2013 20:44
4/15/2013 20:54
4/15/2013 21:04
4/15/2013 21:14
4/15/2013 21:24
4/15/2013 21:34
4/15/2013 21:44
4/15/2013 21:54
4/15/2013 22:04
9.20
9.14
9.18
9.23
9.15
9.14
9.14
9.10
9.04
8.99
8.94
8.88
8.81
8.74
8.67
8.61
8.53
8.46
8.39
8.33
8.27
8.19
8.13
8.07
8.01
7.95
7.90
7.85
7.80
7.76
7.71
7.67
7.63
7.59
1/26/2013 8:18
1/26/2013 8:28
1/26/2013 8:38
1/26/2013 8:48
1/26/2013 8:58
1/26/2013 9:08
5.44
5.45
5.46
5.47
5.48
5.50
5.61
5.62
5.62
5.63
5.64
5.65
8.60
8.60
8.60
8.60
8.61
8.60
2/8/2013 19:19
2/8/2013 19:29
2/8/2013 19:39
2/8/2013 19:49
2/8/2013 19:59
2/8/2013 20:09
2/8/2013 20:19
2/8/2013 20:29
2/8/2013 20:39
2/8/2013 20:49
2/8/2013 20:59
2/8/2013 21:09
2/8/2013 21:19
2/8/2013 21:29
2/8/2013 21:39
2/8/2013 21:49
2/8/2013 21:59
2/8/2013 22:09
2/8/2013 22:19
2/8/2013 22:29
2/8/2013 22:39
2/8/2013 22:49
2/8/2013 22:59
2/8/2013 23:09
2/8/2013 23:19
2/8/2013 23:29
2/8/2013 23:39
2/8/2013 23:49
2/8/2013 23:59
2/9/2013 0:09
2/9/2013 0:19
2/9/2013 0:29
2/9/2013 0:39
2/9/2013 0:49
6.50
6.48
6.47
6.46
6.44
6.43
6.41
6.41
6.39
6.39
6.39
6.38
6.37
6.37
6.35
6.35
6.34
6.33
6.33
6.32
6.32
6.31
6.31
6.30
6.30
6.29
6.29
6.28
6.28
6.27
6.26
6.26
6.25
6.25
5.86
5.85
5.84
5.82
5.79
5.78
5.78
5.76
5.75
5.75
5.74
5.74
5.73
5.72
5.71
5.71
5.70
5.69
5.68
5.67
5.67
5.66
5.65
5.65
5.64
5.64
5.63
5.63
5.62
5.61
5.61
5.60
5.60
5.59
105
8.59
8.69
8.66
8.67
8.66
8.58
8.74
8.73
8.70
8.68
8.66
8.63
8.64
8.64
8.64
8.64
8.65
8.63
8.62
8.65
8.64
8.64
8.64
8.65
8.61
8.56
8.57
8.59
8.60
8.64
8.63
8.62
8.61
8.60
3/1/2013 19:01 6.89 5.12
3/1/2013 19:11 6.90 5.09
3/1/2013 19:21 6.89 5.08
3/1/2013 19:31 6.90 5.07
3/1/2013 19:41 6.89 4.70
3/1/2013 19:51 6.92 5.07
3/1/2013 20:01 6.91 4.71
3/1/2013 20:11 6.88 4.44
3/1/2013 20:21 6.91 4.73
3/1/2013 20:31 6.90 4.66
3/1/2013 20:41 6.91 4.57
3/1/2013 20:51 6.99 4.79
3/1/2013 21:01 6.99 4.84
3/1/2013 21:11 7.07 4.73
3/1/2013 21:21 7.01 4.55
3/1/2013 21:31 7.06 4.50
3/1/2013 21:41 7.00 4.54
3/1/2013 21:51 6.96 4.64
3/1/2013 22:01 6.97 4.72
3/1/2013 22:11 6.99 4.76
3/1/2013 22:21 6.99 4.74
3/1/2013 22:31 7.01 4.75
3/1/2013 22:41 7.02 4.72
3/1/2013 22:51 7.03 4.74
3/1/2013 23:01 7.06 4.74
3/1/2013 23:11 7.08 4.79
3/1/2013 23:21 7.02 4.69
3/1/2013 23:31 7.06 4.85
3/1/2013 23:41 7.09 4.84
3/1/2013 23:51 7.06 4.74
3/2/2013 0:01 7.08 4.84
3/2/2013 0:11 16.46 7.90
3/2/2013 0:21 10.19 4.69
3/2/2013 0:31 9.97 5.41
4/15/2013 22:14
4/15/2013 22:24
4/15/2013 22:34
4/15/2013 22:44
4/15/2013 22:54
4/15/2013 23:04
4/15/2013 23:14
4/15/2013 23:24
4/15/2013 23:34
4/15/2013 23:44
4/15/2013 23:54
4/16/2013 0:04
4/16/2013 0:14
4/16/2013 0:24
4/16/2013 0:34
4/16/2013 0:44
4/16/2013 0:54
4/16/2013 1:04
4/16/2013 1:14
4/16/2013 1:24
4/16/2013 1:34
4/16/2013 1:44
4/16/2013 1:54
4/16/2013 2:04
4/16/2013 2:14
4/16/2013 2:24
4/16/2013 2:34
4/16/2013 2:44
4/16/2013 2:54
4/16/2013 3:04
4/16/2013 3:14
4/16/2013 3:24
4/16/2013 3:34
4/16/2013 3:44
7.55
7.52
7.49
7.45
7.42
7.39
7.35
7.33
7.30
7.26
7.24
7.22
7.20
7.18
7.16
7.14
7.12
7.11
7.09
7.07
7.06
7.04
7.03
7.01
7.00
6.99
6.98
6.96
6.94
6.93
6.92
6.91
6.89
6.87
2/9/2013 0:59
2/9/2013 1:09
2/9/2013 1:19
2/9/2013 1:29
2/9/2013 1:39
2/9/2013 1:49
2/9/2013 1:59
2/9/2013 2:09
2/9/2013 2:19
2/9/2013 2:29
2/9/2013 2:39
2/9/2013 2:49
2/9/2013 2:59
2/9/2013 3:09
2/9/2013 3:19
2/9/2013 3:29
2/9/2013 3:39
2/9/2013 3:49
2/9/2013 3:59
2/9/2013 4:09
2/9/2013 4:19
2/9/2013 4:29
2/9/2013 4:39
2/9/2013 4:49
2/9/2013 4:59
2/9/2013 5:09
2/9/2013 5:19
2/9/2013 5:29
2/9/2013 5:39
2/9/2013 5:49
2/9/2013 5:59
2/9/2013 6:09
6.24
6.24
6.23
6.22
6.22
6.21
6.21
6.20
6.20
6.19
6.19
6.17
6.17
6.16
6.16
6.15
6.17
6.17
6.17
6.16
6.16
6.14
6.15
6.14
6.14
6.14
6.13
6.13
6.13
6.12
6.12
6.11
5.59
5.58
5.57
5.57
5.57
5.56
5.56
5.55
5.54
5.53
5.52
5.52
5.51
5.51
5.51
5.51
5.51
5.51
5.51
5.50
5.50
5.49
5.49
5.49
5.48
5.48
5.47
5.48
5.47
5.46
5.46
5.45
106
8.63
8.63
8.62
8.60
8.60
8.59
8.56
8.52
8.55
8.54
8.54
8.55
8.53
8.53
8.30
8.53
8.53
8.53
8.55
8.54
8.55
8.55
8.54
8.55
8.55
8.55
8.56
8.54
8.54
8.53
8.54
8.54
3/2/2013 0:41
3/2/2013 0:51
3/2/2013 1:01
3/2/2013 1:11
3/2/2013 1:21
3/2/2013 1:31
3/2/2013 1:41
3/2/2013 1:51
3/2/2013 2:01
3/2/2013 2:11
3/2/2013 2:21
3/2/2013 2:31
3/2/2013 2:41
3/2/2013 2:51
3/2/2013 3:01
3/2/2013 3:11
3/2/2013 3:21
3/2/2013 3:31
3/2/2013 3:41
3/2/2013 3:51
3/2/2013 4:01
3/2/2013 4:11
3/2/2013 4:21
3/2/2013 4:31
3/2/2013 4:41
3/2/2013 4:51
3/2/2013 5:01
3/2/2013 5:11
3/2/2013 5:21
3/2/2013 5:31
3/2/2013 5:41
3/2/2013 5:51
9.61
9.40
9.26
9.05
9.00
8.87
8.77
8.72
8.66
8.60
8.58
8.50
8.46
8.40
8.38
8.36
8.35
8.29
8.23
8.23
8.20
8.19
8.21
8.13
8.07
7.97
8.01
7.93
7.82
7.81
8.08
8.02
5.40
5.38
5.38
5.37
5.36
5.35
5.34
5.34
5.33
5.32
5.31
5.31
5.31
5.31
5.31
5.31
5.31
5.31
5.30
5.30
5.30
5.29
5.29
5.28
5.28
5.28
5.27
5.26
5.27
5.27
5.26
5.26
4/16/2013 3:54
4/16/2013 4:04
4/16/2013 4:14
4/16/2013 4:24
4/16/2013 4:34
4/16/2013 4:44
4/16/2013 4:54
4/16/2013 5:04
4/16/2013 5:14
4/16/2013 5:24
4/16/2013 5:34
4/16/2013 5:44
4/16/2013 5:54
4/16/2013 6:04
4/16/2013 6:14
4/16/2013 6:24
4/16/2013 6:34
4/16/2013 6:44
4/16/2013 6:54
4/16/2013 7:04
4/16/2013 7:14
4/16/2013 7:24
4/16/2013 7:34
4/16/2013 7:44
4/16/2013 7:54
4/16/2013 8:04
4/16/2013 8:14
4/16/2013 8:24
4/16/2013 8:34
4/16/2013 8:44
4/16/2013 8:54
4/16/2013 9:04
6.86
6.85
6.84
6.83
6.82
6.81
6.80
6.79
6.78
6.78
6.77
6.76
6.75
6.75
6.74
6.74
6.74
6.75
6.76
6.78
6.81
6.83
6.81
6.82
6.84
6.86
6.88
6.92
7.01
7.09
7.15
7.20
Appendix 2. (continued)
May
5/30/2013 6:29
5/30/2013 6:39
5/30/2013 6:49
5/30/2013 6:59
5/30/2013 7:09
5/30/2013 7:19
5/30/2013 7:29
5/30/2013 7:39
5/30/2013 7:49
5/30/2013 7:59
5/30/2013 8:09
5/30/2013 8:19
5/30/2013 8:29
5/30/2013 8:39
5/30/2013 8:49
5/30/2013 8:59
5/30/2013 9:09
5/30/2013 9:19
5/30/2013 9:29
5/30/2013 9:39
5/30/2013 9:49
5/30/2013 9:59
5/30/2013 10:09
5/30/2013 10:19
5/30/2013 10:29
5/30/2013 10:39
5/30/2013 10:49
5/30/2013 10:59
5/30/2013 11:09
5/30/2013 11:19
5/30/2013 11:29
5/30/2013 11:39
DO
5.95
5.24
5.06
4.94
4.84
4.80
4.73
4.64
4.64
4.66
4.69
4.69
4.69
4.81
4.84
4.88
4.89
4.94
5.00
5.05
5.11
5.24
5.28
5.38
5.46
5.55
5.63
5.85
5.92
6.06
6.05
6.26
DO
6.09
6.02
5.97
5.93
5.90
5.90
5.86
5.83
5.81
5.81
5.81
5.78
5.73
5.80
5.81
5.83
5.85
5.79
5.81
5.70
5.81
5.82
5.81
5.80
5.78
5.74
5.73
5.64
6.06
6.16
6.18
6.20
pH
7.67
7.66
7.66
7.66
7.66
7.66
7.66
7.65
7.65
7.65
7.65
7.66
7.66
7.66
7.66
7.67
7.67
7.68
7.68
7.68
7.69
7.69
7.70
7.70
7.71
7.72
7.72
7.73
7.73
7.74
7.74
7.76
June
6/17/2013 17:20
6/17/2013 17:30
6/17/2013 17:40
6/17/2013 17:50
6/17/2013 18:00
6/17/2013 18:10
6/17/2013 18:20
6/17/2013 18:30
6/17/2013 18:40
6/17/2013 18:50
6/17/2013 19:00
6/17/2013 19:10
6/17/2013 19:20
6/17/2013 19:30
6/17/2013 19:40
6/17/2013 19:50
6/17/2013 20:00
6/17/2013 20:10
6/17/2013 20:20
6/17/2013 20:30
6/17/2013 20:40
6/17/2013 20:50
6/17/2013 21:00
6/17/2013 21:10
6/17/2013 21:20
6/17/2013 21:30
6/17/2013 21:40
6/17/2013 21:50
6/17/2013 22:00
6/17/2013 22:10
6/17/2013 22:20
6/17/2013 22:30
DO
8.70
8.28
8.33
8.28
8.24
8.11
8.00
8.01
7.92
7.96
7.62
7.79
7.70
7.63
7.49
7.27
7.37
7.16
7.37
7.28
7.22
7.26
7.16
7.07
7.12
7.07
7.05
7.08
7.06
7.07
6.74
6.88
107
DO
6.86
6.73
6.95
7.06
7.34
7.77
7.76
7.43
7.38
7.27
7.02
7.32
7.31
7.42
7.41
7.23
7.02
6.83
7.01
6.90
6.93
7.04
6.98
6.75
6.92
7.06
6.60
6.92
6.76
6.73
6.49
6.69
pH
8.25
8.24
8.24
8.24
8.24
8.24
8.24
8.24
8.24
8.23
8.23
8.23
8.22
8.22
8.22
8.22
8.22
8.21
8.21
8.20
8.19
8.19
8.18
8.18
8.17
8.17
8.17
8.17
8.16
8.14
8.13
8.13
July
7/29/2013 12:03
7/29/2013 12:13
7/29/2013 12:23
7/29/2013 12:33
7/29/2013 12:43
7/29/2013 12:53
7/29/2013 13:03
7/29/2013 13:13
7/29/2013 13:23
7/29/2013 13:33
7/29/2013 13:43
7/29/2013 13:53
7/29/2013 14:03
7/29/2013 14:13
7/29/2013 14:23
7/29/2013 14:33
7/29/2013 14:43
7/29/2013 14:53
7/29/2013 15:03
7/29/2013 15:13
7/29/2013 15:23
7/29/2013 15:33
7/29/2013 15:43
7/29/2013 15:53
7/29/2013 16:03
7/29/2013 16:13
7/29/2013 16:23
7/29/2013 16:33
7/29/2013 16:43
7/29/2013 16:53
7/29/2013 17:03
7/29/2013 17:13
DO
10.66
10.54
10.41
10.31
10.66
11.55
11.65
11.84
11.98
12.03
12.41
12.51
12.45
12.48
11.86
12.61
12.11
12.06
12.11
11.87
12.19
12.52
12.57
13.28
13.27
13.14
11.63
11.51
12.12
11.88
11.90
11.55
DO
15.46
16.10
15.75
15.61
15.72
16.19
16.31
16.62
3.85
2.35
2.20
2.54
2.31
2.57
2.51
2.84
3.04
3.44
3.53
3.48
3.39
3.54
3.54
3.82
3.01
4.18
3.90
4.14
4.33
4.27
4.32
5.27
pH
8.18
8.19
8.24
8.21
8.20
8.23
8.21
8.25
8.31
8.31
8.30
8.28
8.30
8.28
8.28
8.28
8.28
8.22
8.23
8.27
8.22
8.30
8.27
8.30
8.24
8.26
8.21
8.16
8.19
8.23
8.23
8.23
5/30/2013 11:49
5/30/2013 11:59
5/30/2013 12:09
5/30/2013 12:19
5/30/2013 12:29
5/30/2013 12:39
5/30/2013 12:49
5/30/2013 12:59
5/30/2013 13:09
5/30/2013 13:19
5/30/2013 13:29
5/30/2013 13:39
5/30/2013 13:49
5/30/2013 13:59
5/30/2013 14:09
5/30/2013 14:19
5/30/2013 14:29
5/30/2013 14:39
5/30/2013 14:49
5/30/2013 14:59
5/30/2013 15:09
5/30/2013 15:19
5/30/2013 15:29
5/30/2013 15:39
5/30/2013 15:49
5/30/2013 15:59
5/30/2013 16:09
5/30/2013 16:19
5/30/2013 16:29
5/30/2013 16:39
5/30/2013 16:49
5/30/2013 16:59
5/30/2013 17:09
5/30/2013 17:19
6.41
6.49
6.66
6.76
6.89
6.93
7.08
7.19
7.41
7.44
7.73
7.76
7.71
7.83
7.71
7.97
8.11
8.11
8.09
8.19
8.15
8.29
8.43
8.46
8.52
8.34
8.41
8.32
8.20
8.17
8.18
8.18
8.25
8.16
6.21
6.21
6.24
6.24
6.26
6.26
6.26
6.26
6.29
6.27
6.32
6.31
6.28
6.30
6.31
6.34
6.34
6.36
6.34
6.37
6.38
6.39
6.38
6.42
6.43
6.43
6.41
6.44
6.41
6.41
6.41
6.40
6.42
6.42
7.76
7.77
7.78
7.78
7.79
7.80
7.80
7.80
7.82
7.82
7.82
7.83
7.83
7.84
7.83
7.84
7.86
7.87
7.86
7.86
7.87
7.87
7.87
7.88
7.89
7.87
7.87
7.88
7.88
7.87
7.87
7.87
7.87
7.87
6/17/2013 22:40
6/17/2013 22:50
6/17/2013 23:00
6/17/2013 23:10
6/17/2013 23:20
6/17/2013 23:30
6/17/2013 23:40
6/17/2013 23:50
6/18/2013 0:00
6/18/2013 0:10
6/18/2013 0:20
6/18/2013 0:30
6/18/2013 0:40
6/18/2013 0:50
6/18/2013 1:00
6/18/2013 1:10
6/18/2013 1:20
6/18/2013 1:30
6/18/2013 1:40
6/18/2013 1:50
6/18/2013 2:00
6/18/2013 2:10
6/18/2013 2:20
6/18/2013 2:30
6/18/2013 2:40
6/18/2013 2:50
6/18/2013 3:00
6/18/2013 3:10
6/18/2013 3:20
6/18/2013 3:30
6/18/2013 3:40
6/18/2013 3:50
6/18/2013 4:00
6/18/2013 4:10
6.98
7.10
6.75
6.97
6.91
6.94
6.98
6.90
6.92
6.73
6.94
6.89
6.91
6.72
6.71
6.84
6.70
6.72
6.68
6.65
6.52
6.65
6.77
6.77
6.57
6.53
6.65
6.63
6.64
6.52
6.70
6.64
6.54
6.51
108
6.81
6.93
6.90
6.82
6.73
6.96
6.84
6.99
6.85
6.71
6.80
6.75
6.74
6.63
6.73
6.70
6.59
6.61
6.90
6.78
6.77
6.74
6.91
6.87
6.78
6.81
6.87
6.79
6.70
6.64
6.87
6.93
6.73
6.71
8.13
8.15
8.14
8.14
8.14
8.14
8.14
8.14
8.13
8.12
8.13
8.12
8.12
8.12
8.12
8.12
8.11
8.11
8.11
8.11
8.11
8.10
8.10
8.10
8.10
8.10
8.11
8.10
8.10
8.10
8.10
8.10
8.10
8.09
7/29/2013 17:23
7/29/2013 17:33
7/29/2013 17:43
7/29/2013 17:53
7/29/2013 18:03
7/29/2013 18:13
7/29/2013 18:23
7/29/2013 18:33
7/29/2013 18:43
7/29/2013 18:53
7/29/2013 19:03
7/29/2013 19:13
7/29/2013 19:23
7/29/2013 19:33
7/29/2013 19:43
7/29/2013 19:53
7/29/2013 20:03
7/29/2013 20:13
7/29/2013 20:23
7/29/2013 20:33
7/29/2013 20:43
7/29/2013 20:53
7/29/2013 21:03
7/29/2013 21:13
7/29/2013 21:23
7/29/2013 21:33
7/29/2013 21:43
7/29/2013 21:53
7/29/2013 22:03
7/29/2013 22:13
7/29/2013 22:23
