I. Punnett Squares Punnett Squares & Probability How to figure out what the probable outcome of a genetic cross will be A. Vocabulary 1. Genotype= Genotype= genetic makeup, what the genes say 2. Phenotype= Phenotype= physical appearance, how it looks 3. Genotypic ratio= ratio= (3 #s) homozygous dominant: dominant: heterozygous: heterozygous: homozygous recessive 4. Phenotypic ratio= ratio= (2 #s) dominant appearance : recessive appearance B. Punnett Square Procedure 1. 2. 3. 4. 5. Figure out the cross Write down what the cross is Draw & label the Punnett Square Fill in the squares Write down the genotypic & phenotypic ratios D. Example 1 Let T = tall pea plant Let t = short pea plant Cross a short pea plant with a heterozygous pea plant C. Punnett Square Conventions 1. A capital letter stands for the dominant trait (lower case = recessive) recessive) 2. If there is a capital letter, write it first 3. First parent mentioned is the ♂. Write that genotype at top of Punnett. Punnett. D. Example 1 3. Draw and label square 1. What is the cross? 2. tt x Tt http://users.adelphia.net/~lubehawk/BioHELP!/psquare.htm http://users.adelphia.net/~lubehawk/BioHELP!/psquare.htm 1 D. Example 1 4. Fill in the squares D. Example 1 4. Fill in the squares D. Example 1 4. Fill in the squares D. Example 1 5. Figure out the genotypic ratio Homozygous dominant = none Heterozygous = 2 Homozygous recessive = 2 genotypic ratio = none : 2 : 2 D. Example 1 Figure out the phenotypic ratio Dominant appearance: recessive appearance 2:2 reduce to 1 : 1 E. Example 2 Cross two pea plants heterozygous for tallness 1. Tt x Tt 2. Draw and label square T t T t 2 E. Example 2 3. Fill in squares T 4. Genotypic & Phenotypic ratios t gr = T TT Tt t Tt tt 1:2:1 pr = 3:1 Statements about Probability 2. Previous events do not affect future outcomes. II. Probability A. Probabilty = the likelihood that a particular event will occur B. Statements about probability 1. probability = # of times an event occurs # of trials Bibliography Punnett baby steps http://users.adelphia.net/~lubehawk/BioHELP!/psquare.htm 11/4/07 3. The larger the number of trials, the closer one gets to the expected probability. 3