Punnett Squares & Probability How to figure out what the probable outcome of a genetic cross will be I. Punnett Squares A. Vocabulary 1. Genotype= genetic makeup, what the genes say 2. Phenotype= physical appearance, how it looks 3. Genotypic ratio= (3 #s) homozygous dominant: heterozygous: homozygous recessive 4. Phenotypic ratio= (2 #s) dominant appearance : recessive appearance B. Punnett Square Procedure 1. 2. 3. 4. 5. Figure out the cross Write down what the cross is Draw & label the Punnett Square Fill in the squares Write down the genotypic & phenotypic ratios C. Punnett Square Conventions 1. A capital letter stands for the dominant trait (lower case = recessive) 2. If there is a capital letter, write it first 3. First parent mentioned is the ♂. Write that genotype at top of Punnett. D. Example 1 Let T = tall pea plant Let t = short pea plant Cross a heterozygous pea plant with a short pea plant 1. What is the cross? 2. Tt x tt D. Example 1 3. Draw and label square http://users.adelphia.net/~lubehawk/BioHELP!/psquare.htm D. Example 1 4. Fill in the squares D. Example 1 4. Fill in the squares D. Example 1 4. Fill in the squares D. Example 1 5. Figure out the genotypic ratio Homozygous dominant = none Heterozygous = 2 Homozygous recessive = 2 genotypic ratio = none : 2 : 2 D. Example 1 Figure out the phenotypic ratio Dominant appearance: recessive appearance 2:2 reduce to 1 : 1 E. Example 2 Cross two pea plants heterozygous for tallness 1. Tt x Tt 2. Draw and label square T T t t E. Example 2 3. Fill in squares T 4. Genotypic & Phenotypic ratios t gr = T TT Tt 1:2:1 pr = 3:1 t Tt tt II. Probability A. Probabilty = the likelihood that a particular event will occur B. Statements about probability 1. probability = # of times an event occurs # of trials Statements about Probability 2. Previous events do not affect future outcomes. 3. The larger the number of trials, the closer one gets to the expected probability. Bibliography Punnett baby steps http://users.adelphia.net/~lubehawk/BioHELP!/psquare.htm 11/4/07