7/29/2013 22:33
7/29/2013 22:43
7/29/2013 22:53
11.92
9.43
11.42
11.21
11.11
10.83
10.86
10.66
10.69
10.35
10.18
10.09
9.90
9.67
9.51
9.34
9.17
9.02
8.86
8.73
8.60
8.47
8.29
8.14
7.95
7.73
7.61
7.50
7.49
7.36
7.26
7.01
6.94
6.82
5.34
5.23
5.24
5.16
5.15
5.05
5.02
5.00
5.03
4.86
4.81
4.87
4.70
4.53
4.52
4.41
4.46
4.40
4.35
4.33
4.25
4.13
4.09
3.97
3.86
3.82
3.75
3.70
3.66
3.67
3.66
3.58
3.54
3.65
8.23
8.24
8.24
8.25
8.20
8.21
8.21
8.21
8.23
8.21
8.21
8.19
8.18
8.18
8.18
8.17
8.16
8.13
8.12
8.12
8.12
8.11
8.09
8.08
8.08
8.07
8.06
8.05
8.06
8.05
8.04
8.02
8.02
8.01
5/30/2013 17:29
5/30/2013 17:39
5/30/2013 17:49
5/30/2013 17:59
5/30/2013 18:09
5/30/2013 18:19
5/30/2013 18:29
5/30/2013 18:39
5/30/2013 18:49
5/30/2013 18:59
5/30/2013 19:09
5/30/2013 19:19
5/30/2013 19:29
5/30/2013 19:39
5/30/2013 19:49
5/30/2013 19:59
5/30/2013 20:09
5/30/2013 20:19
5/30/2013 20:29
5/30/2013 20:39
5/30/2013 20:49
5/30/2013 20:59
5/30/2013 21:09
5/30/2013 21:19
5/30/2013 21:29
5/30/2013 21:39
5/30/2013 21:49
5/30/2013 21:59
5/30/2013 22:09
5/30/2013 22:19
5/30/2013 22:29
5/30/2013 22:39
5/30/2013 22:49
5/30/2013 22:59
8.05
8.01
7.87
7.99
7.67
7.56
7.36
7.34
7.33
7.37
7.34
7.19
7.01
6.91
6.81
6.66
6.64
6.65
6.52
6.43
6.34
6.19
6.09
6.05
5.92
5.91
5.84
5.79
5.63
5.63
5.59
5.47
5.41
5.37
6.40
6.41
6.39
6.40
6.36
6.36
6.33
6.32
6.33
6.32
6.32
6.31
6.23
6.27
6.24
6.22
6.21
6.20
6.19
6.17
6.16
6.13
6.13
6.11
6.09
6.10
6.10
6.07
6.07
6.07
6.06
6.03
6.04
6.03
7.87
7.87
7.86
7.86
7.87
7.86
7.86
7.86
7.85
7.85
7.85
7.84
7.83
7.82
7.81
7.80
7.80
7.80
7.79
7.79
7.78
7.77
7.77
7.77
7.76
7.76
7.76
7.75
7.75
7.74
7.73
7.73
7.72
7.72
6/18/2013 4:20
6/18/2013 4:30
6/18/2013 4:40
6/18/2013 4:50
6/18/2013 5:00
6/18/2013 5:10
6/18/2013 5:20
6/18/2013 5:30
6/18/2013 5:40
6/18/2013 5:50
6/18/2013 6:00
6/18/2013 6:10
6/18/2013 6:20
6/18/2013 6:30
6/18/2013 6:40
6/18/2013 6:50
6/18/2013 7:00
6/18/2013 7:10
6/18/2013 7:20
6/18/2013 7:30
6/18/2013 7:40
6/18/2013 7:50
6/18/2013 8:00
6/18/2013 8:10
6/18/2013 8:20
6/18/2013 8:30
6/18/2013 8:40
6/18/2013 8:50
6/18/2013 9:00
6/18/2013 9:10
6/18/2013 9:20
6/18/2013 9:30
6/18/2013 9:40
6/18/2013 9:50
6.50
6.30
6.56
6.47
5.77
6.43
6.56
5.96
6.64
6.22
6.22
5.95
6.28
6.78
6.65
6.33
6.44
6.25
6.15
6.08
6.42
6.34
6.40
6.46
6.50
6.45
6.56
6.41
6.66
6.39
6.48
6.44
6.55
6.45
109
6.67
6.48
6.64
6.65
5.97
6.70
6.54
6.12
6.72
6.48
6.39
6.19
6.40
6.82
6.60
6.53
6.72
6.50
6.59
6.57
6.50
6.46
6.47
6.53
6.58
6.59
6.63
6.48
6.62
6.47
6.59
6.46
6.66
6.41
8.10
8.10
8.10
8.10
8.08
8.09
8.07
8.06
8.08
8.07
8.08
8.06
8.07
8.07
8.06
8.06
8.06
8.06
8.06
8.06
8.06
8.06
8.06
8.05
8.06
8.06
8.06
8.06
8.07
8.07
8.07
8.07
8.08
8.08
7/29/2013 23:03
7/29/2013 23:13
7/29/2013 23:23
7/29/2013 23:33
7/29/2013 23:43
7/29/2013 23:53
7/30/2013 0:03
7/30/2013 0:13
7/30/2013 0:23
7/30/2013 0:33
7/30/2013 0:43
7/30/2013 0:53
7/30/2013 1:03
7/30/2013 1:13
7/30/2013 1:23
7/30/2013 1:33
7/30/2013 1:43
7/30/2013 1:53
7/30/2013 2:03
7/30/2013 2:13
7/30/2013 2:23
7/30/2013 2:33
7/30/2013 2:43
7/30/2013 2:53
7/30/2013 3:03
7/30/2013 3:13
7/30/2013 3:23
7/30/2013 3:33
7/30/2013 3:43
7/30/2013 3:53
7/30/2013 4:03
7/30/2013 4:13
7/30/2013 4:23
7/30/2013 4:33
6.85
6.72
6.68
6.66
6.56
6.55
6.43
6.38
6.33
6.25
6.24
6.19
6.10
6.01
6.01
6.01
5.96
5.93
5.80
5.79
5.69
5.56
5.40
5.36
5.36
5.28
5.29
5.29
5.43
5.40
5.36
5.34
5.34
5.31
3.72
3.68
3.61
3.71
3.76
3.71
3.74
3.74
3.71
3.69
3.71
3.70
3.64
3.63
3.66
3.54
3.60
3.70
3.58
3.67
3.60
3.66
3.74
4.25
4.00
4.26
4.32
4.44
4.79
4.94
4.88
5.23
4.65
4.95
8.00
7.99
7.98
7.98
7.97
8.01
8.00
8.00
8.00
7.97
7.99
7.98
7.97
7.96
7.93
7.95
7.95
7.93
7.93
7.92
7.93
7.92
7.93
7.92
7.92
7.91
7.90
7.88
7.87
7.88
7.88
7.87
7.89
7.88
5/30/2013 23:09
5/30/2013 23:19
5/30/2013 23:29
5/30/2013 23:39
5/30/2013 23:49
5/30/2013 23:59
5/31/2013 0:09
5/31/2013 0:19
5/31/2013 0:29
5/31/2013 0:39
5/31/2013 0:49
5/31/2013 0:59
5/31/2013 1:09
5/31/2013 1:19
5/31/2013 1:29
5/31/2013 1:39
5/31/2013 1:49
5/31/2013 1:59
5/31/2013 2:09
5/31/2013 2:19
5/31/2013 2:29
5/31/2013 2:39
5/31/2013 2:49
5/31/2013 2:59
5/31/2013 3:09
5/31/2013 3:19
5/31/2013 3:29
5/31/2013 3:39
5/31/2013 3:49
5/31/2013 3:59
5/31/2013 4:09
5/31/2013 4:19
5/31/2013 4:29
5/31/2013 4:39
5.34
5.30
5.21
5.20
5.09
5.07
5.08
4.96
4.95
4.90
4.98
4.93
4.95
4.83
4.90
4.92
4.78
4.85
4.78
4.73
4.78
4.76
4.77
4.79
4.73
4.75
4.74
4.69
4.68
4.71
4.73
4.72
4.73
4.67
6.00
6.03
5.99
5.99
5.99
5.98
5.98
5.96
5.95
5.97
5.97
5.97
5.96
5.94
5.96
5.96
5.94
5.93
5.94
5.95
5.93
5.91
5.94
5.94
5.92
5.94
5.93
5.91
5.93
5.93
5.91
5.93
5.93
5.90
7.71
7.71
7.70
7.70
7.70
7.70
7.70
7.70
7.70
7.70
7.70
7.70
7.70
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
6/18/2013 10:00
6/18/2013 10:10
6/18/2013 10:20
6/18/2013 10:30
6/18/2013 10:40
6/18/2013 10:50
6/18/2013 11:00
6/18/2013 11:10
6/18/2013 11:20
6/18/2013 11:30
6/18/2013 11:40
6/18/2013 11:50
6/18/2013 12:00
6/18/2013 12:10
6/18/2013 12:20
6/18/2013 12:30
6/18/2013 12:40
6/18/2013 12:50
6/18/2013 13:00
6/18/2013 13:10
6/18/2013 13:20
6/18/2013 13:30
6/18/2013 13:40
6/18/2013 13:50
6/18/2013 14:00
6/18/2013 14:10
6/18/2013 14:20
6/18/2013 14:30
6/18/2013 14:40
6/18/2013 14:50
6/18/2013 15:00
6/18/2013 15:10
6/18/2013 15:20
6/18/2013 15:30
6.79
6.84
6.80
6.66
6.84
6.89
6.60
7.02
6.72
6.97
6.84
7.32
7.27
7.33
7.57
7.58
7.37
7.82
7.84
7.96
8.21
8.48
8.60
8.51
8.52
8.53
8.56
8.32
8.55
9.27
9.04
9.31
8.89
8.71
110
6.57
6.49
6.57
6.68
6.52
6.65
6.50
6.69
6.68
6.95
6.75
6.86
6.99
6.89
7.13
7.16
7.18
7.22
7.02
7.28
7.30
7.36
7.51
7.31
7.42
7.26
7.31
7.27
7.10
7.26
7.13
7.14
7.30
6.82
8.08
8.07
8.08
8.08
8.09
8.09
8.08
8.09
8.10
8.10
8.10
8.10
8.10
8.10
8.10
8.10
8.11
8.11
8.09
8.10
8.11
8.12
8.12
8.12
8.13
8.12
8.12
8.12
8.12
8.12
8.12
8.12
8.13
8.12
7/30/2013 4:43
7/30/2013 4:53
7/30/2013 5:03
7/30/2013 5:13
7/30/2013 5:23
7/30/2013 5:33
7/30/2013 5:43
7/30/2013 5:53
7/30/2013 6:03
7/30/2013 6:13
7/30/2013 6:23
7/30/2013 6:33
7/30/2013 6:43
7/30/2013 6:53
7/30/2013 7:03
7/30/2013 7:13
7/30/2013 7:23
7/30/2013 7:33
7/30/2013 7:43
7/30/2013 7:53
7/30/2013 8:03
7/30/2013 8:13
7/30/2013 8:23
7/30/2013 8:33
7/30/2013 8:43
7/30/2013 8:53
7/30/2013 9:03
7/30/2013 9:13
7/30/2013 9:23
7/30/2013 9:33
7/30/2013 9:43
7/30/2013 9:53
7/30/2013 10:03
7/30/2013 10:13
5.15
5.25
5.24
3.54
5.19
5.13
5.14
5.13
5.16
5.12
5.10
5.00
5.10
5.11
5.17
5.22
5.33
5.35
5.39
5.15
5.40
5.54
5.56
5.58
5.75
5.72
5.79
6.03
6.08
6.25
6.45
6.61
7.55
7.41
4.55
5.19
4.80
5.00
4.90
4.77
4.92
4.80
4.73
4.53
4.66
4.92
4.59
4.68
4.34
4.42
4.74
4.66
4.46
4.32
3.85
4.11
4.25
3.94
3.88
3.51
3.13
3.48
3.34
3.18
3.12
3.09
3.12
3.01
7.89
7.89
7.88
7.86
7.87
7.86
7.86
7.86
7.85
7.85
7.85
7.86
7.85
7.85
7.86
7.87
7.86
7.85
7.85
7.84
7.83
7.82
7.83
7.82
7.82
7.82
7.83
7.85
7.82
7.82
7.83
7.83
7.84
7.86
5/31/2013 4:49
5/31/2013 4:59
5/31/2013 5:09
5/31/2013 5:19
5/31/2013 5:29
5/31/2013 5:39
5/31/2013 5:49
5/31/2013 5:59
5/31/2013 6:09
5/31/2013 6:19
5/31/2013 6:29
5/31/2013 6:39
5/31/2013 6:49
5/31/2013 6:59
5/31/2013 7:09
5/31/2013 7:19
5/31/2013 7:29
5/31/2013 7:39
5/31/2013 7:49
5/31/2013 7:59
5/31/2013 8:09
5/31/2013 8:19
5/31/2013 8:29
5/31/2013 8:39
5/31/2013 8:49
5/31/2013 8:59
5/31/2013 9:09
5/31/2013 9:19
5/31/2013 9:29
5/31/2013 9:39
5/31/2013 9:49
5/31/2013 9:59
5/31/2013 10:09
5/31/2013 10:19
4.63
4.70
4.65
4.68
4.71
4.46
4.67
4.58
4.59
4.58
4.59
4.59
4.60
4.53
4.62
4.63
4.66
4.60
4.64
4.64
4.65
4.82
4.76
4.78
4.87
4.90
4.84
4.93
4.97
4.84
5.03
5.05
5.04
5.08
5.92
5.93
5.91
5.93
5.90
5.87
5.92
5.92
5.92
5.94
5.93
5.92
5.91
5.90
5.94
5.91
5.94
5.91
5.90
5.93
5.91
5.94
5.95
5.94
5.96
5.97
5.95
5.97
5.97
6.01
6.01
6.01
6.02
6.05
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.69
7.70
7.70
7.70
7.70
7.70
7.70
7.70
7.69
7.69
7.70
7.70
7.70
7.70
7.70
7.71
7.71
7.71
7.71
7.69
7.71
7.71
7.70
7.61
6/18/2013 15:40
6/18/2013 15:50
6/18/2013 16:00
6/18/2013 16:10
6/18/2013 16:20
6/18/2013 16:30
6/18/2013 16:40
6/18/2013 16:50
6/18/2013 17:00
6/18/2013 17:10
6/18/2013 17:20
6/18/2013 17:30
6/18/2013 17:40
6/18/2013 17:50
6/18/2013 18:00
6/18/2013 18:10
6/18/2013 18:20
6/18/2013 18:30
6/18/2013 18:40
6/18/2013 18:50
6/18/2013 19:00
6/18/2013 19:10
6/18/2013 19:20
6/18/2013 19:30
6/18/2013 19:40
6/18/2013 19:50
6/18/2013 20:00
6/18/2013 20:10
6/18/2013 20:20
6/18/2013 20:30
6/18/2013 20:40
6/18/2013 20:50
6/18/2013 21:00
6/18/2013 21:10
8.34
8.13
8.62
9.15
9.55
9.42
9.57
9.33
9.39
9.10
9.16
8.78
8.82
8.85
8.62
8.79
9.20
8.40
8.60
7.97
8.07
8.02
8.15
8.06
7.95
7.69
8.05
8.16
8.45
7.81
7.77
8.13
7.80
7.91
111
7.15
7.00
6.88
6.89
7.10
6.87
6.85
6.89
6.89
6.46
6.66
6.71
6.85
7.11
6.80
6.53
7.00
6.87
7.03
6.71
6.52
6.90
6.92
6.54
6.34
6.68
6.47
7.19
6.98
6.33
6.48
6.86
6.41
6.63
8.13
8.12
8.12
8.13
8.14
8.13
8.13
8.13
8.14
8.13
8.14
8.15
8.16
8.16
8.15
8.14
8.15
8.14
8.14
8.13
8.14
8.15
8.15
8.15
8.14
8.14
8.14
8.14
8.16
8.13
8.14
8.15
8.13
8.13
7/30/2013 10:23
7/30/2013 10:33
7/30/2013 10:43
7/30/2013 10:53
7/30/2013 11:03
7/30/2013 11:13
7/30/2013 11:23
7/30/2013 11:33
7/30/2013 11:43
7/30/2013 11:53
7/30/2013 12:03
7/30/2013 12:13
7/30/2013 12:23
7/30/2013 12:33
7/30/2013 12:43
7/30/2013 12:53
7/30/2013 13:03
7/30/2013 13:13
7/30/2013 13:23
7/30/2013 13:33
7/30/2013 13:43
7/30/2013 13:53
7/30/2013 14:03
7/30/2013 14:13
7/30/2013 14:23
7/30/2013 14:33
7/30/2013 14:43
7/30/2013 14:53
7/30/2013 15:03
7/30/2013 15:13
7/30/2013 15:23
7/30/2013 15:33
7/30/2013 15:43
7/30/2013 15:53
7.36
7.55
8.10
9.58
8.71
8.77
8.67
12.82
10.84
10.33
11.93
15.89
13.08
11.50
11.19
11.41
11.58
11.46
13.33
14.50
10.42
10.38
10.00
10.62
10.71
10.92
10.76
10.66
10.09
10.20
11.61
13.59
13.75
14.99
3.10
2.66
2.60
2.20
2.61
2.47
2.21
2.12
2.37
2.35
2.44
2.15
2.68
2.38
2.47
2.14
2.49
2.13
2.25
2.22
2.13
2.16
2.19
2.31
2.27
2.26
2.37
2.36
2.45
2.57
2.64
2.71
2.79
2.80
7.86
7.87
7.88
7.93
7.91
7.92
7.95
7.96
7.97
7.99
7.99
8.00
8.04
8.04
8.03
8.06
8.07
8.05
8.06
8.06
8.07
8.04
8.03
8.04
8.03
8.05
8.06
8.05
8.03
8.03
8.05
8.05
8.08
8.09
5/31/2013 10:29
5/31/2013 10:39
5/31/2013 10:49
5/31/2013 10:59
5/31/2013 11:09
5/31/2013 11:19
5/31/2013 11:29
5/31/2013 11:39
5/31/2013 11:49
5/31/2013 11:59
5/31/2013 12:09
5/31/2013 12:19
5/31/2013 12:29
5/31/2013 12:39
5/31/2013 12:49
5/31/2013 12:59
5/31/2013 13:09
5/31/2013 13:19
5/31/2013 13:29
5/31/2013 13:39
5/31/2013 13:49
5/31/2013 13:59
5/31/2013 14:09
5/31/2013 14:19
5/31/2013 14:29
5/31/2013 14:39
5/31/2013 14:49
5/31/2013 14:59
5/31/2013 15:09
5/31/2013 15:19
5/31/2013 15:29
5.04
5.07
5.14
5.20
5.18
5.10
5.29
5.58
5.57
5.52
5.40
5.58
5.67
5.62
5.71
5.94
5.87
5.95
5.82
5.97
6.08
6.09
5.95
6.29
6.45
6.53
6.28
6.28
6.23
6.12
6.20
6.14
6.14
6.13
6.22
6.22
6.24
6.27
6.29
6.34
6.28
6.25
6.26
6.27
6.25
6.26
6.27
6.30
6.32
6.30
6.31
6.32
6.31
6.32
6.32
6.19
6.21
6.20
6.20
6.21
6.24
6.22
7.70
7.71
7.72
7.72
7.73
7.74
7.72
7.74
7.73
7.72
7.72
7.72
7.71
7.71
7.72
7.73
7.73
7.72
7.72
7.72
7.73
7.73
7.73
7.73
7.75
7.75
7.73
7.73
7.72
7.71
7.71
6/18/2013 21:20
6/18/2013 21:30
6/18/2013 21:40
6/18/2013 21:50
6/18/2013 22:00
6/18/2013 22:10
6/18/2013 22:20
6/18/2013 22:30
6/18/2013 22:40
6/18/2013 22:50
6/18/2013 23:00
