Developing Breeding Lines with
Anthracnose Stalk Rot Resistance
from Exotic Maize Germplasm
Margaret Smith
College of Agriculture and Life Sciences
Department of Plant Breeding
Anthracnose Stalk Rot
• Colletotrichum
graminicola (Ces.)
G.W. Wils.
• Increased lodging,
reduced yield
• Best control through
resistant varieties
College of Agriculture and Life Sciences
Department of Plant Breeding
Overall Project Objective
Develop temperate-adapted maize inbreds
with both anthracnose stalk rot resistance and
good yield potential
from GEM breeding populations
College of Agriculture and Life Sciences
Department of Plant Breeding
Selection and Breeding Scheme
ASR screening of GEM Populations
(1995)
Selected 5 most resistant
Testcross yield evaluation of these 5 populations
(1996)
Selected 4 best yielding 25% exotic : 75% temperate populations
Selfing within the 5 selected populations
(1996)
Made about 100 selfs in 25% exotic : 75% temperate populations
Selfing and selection in S1 families
(1997)
50 S1 families per population
Selfed and injected 8 plants/S1 family
Selfing and selection in S2 families
(1998)
Selfed and injected 8 plants/S2 family
Testcrosses of S2 families
(1998)
Crossed to SS or nSS public line cross testers
Selfing and selection in S3 families
(1999)
Selfed and injected 8 plants/S3 family
Yield trials of S2 x tester crosses
(1999)
3 locations, 2 reps/location
Repeat for S4 families
(2000) and
S5 families (2001)
Repeat for S4 x tester crosses
(2000 - 3 locations, 2 reps/loc.)
and S5 x tester crosses
(2001 - 2 locations, 2 reps/loc.)
Testcrosses of broader selection of S6 families
(2002)
Used both public and Holden's testers
College of Agriculture and Life Sciences
Department of Plant Breeding
Selected Populations
1)
2)
3)
4)
FS8B(T):N1802
CH04030:S0906
AR01150:N0406
GOQUEEN:N1603
College of Agriculture and Life Sciences
Department of Plant Breeding
Means by source population for
anthracnose stalk rot ratings, 2001
Population
FS8B(T):N1802
CH04030 :S0906
AR01150 :N0406
GOQUEEN:N1603
Means of S5
Means of s elected
famil y ratings (%)
S5 plant rating s (%)
No.
Mean
No.
Mean
Famili es (± s.d.)
Plants (± s.d.)
6 11.7 (± 1.8)
6 14.0 (± 5.3)
9 11.5 (± 1.6) 12 12.6 (± 7.5)
6 16.1 (± 4.2)
9 14.5 (± 7.4)
9 16.0 (± 6.6) 13 10.6 (± 9.4)
Resis. che ck (01/31*)
48.0
LB31B
50.6
Susc . che ck (B37)
73.2
* Our most resistant inbred at present, currently being released.
College of Agriculture and Life Sciences
Department of Plant Breeding
Yield Data - 2 locations, 2001
S2 Family
FS8B(T):N1802-490
FS8B(T):N1802-491
FS8B(T):N1802-492
FS8B(T):N1802-493
CH04030:S0906-476
CH04030:S0906-477
CH04030:S0906-478
AR01150:N0406-496
Tester
Yield
(bu/A)
Moist.
(%)
Y/M
Ratio
B73/CD1
“
“
“
RD6501/
RD6502
“
B73/CD1
118.5
122.8
119.7
123.5
118.5
120.1
112.5
112.9
21.9
21.7
26.0
25.0
22.7
21.7
21.8
20.8
5.6
5.8
4.7
5.0
5.2
5.6
5.2
5.5
0
1
0
0
0
0
0
0
19
16
13
16
45
31
24
43
166.1
128.1
125.3
20.0
25.0
21.1
8.4
5.2
6.0
8
0
0
7
7
15
13.3
5.5
Pioneer 34G81
Agway 795
Doeblers 596XY
Coefficient of variation (%)
College of Agriculture and Life Sciences
Department of Plant Breeding
Lodging (%)
Root Stalk
Yield Data - 1 location, 2001
S2 Family
Tester
Yield
(bu/A)
Moist. Y/M
(%) Ratio
Lodging (%)
Root Stalk
AR01150:N0406-498 B73/CD1 164.1
AR01150:N0406-499
“
187.5
GOQUEEN:N1603-510
“
197.7
GOQUEEN:N1603-511
“
194.0
22.0
21.8
20.1
19.0
7.5
8.6
9.9
10.2
0
0
0
0
3
8
3
6
Pioneer 34G81
Agway 657
Agway 795
Doeblers 596XY
214.5
193.3
183.6
169.5
19.6
20.5
24.0
20.2
10.9
9.4
7.6
8.4
0
0
0
0
0
4
3
3
10.8
4.9
Coefficient of variation (%)
College of Agriculture and Life Sciences
Department of Plant Breeding
Work done in 2002…
• S6 progenies planted ear-to-row
– Greater emphasis on yield performance
– Greater tolerance of stalk rot resistance
• Testcrosses made to:
– Previously used public line crosses
– Holden’s testers (LH176/LH177, LH198)
College of Agriculture and Life Sciences
Department of Plant Breeding