Anthracnose Stalk Rot Resistance
from GEM Germplasm
Margaret Smith
Department of Plant Breeding
Cornell University
College of Agriculture and Life Sciences
Department of Plant Breeding
Anthracnose Stalk Rot
• Colletotrichum graminicola
(Ces.) G.W. Wils.
• Increased lodging, reduced yield
• Best control through resistant
varieties
• Limited resistance in temperate
germplasm
College of Agriculture and Life Sciences
Department of Plant Breeding
Overall Project Objective
Develop temperate-adapted maize inbreds
with both anthracnose stalk rot resistance and
good yield potential
from GEM breeding populations
College of Agriculture and Life Sciences
Department of Plant Breeding
Selection and Breeding Scheme
Evaluate 30 populations
for ASR resistance
Testcross and evaluate
yield potential and adaptation
Self pollinate and
screen for ASR resistance
College of Agriculture and Life Sciences
Department of Plant Breeding
Testcross to monitor
yield and adaptation
Selected Populations
1)
2)
3)
4)
FS8B(T):N1802
CH04030:S0906
AR01150:N0406
GOQUEEN:N1603
College of Agriculture and Life Sciences
Department of Plant Breeding
Means by source population for
anthracnose stalk rot ratings, 2001
Population
FS8B(T):N1802
CH04030 :S0906
AR01150 :N0406
GOQUEEN:N1603
Means of S5
Means of s elected
famil y ratings (%)
S5 plant rating s (%)
No.
Mean
No.
Mean
Famili es (± s.d.)
Plants (± s.d.)
6 11.7 (± 1.8)
6 14.0 (± 5.3)
9 11.5 (± 1.6) 12 12.6 (± 7.5)
6 16.1 (± 4.2)
9 14.5 (± 7.4)
9 16.0 (± 6.6) 13 10.6 (± 9.4)
Resis. che ck (01/31*)
48.0
LB31B
50.6
Susc . che ck (B37)
73.2
* Our most resistant inbred at present, currently being released.
College of Agriculture and Life Sciences
Department of Plant Breeding
Yield Data - 2 locations, 2001
S2 Family
FS8B(T):N1802-490
FS8B(T):N1802-491
FS8B(T):N1802-492
FS8B(T):N1802-493
CH04030:S0906-476
CH04030:S0906-477
CH04030:S0906-478
AR01150:N0406-496
Tester
Yield
(bu/A)
Moist.
(%)
Y/M
Ratio
B73/CD1
“
“
“
RD6501/
RD6502
“
B73/CD1
118.5
122.8
119.7
123.5
118.5
120.1
112.5
112.9
21.9
21.7
26.0
25.0
22.7
21.7
21.8
20.8
5.6
5.8
4.7
5.0
5.2
5.6
5.2
5.5
0
1
0
0
0
0
0
0
19
16
13
16
45
31
24
43
166.1
128.1
125.3
20.0
25.0
21.1
8.4
5.2
6.0
8
0
0
7
7
15
13.3
5.5
Pioneer 34G81
Agway 795
Doeblers 596XY
Coefficient of variation (%)
College of Agriculture and Life Sciences
Department of Plant Breeding
Lodging (%)
Root Stalk
Yield Data - 1 location, 2001
S2 Family
Tester
Yield
(bu/A)
Moist. Y/M
(%) Ratio
Lodging (%)
Root Stalk
AR01150:N0406-498 B73/CD1 164.1
AR01150:N0406-499
“
187.5
GOQUEEN:N1603-510
“
197.7
GOQUEEN:N1603-511
“
194.0
22.0
21.8
20.1
19.0
7.5
8.6
9.9
10.2
0
0
0
0
3
8
3
6
Pioneer 34G81
Agway 657
Agway 795
Doeblers 596XY
214.5
193.3
183.6
169.5
19.6
20.5
24.0
20.2
10.9
9.4
7.6
8.4
0
0
0
0
0
4
3
3
10.8
4.9
Coefficient of variation (%)
College of Agriculture and Life Sciences
Department of Plant Breeding
Work done in 2002…
• S6 progenies planted ear-to-row
– Greater emphasis on yield performance
– Greater tolerance of stalk rot resistance
• Testcrosses made to:
– Previously used public line crosses
– Holden’s testers (LH176/LH177, LH198)
College of Agriculture and Life Sciences
Department of Plant Breeding
Anthracnose Stalk Rot Resistance
in a Few GEM Inbreds, 2002
Inbred
FS8B(T):N1802 15-255
FS8B(T):N1802 15-259
CH04030 :S0906 15-242
GOQUEEN:N1603 15-276
RD02 LB1-2 (resis. check)
DE811 ASR (resis. check)
RD6501 (resis. check)
NYLB31B (resis. check )
B37 (suscep. check )
Mean of injected
internode (%)
78.3
90.7
90.3
68.5
56.2
42.6
61.3
86.8
91.4
College of Agriculture and Life Sciences
Department of Plant Breeding
Internod es 2 through 8 (%)
Plant
Plant
Mean (± s.d.)
Min.
Max.
9.4 (± 9.4)
0.2
25.3
23.2 (± 13.8)
7.6
46.0
15.7 (± 10.4)
1.8
28.9
7.1 (± 5.5)
0.2
17.0
5.2 (± 5.6)
0.2
15.3
9.1 (± 7.8)
1.3
21.9
16.3 (± 2.9)
14.5
19.6
26.5 (± 17.5)
2.0
49.4
70.6 (± 9.1)
58.9
83.1
FS8B(T):N1802 15-255
DE811ASR B37
College of Agriculture and Life Sciences
Department of Plant Breeding
FS8B(T):N1802
15-255
DE811ASR B37
College of Agriculture and Life Sciences
Department of Plant Breeding
Yields of a Few GEM Inbreds
Tested on B73/CD1, 2001, 2002
S5 Famil y
FS8B(T):N1802-490
FS8B(T):N1802-491
GOQUEEN:N1603-512
GOQUEEN:N1603-510
GOQUEEN:N1603-511
Yield
(bu/A)
129.1
129.2
146.3
133.9
133.9
Moist ure
(%)
25.4
26.2
23.9
23.9
23.1
Y/M
Ratio
5.1
4.9
6.1
5.6
5.8
Pionee r 34G81
Doeblers 596XY
170.5
156.5
23.4
25.2
7.3
6.2
13.8
2.4
Coefficient of var iation (%)
College of Agriculture and Life Sciences
Department of Plant Breeding
Root
Stalk
Lodge ( %) Lodge ( %)
3
15
11
13
3
16
8
14
7
20
2
2
7
13
Work done in 2003…
• S6 progenies and their testcrosses (public
and Holden’s testers) evaluated for
anthracnose stalk rot resistance (2 reps)
• Testcrosses evaluated in yield trials in 3
New York locations (2 reps/location)
• Results: just in from the field -- stay tuned!
College of Agriculture and Life Sciences
Department of Plant Breeding
Thank you!
College of Agriculture and Life Sciences
Department of Plant Breeding
Questions?