6/18/2013 23:10
6/18/2013 23:20
6/18/2013 23:30
6/18/2013 23:40
6/18/2013 23:50
6/19/2013 0:00
6/19/2013 0:10
6/19/2013 0:20
6/19/2013 0:30
6/19/2013 0:40
6/19/2013 0:50
6/19/2013 1:00
6/19/2013 1:10
6/19/2013 1:20
6/19/2013 1:30
6/19/2013 1:40
6/19/2013 1:50
6/19/2013 2:00
6/19/2013 2:10
6/19/2013 2:20
7.79
7.67
7.63
7.35
7.40
7.39
7.30
7.29
6.97
7.40
7.18
7.27
7.32
7.26
7.25
7.18
7.09
7.09
7.00
6.95
6.85
6.79
6.80
6.77
6.66
6.00
6.65
6.22
6.34
6.43
6.57
112
6.57
6.52
6.56
6.47
6.40
6.46
6.44
6.42
6.43
6.46
6.41
6.36
6.36
6.33
6.41
6.36
6.36
6.32
6.47
6.36
6.36
6.36
6.34
6.36
6.33
6.35
6.31
6.29
6.29
6.26
6.26
8.14
8.14
8.14
8.14
8.15
8.15
8.15
8.15
8.14
8.15
8.15
8.15
8.16
8.16
8.16
8.16
8.17
8.16
8.18
8.18
8.18
8.18
8.18
8.19
8.19
8.19
8.19
8.19
8.19
8.19
8.19
7/30/2013 16:03
7/30/2013 16:13
7/30/2013 16:23
7/30/2013 16:33
7/30/2013 16:43
7/30/2013 16:53
7/30/2013 17:03
7/30/2013 17:13
7/30/2013 17:23
7/30/2013 17:33
7/30/2013 17:43
7/30/2013 17:53
7/30/2013 18:03
7/30/2013 18:13
7/30/2013 18:23
7/30/2013 18:33
7/30/2013 18:43
7/30/2013 18:53
7/30/2013 19:03
7/30/2013 19:13
7/30/2013 19:23
7/30/2013 19:33
7/30/2013 19:43
7/30/2013 19:53
7/30/2013 20:03
7/30/2013 20:13
7/30/2013 20:23
7/30/2013 20:33
7/30/2013 20:43
7/30/2013 20:53
7/30/2013 21:03
13.79
12.46
14.45
13.53
12.28
11.18
10.77
10.61
9.01
10.16
9.93
9.62
9.57
9.46
9.34
9.28
8.32
9.65
9.05
9.03
8.83
8.55
8.32
8.27
8.03
7.81
6.72
7.46
7.25
7.11
7.06
2.83
2.87
2.88
2.89
2.86
2.86
2.90
2.88
2.89
2.91
2.93
2.93
2.93
2.93
2.93
2.96
2.92
2.93
2.90
2.91
2.88
2.89
2.88
2.89
2.90
2.92
2.96
2.98
2.89
2.89
2.88
8.10
8.09
8.10
8.12
8.11
8.11
8.11
8.10
8.10
8.09
8.07
8.06
8.04
8.04
8.03
8.03
8.03
8.03
8.03
8.02
8.01
8.00
7.99
7.99
7.98
7.97
7.96
7.95
7.94
7.93
7.92
Appendix 2. (continued)
August
8/6/2013 11:14
8/6/2013 11:24
8/6/2013 11:34
8/6/2013 11:44
8/6/2013 11:54
8/6/2013 12:04
8/6/2013 12:14
8/6/2013 12:24
8/6/2013 12:34
8/6/2013 12:44
8/6/2013 12:54
8/6/2013 13:04
8/6/2013 13:14
8/6/2013 13:24
8/6/2013 13:34
8/6/2013 13:44
8/6/2013 13:54
8/6/2013 14:04
8/6/2013 14:14
8/6/2013 14:24
8/6/2013 14:34
8/6/2013 14:44
8/6/2013 14:54
8/6/2013 15:04
8/6/2013 15:14
8/6/2013 15:24
8/6/2013 15:34
8/6/2013 15:44
DO
8.65
7.34
7.26
7.21
7.08
7.36
7.48
7.43
7.66
7.83
7.4
7.36
7.18
7.25
7.14
7.06
7
7.25
7.69
7.95
8.06
8.07
8.21
8.72
8.59
8.35
8.31
8.12
DO
9.37
9.65
9.84
10.76
10.46
10.23
10.39
10.36
10.59
10.92
10.34
10.38
10.05
10.23
10.1
10.03
9.96
10.37
10.95
11.06
11.34
11.44
11.68
11.95
12.11
11.86
11.7
11.31
pH
8
7.96
7.96
7.95
7.97
8
8.01
8.02
8.03
8.05
8.03
8.03
8.02
8.02
8.02
8.01
8.02
8.03
8.04
8.05
8.07
8.09
8.1
8.11
8.11
8.11
8.1
8.09
September
9/11/2013 19:27
9/11/2013 19:37
9/11/2013 19:47
9/11/2013 19:57
9/11/2013 20:07
9/11/2013 20:17
9/11/2013 20:27
9/11/2013 20:37
9/11/2013 20:47
9/11/2013 20:57
9/11/2013 21:07
9/11/2013 21:17
9/11/2013 21:27
9/11/2013 21:37
9/11/2013 21:47
9/11/2013 21:57
9/11/2013 22:07
9/11/2013 22:17
9/11/2013 22:27
9/11/2013 22:37
9/11/2013 22:47
9/11/2013 22:57
9/11/2013 23:07
9/11/2013 23:17
9/11/2013 23:27
9/11/2013 23:37
9/11/2013 23:47
9/11/2013 23:57
113
DO
9.54
7.93
7.34
7.03
6.8
6.58
6.53
6.45
6.41
6.38
6.29
6.28
6.18
6.23
6.09
6.08
5.95
6.02
6.07
5.83
5.54
5.47
5.38
5.38
5.2
5.14
5.01
4.93
DO
0.23
0.19
0.17
0.19
0.09
0.07
0.06
0.05
0.07
0.05
0.03
0.03
0.02
0.01
0.01
0.01
0.03
0.01
0
0
0
-0.01
-0.01
-0.01
-0.01
-0.02
-0.01
-0.02
pH
8.44
8.42
8.41
8.38
8.36
8.32
8.33
8.28
8.29
8.29
8.25
8.23
8.23
8.23
8.24
8.24
8.22
8.2
8.2
8.15
8.17
8.15
8.14
8.14
8.13
8.11
8.1
8.08
8/6/2013 15:54
8/6/2013 16:04
8/6/2013 16:14
8/6/2013 16:24
8/6/2013 16:34
8/6/2013 16:44
8/6/2013 16:54
8/6/2013 17:04
8/6/2013 17:14
8/6/2013 17:24
8/6/2013 17:34
8/6/2013 17:44
8/6/2013 17:54
8/6/2013 18:04
8/6/2013 18:14
8/6/2013 18:24
8/6/2013 18:34
8/6/2013 18:44
8/6/2013 18:54
8/6/2013 19:04
8/6/2013 19:14
8/6/2013 19:24
8/6/2013 19:34
8/6/2013 19:44
8/6/2013 19:54
8/6/2013 20:04
8/6/2013 20:14
8/6/2013 20:24
8/6/2013 20:34
8/6/2013 20:44
8/6/2013 20:54
8.09
8.11
8.39
8.45
8.54
8.59
8.52
8.44
8.44
8.5
8.42
8.46
8.43
8.51
8.46
8.34
8.26
8.18
8.07
8.01
7.9
7.95
8.19
8.05
8.11
8.02
7.93
7.96
7.8
7.71
7.55
11.43
11.52
11.78
12.04
11.97
12.08
12.04
11.91
11.91
11.93
11.89
11.94
11.9
11.96
11.94
11.78
11.65
11.56
11.44
11.34
11.24
11.21
11.21
11.16
11.09
11.01
10.73
10.84
10.73
10.62
10.52
8.09
8.09
8.11
8.12
8.12
8.12
8.12
8.12
8.12
8.12
8.12
8.12
8.12
8.12
8.11
8.05
8.12
8.12
8.11
8.11
8.11
8.12
8.11
8.11
8.1
8.1
8.1
8.1
8.09
8.09
8.07
9/12/2013 0:07
9/12/2013 0:17
9/12/2013 0:27
9/12/2013 0:37
9/12/2013 0:47
9/12/2013 0:57
9/12/2013 1:07
9/12/2013 1:17
9/12/2013 1:27
9/12/2013 1:37
9/12/2013 1:47
9/12/2013 1:57
9/12/2013 2:07
9/12/2013 2:17
9/12/2013 2:27
9/12/2013 2:37
9/12/2013 2:47
9/12/2013 2:57
9/12/2013 3:07
9/12/2013 3:17
9/12/2013 3:27
9/12/2013 3:37
9/12/2013 3:47
9/12/2013 3:57
9/12/2013 4:07
9/12/2013 4:17
9/12/2013 4:27
9/12/2013 4:37
9/12/2013 4:47
9/12/2013 4:57
9/12/2013 5:07
114
4.82
4.84
4.69
4.59
4.46
4.48
4.35
4.27
4.13
4.11
4.08
4.09
4
4
4.03
3.93
3.9
3.92
3.82
3.77
3.79
3.7
3.68
3.51
3.53
3.48
3.45
3.59
3.51
3.67
3.72
-0.02
-0.02
-0.03
-0.03
-0.04
-0.03
-0.03
-0.05
-0.04
-0.03
-0.04
-0.04
-0.05
-0.04
-0.05
-0.04
-0.04
-0.06
-0.06
-0.06
-0.07
-0.07
-0.06
-0.06
-0.06
-0.05
-0.05
-0.05
-0.05
-0.06
-0.06
8.07
8.09
8.08
8.07
8.07
8.06
8.05
8.05
8.05
8.05
8.04
8.04
8.04
8.04
8.03
8.03
8.02
8.01
8.01
8
8
7.98
7.97
7.98
7.99
7.98
7.97
7.98
7.99
7.99
7.98
8/6/2013 21:04
8/6/2013 21:14
8/6/2013 21:24
8/6/2013 21:34
8/6/2013 21:44
8/6/2013 21:54
8/6/2013 22:04
8/6/2013 22:14
8/6/2013 22:24
8/6/2013 22:34
8/6/2013 22:44
8/6/2013 22:54
8/6/2013 23:04
8/6/2013 23:14
8/6/2013 23:24
8/6/2013 23:34
8/6/2013 23:44
8/6/2013 23:54
8/7/2013 0:04
8/7/2013 0:14
8/7/2013 0:24
8/7/2013 0:34
8/7/2013 0:44
8/7/2013 0:54
8/7/2013 1:04
8/7/2013 1:14
8/7/2013 1:24
8/7/2013 1:34
8/7/2013 1:44
8/7/2013 1:54
8/7/2013 2:04
7.53
7.44
7.38
7.29
7.19
7.13
6.93
7.1
7.03
6.99
6.85
6.83
6.75
6.63
6.56
6.5
6.44
6.36
6.32
6.25
6.22
6.17
6.11
6.07
6.04
5.96
5.92
5.87
5.84
5.78
5.73
10.35
10.29
10.19
10.1
9.97
9.86
9.75
9.67
9.58
9.52
9.62
9.45
9.29
9.19
9.05
8.94
8.86
8.77
8.72
8.66
8.68
8.62
8.52
8.44
8.43
8.38
8.27
8.23
8.15
8.11
8.07
8.07
8.06
8.05
8.05
8.04
8.03
8.03
8.03
8.02
8.01
8.01
8
7.99
7.99
7.98
7.98
7.97
7.96
7.96
7.95
7.95
7.94
7.94
7.93
7.93
7.93
7.92
7.91
7.91
7.91
7.9
9/12/2013 5:17
9/12/2013 5:27
9/12/2013 5:37
9/12/2013 5:47
9/12/2013 5:57
9/12/2013 6:07
9/12/2013 6:17
9/12/2013 6:27
9/12/2013 6:37
9/12/2013 6:47
9/12/2013 6:57
9/12/2013 7:07
9/12/2013 7:17
9/12/2013 7:27
9/12/2013 7:37
9/12/2013 7:47
9/12/2013 7:57
9/12/2013 8:07
9/12/2013 8:17
9/12/2013 8:27
9/12/2013 8:37
9/12/2013 8:47
9/12/2013 8:57
9/12/2013 9:07
9/12/2013 9:17
9/12/2013 9:27
9/12/2013 9:37
9/12/2013 9:47
9/12/2013 9:57
9/12/2013 10:07
9/12/2013 10:17
115
3.67
3.63
3.52
3.62
3.48
3.43
3.47
3.46
0.58
4.11
3.23
3.93
2.82
4.12
3.3
3.95
4.44
4.48
4.39
4.82
5
5.33
5.58
5.55
5.43
5.59
6.02
6.13
5.61
5.18
5.15
-0.06
-0.07
-0.06
-0.07
-0.07
-0.06
-0.06
-0.06
-0.07
-0.08
-0.07
-0.07
-0.07
0.41
0.55
-0.07
-0.07
-0.04
-0.07
5.41
5.52
5.86
6.1
6.04
5.92
6.06
6.49
6.65
6.16
5.83
5.57
7.97
7.96
7.96
7.97
7.96
7.96
7.96
7.96
7.95
7.96
7.94
7.94
7.93
7.92
7.91
7.9
7.9
7.93
7.94
7.99
8.01
8.03
8.05
8.05
8.04
8.05
8.09
8.11
8.07
8.03
7.88
8/7/2013 2:14
8/7/2013 2:24
8/7/2013 2:34
8/7/2013 2:44
8/7/2013 2:54
8/7/2013 3:04
8/7/2013 3:14
8/7/2013 3:24
8/7/2013 3:34
8/7/2013 3:44
8/7/2013 3:54
8/7/2013 4:04
8/7/2013 4:14
8/7/2013 4:24
8/7/2013 4:34
8/7/2013 4:44
8/7/2013 4:54
8/7/2013 5:04
8/7/2013 5:14
8/7/2013 5:24
8/7/2013 5:34
8/7/2013 5:44
8/7/2013 5:54
8/7/2013 6:04
8/7/2013 6:14
8/7/2013 6:24
8/7/2013 6:34
8/7/2013 6:44
8/7/2013 6:54
8/7/2013 7:04
8/7/2013 7:14
5.7
5.42
5.4
5.25
5.28
5.26
5.25
5.16
5.2
5.07
5.03
4.99
4.93
4.88
4.87
4.76
4.83
4.72
4.75
4.73
4.65
4.64
4.67
4.61
4.64
4.61
4.58
4.66
4.66
4.71
4.85
8.04
8.03
7.94
7.89
7.86
7.83
7.79
7.72
7.69
7.68
7.66
7.6
7.6
7.53
7.45
7.51
7.46
7.43
7.36
7.35
7.31
7.32
7.2
7.25
6.98
6.96
6.95
6.88
6.79
6.84
6.95
7.9
7.9
7.89
7.89
7.88
7.88
7.88
7.87
7.87
7.87
7.86
7.85
7.85
7.85
7.84
7.84
7.84
7.83
7.83
7.83
7.83
7.82
7.82
7.82
7.81
7.82
7.82
7.82
7.82
7.81
7.81
9/12/2013 10:27
9/12/2013 10:37
9/12/2013 10:47
9/12/2013 10:57
9/12/2013 11:07
9/12/2013 11:17
9/12/2013 11:27
9/12/2013 11:37
9/12/2013 11:47
9/12/2013 11:57
9/12/2013 12:07
9/12/2013 12:17
9/12/2013 12:27
9/12/2013 12:37
9/12/2013 12:47
9/12/2013 12:57
9/12/2013 13:07
9/12/2013 13:17
9/12/2013 13:27
9/12/2013 13:37
9/12/2013 13:47
9/12/2013 13:57
9/12/2013 14:07
9/12/2013 14:17
9/12/2013 14:27
9/12/2013 14:37
9/12/2013 14:47
9/12/2013 14:57
9/12/2013 15:07
9/12/2013 15:17
9/12/2013 15:27
116
5.02
0.71
0.36
5.12
4.34
4.87
5.04
5.05
5.08
5.15
5.19
5.18
5.21
5.27
5.33
5.37
5.43
5.49
5.53
5.49
5.5
5.53
5.59
5.54
5.42
5.17
5.06
5.05
5.02
4.97
4.98
5.66
5.64
4.29
5.74
5.73
5.67
5.71
5.55
5.5
5.48
5.53
5.55
5.56
5.55
5.56
5.62
5.74
5.77
5.8
5.87
5.88
5.89
5.88
5.89
6.29
5.3
5.39
5.35
5.5
5.49
5.47
7.82
7.83
7.75
7.74
7.71
7.67
7.17
7.65
7.65
7.65
7.66
7.66
7.66
7.68
7.68
7.66
7.65
7.63
7.65
7.66
7.65
7.64
7.63
7.62
7.62
7.59
7.58
7.57
7.55
7.53
7.52
8/7/2013 7:24
8/7/2013 7:34
8/7/2013 7:44
8/7/2013 7:54
8/7/2013 8:04
8/7/2013 8:14
8/7/2013 8:24
8/15/2013 20:10
8/15/2013 20:20
8/15/2013 20:30
8/15/2013 20:40
8/15/2013 20:50
8/15/2013 21:00
8/15/2013 21:10
8/15/2013 21:20
8/15/2013 21:30
8/15/2013 21:40
8/15/2013 21:50
8/15/2013 22:00
8/15/2013 22:10
8/15/2013 22:20
8/15/2013 22:30
8/15/2013 22:40
8/15/2013 22:50
8/15/2013 23:00
8/15/2013 23:10
8/15/2013 23:20
8/15/2013 23:30
8/15/2013 23:40
8/15/2013 23:50
8/16/2013 0:00
4.81
4.85
4.89
4.9
5.07
5.08
5.28
6.13
3.83
1.36
1.13
1.89
0.7
0.9
0.79
0.25
0.47
0.35
0.1
-0.05
-0.04
-0.26
-0.04
-0.25
-0.01
0.06
0.26
-0.26
0.87
-0.21
0.93
7.06
7.17
7.26
7.32
7.39
7.37
7.61
8.97
7.87
8.52
7.47
7.85
8.08
8.29
7.7
8.03
7.82
7.75
6.75
7.26
7.76
7.62
7.14
7.23
7.58
6.85
6.51
6.75
6.95
6.56
6.89
7.82
7.82
7.82
7.82
7.82
7.82
7.83
6.92
7.28
8.41
8.46
8.43
8.45
8.4
8.36
8.41
8.34
8.39
8.37
8.37
8.36
8.37
8.34
8.35
8.31
8.32
8.31
8.31
8.29
8.29
8.29
9/12/2013 15:37
9/12/2013 15:47
9/12/2013 15:57
9/12/2013 16:07
9/12/2013 16:17
9/12/2013 16:27
9/12/2013 16:37
9/12/2013 16:47
9/12/2013 16:57
9/12/2013 17:07
9/12/2013 17:17
9/12/2013 17:27
9/12/2013 17:37
9/12/2013 17:47
9/12/2013 17:57
9/12/2013 18:07
9/12/2013 18:17
9/12/2013 18:27
9/12/2013 18:37
9/12/2013 18:47
9/12/2013 18:57
9/12/2013 19:07
9/12/2013 19:17
9/12/2013 19:27
9/12/2013 19:37
9/12/2013 19:47
9/12/2013 19:57
9/12/2013 20:07
9/12/2013 20:17
9/12/2013 20:27
9/12/2013 20:37
117
4.94
4.94
4.88
4.9
4.84
4.77
4.76
4.78
4.75
4.78
4.74
4.72
4.79
4.83
4.86
4.8
4.71
4.66
4.71
4.61
4.66
4.61
4.58
4.45
4.55
4.47
4.4
4.3
4.23
4.12
4.03
5.38
5.42
4.64
4.72
4.69
4.68
4.65
4.71
4.49
4.46
4.48
4.39
4.44
4.48
4.66
4.71
4.73
4.75
4.7
4.98
5.06
4.89
4.9
4.76
4.61
4.66
4.53
4.35
4.3
4.35
4.42
7.48
7.48
7.43
7.42
7.41
7.37
7.37
7.37
7.36
7.34
7.33
7.34
7.33
7.32
7.32
7.29
7.3
7.31
7.3
7.28
7.28
7.28
7.27
7.27
7.26
7.23
7.22
7.22
7.2
7.19
