AcroPrep Membrane-bottom Filter Plates Multi-well, Membrane-bottom Plates for

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Product Data
AcroPrep™ Membrane-bottom
Filter Plates
Multi-well, Membrane-bottom Plates for
High Throughput Sample Preparation
and Detection Procedures
• Broad selection – available in 96- or 384-well
formats with a variety of membranes, plate color
choices, well volumes, and outlet tip lengths for
use in a multitude of sample preparation and
detection processes.
• Automation friendly – designed in accordance
with the ANSI/Society for Biomolecular Screening
(SBS) standards. Rigid construction enables the
plate to be easily maneuvered by robotic
equipment and assures that plates do not flex
and jam detectors or other instrumentation.
• Low binding, chemically resistant –
polypropylene housing assembly is compatible
with a broad range of aqueous and organic
liquids, and is noted for its low biomolecule
binding so that non-specific adsorption to the
plate is minimized.
• Serialized barcodes – serialized barcode labels
allow for the use of automated tracking systems.
• No crosstalk – specially engineered fluid
deflectors and outlet tips on the bottom of
the plate were designed to reduce the potential
for crosstalk. Elimination of crosstalk is assured
through individually sealing a membrane in each
well using proprietary sealing technology.
Applications
•
•
•
•
•
•
•
•
•
•
Sample prep/gross fractionation
DNA purification, concentration, and fractionation
Receptor:ligand binding assays
Prefiltration
Clarification of cell lysates and tissue homogenates
Preparation of proteinaceous solutions
Drug binding studies
Low protein or nucleic acid binding procedures
Chemiluminescence detection
Radioactive (isotopic) detection
•
•
•
•
•
•
•
•
Fluorescence detection
Combinatorial chemistry library screening
Cleavage assays
Bead-based assays
Protein purification, concentration, and fractionation
Capture from liquid chromatography
Antibody purification
Contaminant removal such as DNA, RNA, or other
charged molecules
Plate Selection
Low Biomolecule Binding
Solvent Resistance Required
Hydrophobic Membrane
(Solvent Based Solutions)
PTFE Membrane
Applications
• Molecular Synthesis Reactions
• Drug Synthesis Reactions
• Resin Cleavage Reactions
• Combi-chem Library Screening
• Sample Prep Involving Solvents
96 Well
350 µL
1 mL
0.2 µm
0.45 µm
0.2 µm
0.45 µm
Natural Housing
Natural Housing
Natural Housing
Natural Housing
PN 5037
PN 5038
PN 5055
PN 5056
Low Biomolecule Binding
Solvent Resistance Not Required
Integrated Prefilter
Polypropylene Prefilter
Applications
• Debris Removal
• Low Salt DNA Purification
• Rapid Plasmid Preparation
• Proteinaceous Sample
Purification
• Tissue Homgenates
Glass Fiber Prefilter
Applications
• Plasmid Preparation
• Lysate Clearance
• Protein Solutions
96 Well
96 Well
1 mL
350 µL
350 µL
Polypropylene/1.2 µm
Supor® Membrane
3.0 µm GF/0.2 µm
Bio-Inert® Membrane
3.0 µm GF/0.2 µm
Bio-Inert Membrane
PN 5041
PN 5046
PN 5053
Hydrophilic Membrane
(Aqueous or Solvent Based Solutions)
GHP Membrane
(Hydrophilic Polypropylene)
Applications
• Cleavage Reactions
• In-gel Digestion
• Combi-chem Library Screening
• Drug Binding Studies
• Sample Prep
• Bead/Resin Applications
384 Well
96 Well
350 µL
0.2 µm
1 mL
0.2 µm
0.45 µm
0.45 µm
Natural Housing
Natural Housing
Natural Housing
PN 5052
PN 5054
0.45 µm
Natural Housing
PN 5045
Natural Housing
White Housing
Black Housing
PN 5030
PN 5043
PN 5044
100 µL
Short Tip
Long Tip
PN 5071
PN 5070
No Prefilter
Polyester Screen
Applications
• Sieve Filtration
• Cytometry Sample Preparation
Bio-Inert Membrane
Supor® Membrane
GHP Membrane
(Hydrophilic Nylon)
(Modified PES)
(Hydrophilic Polypropylene)
Applications
• Sample Prep
• Drug Binding Studies
• Purification of Oligos from
Polyacrylamide Gels
• Bead Assays and Applications
• Resin-based Purifications
Applications
• Sample Prep
• Drug Binding Studies
• Purification of Oligos from
