Petunia nectar proteins have ribonuclease activity
Melissa S. Hillwig, Xiaoteng Liu, Guangyu Liu, Robert W. Thornburg,
and Gustavo C. MacIntosh
Supplemental Table 1. Primers used in this work
Cloning / RT-PCR
RNase Phy1 -Forward
RNase Phy1 -Reverse
RNase Phy3 -Forward
RNase Phy3 -Reverse
RNase Phy4 -Forward
RNase Phy4 -Reverse
RNase Phy5 -Forward
RNase Phy5 -Reverse
18s - Forward
18s - Reverse
5' -> 3'
GGCGTCAATATTTGCCACTTCTATG
CGATTCCAGGAGTAAATCCAGTTG
CTGAATTGCTCGGAACCAAAATTAAC
TGACAATATTTTTGGGGGACGGC
GTAGTAAGCCGGACACAAAGTCATGC
CCTGTTAGTTGTACCTGTCCCGTTC
ATTGTGATACAAGGCGTAGTTGCTG
GAGAAAGGAGGGAATTCAATCTTAG
ACTAATTCAGACTGTGAAACTGCGA
CTTGCTTTGAGCACTCTAATTTCT
RACE
RNase Phy3 -Forward
RNase Phy3 -Reverse
RNase Phy4 -Forward
RNase Phy4 -Reverse
5' -> 3'
TGGTACGGATGAGGGGTTCTGGATACATG
GGACGGCCCACAACCTCTATTAGCATGAG
GGCCAGCAAATGCATACGCGAACAGTTTG
CCAAGGGGTTGAGAGCTCTCCCAAGTTGAC
Petunia nectar proteins have ribonuclease activity
Melissa S. Hillwig, Xiaoteng Liu, Guangyu Liu, Robert W. Thornburg, and Gustavo C. MacIntosh
Supplemental Figure 1.
5.5
5
Relative growth
(OD Tx / OD T0)
4.5
4
3.5
3
2.5
2
1.5
1
LxS8 1:2 LxS8 1:6 Pet 1:2
Pet 1:6
LB
Effect of tobacco and petunia nectar on bacterial growth. Growth of Pseudomonas fluorescens
(strain A506) in 1:2 or 1:6 dilutions of nectar from Petunia hybrida (Pet) and the ornamental
tobacco hybrid LxS8 (Nicotiana langsdorffii x Nicotiana sanderae var LxS8) was determined
after an overnight culture by changes in OD. Bacterial growth on LB medium is shown for
comparison.
Petunia nectar proteins have ribonuclease activity
Melissa S. Hillwig, Xiaoteng Liu, Guangyu Liu, Robert W. Thornburg, and Gustavo C. MacIntosh
Supplemental Figure 2.
Ribonuclease activities are present in nectar. a. Aliquots (5 μg) of raw nectar from Petunia
hybrida and ornamental tobacco (Nicotiana langsdorffii x Nicotiana sanderae var LxS8) were
analyzed in an in gel RNase activity assay. P, petunia; L, LxS8. Size (kDa) of molecular weight
markers (M) is indicated. b. Same samples as in a, analyzed by SDS-PAGE, and stained with
Coomassie Blue. Gels are representative of at least 3 independent experiments.