7.17
8/16/2013 0:10
8/16/2013 0:20
8/16/2013 0:30
8/16/2013 0:40
8/16/2013 0:50
8/16/2013 1:00
8/16/2013 1:10
8/16/2013 1:20
8/16/2013 1:30
8/16/2013 1:40
8/16/2013 1:50
8/16/2013 2:00
8/16/2013 2:10
8/16/2013 2:20
8/16/2013 2:30
8/16/2013 2:40
8/16/2013 2:50
8/16/2013 3:00
8/16/2013 3:10
8/16/2013 3:20
8/16/2013 3:30
8/16/2013 3:40
8/16/2013 3:50
8/16/2013 4:00
8/16/2013 4:10
8/16/2013 4:20
8/16/2013 4:30
8/16/2013 4:40
8/16/2013 4:50
8/16/2013 5:00
8/16/2013 5:10
-0.08
-0.36
-0.4
-0.42
-0.41
-0.29
-0.25
-0.39
-0.29
-0.44
-0.34
-0.43
-0.2
-0.27
-0.48
-0.3
-0.42
-0.29
-0.35
0.57
-0.49
-0.16
-0.3
0.38
0.3
0.87
0.29
0.22
0.07
0.32
0.6
6.92
7.14
7.03
6.03
5.33
6.77
6.2
6.7
6.83
6.74
6.51
6.53
6.62
6.58
6.43
6.5
6.49
6.27
6.44
6.18
6.31
6.35
6.25
6.13
6.16
6.23
6.19
6.1
6.14
6.03
6.07
8.25
8.23
8.14
8.14
8.25
8.27
8.25
8.24
8.24
8.24
8.23
8.22
8.22
8.21
8.21
8.2
8.2
8.2
8.2
8.19
8.18
8.17
8.17
8.15
8.17
8.17
8.15
8.13
8.1
8.03
8.11
9/12/2013 20:47
9/12/2013 20:57
9/12/2013 21:07
9/12/2013 21:17
9/12/2013 21:27
9/12/2013 21:37
9/12/2013 21:47
9/12/2013 21:57
9/12/2013 22:07
9/12/2013 22:17
9/12/2013 22:27
9/12/2013 22:37
9/12/2013 22:47
9/12/2013 22:57
9/12/2013 23:07
9/12/2013 23:17
9/12/2013 23:27
9/12/2013 23:37
9/12/2013 23:47
9/12/2013 23:57
9/13/2013 0:07
9/13/2013 0:17
9/13/2013 0:27
9/13/2013 0:37
9/13/2013 0:47
9/13/2013 0:57
9/13/2013 1:07
9/13/2013 1:17
9/13/2013 1:27
9/13/2013 1:37
9/13/2013 1:47
118
3.97
3.98
4.14
3.92
4
4.04
3.85
3.51
3.71
3.45
3.6
3.46
3.44
3.55
3.45
3.36
3.39
3.19
3.26
3.2
3.07
2.88
2.99
2.99
3
2.98
3.03
3.06
3.01
2.99
3.26
4.47
4.46
4.39
4.35
4.35
4.32
4.32
4.37
4.33
4.31
4.28
4.17
4.11
4.07
4
3.9
3.8
3.7
3.56
3.66
3.59
3.56
3.61
3.57
3.58
3.51
3.57
3.47
3.47
3.48
3.6
7.13
7.03
6.96
6.98
6.97
6.96
6.96
6.98
6.99
6.99
7.01
7.03
7.02
7.03
7.02
6.98
6.97
6.96
6.96
6.97
6.97
6.97
6.97
6.97
6.98
6.99
7
7.01
7.02
7.03
7.03
8/16/2013 5:20
8/16/2013 5:30
8/16/2013 5:40
8/16/2013 5:50
8/16/2013 6:00
8/16/2013 6:10
8/16/2013 6:20
8/16/2013 6:30
8/16/2013 6:40
8/16/2013 6:50
8/16/2013 7:00
8/16/2013 7:10
8/16/2013 7:20
8/16/2013 7:30
8/16/2013 7:40
8/16/2013 7:50
1.04
0.67
1.08
1.39
0.39
0.31
1.28
1.09
1.97
1.57
1.35
1.94
1.03
1.54
2.39
3.87
6.07
6.05
6.07
6.04
5.97
5.93
5.94
5.88
5.95
5.95
5.89
5.96
5.94
5.97
6
6.13
8.13
8.12
8.13
8.12
8.12
8.13
8.13
8.11
8.12
8.1
8.11
8.11
8.09
8.09
8.09
8.09
9/13/2013 1:57
9/13/2013 2:07
9/13/2013 2:17
9/13/2013 2:27
9/13/2013 2:37
9/13/2013 2:47
9/13/2013 2:57
9/13/2013 3:07
9/13/2013 3:17
9/13/2013 3:27
9/13/2013 3:37
9/13/2013 3:47
9/13/2013 3:57
9/13/2013 4:07
9/13/2013 4:17
9/13/2013 4:27
3.05
3
3.13
3.11
2.89
2.69
2.85
2.99
2.94
2.76
2.83
3.02
2.61
2.8
2.41
2.37
3.59
3.7
3.7
3.7
3.79
3.86
3.78
3.76
3.74
3.76
3.73
3.71
3.79
3.77
3.82
3.72
7.03
7
7
7.25
7.43
7.55
7.64
7.69
7.69
7.71
7.72
7.75
7.75
7.74
7.73
7.73
Appendix 2. (continued)
October
10/21/13 18:23
10/21/13 18:33
10/21/13 18:43
10/21/13 18:53
10/21/13 19:03
10/21/13 19:13
10/21/13 19:23
10/21/13 19:33
10/21/13 19:43
10/21/13 19:53
10/21/13 20:03
10/21/13 20:13
10/21/13 20:23
DO
8.91
7.85
7.46
7.28
7.18
7.09
7.02
6.91
6.84
6.82
6.77
6.64
6.61
DO
7.29
7.18
7.10
7.03
6.96
6.87
6.81
6.74
6.70
6.64
6.57
6.52
6.46
pH
8.49
8.49
8.63
8.56
8.50
8.67
8.61
8.59
8.56
8.55
8.54
8.53
8.52
November
11/8/2013 16:36
11/8/2013 16:46
11/8/2013 16:56
11/8/2013 17:06
11/8/2013 17:16
11/8/2013 17:26
11/8/2013 17:36
11/8/2013 17:46
11/8/2013 17:56
11/8/2013 18:06
11/8/2013 18:16
11/8/2013 18:26
11/8/2013 18:36
DO
7.80
6.35
5.91
5.77
5.66
5.62
5.29
5.34
5.42
5.55
5.54
5.48
5.43
119
DO
6.52
6.45
6.43
6.44
6.42
6.39
6.38
6.34
6.32
6.29
6.26
6.22
6.17
pH
8.60
8.61
8.62
8.62
8.62
8.62
8.63
8.63
8.65
8.65
8.65
8.65
8.65
December
12/13/2013 16:44
12/13/2013 16:54
12/13/2013 17:04
12/13/2013 17:14
12/13/2013 17:24
12/13/2013 17:34
12/13/2013 17:44
12/13/2013 17:54
12/13/2013 18:04
12/13/2013 18:14
12/13/2013 18:24
12/13/2013 18:34
12/13/2013 18:44
DO
8.96
6.93
6.37
6.11
5.91
5.76
5.64
5.56
5.49
5.39
5.34
5.27
5.22
DO
5.61
5.57
5.44
5.45
5.21
5.01
4.95
4.97
4.93
4.97
4.84
4.82
4.79
pH
8.70
8.69
8.71
8.71
8.70
8.70
8.71
8.70
8.67
8.64
8.65
8.64
8.64
10/21/13 20:33
10/21/13 20:43
10/21/13 20:53
10/21/13 21:03
10/21/13 21:13
10/21/13 21:23
10/21/13 21:33
10/21/13 21:43
10/21/13 21:53
10/21/13 22:03
10/21/13 22:13
10/21/13 22:23
10/21/13 22:33
10/21/13 22:43
10/21/13 22:53
10/21/13 23:03
10/21/13 23:13
10/21/13 23:23
10/21/13 23:33
10/21/13 23:43
10/21/13 23:53
10/22/13 0:03
10/22/13 0:13
10/22/13 0:23
10/22/13 0:33
10/22/13 0:43
10/22/13 0:53
10/22/13 1:03
10/22/13 1:13
10/22/13 1:23
10/22/13 1:33
10/22/13 1:43
10/22/13 1:53
10/22/13 2:03
6.53
6.47
6.40
6.30
6.30
6.27
6.19
6.14
6.08
5.95
5.96
5.88
5.84
5.81
5.73
5.62
5.61
5.54
5.49
5.44
5.36
5.34
5.28
5.26
5.22
5.15
5.10
5.08
5.03
4.98
4.92
4.89
4.83
4.82
6.42
6.28
6.28
6.23
6.19
6.14
6.09
6.03
5.97
5.92
5.88
5.83
5.78
5.73
5.67
5.63
5.59
5.55
5.52
5.48
5.44
5.41
5.36
5.32
5.29
5.26
5.24
5.20
5.17
5.14
5.12
5.09
5.05
5.02
8.50
8.49
8.48
8.43
8.47
8.46
8.44
8.43
8.42
8.37
8.37
8.36
8.35
8.34
8.33
8.32
8.32
8.32
8.31
8.30
8.31
8.30
8.30
8.30
8.29
8.29
8.29
8.26
8.26
8.25
8.27
8.25
8.25
8.25
11/8/2013 18:46
11/8/2013 18:56
11/8/2013 19:06
11/8/2013 19:16
11/8/2013 19:26
11/8/2013 19:36
11/8/2013 19:46
11/8/2013 19:56
11/8/2013 20:06
11/8/2013 20:16
11/8/2013 20:26
11/8/2013 20:36
11/8/2013 20:46
11/8/2013 20:56
11/8/2013 21:06
11/8/2013 21:16
11/8/2013 21:26
11/8/2013 21:36
11/8/2013 21:46
11/8/2013 21:56
11/8/2013 22:06
11/8/2013 22:16
11/8/2013 22:26
11/8/2013 22:36
11/8/2013 22:46
11/8/2013 22:56
11/8/2013 23:06
11/8/2013 23:16
11/8/2013 23:26
11/8/2013 23:36
11/8/2013 23:46
11/8/2013 23:56
11/9/2013 0:06
11/9/2013 0:16
5.21
5.26
5.15
5.05
4.97
4.96
4.95
4.92
4.97
4.86
4.77
4.71
4.56
4.54
4.36
4.39
4.39
3.90
3.38
3.42
3.68
3.96
3.89
4.20
4.00
4.04
4.00
4.00
3.98
4.04
4.04
4.02
4.03
4.02
120
6.13
6.07
6.03
5.97
5.93
5.88
5.84
5.79
5.74
5.69
5.64
5.60
5.55
5.50
5.48
5.44
5.41
5.33
5.33
5.29
5.26
5.23
5.21
5.19
5.15
5.14
5.11
5.08
5.05
5.03
5.02
5.00
4.99
4.97
8.64
8.63
8.62
8.61
8.61
8.59
8.59
8.58
8.58
8.57
8.56
8.56
8.55
8.47
8.44
8.47
8.49
8.50
8.46
8.52
8.51
8.51
8.50
8.41
8.39
8.48
8.46
8.48
8.48
8.48
8.47
8.48
8.48
8.48
12/13/2013 18:54
12/13/2013 19:04
12/13/2013 19:14
12/13/2013 19:24
12/13/2013 19:34
12/13/2013 19:44
12/13/2013 19:54
12/13/2013 20:04
12/13/2013 20:14
12/13/2013 20:24
12/13/2013 20:34
12/13/2013 20:44
12/13/2013 20:54
12/13/2013 21:04
12/13/2013 21:14
12/13/2013 21:24
12/13/2013 21:34
12/13/2013 21:44
12/13/2013 21:54
12/13/2013 22:04
12/13/2013 22:14
12/13/2013 22:24
12/13/2013 22:34
12/13/2013 22:44
12/13/2013 22:54
12/13/2013 23:04
12/13/2013 23:14
12/13/2013 23:24
12/13/2013 23:34
12/13/2013 23:44
12/13/2013 23:54
12/14/2013 0:04
12/14/2013 0:14
12/14/2013 0:24
5.16
5.11
5.11
5.05
4.97
4.96
4.93
4.86
4.79
4.67
4.52
4.52
4.60
4.51
4.49
4.46
4.42
4.58
4.48
4.38
4.26
4.19
4.13
4.06
4.01
3.94
3.89
3.84
3.82
3.76
3.74
3.69
3.64
3.65
4.77
4.71
5.38
5.36
5.33
5.27
5.20
5.20
5.17
3.00
3.01
3.13
3.20
3.27
3.32
3.35
3.38
3.34
3.36
3.38
3.42
3.42
3.40
3.38
3.39
3.41
3.38
3.37
3.36
3.33
3.32
3.32
3.31
3.29
8.62
8.59
8.59
8.57
8.56
8.63
8.59
8.56
8.49
8.41
8.37
8.35
8.34
8.32
8.33
8.31
8.28
8.26
8.24
8.22
8.21
8.19
8.17
8.16
8.12
8.11
8.11
8.30
8.42
8.37
8.42
8.39
8.41
8.39
10/22/13 2:13
10/22/13 2:23
10/22/13 2:33
10/22/13 2:43
10/22/13 2:53
10/22/13 3:03
10/22/13 3:13
10/22/13 3:23
10/22/13 3:33
10/22/13 3:43
10/22/13 3:53
10/22/13 4:03
10/22/13 4:13
10/22/13 4:23
10/22/13 4:33
10/22/13 4:43
10/22/13 4:53
10/22/13 5:03
10/22/13 5:13
10/22/13 5:23
10/22/13 5:33
10/22/13 5:43
10/22/13 5:53
10/22/13 6:03
10/22/13 6:13
10/22/13 6:23
10/22/13 6:33
10/22/13 6:43
10/22/13 6:53
10/22/13 7:03
10/22/13 7:13
10/22/13 7:23
10/22/13 7:33
10/22/13 7:43
4.76
4.71
4.69
4.65
4.58
4.58
4.56
4.52
4.54
4.50
4.48
4.47
4.41
4.36
4.36
4.34
4.31
4.28
4.26
4.24
4.21
4.22
4.22
4.22
4.20
4.23
4.23
4.25
4.24
4.21
4.22
4.18
4.20
4.18
5.00
4.98
4.95
4.93
4.91
4.89
4.87
4.86
4.84
4.83
4.81
4.80
4.78
4.75
4.74
4.73
4.72
4.71
4.70
4.69
4.69
4.68
4.67
4.66
4.65
4.65
4.64
4.63
4.62
4.62
4.61
4.61
4.60
4.60
8.24
8.24
8.24
8.23
8.23
8.22
8.21
8.22
8.22
8.22
8.21
8.21
8.21
8.21
8.21
8.21
8.21
8.21
8.21
8.21
8.20
8.20
8.20
8.20
8.20
8.19
8.20
8.20
8.20
8.19
8.20
8.20
8.20
8.20
11/9/2013 0:26
11/9/2013 0:36
11/9/2013 0:46
11/9/2013 0:56
11/9/2013 1:06
11/9/2013 1:16
11/9/2013 1:26
11/9/2013 1:36
11/9/2013 1:46
11/9/2013 1:56
11/9/2013 2:06
11/9/2013 2:16
11/9/2013 2:26
11/9/2013 2:36
11/9/2013 2:46
11/9/2013 2:56
11/9/2013 3:06
11/9/2013 3:16
11/9/2013 3:26
11/9/2013 3:36
11/9/2013 3:46
11/9/2013 3:56
11/9/2013 4:06
11/9/2013 4:16
11/9/2013 4:26
11/9/2013 4:36
11/9/2013 4:46
11/9/2013 4:56
11/9/2013 5:06
11/9/2013 5:16
11/9/2013 5:26
11/9/2013 5:36
11/9/2013 5:46
11/9/2013 5:56
3.78
3.74
3.52
3.78
3.66
3.60
3.62
3.62
3.55
3.52
3.35
3.29
3.29
3.32
3.29
3.29
3.33
3.32
3.31
3.14
3.04
3.16
3.19
3.05
2.73
3.10
3.05
2.93
2.69
2.45
3.10
2.96
3.05
2.73
121
4.96
4.94
4.93
4.92
4.90
4.89
4.87
4.86
4.85
4.84
4.78
4.74
4.70
4.66
4.67
4.65
4.66
4.66
4.62
4.62
4.61
4.55
4.52
4.57
4.52
4.49
4.49
4.41
4.47
4.47
4.40
4.47
4.39
4.46
8.47
8.47
8.46
8.45
8.46
8.46
8.46
8.45
8.45
8.45
8.45
8.45
8.45
8.44
8.44
8.44
8.43
8.42
8.40
8.37
8.35
8.36
8.37
8.35
8.36
8.40
8.41
8.41
8.40
8.38
8.37
8.35
8.39
8.41
12/14/2013 0:34
12/14/2013 0:44
12/14/2013 0:54
12/14/2013 1:04
12/14/2013 1:14
12/14/2013 1:24
12/14/2013 1:34
12/14/2013 1:44
12/14/2013 1:54
12/14/2013 2:04
12/14/2013 2:14
12/14/2013 2:24
12/14/2013 2:34
12/14/2013 2:44
12/14/2013 2:54
12/14/2013 3:04
12/14/2013 3:14
12/14/2013 3:24
12/14/2013 3:34
12/14/2013 3:44
12/14/2013 3:54
12/14/2013 4:04
12/14/2013 4:14
12/14/2013 4:24
12/14/2013 4:34
12/14/2013 4:44
12/14/2013 4:54
12/14/2013 5:04
12/14/2013 5:14
12/14/2013 5:24
12/14/2013 5:34
12/14/2013 5:44
12/14/2013 5:54
12/14/2013 6:04
3.72
3.57
3.69
3.59
3.57
3.69
3.45
3.54
3.59
3.50
3.36
3.32
3.62
3.45
3.45
3.47
3.40
3.38
3.49
3.39
3.10
3.27
3.31
3.17
3.63
3.43
3.14
3.29
3.22
3.34
3.22
3.26
3.25
3.14
3.27
3.27
3.27
3.27
3.27
3.29
3.33
3.34
3.33
3.33
3.33
3.33
3.34
3.38
3.40
3.39
4.25
4.28
4.29
4.28
4.26
4.25
4.26
4.27
4.21
4.21
4.22
4.22
4.20
4.19
4.18
4.19
4.18
4.18
8.32
8.24
8.03
8.02
8.04
8.07
8.24
8.25
8.23
8.27
8.23
8.20
8.22
8.27
8.24
8.23
8.16
8.32
8.33
7.99
8.00
8.20
8.10
7.99
8.12
8.11
8.10
8.14
8.07
7.93
7.96
8.00
8.07
8.04
10/22/13 7:53
10/22/13 8:03
10/22/13 8:13
10/22/13 8:23
10/22/13 8:33
10/22/13 8:43
10/22/13 8:53
10/22/13 9:03
10/22/13 9:13
10/22/13 9:23
10/22/13 9:33
10/22/13 9:43
10/22/13 9:53
10/22/13 10:03
10/22/13 10:13
10/22/13 10:23
10/22/13 10:33
10/22/13 10:43
10/22/13 10:53
10/22/13 11:03
10/22/13 11:13
10/22/13 11:23
10/22/13 11:33
10/22/13 11:43
10/22/13 11:53
10/22/13 12:03
10/22/13 12:13
10/22/13 12:23
10/22/13 12:33
10/22/13 12:43
10/22/13 12:53
10/22/13 13:03
10/22/13 13:13
10/22/13 13:23
4.12
4.11
4.12
4.11
4.13
4.14
4.17
4.23
4.24
4.28
4.32
4.41
4.38
4.41
4.42
4.47
4.50
4.55
4.61
4.65
4.69
4.73
4.82
4.92
4.99
5.12
5.21
5.27
5.29
5.38
5.45
5.53
5.60
5.68
4.59
4.59
4.60
4.60
4.61
4.64
4.65
4.68