Polyacrylamide Gels
• Resin-based Purifications
• Lysate Clearance
Applications
• Cleavage Reactions
• In-gel Digestion
• Combi-chem Library Screening
• Drug Binding Studies
• Sample Prep
• Bead/Resin Applications
96 Well
96 Well
96 Well
96 Well
384 Well
350 µL
350 µL
350 µL
1 mL
100 µL
30-40 µm
0.2 µm
1.2 µm
1.2 µm
PN 7267
PN 5042
0.45 µm
1.2 µm
PN 5029 PN 5039, 5039L PN 5065
See Above
Short Tip
Long Tip
PN 5085
PN 5084
www.pall.com/lab
Plate Selection
High Biomolecule Binding
Binding Media
Glass Fiber
Applications
• Receptor Ligand Binding Studies
• Cell-based Assays
• Sample Prep
• Gross Fractionation
96 Well
384 Well
1 mL
350 µL
1.0 µm
Natural Housing
PN 5031, 5031L
100 µL
1.0 µm
1.0 µm
1.0 µm
1.0 µm
White Housing
Long Tip
Natural Housing
White Housing
PN 5032
PN 5051
Short Tip
Long Tip
Short Tip
Long Tip
PN 5073
PN 5072
PN 5073W
PN 5072W
Ion Exchange Separations
Membrane Chromatography
Mustang™ Membrane
Applications
• Fractionation of Proteins/Peptides
• Capture from Liquid Chromatography
• Antibody Purification
• Contaminant Removal Such as DNA, RNA,
or Other Charged Molecules
96 Well
1 mL
350 µL
Mustang S
(Cation Exchange)
Mustang Q
(Anion Exchange)
Mustang S
(Cation Exchange)
Mustang Q
(Anion Exchange)
PN 5048
PN 5047
PN 5063
PN 5062
Molecular Separations
Ultrafiltration
Polyethersulfone Membrane
(OmegaTM Membrane)
Applications
• Concentrate, Desalt, and Purify DNA, RNA,
Oligos, Proteins, and Peptides
• Size Exclusion Applications
• Clean-up of Labeling Reactions
• Determination of Free vs. Bound Drug in Plasma
• Purification of Nucleic Acids and Proteins
96 Well
350 µL
1 mL
3K
384 Well
100 µL
3K
10K
30K
100K
PN 5033
PN 5034
PN 5035
PN 5036
10K
30K
100K
Long Tip
Short Tip
Long Tip
Short Tip
Long Tip
Short Tip
Long Tip
PN 5066
PN 5077
PN 5076
PN 5079
PN 5078
PN 5081
PN 5080
Plate Color Selection Guide
Detection results can be further optimized by selecting the
proper plate color. Use the following recommendations as
a guideline for easy selection.
Natural
(semi-opaque)
– fluorescence, TRF
White
(opaque)
– radioactivity,
chemiluminescence
Black
(opaque)
– lowest background for
fluorescence, TRF
www.pall.com/lab
Specifications
AcroPrep™ 96 Filter Plates, 350 µL Wells
Materials of Construction
Housing: Polypropylene (natural, white, or black)
Lid: Polystyrene
Filter Media:
0.2 and 0.45 µm GHP membrane (hydrophilic polypropylene)
0.2 and 0.45 µm PTFE membrane (hydrophobic PTFE
on polyester support)
3.0 µm Glass Fiber/0.2 µm Bio-Inert® membrane
(modified hydrophilic nylon)
0.2 µm Bio-Inert membrane (modified hydrophilic nylon)
1.0 µm (nominal) Glass Fiber (borosilicate glass without
binder over polypropylene support)
3, 10, 30, and 100K Omega™ membrane
(modified polyethersulfone)
0.45 and 1.2 µm Supor® membrane (hydrophilic
polyethersulfone)
0.8 µm Mustang™ Q and S membranes
(hydrophilic polyethersulfone)
30-40 µm polyester screen
AcroPrep 96 Well Plates, 1 mL Wells
Materials of Construction
Housing: Polypropylene (natural)
Filter Media:
0.2 and 0.45 µm GHP membrane (hydrophilic polypropylene)
0.2 and 0.45 µm PTFE membrane (hydrophobic PTFE
on polyester support)
3.0 µm Glass Fiber/0.2 µm Bio-Inert membrane
(modified hydrophilic nylon)
1 µm (nominal) Glass Fiber (borosilicate glass without
binder over polypropylene support)
0.8 µm Mustang Q and S membranes
(hydrophilic polyethersulfone)
3K Omega membrane (modified polyethersulfone)
1.2 µm Supor membrane (hydrophilic polyethersulfone)
Dimensions
Length: 12.78 cm (5.03 in.)
Width: 8.55 cm (3.37 in.)
Height (without lid): 1.44 cm (0.57 in.)
Height (with lid): 1.66 cm (0.66 in.)
Well-bottom Area: 0.25 cm2
Maximum Well Volume
380 µL
Recommended Working Volume
300 µL
Recommended Operating Vacuum
25.4 cm Hg (10 in. Hg) or greater
Filtration by Centrifugation
500-3,000 x g
Typical Sample Hold-up Volume
< 15 µL/well
Dimensions
Length: 12.78 cm (5.03 in.)
Width: 8.55 cm (3.37 in.)
Height (without lid): 2.87 cm (1.13 in.)
Tip Length: 0.53 cm (0.21 in.)