4.72
4.76
4.83
4.87
4.92
4.96
5.00
5.04
5.08
5.11
5.16
5.21
5.25
5.32
5.42
5.49
5.58
5.68
5.77
5.84
5.91
6.03
6.07
6.15
6.23
6.32
8.21
8.18
8.22
8.21
8.21
8.22
8.22
8.23
8.23
8.23
8.23
8.24
8.25
8.25
8.25
8.25
8.25
8.25
8.26
8.26
8.26
8.27
8.28
8.29
8.30
8.30
8.31
8.32
8.32
8.33
8.34
8.34
8.35
8.36
11/9/2013 6:06
11/9/2013 6:16
11/9/2013 6:26
11/9/2013 6:36
11/9/2013 6:46
11/9/2013 6:56
11/9/2013 7:06
11/9/2013 7:16
11/9/2013 7:26
11/9/2013 7:36
11/9/2013 7:46
11/9/2013 7:56
11/9/2013 8:06
11/9/2013 8:16
11/9/2013 8:26
11/9/2013 8:36
11/9/2013 8:46
11/9/2013 8:56
11/9/2013 9:06
11/9/2013 9:16
11/9/2013 9:26
11/9/2013 9:36
11/9/2013 9:46
11/9/2013 9:56
11/9/2013 10:06
11/9/2013 10:16
11/9/2013 10:26
11/9/2013 10:36
11/9/2013 10:46
11/9/2013 10:56
11/9/2013 11:06
11/9/2013 11:16
11/9/2013 11:26
11/9/2013 11:36
3.25
3.25
3.48
3.51
3.41
3.35
3.37
3.36
3.33
3.30
3.28
3.32
3.41
3.42
3.43
3.46
3.45
3.49
3.52
3.57
3.61
3.58
3.72
3.68
3.68
3.67
3.70
3.68
3.78
4.31
4.34
5.03
4.68
4.65
122
4.44
4.19
4.66
4.66
4.66
4.65
4.64
4.61
4.62
4.63
4.63
4.64
4.65
4.67
4.63
4.63
4.69
4.71
4.76
4.79
4.85
4.89
4.92
4.96
5.00
5.03
5.10
5.14
5.18
5.23
5.28
5.34
5.40
5.46
8.40
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.44
8.34
8.30
8.37
8.43
8.42
8.44
8.45
8.45
8.46
8.46
8.47
8.48
8.48
8.48
8.48
8.45
8.48
8.48
8.47
8.49
12/14/2013 6:14
12/14/2013 6:24
12/14/2013 6:34
12/14/2013 6:44
12/14/2013 6:54
12/14/2013 7:04
12/14/2013 7:14
12/14/2013 7:24
12/14/2013 7:34
12/14/2013 7:44
12/14/2013 7:54
12/14/2013 8:04
12/14/2013 8:14
12/14/2013 8:24
12/14/2013 8:34
12/14/2013 8:44
12/14/2013 8:54
12/14/2013 9:04
12/14/2013 9:14
12/14/2013 9:24
12/14/2013 9:34
12/14/2013 9:44
12/14/2013 9:54
12/14/2013 10:04
12/14/2013 10:14
12/14/2013 10:24
12/14/2013 10:34
12/14/2013 10:44
12/14/2013 10:54
12/14/2013 11:04
12/14/2013 11:14
12/14/2013 11:24
12/14/2013 11:34
12/14/2013 11:44
3.25
3.12
2.89
3.04
2.79
2.91
3.04
3.04
3.13
3.13
3.04
2.96
3.05
3.13
2.90
3.36
3.01
3.04
3.26
3.08
3.11
3.16
3.03
3.14
3.24
3.21
3.13
3.29
3.28
3.32
3.33
3.36
3.40
3.96
4.18
4.17
4.15
4.17
4.18
4.19
4.18
4.17
4.17
4.17
4.17
4.17
4.18
4.19
4.22
4.23
4.27
4.30
4.31
4.36
4.41
4.45
4.51
4.53
4.57
4.60
4.62
4.65
4.69
4.77
4.88
4.95
4.93
4.95
7.88
8.20
8.19
8.24
8.22
8.18
8.22
8.21
8.22
8.19
8.20
7.99
8.00
7.93
7.99
8.06
8.12
8.13
8.21
8.16
8.13
8.23
8.33
8.26
8.21
8.25
8.23
8.19
8.23
8.21
8.20
8.10
8.06
8.19
10/22/13 13:33
10/22/13 13:43
10/22/13 13:53
10/22/13 14:03
10/22/13 14:13
10/22/13 14:23
10/22/13 14:33
10/22/13 14:43
10/22/13 14:53
10/22/13 15:03
10/22/13 15:13
10/22/13 15:23
10/22/13 15:33
10/22/13 15:43
10/22/13 15:53
10/22/13 16:03
10/22/13 16:13
10/22/13 16:23
10/22/13 16:33
10/22/13 16:43
10/22/13 16:53
10/22/13 17:03
10/22/13 17:13
10/22/13 17:23
10/22/13 17:33
10/22/13 17:43
10/22/13 17:53
10/22/13 18:03
10/22/13 18:13
10/22/13 18:23
10/22/13 18:33
10/22/13 18:43
10/22/13 18:53
10/22/13 19:03
5.76
5.85
5.94
6.02
6.09
6.16
6.27
6.36
6.44
6.45
6.51
6.52
6.53
6.55
6.56
6.57
6.58
6.58
6.61
6.60
6.60
6.60
6.60
6.61
6.62
6.62
6.58
6.54
6.52
6.48
6.41
6.37
6.31
6.26
6.41
6.49
6.59
6.69
6.79
6.86
6.95
7.04
7.10
7.15
7.20
7.23
7.24
7.26
7.27
7.29
7.30
7.30
7.31
7.31
7.31
7.31
7.31
7.31
7.30
7.30
7.27
7.24
7.19
7.16
7.11
7.05
7.01
6.95
8.37
8.37
8.38
8.40
8.41
8.41
8.43
8.44
8.44
8.45
8.46
8.47
8.49
8.49
8.50
8.51
8.51
8.52
8.52
8.53
8.53
8.54
8.55
8.56
8.55
8.54
8.54
8.53
8.53
8.53
8.53
8.54
8.53
8.52
11/9/2013 11:46
11/9/2013 11:56
11/9/2013 12:06
11/9/2013 12:16
11/9/2013 12:26
11/9/2013 12:36
11/9/2013 12:46
11/9/2013 12:56
11/9/2013 13:06
11/9/2013 18:29
11/9/2013 18:39
11/9/2013 18:49
11/9/2013 18:59
11/9/2013 19:09
11/9/2013 19:19
11/9/2013 19:29
11/9/2013 19:39
11/9/2013 19:49
11/9/2013 19:59
11/9/2013 20:09
11/9/2013 20:19
11/9/2013 20:29
11/9/2013 20:39
11/9/2013 20:49
11/9/2013 20:59
11/9/2013 21:09
11/9/2013 21:19
11/9/2013 21:29
11/9/2013 21:39
11/9/2013 21:49
11/9/2013 21:59
11/9/2013 22:09
11/9/2013 22:19
11/9/2013 22:29
5.90
5.47
4.31
4.76
4.37
4.43
4.41
4.48
4.56
7.98
5.94
5.67
5.54
5.31
5.30
5.23
5.16
5.07
4.97
4.91
4.81
4.74
4.66
4.59
4.53
4.49
4.45
4.03
4.22
4.22
4.17
3.96
3.93
3.97
123
5.51
5.57
5.62
5.68
5.76
5.82
5.88
5.94
5.99
6.40
6.28
6.28
6.15
6.09
6.02
5.95
5.89
5.84
5.78
5.75
5.69
5.63
5.60
5.56
5.52
5.48
5.44
5.05
5.24
5.22
5.19
5.09
5.11
5.08
8.49
8.49
8.47
8.45
8.48
8.50
8.50
8.51
8.51
8.57
8.42
8.52
8.53
8.52
8.54
8.54
8.52
8.52
8.50
8.50
8.48
8.48
8.47
8.46
8.46
8.45
8.44
8.44
8.44
8.45
8.44
8.44
8.43
8.43
12/14/2013 11:54
12/14/2013 12:04
12/14/2013 12:14
12/14/2013 12:24
12/14/2013 12:34
12/14/2013 12:44
12/14/2013 12:54
12/14/2013 13:04
12/14/2013 13:14
12/14/2013 13:24
12/14/2013 13:34
12/14/2013 13:44
12/14/2013 13:54
12/14/2013 14:04
12/14/2013 14:14
12/14/2013 14:24
12/14/2013 14:34
12/14/2013 14:44
12/14/2013 14:54
12/14/2013 15:04
12/14/2013 15:14
12/14/2013 15:24
12/14/2013 15:34
12/14/2013 15:44
12/14/2013 15:54
12/14/2013 16:04
12/14/2013 16:14
12/14/2013 16:24
12/14/2013 16:34
12/14/2013 16:44
12/14/2013 16:54
12/14/2013 17:04
12/14/2013 17:14
12/14/2013 17:24
3.51
3.55
3.49
3.65
3.77
3.67
3.77
3.70
3.71
3.81
3.88
3.89
3.84
4.01
3.97
4.13
3.94
3.97
3.93
4.00
3.80
3.99
3.96
4.08
3.99
3.92
4.01
4.02
3.84
3.90
4.00
3.94
3.90
3.91
4.91
5.00
5.22
5.29
5.37
5.34
5.28
5.34
5.41
5.54
5.58
5.61
5.61
5.71
5.56
5.67
5.77
5.70
5.51
5.57
5.65
5.67
5.61
5.60
5.51
5.52
5.50
5.47
5.43
5.41
5.40
5.33
5.31
5.27
8.12
8.13
8.22
8.49
8.39
8.45
8.40
8.40
8.39
8.39
8.41
8.35
8.32
8.35
8.32
8.38
8.43
8.42
8.36
8.38
8.33
8.38
8.41
8.41
8.35
8.38
8.36
8.40
8.40
8.41
8.36
8.37
8.36
8.37
10/22/13 19:13
10/22/13 19:23
10/22/13 19:33
10/22/13 19:43
10/22/13 19:53
10/22/13 20:03
10/22/13 20:13
10/22/13 20:23
10/22/13 20:33
10/22/13 20:43
10/22/13 20:53
10/22/13 21:03
10/22/13 21:13
10/22/13 21:23
10/22/13 21:33
10/22/13 21:43
10/22/13 21:53
10/22/13 22:03
10/22/13 22:13
10/22/13 22:23
10/22/13 22:33
10/22/13 22:43
10/22/13 22:53
10/22/13 23:03
10/22/13 23:13
10/22/13 23:23
10/22/13 23:33
10/22/13 23:43
10/22/13 23:53
10/23/13 0:03
10/23/13 0:13
10/23/13 0:23
10/23/13 0:33
10/23/13 0:43
6.21
6.09
6.01
5.93
5.85
5.88
5.82
5.76
5.71
5.66
5.61
5.56
5.51
5.47
5.43
5.38
5.33
5.30
5.26
5.22
5.18
5.13
5.10
5.06
5.17
5.12
5.07
5.03
4.96
4.91
4.86
4.82
4.80
4.75
6.89
6.83
6.75
6.70
6.63
6.56
6.51
6.46
6.41
6.36
6.30
6.24
6.19
6.14
6.09
6.03
5.97
5.93
5.89
5.84
5.80
5.75
5.71
5.66
5.62
5.58
5.55
5.51
5.48
5.45
5.42
5.37
5.34
5.31
8.53
8.52
8.53
8.50
8.49
8.48
8.47
8.46
8.45
8.44
8.43
8.41
8.40
8.39
8.38
8.36
8.35
8.34
8.33
8.32
8.32
8.31
8.31
8.30
8.30
8.29
8.29
8.28
8.27
8.26
8.26
8.25
8.24
8.24
11/9/2013 22:39
11/9/2013 22:49
11/9/2013 22:59
11/9/2013 23:09
11/9/2013 23:19
11/9/2013 23:29
11/9/2013 23:39
11/9/2013 23:49
11/9/2013 23:59
11/10/2013 0:09
11/10/2013 0:19
11/10/2013 0:29
11/10/2013 0:39
11/10/2013 0:49
11/10/2013 0:59
11/10/2013 1:09
11/10/2013 1:19
11/10/2013 1:29
11/10/2013 1:39
11/10/2013 1:49
11/10/2013 1:59
11/10/2013 2:09
11/10/2013 2:19
11/10/2013 2:29
11/10/2013 2:39
11/10/2013 2:49
11/10/2013 2:59
11/10/2013 3:09
11/10/2013 3:19
11/10/2013 3:29
11/10/2013 3:39
11/10/2013 3:49
11/10/2013 3:59
11/10/2013 4:09
3.82
3.81
3.79
3.98
3.76
3.69
3.75
3.53
3.59
3.57
4.10
3.95
3.90
3.77
3.74
3.76
3.71
3.70
3.67
3.66
3.66
3.64
3.63
3.63
3.61
3.60
3.58
3.57
3.56
3.54
3.55
3.50
3.51
3.52
124
5.06
4.97
4.95
4.93
4.90
4.84
4.86
4.81
4.76
4.77
4.95
4.92
4.89
4.78
4.68
4.76
4.71
4.66
4.76
4.69
4.65
4.69
4.67
4.67
4.66
4.68
4.65
4.72
4.71
4.70
4.70
4.69
4.68
4.68
8.43
8.43
8.42
8.42
8.42
8.42
8.41
8.41
8.41
8.41
8.40
8.38
8.38
8.37
8.36
8.36
8.36
8.35
8.35
8.34
8.34
8.35
8.34
8.34
8.34
8.35
8.35
8.35
8.36
8.38
8.38
8.38
8.38
8.38
12/14/2013 17:34
12/14/2013 17:44
12/14/2013 17:54
12/14/2013 18:04
12/14/2013 18:14
12/14/2013 18:24
12/14/2013 18:34
12/14/2013 18:44
12/14/2013 18:54
12/14/2013 19:04
12/14/2013 19:14
12/14/2013 19:24
12/14/2013 19:34
12/14/2013 19:44
12/14/2013 19:54
12/14/2013 20:04
12/14/2013 20:14
12/14/2013 20:24
12/14/2013 20:34
12/14/2013 20:44
12/14/2013 20:54
12/14/2013 21:04
12/14/2013 21:14
12/14/2013 21:24
12/14/2013 21:34
12/14/2013 21:44
12/14/2013 21:54
12/14/2013 22:04
12/14/2013 22:14
12/14/2013 22:24
12/14/2013 22:34
12/14/2013 22:44
12/14/2013 22:54
12/14/2013 23:04
3.70
3.70
3.68
3.69
3.69
3.61
3.59
3.56
3.52
3.53
3.45
3.44
3.41
3.37
3.33
3.32
3.27
3.24
3.23
3.20
3.16
3.14
3.10
3.07
3.04
3.02
3.00
2.99
2.96
2.95
2.92
2.90
2.90
2.88
5.24
5.21
5.18
5.15
5.13
5.10
5.07
5.02
5.00
4.96
4.93
4.89
4.86
4.83
4.80
4.77
4.74
4.70
4.68
4.65
4.62
4.60
4.57
4.55
4.53
4.51
4.48
4.47
4.46
4.43
4.41
4.37
4.36
4.36
8.30
8.34
8.37
8.47
8.50
8.54
8.54
8.53
8.56
8.53
8.53
8.53
8.51
8.50
8.48
8.46
8.44
8.43
8.42
8.40
8.39
8.37
8.36
8.33
8.34
8.33
8.32
8.31
8.33
8.34
8.33
8.31
8.34
8.33
10/23/13 0:53
10/23/13 1:03
10/23/13 1:13
10/23/13 1:23
10/23/13 1:33
10/23/13 1:43
10/23/13 1:53
10/23/13 2:03
10/23/13 2:13
10/23/13 2:23
10/23/13 2:33
10/23/13 2:43
10/23/13 2:53
10/23/13 3:03
10/23/13 3:13
10/23/13 3:23
4.72
4.69
4.66
4.64
4.62
4.58
4.56
4.58
4.49
4.44
4.41
4.38
4.36
4.33
4.30
4.30
5.27
5.24
5.21
5.19
5.16
5.13
5.10
5.06
5.02
4.99
4.95
4.92
4.89
4.87
4.83
4.81
8.23
8.23
8.22
8.20
8.20
8.19
8.19
8.19
8.19
8.20
8.19
8.18
8.19
8.20
8.18
8.19
11/10/2013 4:19
11/10/2013 4:29
11/10/2013 4:39
11/10/2013 4:49
11/10/2013 4:59
11/10/2013 5:09
11/10/2013 5:19
11/10/2013 5:29
11/10/2013 5:39
11/10/2013 5:49
11/10/2013 5:59
11/10/2013 6:09
11/10/2013 6:19
11/10/2013 6:29
11/10/2013 6:39
11/10/2013 6:49
3.50
3.48
3.49
3.46
3.49
3.52
3.25
3.30
3.25
3.28
3.21
3.20
3.24
3.27
3.22
3.40
125
4.68
4.67
4.67
4.66
4.65
4.65
4.63
4.64
4.63
4.62
4.62
4.61
4.60
4.60
4.59
4.59
8.30
8.29
8.35
8.35
8.30
8.34
8.36
8.35
8.38
8.38
8.40
8.39
8.39
8.39
8.39
8.44
12/14/2013 23:14
12/14/2013 23:24
12/14/2013 23:34
12/14/2013 23:44
12/14/2013 23:54
12/15/2013 0:04
12/15/2013 0:14
12/15/2013 0:24
12/15/2013 0:34
12/15/2013 0:44
12/15/2013 0:54
12/15/2013 1:04
12/15/2013 1:14
12/15/2013 1:24
12/15/2013 1:34
12/15/2013 1:44
2.88
2.87
2.85
2.84
2.84
2.82
2.80
2.79
2.79
2.80
2.79
2.77
2.78
2.77
2.76
2.76
4.34
4.32
4.31
4.30
4.29
4.29
4.27
4.26
4.26
4.25
4.25
4.24
4.24
4.23
4.22
4.23
8.33
8.34
8.34
8.32
8.33
8.31
8.27
8.23
8.26
8.24
8.23
8.21
8.19
8.21
8.20
8.21
Appendix 3.
Date
Jan
Jan
Jan
Jan
Jan
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Feb
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Mar
Apr
May
May
May
May
Jun
Jun
Jun
Jun
Jul
Algae Dry Mass
(g)
0.1851
0.1563
0.1825
0.1481
0.1711
0.2865
0.1167
0.0968
0.4371
0.2654
0.1758
0.303
0.3412
0.1945
0.1677
0.1093
0.1315
0.1167
0.1709
0.158
0.2843
0.058
0.4075
0.5052
0.3781
0.4242
0.6822
0.4225
0.6113
0.4657
0.6931
0.2703
0.4542
0.5557
0.2101
0.2627
0.1786
0.281
0.2452
0.3167
0.1022
0.0398
0.0559
0.0116
0.1456
126
Jul
Jul
Jul
Aug
Aug
Aug
Aug
Aug
Sep
Sep
Sep
Sep
Sep
Oct
Oct
Oct
Oct
Oct
Nov
Nov
Nov
Nov
Nov
Dec
Dec
Dec
Dec
Dec
0.0851
0.1456
0.2073
0.0308
0.022
0.1392
0.2164
0.0599
0.2026
0.1339
0.1778
0.1317
0.298
0.0808
0.1459
0.2594
0.2875
0.2747
0.0955
0.2532
0.2176
0.2566
0.0464
0.4601
0.3015
0.2218
0.4556
0.3306
127
Appendix 4.
Time
0:00
6:00
9:00
12:00
15:00
18:00
0:00
6:00
9:00
12:00
15:00
18:00
0:00
6:00
9:00
12:00
15:00
18:00
January
2.256164
2.107671
2.121442
3.20
3.49
3.53
3.38
3.05
3.37
3.01
2.94
2.91
2.76
3.12
3.069841
2.356985
2.380766
February
3.10
3.55
3.28
3.30
2.94
3.14
3.52
3.44
3.29
3.27
3.24
3.44
3.63
3.40
3.25
3.52
3.19
3.38
March April
5.39 18.54
5.16 5.60
4.76 5.10
4.65 5.77
4.47 5.16
4.84 5.11
3.55 4.85
3.49 4.60
3.38 4.41
3.28 4.38
3.48 4.23
3.52 4.09
3.85
3.35
3.34
3.43
3.45
3.67
May
Water Column TOC, mg L-1
June July
August September
4.14
3.97 13.60
3.47 8.15
3.06 13.28
8.65 8.52
4.00 22.29
10.12
3.48 16.44
3.36 7.26
3.81 4.95
3.05 4.71
4.48 4.08
3.88 4.35
3.89 4.16
3.68 4.05
3.94 4.11
3.7465 4.09
17.09
4.16
3.69
3.96
3.79
4.30
3.84
3.78
3.66
3.70
3.71
3.78
3.63
3.67
3.53
3.48
3.42
3.49
128
4.14
3.73
4.07
4.52
3.65
3.48
3.29
3.45
3.35
3.92
3.64
4.08
4.14
3.97
3.45
3.81
7.31
8.20
5.28
10.03
5.69
3.64
4.46
4.50
5.03
3.86
4.25
3.96
4.58
4.40
4.29
5.19
4.53
3.59
October
3.80
3.64
3.26
3.06
3.80
3.59
3.61
3.74
3.82
3.29
3.61
3.45
3.90
4.26
4.19
4.17
November
6.77
4.99
4.91
4.91
4.51
4.76
4.54
5.27
4.65
4.49
4.55
4.21
4.04
4.18
4.72
4.11
4.21
7.34
December
4.27
3.74
3.42
3.29
4.51
4.15
4.11
3.58
3.29
3.25
4.029
3.65
3.393
3.085
3.1525
3.044
3.19
3.4595
Appendix 5.
Time
January
February March April May
0:00 6.059749
5.06
6.73 13.90
7.367
6:00
4.82673
5.07
7.94 10.03 4.7365
9:00 3.255742
6.46
7.37 7.32 5.8575
12:00
5.10
4.10 10.30 7.78
7.59
15:00
5.62
6.12
8.67 9.14
6.717
18:00
5.03
5.48
8.75 6.18
0:00
4.59
4.55
5.78 6.36
4.858
6:00
5.33
6.94 5.27
9:00
4.11
6.04
5.13 6.16
4.19
12:00
4.06
6.20
5.26 5.90 4.0675
15:00
3.24
6.50
6.37 4.87 4.2125
18:00
6.38
6.12
5.92 4.19
4.30
0:00
3.09
5.97
8.02
4.7765
6:00
5.15 13.52
4.49
9:00
5.22
6.25
3.58
12:00 10.34631
9.36
7.10
4.592
15:00 4.702073
5.60
7.39
5.1475
18:00 6.339647
4.96
4.44
4.5845
Algal Biofilm TOC, mg L-1
June July August September October November
December
5.60
5.27
8.10
5.36
8.39
5.46
4.37
3.74
5.15
5.67
5.17
10.86 4.42
4.22
4.71
5.80
4.79
10.03 4.29
4.85
6.33
4.82
5.59
4.44
8.15 4.60
3.94
4.97
4.73
5.32
13.45
8.83 4.31
3.85
4.65
4.28
9.46
6.09
5.15 3.95
5.10
4.99
3.53
5.32
4.35
4.56 3.97
5.53
4.71
5.35
3.91
4.41 3.86
3.83
4.52
3.99
5.20
3.63
4.80 4.34
4.79
4.47
4.15
5.19
3.84
5.04 3.91
4.20
4.25
4.62
5.39
4.9995
4.72 4.13
4.29
4.85
5.34
5.63
4.42
2.30 4.02
4.68
5.94
4.63
5.18
3.912
4.44 4.17
5.62
4.37
4.33
3.9185
4.34 4.14
4.24
4.13
5.25
4.82
4.016
4.38 4.16
3.87
5.12
4.52
4.80
4.5295
4.54 4.47
3.62
4.52
4.72
4.16
4.30
4.41 4.01
3.81
4.12
4.70
3.07
4.202
129
Appendix 6.
Diurnal water column nitrate concentrations, mg L-1
January
February
March
WC 0600
14.100 WC 0000
31.528
25.844
36.222 WC 0000
88.978
87.531
104.620
108.071
WC 0600
14.396 WC 0000
33.984
25.719
37.543 WC 0000
94.397
88.159
102.500
98.007
WC 0600
14.629 WC 0000
33.975
24.708
36.986 WC 0000
92.395
91.922
107.148
106.560
WC 0600
14.082 WC 0000
34.083
25.782
38.308 WC 0000
90.183
86.680
98.809
101.841
WC 0600
14.798 WC 0000
33.355
25.273
38.281 WC 0600
93.622
85.719
95.424
95.586
WC 0600
14.736 WC 0000
34.187
25.636
38.506 WC 0600
88.501
87.216
94.224
94.573
WC 0900
13.987 WC 0600
39.394
27.420
39.295 WC 0600
88.996
82.505
99.578
94.735
WC 0900
13.949 WC 0600
39.285
27.568
38.561 WC 0600
90.332
86.278
108.044
95.214
WC 0900
13.835 WC 0600
35.341
26.683
38.262 WC 0900
96.501
92.899
110.992
93.527
WC 0900
13.532 WC 0600
38.793
27.954
38.808 WC 0900
90.323
84.101
84.697
93.945
WC 0900
14.327 WC 0600
34.111
27.242
39.145 WC 0900
86.946
73.039
97.092
85.318
WC 0900
13.377 WC 0600
32.162
27.286
40.041 WC 0900
85.455
83.867
99.902
89.230
WC 1200
10.721
14.237 WC 0900
34.118
26.491
37.005 WC 1200
89.592
84.902
86.315
88.346
WC 1200
10.630
14.678 WC 0900
34.511
27.940
36.232 WC 1200
91.682
85.492
88.103
84.701
WC 1200
9.207
14.889 WC 0900
35.762
26.488
37.938 WC 1200
78.952
85.553
90.260
84.795
WC 1200
9.829
14.140 WC 0900
36.290
27.843
38.916 WC 1200
79.528
87.051
88.243
85.124
WC 1200
10.207
14.246 WC 0900
36.701
18.919
38.712 WC 1500
93.014
78.997
96.606
82.539
WC 1200
10.383
13.854 WC 0900
39.240
20.485
37.217 WC 1500
87.478
75.046
94.517
91.533
WC 1500
11.087
14.713 WC 1200
30.200
27.598
39.902 WC 1500
89.031
82.673
85.230
82.770
WC 1500
10.771
15.484 WC 1200
30.633
27.941
37.078 WC 1500
87.621
86.408
87.872
84.935
WC 1500
10.544
14.560 WC 1200
30.859
27.816
32.166 WC 1800
91.135
84.520
91.698
81.151
130
WC 1500
11.225
14.473 WC 1200
30.666
27.727
32.190 WC 1800
86.942
83.380
97.267
83.206
WC 1500
10.835
14.042 WC 1200
29.155
27.954
38.649 WC 1800
91.488
83.856
101.004
82.991
WC 1500
11.088
15.153 WC 1200
29.707
27.446
38.097 WC 1800
90.517
83.232
98.585
81.309
WC 1800
11.571
15.143 WC 1500
25.764
30.550
35.563
WC 1800
11.451
14.851 WC 1500
26.905
31.577
37.033
WC 1800
11.376
14.709 WC 1500
23.984
30.869
37.038
WC 1800
11.343
14.650 WC 1500
24.655
29.778
37.988
WC 1800
11.284
14.880 WC 1500
25.838
29.748
37.614
WC 1800
11.718
15.356 WC 1500
25.566
31.144
38.372
WC 0000
12.336
14.955 WC 1800
24.005
32.278
37.191
WC 0000
12.104
15.003 WC 1800
24.913
32.044
39.919
WC 0000
11.436
14.000 WC 1800
22.980
31.380
38.809
WC 0000
11.885
15.269 WC 1800
24.638
32.325
35.386
WC 0000
11.836
14.679 WC 1800
24.966
33.080
35.663
WC 0000
12.308
14.899 WC 1800
24.853
32.909
Appendix 6. (continued)
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0000
0000
0600
0600
April
16.613
14.164
19.261
20.195
18.182
18.212
20.473
20.443
May
23.941
25.001
28.248
28.979
30.57
30.681
30.696
31.29
WC
WC
WC
WC
WC
WC
WC
WC
_0000
_0000
_0000
_0000
_0000
_0000
_0000
_0000
13.418
12.472
13.226
13.428
12.85
12.915
13.278
13.786
29.738
30.886
29.101
28.513
32.648
32.787
31.558
31.229
131
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0600
0600
0600
0600
June
92.51
88.819
89.928
90.666
86.825
88.644
68.591
80.391
79.129
74.909
82.67
73.632
72.888
74.531
73.065
68.734
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
15.483
15.91
18.643
17.25
19.754
20.13
12.811
11.691
18.535
18.046
17.591
17.834
15.234
15.467
17.889
19.995
16.434
16.93
19.83
18.72
21.276
20.078
19.036
19.669
19.81
19.389
18.265
18.735
27.564
28.352
31.321
31.387
20.703
23.004
16.497
20.362
27.748
27.614
23.453
24.532
21.966
24.238
26.69
26.006
26.008
23.712
23.236
22.475
26.47
26.835
24.665
24.225
24.591
24.229
24.322
23.688
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
_0600
_0600
_0600
_0600
_0600
_0600
_0600
_0600
_0900
_0900
_0900
_0900
_0900
_0900
_0900
_0900
_1200
_1200
_1200
_1200
_1200
_1200
_1200
_1200
_1500
_1500
_1500
_1500
_1500
_1500
_1500
15.046
14.502
17.011
17.905
16.698
16.905
16.749
16.793
17.053
17.522
14.472
14.43
16.75
17.288
16.711
17.497
10.827
10.627
11.346
11.671
8.357
8.706
10.876
10.536
11.938
12.204
12.176
12.278
11.655
12.457
11.775
18.281
19.285
19.668
19.213
19.157
19.159
20.415
20.316
17.587
18.883
19.008
18.915
18.909
18.327
18.228
19.19
132
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
93.036
86.068
67.756
74.488
79.104
82.162
76.541
75.794
82.062
78.686
75.993
75.432
78.404
78.312
76.718
73.48
75.815
72.939
60.548
74.358
70.409
71.878
67.477
68.074
70.112
68.766
69.139
68.771
69.064
70.889
67.923
68.506
WC
WC
WC
WC
WC
WC
WC
WC
WC
_1500
_1800
_1800
_1800
_1800
_1800
_1800
_1800
_1800
11.717
28.075
28.3
29.063
28.217
28.407
28.238
29.266
30.332
20.793
20.069
20.281
21.73
19.712
20.784
20.831
20.472
Appendix 6. (continued)
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
July
64.046
65.402
62.416
65.459
72.979
73.773
75.206
70.835
75.958
72.542
70.22
72.586
79.776
77.726
76.807
76.018
74.84
50.977
53.892
53.143
55.78
61.235
62.769
60.762
65.342
81.15
64.914
83.636
77.652
74.555
74.846
77.261
73.152
59.405
46.181
49.002
45.841
46.208
53.568
58.058
50.055
58.711
61.194
60.627
61.748
60.97
39.573
43.129
44.617
51.208
56.285
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
August
33.244
27.963
31.103
31.477
31.908
31.805
26.722
27.394
31.376
30.587
31.637
34.118
29.492
28.733
28.23
29.259
31.124
27.195
26.724
25.953
25.983
24.602
26.323
24.049
23.626
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
33.902 WC
133
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
September
7.877
3.21
7.946
3.92
3.259 14.419
2.968 13.611
6.065
2.101
6.149
1.262
6.918
1
6.949
1.174
10.555 12.203
10.485 13.346
11.665 13.391
11.467 12.275
7.582
2.219
7.446
1.138
7.75
12.29
8.012 12.343
11.483
17.49
3.353
3.67
8.48
8.538
8.31
8.588
9.871
9.659
6.937
7.089
5.769
5.738
6.575
6.293
6.49
6.476
5.382
WC
WC
WC
WC
WC
WC
WC
1500
1500
1500
1800
1800
1800
1800
69.941
73.502
72.906
70.114
67.684
66.109
65.897
60.227
69.389
70.104
53.28
56.484
59.581
58.425
53.959
44.894
48.886
40.848
45.436
38.323
44.324
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
29.347
30.598
30.378
28.428
27.96
26.977
28.203
29.874
28.213
26.68
27.891
28.275
28.308
25.079
26.494
27.115
29.536
28.966
30.292
28.274
32.185
29.71
29.214
28.396
30.408
27.222
28.909
30.312
30.621
26.97
28.875
29.954
29.945
30.617
27.926
30.173
31.849
32.241
42.853
45.421
43.931
46.328
44.368
43.575
41.718
43.589
43.051
42.474
37.418
33.404
36.807
36.342
33.145
31.747
35.275
34.945
39.131
39.243
38.633
37.795
35.351
32.592
134
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
10.775
6.387
6.485
7.081
6.801
8.816
8.177
8.957
8.935
12.134
11.692
8.712
8.122
7.99
8.632
8.295
8.663
6.355
6.336
7.338
7.314
6.673
6.495
9.835
10.208
10.563
10.427
9.718
7.187
3.432
4.005
16.29
15.075
14.458
12.966
13.11
22.761
23.938
5.08
6.272
7.254
8.892
17.971
18.741
23.445
23.966
23.162
24.479
14.686
14.238
21.89
22.93
22.438
21.983
0.855
0.875
19.239
17.001
21.141
23.318
13.746
13.483
5.052
1.11
1.172
5.14
5.179
5.316
5.327
5.661
4.109
5.553
5.733
5.659
5.253
2.295
2.241
1.573
1.65
4.759
4.656
1.983
1.955
0.353
0.507
3.041
2.998
1.38
1.411
2.125
2.024
0.767
0.721
Appendix 6. (continued)
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
October
37.003
37.830
38.180
37.537
36.690
34.571
35.362
37.509
23.451
23.933
22.480
21.791
39.203
79.701
79.108
32.137
35.671
43.208
46.021
44.578
47.469
60.708
61.103
31.932
30.148
35.953
36.941
65.881
53.307
70.844
63.273
61.358
68.185
57.433
64.211
67.790
66.068
67.716
62.555
64.273
66.425
45.464
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
November
18.453
31.000
18.108
31.636
20.217
18.978
19.333
19.444
24.966
20.909
24.608
21.275
15.315
21.440
16.128
22.305
24.696
33.858
26.006
35.694
18.299
22.433
18.306
22.978
26.680
35.180
25.855
34.268
25.870
34.572
25.714
33.174
16.124
18.969
16.012
20.269
12.757
21.419
13.177
22.051
24.498
19.319
23.678
24.102
24.038
28.181
25.088
28.287
21.604
34.491
21.393
33.419
17.921
26.637
17.818
25.307
19.601
24.748
135
27.522
28.331
25.833
25.847
37.398
37.188
29.453
29.377
26.747
26.151
33.510
34.359
39.075
40.162
35.352
33.937
15.318
15.164
17.768
18.705
26.649
26.636
19.987
19.206
21.902
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
23.685 WC
24.373 WC
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
December
3.090
3.545
3.427
3.653
3.245
3.853
3.841
4.452
3.980
4.810
3.631
5.028
3.794
5.291
3.081
5.292
2.779
2.480
2.896
2.673
3.009
2.630
2.893
2.928
3.034
3.089
2.967
3.207
2.972
3.465
2.596
3.431
2.972
0.546
2.478
0.524
3.044
0.465
2.985
0.561
3.013
0.552
3.155
0.541
3.101
0.495
2.606
0.456
2.725
0.455
2.547
0.378
2.577
0.388
2.517
0.423
2.741
0.407
11.632
11.943
11.931
11.017
4.304
9.972
10.009
9.223
9.398
8.924
9.155
9.078
9.103
9.386
0.187
8.796
8.707
8.980
9.364
8.432
8.693
9.792
9.660
8.240
7.950
8.231
8.106
8.225
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
41.585
26.869
25.926
25.883
26.035
34.744
34.981
37.213
38.515
28.448
28.660
34.706
34.181
32.030
33.781
20.439
20.296
35.630
36.716
48.026
72.342
76.584
62.904
58.969
69.321
67.876
67.462
65.954
55.706
68.968
63.187
54.950
77.047
69.914
44.870
44.438
66.422
67.569
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
18.723
17.902
16.365
21.274
20.945
14.394
12.520
15.755
15.936
16.221
16.954
17.246
17.227
22.860
19.413
16.876
16.453
13.325
11.405
24.212
19.470
20.045
29.933
30.253
30.893
29.502
19.221
19.197
15.848
16.463
23.983
23.921
15.082
14.839
20.087
20.548
19.115
19.430
136
19.277
19.495
35.682
36.556
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
2.442
2.621
1.779
2.278
1.686
1.866
2.077
2.093
2.083
2.067
2.275
2.277
2.147
2.350
1.493
1.416
1.620
1.516
1.425
0.440
0.523
0.416
2.987
2.967
3.217
3.210
2.958
3.121
3.413
3.057
5.023
5.396
5.616
5.109
5.340
5.773
5.714
5.678
7.881
7.845
7.884
8.051
7.592
7.471
7.894
8.558
7.270
7.467
7.687
7.841
8.247
8.125
6.719
8.342
8.264
4.934
8.014
Appendix 7.
Diurnal algal biofilm nitrate concentrations, mg L-1
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
January
14.726
13.953
14.158
14.555
8.584
8.469
9.442
9.696
9.585
9.524
9.136
9.315
11.163
10.808
11.135
11.355
10.856
10.534
10.135
10.091
13.229
14.022
14.098
13.291
13.077
13.029
12.416
13.335
14.179
14.659
14.411
14.322
14.235
14.167
13.970
14.780
14.165
14.258
14.914
14.051
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
February
36.116
27.377
36.065
26.743
36.286
26.671
35.424
27.243
38.099
27.970
39.559
27.185
37.819
30.315
37.914
28.409
38.424
33.941
38.052
29.106
35.813
28.433
37.664
27.586
32.763
28.179
31.910
28.126
29.300
27.832
31.062
29.152
26.482
27.085
26.116
29.894
27.137
28.611
33.857
25.176
23.729
24.466
26.052
22.970
24.215
23.910
32.170
137
47.681
46.772
47.175
47.011
49.620
47.695
46.723
46.097
50.864
45.822
47.912
47.920
47.991
49.048
45.872
49.433
46.395
46.046
28.151
31.795
45.279
44.360
47.364
46.971
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
58.984
57.780
55.451
56.387
57.852
55.894
55.286
56.451
55.709
55.110
54.653
55.293
54.010
53.102
54.773
55.047
54.010
54.563
52.467
52.498
53.777
54.503
54.897
53.362
March
55.774
54.734
54.559
54.473
52.789
52.789
51.831
51.823
54.081
44.172
51.467
52.197
51.920
50.535
51.018
52.438
50.590
51.348
49.565
51.065
51.840
51.867
50.940
51.893
42.786
41.464
46.972
46.561
44.730
44.806
43.183
45.242
46.989
43.107
40.659
41.658
40.823
41.505
41.483
40.424
39.340
40.034
40.083
41.267
38.153
39.683
40.195
41.222
47.524
47.316
48.100
49.143
47.157
47.110
46.034
47.197
50.567
47.632
48.383
48.067
45.630
46.128
43.005
41.939
44.580
43.564
44.677
45.107
45.182
45.131
41.275
43.372
Appendix 7. (continued)
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
_0:00
_0:00
_0:00
_0:00
_0:00
_0:00
_0:00
_0:00
_6:00
_6:00
_6:00
_6:00
_6:00
_6:00
_6:00
_6:00
_9:00
_9:00
_9:00
_9:00
_9:00
_9:00
_9:00
_9:00
_12:00
_12:00
_12:00
_12:00
April
17.994
18.244
17.45
17.663
18.15
18.273
18.34
18.403
17.191
16.976
15.461
16.076
16.005
16.267
16.872
16.918
16.751
15.207
15.717
15.877
16.344
15.769
16.18
15.978
15.627
16.206
15.38
15.768
14.014
13.201
17.185
16.911
13.127
13.057
16.24
15.995
16.558
16.357
15.6
15.718
13.663
14.31
15.02
14.683
14.047
13.946
14.472
14.673
14.172
14.027
15.149
14.46
15.737
15.09
15.657
15.985
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
_0000
_0000
_0000
_0000
_0000
_0000
_0000
_0000
_6:00
_6:00
_6:00
_6:00
_6:00
_6:00
_6:00
_6:00
_9:00
_9:00
_9:00
_9:00
_9:00
_9:00
_9:00
_9:00
_1200
_1200
_1200
_1200
May
12.574
12.104
15.01
15.099
17.392
17.535
17.827
17.611
17.976
18.068
18.253
18.691
17.672
18.081
17.781
18.268
18.27
17.529
17.473
17.284
June
12.816
13.247
12.942
13.191
12.999
12.921
12.508
12.53
16.555
17.715
17.118
9.152
9.122
10.719
11.319
138
28.645
29.002
28.679
28.869
28.956
28.369
25.706
26.791
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
81.2
84.004
90.701
88.71
83.26
84.061
91.05
85.847
89.575
83.789
84.861
84.05
71.797
75.242
67.141
76.242
71.867
80.913
75.78
72.835
78.201
76.637
73.814
75.069
76.668
78.005
75.194
78.731
75.549
74.316
77.154
73.198
79.648
71.816
72.391
72.95
66.648
68.199
70.303
70.086
66.695
66.231
69.479
66.316
66.449
66.638
67.497
68.935
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
_12:00
_12:00
_12:00
_12:00
_15:00
_15:00
_15:00
_15:00
_15:00
_15:00
_15:00
_15:00
_18:00
_18:00
_18:00
_18:00
_18:00
_18:00
_18:00
_18:00
15.895
16.343
16.312
16.132
15.175
16.166
15.676
15.702
15.489
15.833
15.038
15.335
17.086
16.347
16.2
15.346
15.731
15.874
15.411
15.638
13.704
13.489
14.512
14.989
14.041
13.878
9.132
9.181
14.753
14.216
15.21
15.777
13.231
12.893
13.285
14.254
12.314
12.53
13.187
13.16
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
_1200
_1200
_1200
_1200
_1500
_1500
_1500
_1500
_1500
_1500
_1500
_1500
_1800
_1800
_1800
_1800
_1800
_1800
_1800
_1800
16.253
15.107
16.413
16.516
13.409
13.175
15.029
15.367
11.356
11.58
10.122
9.442
12.097
11.795
12.26
11.997
11.938
12.36
11.874
12.287
29.311
28.027
30.81
29.24
26.891
26.823
31.58
30.72
18.26
18.92
20.552
20.436
19.556
19.166
20.767
19.837
Appendix 7. (continued)
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
July
72.947
77.423
68.75
77.198
79.064
83.449
80.9
76.256
79.463
75.549
72.497
81.542
74.332
72.81
53.968
53.279
57.007
55.504
59.878
59.143
64.997
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0000
0000
0000
August
35.79
33.333
35.21
34.548
28.873
32.022
34.049
139
31.203
29.859
32.784
33.867
33.353
31.581
29.616
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0000
0000
0000
September
17.339
19.285
24.676
25.169
23.526
22.792
16.279
4.966
5.111
6.952
6.715
5.05
5.468
4.533
16.122
12.466
12.76
13.154
19.701
12.783
11.64
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
89.279
91.004
92.751
93.598
89.393
92.714
97.429
94.012
99.536
72.027
73.565
77.722
91.382
76.08
77.71
73.275
77.918
76.589
92.896
74.216
72.474
77.691
83.528
81.329
82.116
77.836
72.471
69.954
51.457
64.119
61.304
69.987
70.526
73.396
64.211
65.897
63.714
67.035
64.198
60.153
57.22
57.878
56.419
55.147
54.426
50.452
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
34.941
34
35.885
35.148
33.866
30.682
30.46
29.303
29.45
30.931
32.955
32.805
31.959
34.435
33.67
35.435
36.568
32.188
31.28
34.698
33.945
32.645
32.627
33.852
33.58
34.39
33.629
37.012
35.633
35.987
35.424
140
30.784 Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
37.178 Pore
35.139 Pore
37.055 Pore
37.041 Pore
31.963 Pore
32.8 Pore
36.539 Pore
38.262 Pore
53.742 Pore
55.163 Pore
53.334 Pore
54.794 Pore
54.738 Pore
48.502 Pore
49.632 Pore
48.984 Pore
39.947 Pore
41.537 Pore
33.555 Pore
33.271 Pore
44.59 Pore
44.025 Pore
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
12.436
23.075
21.152
22.802
23.374
24.783
23.068
20.789
21.88
15.476
13.993
22.886
23.663
21.637
20.894
23.055
21.984
23.331
24.032
20.758
21.489
20.901
21.589
21.624
22.102
23.558
24.552
25.196
24.633
25.158
25.515
4.673
9.001
8.852
8.535
8.454
8.617
8.431
6.822
6.877
10.85
10.824
9.894
9.71
5.803
5.592
5.672
5.734
12.865
12.815
12.676
12.062
13.81
13.35
14.488
13.889
7.007
6.708
11.227
11.705
13.831
13.657
12.182
9.527
9.426
9.281
9.751
9.038
8.578
9.304
7.906
7.182
8.82
14.056
8.939
6.24
14.426
6.492
6.482
6.649
11.095
12.13
11.348
6.454
6.519
5.889
5.858
4.47
4.541
5.036
6.229
4.898
5.088
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
35.602
35.604
34.126
34.256
33.391
33.344
34.517
35.952
33.394
33.197
40.884
40.188
49.033
49.683
47.253
48.533
48.851
49.156
47.614
47.983
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
25.568
25.58
17.092
16.649
22.618
22.154
24.38
23.079
24.847
24.827
12.686
12.001
7.833
8.32
8.907
8.766
9.823
10.041
4.856
4.216
2.686
2.772
3.245
3.429
3.787
3.899
3.786
3.582
5.278
4.777
Appendix 7. (continued)
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
October
35.406
34.559
34.289
34.929
34.587
34.158
33.938
33.618
46.844
43.518
44.144
45.292
40.314
36.645
39.946
36.033
44.118
46.457
41.747
40.277
48.521
43.211
43.699
44.376
41.785
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
November
24.803
31.699
25.956
30.723
26.130
30.012
25.500
32.651
26.871
30.503
26.922
29.783
25.059
23.252
28.033
24.758
25.754
25.425
25.887
26.150
24.384
31.781
24.088
32.212
19.875
27.070
20.255
27.813
25.894
32.852
24.652
33.168
24.296
33.527
141
34.014
34.499
37.764
37.889
34.341
34.172
34.278
35.007
23.295
23.746
40.409
40.651
33.739
32.602
33.347
34.277
29.237
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0600
0600
0900
December
2.228
8.012
2.202
8.061
2.142
8.305
1.996
8.418
1.783
6.617
1.695
7.321
1.564
7.590
1.121
7.761
1.967
5.226
1.929
5.355
1.962
8.676
1.606
5.629
1.307
6.051
1.309
5.702
1.956
6.038
1.312
5.778
1.350
8.776
8.641
8.139
9.720
10.645
10.706
11.642
12.514
11.147
12.102
10.148
9.942
10.598
10.794
10.172
9.862
8.818
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
37.433
39.029
32.236
33.375
37.288
39.231
38.835
39.376
30.965
33.685
23.247
24.013
32.405
31.702
33.486
31.760
26.426
26.228
27.429
26.382
35.015
35.779
35.892
35.571
40.374
28.111
28.436
49.332
44.971
44.881
46.713
46.955
44.705
39.235
38.454
45.692
42.358
43.498
42.809
43.558
45.152
46.540
43.293
45.286
48.885
47.228
44.443
35.479
34.457
39.870
39.058
38.252
42.273
43.848
40.605
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
25.332
27.975
26.851
27.164
25.632
23.040
22.112
25.781
25.493
21.396
20.863
23.338
22.267
23.756
24.891
24.151
23.974
24.888
25.436
14.173
13.879
23.835
23.124
22.728
22.524
15.859
15.810
24.677
24.272
22.137
22.214
32.996
26.981
27.840
28.052
28.987
31.546
33.827
23.531
23.961
26.216
26.435
25.515
25.673
25.421
26.461
21.755
22.194
28.767
27.853
28.633
29.151
24.368
23.251
23.998
24.987
22.774
23.056
18.824
18.580
23.188
23.035
142
29.812
30.708
29.385
39.863
39.424
29.892
29.439
24.660
22.990
35.364
33.865
22.691
24.242
29.635
26.943
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0900
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
1500
1500
1500
1500
1800
1800
1800
1800
1800
1800
1800
1800
1.317
1.257
1.430
1.479
1.104
1.112
1.118
1.104
1.184
1.593
1.336
1.141
1.339
1.196
1.209
1.472
1.620
1.474
1.459
1.501
1.248
1.134
0.960
0.673
0.611
0.567
0.568
0.507
0.518
0.637
0.967
2.261
2.517
2.475
2.804
2.955
2.822
2.611
0.200
0.163
0.216
0.207
0.190
0.185
0.162
0.161
3.408
3.468
3.569
3.523
4.075
3.756
3.892
0.006
5.912
5.954
5.977
6.352
6.271
5.337
6.297
6.277
9.256
9.429
9.786
9.759
9.829
9.472
9.013
8.773
8.206
8.905
9.540
9.247
8.978
8.489
8.290
9.527
9.105
8.951
9.214
9.026
9.201
8.978
9.251
10.080
9.856
7.781
8.255
4.635
8.640
8.268
7.394
Appendix 8.
Diurnal water column phosphate concentrations, mg L-1
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
January
1200
0.018
1200
0.000
1200
0.001
1200
0.000
1200
0.001
1500
0.003
1500
0.000
1500
0.000
1500
0.002
1500
0.000
1500
0.002
1800
0.006
1800
0.001
1800
0.000
1800
0.000
1800
0.000
1800
0.000
0000
0.000
0000
0.003
0000
0.000
0000
0.002
0000
0.000
0000
0.000
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
February
4.556 0.070
3.296 0.570
1.427 1.024
1.568 1.265
2.223 0.268
2.427 0.566
1.359 0.145
1.657 0.108
3.697 0.081
2.676 0.045
1.437 0.130
1.453 0.159
0.545 0.151
0.459 0.171
2.580 0.076
2.767 0.037
4.115 2.261
4.366 1.680
3.792 0.201
3.066 0.231
1.167 0.274
0.725 0.244
0.536 0.216
0.287 0.232
0.431 0.159
0.481 0.123
0.235 0.125
0.837 0.107
1.548
0.595
2.251
2.885
4.031
5.898
5.200
3.976
5.880
5.571
5.279
4.593
2.609
2.908
3.061
3.048
3.040
3.915
3.870
2.561
3.291
3.371
3.973
2.569
1.395
0.206
3.381
4.059
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
143
March
0.029 0.069
0.038 0.037
0.136 0.065
0.007 0.082
0.005 0.015
0.030 0.011
0.005 0.031
0.036 0.001
0.067 0.028
0.092 0.049
0.079 0.058
0.003 0.025
0.023 0.033
0.040 0.029
0.045 0.011
0.012 0.013
0.064 0.011
0.049 0.021
0.039 0.055
0.039 0.038
0.004 0.079
0.025 0.029
0.014 0.041
0.065 0.024
0.031
0.028
0.046
0.046
0.029
0.005
0.032
0.035
1.370
0.037
0.014
0.001
0.003
0.006
0.040
0.002
0.033
0.033
0.015
0.057
0.042
0.034
0.014
0.028
0.009
0.022
0.002
0.039
0.020
0.035
0.051
0.828
0.008
0.212
0.148
0.166
0.005
0.182
0.377
2.658
3.137
1.674
2.612
4.660
3.597
2.707
2.996
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
WC
0000
0000
0000
0000
0000
0000
0600
0600
0600
0600
0600
0600
0900
0900
0900
0900
0900
0900
1200
1200
1200
1200
1200
1200
1500
1500
1500
1500
April
0.090
0.053
0.011
0.051
0.006
0.062
0.034
0.035
0.025
0.030
0.014
0.022
0.042
0.044
0.037
0.069
0.011
0.016
0.061
0.066
0.041
0.048
0.017
0.006
0.053
0.048
0.055
0.045
0.031
0.015
0.015
0.046
0.017
0.018
0.037
0.023
0.009
0.007
0.013
0.015
0.013
0.013
0.063
0.042
0.022
0.018
0.012
0.015
0.007
0.009
0.014
0.003
0.017
0.016
0.016
0.019
WC
WC
WC
WC
WC
WC
WC
WC
1500
1500
1800
1800
1800
1800
1800
1800
0.261
0.296
0.251
0.093
0.430
0.520
0.256
0.269
0.616
1.174
0.853
0.628
0.404
0.222
1.494
2.764
3.491
2.540
3.008
3.067
3.945
2.856
2.140
WC
WC
WC
WC
WC
WC
WC
WC
1500
1500
1800
1800
1800
1800
1800
1800
0.030
0.021
0.031
0.032
0.048
0.029
0.023
0.022
0.014
0.020
0.004
0.001
0.008
0.026
0.034
0.006
Appendix 8. (continued)
May
WC _0000
WC _0000
WC _0000
WC _0000
WC _0000
WC _0000
WC _0000
WC _0000
WC _0600
WC _0600
WC _0600
WC _0600
WC _0600
WC _0600
WC _0600
WC _0600
WC _0900
WC _0900
WC _0900
10.222
9.045
10.602
12.724
13.993
11.598
9.772
10.047
13.626
11.762
11.686
10.052
9.277
9.924
12.411
11.903
16.448
14.297
9.921
18.989
19.700
13.637
13.336
41.194
46.605
37.055
36.554
10.338
9.530
8.261
WC 0000
WC 0000
WC 0000
WC 0000
WC 0600
WC 0600
WC 0600
WC 0600
WC 0900
WC 0900
WC 0900
WC 0900
WC 1200
WC 1200
WC 1200
WC 1200
WC 1500
WC 1500
WC 1500
June
0.136
0.301
0.313
0.459
0.294
0.396
0.010
0.223
7.756
1.840
1.282
1.563
1.534
1.454
2.161
1.875
3.202
3.213
2.765
79.129
74.909
82.670
73.632
72.888
74.531
73.065
68.734
75.815
72.939
60.548
74.358
70.409
71.878
67.477
68.074
70.112
68.766
69.139
WC 0000
WC 0000
WC 0000
WC 0000
WC 0600
WC 0600
WC 0600
WC 0600
WC 0900
WC 0900
WC 0900
WC 0900
WC 1200
WC 1200
WC 1200
WC 1200
WC 1500
WC 1500
WC 1500
144
July
27.928
29.849
30.380
28.533
34.636
31.247
35.813
33.098
31.797
32.534
30.068
30.059
35.117
33.594
34.299
34.583
27.836
30.399
30.844
26.756
31.450
39.094
41.347
46.243
42.860
42.036
49.597
32.680
44.885
24.101
24.502
41.588
38.893
33.447
34.697
39.326
37.281
33.006
24.433
29.326
25.862
26.524
24.808
24.594
20.825
16.235
23.727
25.963
22.154
19.948
24.808
29.084
28.452
27.265
29.523
28.264
29.830
August
WC 0000 6.689
WC 0000 5.753
WC 0000 6.972
WC 0000 8.851
WC 0000 8.248
WC 0000 9.936
WC 0000 3.164
WC 0000 6.979
WC 0600 4.876
WC 0600 6.203
WC 0600 7.516
WC 0600 9.753
WC 0600 9.426
WC 0600 6.647
WC 0600 6.274
WC 0600 6.112
WC 0900 6.056
WC 0900 6.094
WC 0900 6.684
7.334
4.135
2.818
3.179
4.109
5.431
1.256
1.474
5.424
4.540
7.354
6.783
5.554
7.669
6.215
5.502
5.355
5.736
3.582
WC _0900
WC _0900
WC _0900
WC _0900
WC _0900
WC _1200
WC _1200
WC _1200
WC _1200
WC _1200
WC _1200
WC _1200
WC _1200
WC _1500
WC _1500
WC _1500
WC _1500
WC _1500
WC _1500
WC _1500
WC _1500
WC _1800
WC _1800
WC _1800
WC _1800
WC _1800
WC _1800
WC _1800
WC _1800
10.577
9.924
12.039
16.079
15.022
7.489
7.418
7.118
8.381
5.750
5.473
7.617
6.774
5.796
5.841
5.580
5.620
5.755
5.521
4.165
5.547
11.802
5.188
8.163
8.069
4.284
7.019
8.465
11.163
8.830
7.444
7.106
14.111
15.211
45.494
36.347
22.683
42.369
40.603
22.400
29.627
26.080
WC 1500
WC 1800
WC 1800
WC 1800
WC 1800
2.765
7.061
6.713
4.793
5.187
68.771
69.064
70.889
67.923
68.506
WC 1500
WC 1800
WC 1800
WC 1800
WC 1800
19.392
19.462
20.001
17.063
15.015
14.412
13.842
13.203
145
27.606
27.999
30.496
24.552
24.739
27.360
29.584
31.426
34.249
35.702
28.032
13.028
17.544
14.675
12.266
WC 0900
WC 0900
WC 0900
WC 0900
WC 0900
WC 1200
WC 1200
WC 1200
WC 1200
WC 1200
WC 1200
WC 1200
WC 1200
WC 1500
WC 1500
WC 1500
WC 1500
WC 1500
WC 1500
WC 1500
WC 1500
WC 1800
WC 1800
WC 1800
WC 1800
WC 1800
WC 1800
WC 1800
WC 1800
6.352
5.966
6.452
7.859
7.348
7.054
7.764
6.189
6.629
5.411
6.616
0.928
2.537
3.256
5.297
6.961
7.577
3.979
4.437
4.691
4.844
6.829
7.012
5.400
5.559
8.101
7.689
4.737
5.743
5.294
4.232
3.152
4.923
5.863
7.348
8.441
6.309
6.939
6.911
6.040
8.750
6.565
6.562
4.694
5.886
5.379
5.359
6.913
5.422
4.152
Appendix 9.
Diurnal algal biofilm phosphate concentrations, mg L-1
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
January
1200 0.004
1200 0.008
1200 0.002
1200 0.000
1500 0.001
1500 0.000
1500 0.000
1500 0.000
1800 0.001
1800 0.000
1800 0.000
1800 0.000
0000 0.000
0000 0.002
0000 0.000
0000 0.007
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1800
1800
1800
1800
February
3.164 0.036
3.945 0.049
4.149 1.016
5.254 0.797
5.211 0.084
5.128 0.099
5.389 0.352
4.514 0.286
3.236 0.289
4.678 0.274
4.457 0.102
4.447 0.114
4.043 0.130
4.651 0.104
4.554 0.107
4.825 0.059
2.600 0.083
2.215 0.223
5.166 0.155
1.487 0.248
1.182 0.055
2.375 0.027
2.355 0.076
1.155
0.026
0.002
0.010
0.016
0.017
0.002
0.001
0.003
0.022
0.010
0.026
0.002
0.004
0.007
0.002
0.007
0.007
0.010
0.008
0.010
0.004
0.009
0.000
0.000
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
Pore
0000
0000
0000
0000
0600
0600
0600
0600
0900
0900
0900
0900
1200
1200
1200
1200
1500
1500
1500
1500
1800
1800
1800
1800
146
March
0.009 0.005
0.026 0.011
0.033 0.005
0.053 0.003
0.079 0.003
0.089 0.009
0.093 0.012
0.109 0.002
0.069 0.025
0.021 0.020
0.038 0.004
0.026 0.034
0.014 0.005
0.002 0.005
0.053 0.009
0.015 0.018
0.003 0.023
0.003 0.009
0.005 0.003
0.003 0.018
0.005 0.001
0.015 0.021
0.018 0.002
0.002 0.002
0.247
0.192
0.277
0.795
3.884
4.199
2.535
2.522
7.899
4.741
2.073
1.674
4.180
4.658
5.188
4.842
4.376
4.684
3.840
4.671
2.997
4.778
4.027
4.338
4.129
3.185
4.234
5.163
0.027
0.042
1.013
1.334
9.627
5.973
4.763
5.262
2.094
2.842
0.417
0.283
4.719
4.751
3.819
4.164
5.046
4.752
4.907
3.752
Pore _0:00
Pore _0:00
Pore _0:00
Pore _0:00
Pore _0:00
Pore _0:00
Pore _0:00
Pore _0:00
Pore _6:00
Pore _6:00
Pore _6:00
Pore _6:00
Pore _6:00
Pore _6:00
Pore _6:00
Pore _6:00
Pore _9:00
Pore _9:00
Pore _9:00
Pore _9:00
Pore _9:00
Pore _9:00
Pore _9:00
Pore _9:00
Pore _12:00
Pore _12:00
Pore _12:00
Pore _12:00
April
0.151
0.155
0.174
0.178
0.186
0.187
0.204
0.192
0.131
0.130
0.131
0.150
0.116
0.126
0.149
0.149
0.049
0.064
0.098
0.106
0.056
0.051
0.056
0.061
0.052
0.035
0.095
0.094
0.017
0.014
0.052
0.059
0.011
0.009
0.019
0.025
0.017
0.017
0.036
0.025
0.016
0.023
0.013
0.019
0.025
0.013
0.028
0.026
0.028
0.038
0.017
0.022
0.007
0.008
0.002
0.009
Pore _12:00
Pore _12:00
Pore _12:00
Pore _12:00
Pore _15:00
Pore _15:00
Pore _15:00
Pore _15:00
Pore _15:00
Pore _15:00
Pore _15:00
Pore _15:00
Pore _18:00
Pore _18:00
Pore _18:00
Pore _18:00
Pore _18:00
Pore _18:00
Pore _18:00
Pore _18:00
0.272
0.092
0.082
0.073
0.070
0.053
0.042
0.043
0.155
0.168
0.054
0.051
0.033
0.026
0.030
0.025
0.020
0.021
0.013
0.022
0.001
0.007
0.001
0.005
0.017
0.020
0.035
0.029
0.011
0.016
0.008
0.011
0.011
0.016
0.030
0.032
0.035
0.018
0.009
0.056
Appendix 9. (continued)
Pore _0000
Pore _0000
Pore _0000
Pore _0000
Pore _0000
Pore _0000
Pore _0000
May
7.799 7.542 22.490
8.248 6.583 19.353
13.270 9.182 23.400
10.856 7.834 23.864
13.794 9.469 22.717
15.731 10.620 19.331
12.920 7.606 13.479
Pore 0000
Pore 0000
Pore 0000
Pore 0000
Pore 0600
Pore 0600
Pore 0600
June
0.803
0.584
0.851
0.659
0.481
0.157
0.494
76.668
78.005
75.194
78.731
75.549
74.316
77.154
147
Pore 0000
Pore 0000
Pore 0000
Pore 0000
Pore 0600
Pore 0600
Pore 0600
July
28.190
27.420
31.632
24.870
32.811
34.299
31.311
29.193
30.420
34.024
39.562
33.753
43.664
46.753
26.944
24.723
21.953
25.780
22.691
22.201
22.136
Pore 0000
Pore 0000
Pore 0000
Pore 0000
Pore 0000
Pore 0000
Pore 0000
August
9.325
4.246
6.145
7.818
1.742
4.465
4.907
3.820
2.836
6.377
5.619
4.788
4.294
3.657
Pore _0000
Pore _0600
Pore _0600
Pore _0600
Pore _0600
Pore _0600
Pore _0600
Pore _0600
Pore _0600
Pore _0900
Pore _0900
Pore _0900
Pore _0900
Pore _0900
Pore _0900
Pore _0900
Pore _0900
Pore _1200
Pore _1200
Pore _1200
Pore _1200
Pore _1200
Pore _1200
Pore _1200
Pore _1200
Pore _1500
Pore _1500
Pore _1500
Pore _1500
Pore _1500
Pore _1500
14.699
17.576
16.720
15.744
18.682
13.827
11.693
16.471
16.868
15.864
15.184
13.087
15.565
1.906
15.145
12.103
12.519
8.666 11.163
Pore 0600
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1500
Pore 1500
Pore 1500
Pore 1500
Pore 1800
Pore 1800
Pore 1800
Pore 1800
0.456
2.262
0.518
0.653
0.513
0.267
0.238
0.260
0.238
0.200
1.242
1.153
0.182
3.854
4.279
3.550
2.244
73.198
79.648
71.816
72.391
72.950
66.648
68.199
70.303
70.086
66.695
66.231
69.479
66.316
66.449
66.638
67.497
68.935
5.915
5.378
9.663
7.526
6.689
7.610
5.998
5.864
5.522
6.750
6.096
4.886
7.545
6.881
148
Pore 0600
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1500
Pore 1500
Pore 1500
Pore 1500
Pore 1800
Pore 1800
Pore 1800
Pore 1800
27.939
25.262
31.032
25.082
25.195
31.476
26.815
24.182
18.431
34.526
34.998
32.904
30.743
30.637
31.273
25.139
21.108
44.526
34.269
34.232
41.225
44.508
34.827
34.612
39.398
35.635
34.311
34.570
29.693
33.972
31.666
32.572
33.234
31.290
18.920
23.595
18.346
21.771
20.940
22.801
23.769
22.947
19.931
20.077
20.247
13.925
Pore 0000
Pore 0600
Pore 0600
Pore 0600
Pore 0600
Pore 0600
Pore 0600
Pore 0600
Pore 0600
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 0900
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1200
Pore 1500
Pore 1500
Pore 1500
Pore 1500
Pore 1500
Pore 1500
7.498
6.397
8.643
4.561
4.008
8.746
8.691
9.443
9.086
12.084
10.069
8.751
8.048
8.947
9.527
10.294
12.574
0.129
0.895
2.556
4.777
0.869
1.025
3.250
3.928
1.962
4.865
3.923
5.888
2.401
2.520
3.912
21.201
21.656
24.795
25.466
9.810
8.082
24.610
28.695
32.601
34.425
38.971
37.027
48.323
42.420
33.250
36.426
27.213
31.299
29.600
30.959
23.346
18.881
23.720
23.354
15.120
13.832
21.765
20.443
22.664
25.613
Pore _1500
Pore _1500
Pore _1800
Pore _1800
Pore _1800
Pore _1800
Pore _1800
Pore _1800
Pore _1800
Pore _1800
7.623
6.597
9.520
10.892
7.749
7.884
8.195
8.464
6.266
4.277
15.909
14.072
25.681
23.255
36.579
35.240
27.848
34.920
Pore 1500
Pore 1500
Pore 1800
Pore 1800
Pore 1800
Pore 1800
Pore 1800
Pore 1800
Pore 1800
Pore 1800
2.479
2.821
16.016
17.548
11.285
11.555
13.795
12.542
149
3.131 25.308
3.238 26.823
3.134
4.426
3.019
3.387
7.178
7.785
6.236
5.943
Appendix 10.
Mesocosm physiochemical measurements and respiration
Treatment
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Substrate
sediment
sediment
sediment
sediment
sediment
leaves
leaves
leaves
leaves
leaves
sponge
sponge
sponge
sponge
sponge
glass
glass
glass
glass
glass
glass
sediment
sediment
sediment
sediment
sediment
DO 1
DO 2
DO 3
pH 1
pH 2
pH 3
Temp 1
Temp 2
Temp 3
7.63
7.73
7.76
7.77
7.69
7.54
7.38
7.36
7.43
7.47
7.61
7.45
7.79
7.86
7.58
7.66
7.61
7.37
7.74
7.39
7.4
7.54
7.52
7.41
7.37
7.41
7.67
7.77
7.8
7.76
7.71
7.68
7.41
7.51
7.66
7.65
7.42
7.49
7.79
7.91
7.64
7.65
7.75
7.45
7.76
7.45
7.51
7.59
7.71
7.47
7.47
7.54
7.74
7.86
7.85
7.97
7.73
7.53
7.4
7.59
7.53
7.47
7.44
7.35
7.87
7.87
7.6
7.6
7.75
7.46
7.69
7.48
7.51
7.64
7.72
7.55
7.47
7.48
8.59
8.64
8.57
8.59
8.54
8.62
8.58
8.42
8.62
8.63
8.55
8.56
8.59
8.6
8.57
8.61
8.56
8.68
8.65
8.61
8.54
8.61
8.64
8.52
8.6
8.61
8.61
8.62
8.56
8.6
8.56
8.61
8.57
8.45
8.59
8.6
8.53
8.55
8.59
8.61
8.56
8.6
8.55
8.63
8.61
8.59
8.58
8.61
8.63
8.56
8.56
8.58
8.69
8.66
8.67
8.68
8.54
8.71
8.64
8.53
8.65
8.64
8.59
8.6
8.64
8.61
8.59
8.7
8.61
8.58
8.58
8.57
8.51
8.63
8.67
8.62
8.6
8.62
20.3
20.4
20.1
20.5
20
20.1
21.3
20.8
20.9
21.3
21.1
21.7
19.9
19.3
20.8
20.6
20.5
21.8
20.1
21.9
21.7
21.2
20.5
21.4
21.5
21.5
19.9
20.2
19.7
20.1
19.7
20.2
21.3
20.3
20.5
21
21.2
21.5
19.6
18.9
20.3
20.3
19.9
21.6
19.7
21.6
21.5
21
20
21.2
21.2
21.5
20.2
19.9
19.6
19.7
19.5
20.6
21.1
20.3
20.8
21.2
21.8
21.8
19.5
18.9
20.7
20
20
21.9
20
21.8
22
20.9
20
21.2
21.4
21.7
150
μg O2 Consumed / h
0.16870
0.10730
-0.02222
0.28817
0.12293
0.72249
0.62982
0.50142
0.85200
0.20331
0.06933
0.08273
0.09390
0.11399
0.06040
0.07715
0.15754
0.06710
0.10060
0.11399
0.08832
0.04700
0.19773
0.15642
0.14191
0.00000
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
leaves
leaves
leaves
leaves
leaves
sponge
sponge
sponge
sponge
sponge
glass
glass
glass
glass
glass
glass
sediment
sediment
sediment
sediment
sediment
leaves
leaves
leaves
leaves
leaves
sponge
sponge
sponge
8.02
7.55
7.72
7.79
7.68
7.58
7.43
7.52
7.6
7.63
7.7
7.7
7.71
7.76
7.65
7.62
7.56
7.44
7.58
7.57
7.53
7.48
7.54
7.49
7.58
7.42
7.53
7.55
7.39
7.89
7.64
7.82
7.94
7.67
7.66
7.55
7.69
7.76
7.62
7.7
7.74
7.77
8.02
7.73
7.75
7.62
7.42
7.55
7.71
7.64
7.51
7.54
7.43
7.64
7.46
7.53
7.54
7.47
8.08
7.65
7.91
7.78
7.61
7.66
7.45
7.56
7.75
7.6
7.78
7.8
7.83
7.77
7.67
7.74
7.61
7.48
7.47
7.64
7.57
7.39
7.51
7.45
7.66
7.42
7.55
7.52
7.46
8.67
8.6
8.59
8.63
8.52
8.56
8.53
8.52
8.5
8.49
8.63
8.59
8.66
8.61
8.56
8.55
8.68
8.61
8.63
8.65
8.62
8.72
8.62
8.65
8.65
8.53
8.6
8.61
8.6
8.66
8.6
8.59
8.65
8.56
8.56
8.51
8.52
8.53
8.53
8.64
8.57
8.65
8.6
8.54
8.56
8.67
8.61
8.62
8.66
8.62
8.71
8.62
8.65
8.61
8.58
8.58
8.59
8.57
8.76
8.68
8.69
8.73
8.61
8.62
8.56
8.52
8.54
8.5
8.69
8.66
8.68
8.69
8.6
8.59
8.68
8.67
8.67
8.69
8.56
8.78
8.72
8.74
8.67
8.6
8.62
8.62
8.59
151
18.7
20.8
19.8
19.3
20.5
20.5
21.1
20.8
20.1
20.6
20.1
20.5
19.9
19.8
20.2
20.2
20.6
21.5
21.2
20.2
21.1
21.5
21
21.5
20.8
21.8
21
20.9
21
18.3
20.3
19.4
18.8
20
19.9
20.9
20.5
19.7
20.5
19.9
20.1
19.6
19.5
19.8
20.1
20.5
21.6
21.3
20
21
21.3
20.9
21.6
20.3
21.4
21
20.7
20.9
18.4
20.3
19.1
19.4
20.5
20
21
20.6
19.5
21.1
19.8
19.9
19.8
19.7
19.8
20.2
20.8
21.7
21.4
20.1
21.5
21.8
21.3
21.6
20.4
21.4
21.4
21.4
21.4
0.57958
0.58962
0.94690
1.06414
1.39685
0.10171
0.14637
0.09836
0.10730
0.20890
0.09948
0.14191
0.11511
0.08273
0.09613
0.03249
0.09725
0.07045
0.16089
0.03696
0.02802
0.67448
0.90224
0.61642
0.95584
1.00720
0.07938
0.08943
0.04031
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
sponge
sponge
glass
glass
glass
glass
glass
glass
sediment
sediment
sediment
sediment
sediment
leaves
leaves
leaves
leaves
leaves
sponge
sponge
sponge
sponge
sponge
glass
glass
glass
glass
glass
glass
7.49
7.47
7.61
7.5
7.45
7.64
7.6
7.64
7.52
7.48
7.83
7.89
7.6
7.63
7.32
7.58
7.49
7.62
7.49
7.32
7.5
7.61
7.52
7.17
7.73
7.98
7.68
7.67
7.53
7.61
7.49
7.57
7.49
7.53
7.64
7.64
7.78
7.55
7.47
7.85
7.95
7.77
7.71
7.31
7.6
7.57
7.72
7.45
7.38
7.54
7.61
7.6
7.62
7.83
7.99
7.78
7.74
7.62
7.46
7.45
7.61
7.44
7.53
7.73
7.56
7.69
7.51
7.47
7.91
7.91
7.56
7.81
7.4
7.58
7.45
7.63
7.46
7.35
7.41
7.6
7.67
7.48
7.69
7.83
7.72
7.74
7.56
8.59
8.56
8.61
8.68
8.66
8.66
8.71
8.71
8.55
8.56
8.58
8.62
8.62
8.64
8.58
8.64
8.58
8.64
8.53
8.49
8.51
8.54
8.51
8.53
8.6
8.66
8.59
8.52
8.51
8.57
8.54
8.61
8.68
8.67
8.66
8.71
8.66
8.54
8.55
8.57
8.61
8.62
8.66
8.57
8.63
8.56
8.65
8.52
8.49
8.5
8.56
8.51
8.55
8.61
8.62
8.54
8.49
8.53
8.54
8.57
8.72
8.63
8.67
8.7
8.62
8.6
8.57
8.6
8.62
8.6
8.61
8.77
8.6
8.71
8.6
8.61
8.56
8.52
8.56
8.6
8.55
8.6
8.59
8.61
8.55
8.51
8.46
152
21.1
21.3
20.8
21.6
21.2
21
20.8
20.1
21.2
21.6
19.4
19.1
20.8
19.7
22
20.9
21.2
20.6
21.3
22.1
21.1
20.6
21.3
21
20.1
18.6
20.1
20.2
21.3
21.2
21.1
20.9
21.5
21.1
20.8
20.3
19.8
21.3
21.5
19
18.8
20.5
19.4
21.6
20.6
20.9
20.6
21.2
22.1
21
20.4
21.1
20.9
19.9
17.8
19.8
19.9
20.9
21.6
21.4
21.2
21.9
21.3
20.5
20.5
20.3
21.2
21.4
19
19
20.6
19.6
21.7
21
21.2
21
21.4
22.3
21.4
20.6
20.7
21.4
20
18.9
19.8
19.7
21.3
0.17875
0.03584
0.13744
0.16312
0.09278
0.05482
0.10171
0.06152
0.07268
0.12851
0.04477
0.05259
0.09390
0.43108
0.87880
-0.18299
0.61530
1.55875
0.06822
0.05817
0.10395
0.03472
0.04589
0.09390
0.23123
0.05705
0.08273
0.04700
0.06040
Appendix 11.
Mesocosm dissolved nutrient and total organic carbon concentrations
Treatment Substrate
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Control
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Leaves
Leaves
Leaves
Leaves
Sediment
Sediment
Sediment
Sediment
Sponge
Sponge
Sponge
Sponge
Glass
Glass
Glass
Glass
Leaves
Leaves
Leaves
Leaves
Sediment
Sediment
Sediment
Sediment
Sponge
Day 1
mg NO3 / L
5.785
3.555
3.467
4.460
8.489
8.355
4.855
5.191
0.166
0.149
0.579
0.542
8.371
8.194
7.310
6.992
5.579
5.456
3.478
3.680
6.394
6.405
7.424
7.426
0.093
Day 2
mg NO3 / L
6.862
6.508
6.812
6.687
9.868
10.001
11.236
11.034
1.704
1.679
1.782
1.789
10.928
11.320
11.299
11.175
6.301
6.164
0.804
0.787
9.871
9.964
9.877
9.999
1.612
Day 1
mg PO4 / L
0.017
0.032
0.015
0.003
0.003
0.003
0.000
0.000
0.001
0.005
0.005
0.001
0.001
0.000
0.009
0.000
0.004
0.020
0.008
0.002
0.022
0.078
0.060
0.054
0.041
Day 2
mg PO4 / L
0.023
0.014
0.017
0.022
0.003
0.001
0.004
0.000
0.000
0.000
0.000
0.000
0.003
0.001
0.000
0.001
0.014
0.017
0.004
0.002
0.000
0.001
0.000
0.000
0.003
153
Day 1
mg TOC / L
8.630
8.317
Day 2
mg TOC / L
8.710
9.836
5.631
5.756
5.584
5.290
6.718
6.075
6.138
6.212
6.053
5.763
5.032
4.954
10.060
10.420
10.440
9.355
7.879
7.540
7.653
7.714
9.513
8.388
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Exudate
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Sponge
Sponge
Sponge
Glass
Glass
Glass
Glass
Leaves
Leaves
Leaves
Leaves
Sediment
Sediment
Sediment
Sediment
Sponge
Sponge
Sponge
Sponge
Glass
Glass
Glass
Glass
Leaves
Leaves
Leaves
Leaves
Sediment
Sediment
0.092
0.040
0.014
5.810
5.912
7.269
7.539
3.869
3.846
4.825
3.667
7.103
7.040
7.674
7.536
0.264
0.269
1.222
1.186
5.364
5.424
9.470
9.340
7.325
7.434
6.534
6.596
7.332
7.064
1.698
1.210
1.194
9.307
9.111
8.129
8.139
6.294
6.352
5.827
5.908
8.908
8.872
7.872
7.923
2.785
2.897
2.869
2.900
11.826
12.085
8.683
8.387
10.520
10.461
10.131
10.803
8.422
8.092
0.000
0.002
0.012
0.045
0.006
0.017
0.015
0.001
0.004
0.027
0.040
0.000
0.000
0.000
0.001
0.003
0.002
0.000
0.000
0.000
0.004
0.005
0.001
0.007
0.001
0.024
0.018
0.000
0.000
0.001
0.000
0.000
0.001
0.000
0.001
0.000
0.029
0.029
0.007
0.006
0.001
0.000
0.002
0.002
0.000
0.001
0.001
0.002
0.001
0.002
0.000
0.001
0.013
0.010
0.017
0.015
0.000
0.000
154
8.539
8.513
7.134
8.005
7.092
7.035
8.600
7.717
8.414
8.183
5.016
5.323
6.027
4.897
6.646
6.847
6.556
6.468
5.422
5.500
5.149
5.816
7.912
9.203
8.129
7.940
5.226
5.078
5.268
4.753
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Algae
Sediment
Sediment
Sponge
Sponge
Sponge
Sponge
Glass
Glass
Glass
Glass
5.979
6.014
0.204
0.204
0.568
0.630
6.818
6.734
6.832
6.840
9.156
8.954
3.978
3.972
2.195
2.199
9.599
9.360
10.900
10.154
0.002
0.001
0.002
0.001
0.000
0.000
0.009
0.009
0.005
0.000
0.000
0.003
0.000
0.000
0.002
0.000
0.002
0.000
0.000
0.000
155
6.652
6.952
6.589
5.889
6.274
5.478
5.534
5.984