Well-bottom area: 0.25 cm2
Maximum Well Volume
1 mL
Recommended Working Volume
900 µL
Recommended Operating Vacuum
25.4 cm Hg (10 in. Hg) or greater
Filtration by Centrifugation
1,000-3,000 x g
Typical Sample Hold-up Volume
< 27 µL/well
AcroPrep 384 Well Plates, 100 µL Wells
Materials of Construction
Housing: Polypropylene (natural)
Lid: Polystyrene
Media:
0.45 µm GHP membrane (hydrophilic polypropylene)
1 µm (nominal) Glass Fiber (borosilicate glass without binder
over polypropylene support)
1.2 µm Supor (hydrophilic polyethersulfone)
10, 30, and 100K Omega ultrafiltration membrane
(modified polyethersulfone)
Dimensions
Length: 12.78 cm (5.03 in.)
Width: 8.55 cm (3.37 in.)
Height (with lid): 1.66 cm (0.66 in.)
Height (without lid): 1.44 cm (0.57 in.)
Long Tip Length: 0.41 cm (0.16 in.)
Total Plate Height Including Long Tips: 1.85 cm (0.73 in.)
Well-bottom Area: 0.05 cm2
Maximum Well Volume
100 µL
Recommended Working Volume
80 µL
Recommended Operating Vacuum
50.8 cm Hg (20 in. Hg) or greater
Filtration by Centrifugation
500-3,000 x g
Typical Sample Hold-up Volume
< 4 µL per well
Methodology
Filtration vs. Retention
1. Place filter plate on vacuum manifold or hold the plate
so that the outlet tips are not touched.
3a. Release vacuum from the manifold. Remove filter plate
and retained sample for further processing.
2. Add sample and incubate. Apply vacuum.
OR
3b. Release vacuum from the manifold. Remove filter
plate. Remove collection (receiver) plate and utilize
collected filtrate in downstream applications.
AcroPrep™ Filter Plate Operating Instructions
AcroPrep filter plates are ideally suited for both manual use and high-volume robotics applications.
Manual Vacuum Filtration Procedures
1. During use, hold the plate so that the outlets on the
bottom of the plate are not touched. This prevents
liquid flow due to wicking.
2. Add the solution to be filtered and incubate. Place
the plate on the vacuum manifold and apply vacuum.
(See product specifications for recommended vacuum.)
3. Release vacuum from the manifold. DO NOT RELEASE
VACUUM BY PULLING THE CORNER OF THE PLATE
AS IT WILL DEGRADE THE MANIFOLD GASKET.
Robotic Vacuum Filtration Procedure
1. AcroPrep filter plates can be stacked with or without
lids in place.
2. Process the plates in the robotic equipment in the
same way as a standard solid-bottom plate.
3. Transfer the AcroPrep filter plates to a vacuum
manifold station and apply the vacuum. (See product
specifications for recommended vacuum.)
4. Gently tap the plate to remove any hanging droplets.
5. Add wash solution(s) and filter as needed.
www.pall.com/lab
Methodology (continued)
Compatible Robotic Systems
AcroPrep™ filter plates offer a robust platform for sample preparation and detection applications and are compatible
with a variety of currently available robotic systems. Following is a short list of just some of the compatible equipment.
Liquid Handlers
• Adept Technology, Inc.
• Beckman Technologies
• Cyber Lab
• Hamilton Company
• Matrix Technologies Corp.
• Oyster Bay Pump Works, Inc.
• PerkinElmer Inc.
• Porvair
• Qiagen, Inc
• Robbins Scientific
Corp.
• Robocon
• Tecan U.S.
• Titantele
• Tomtec
• Velocity 11
• Waters
Vacuum Manifolds
• Pall Life Sciences
• 3M
• Innovative Microplates
• Millipore
• Porvair
• Qiagen, Inc.
• Whatman
Note: Polypropylene is an inert material with low
biomolecule binding. This is important to prevent nonspecific binding. If a small amount of binding still occurs,
classical passivation procedures can be used (see
Nanosep® Application Guide – PN 32989).
Performance
Figure 1
Lower Level of False Positives with AcroPrep 96 Filter Plates Than Competitive Plates
Pall
AcroPrep 96
Competitor M
3 µg/mL
PE-Ab
47
24
32
31
55
30
29
42
10
14
34
42
52
29
45
14
101
1313
678
1425
826
752
1118
1300
1728
692
743
905
889
770
403
895
Avg.
SD
% CV
33
13
40.97
908
399
43.89
Pall
AcroPrep 96
Competitor M
Pall
AcroPrep 96 Competitor M
4 µg/mL
PE-Ab
58
74
65
126
54
175
38
41
77
108
88
215
168
99
231
179
191
2234
972
1467
894
806
1064
1663
1302
1149
1229
1957
1012
2239
1919
1177
5 µg/mL
PE-Ab
172
209
86
120
119
52
177
108
1307
1194
937
1170
455
696
478
17
1187
1740
2146
1084
4021
1628
2072
767
3584
2842
319
2657
288
656
1053
869
Avg.
SD
% CV
112
63
55.98
1330
556
41.84
Avg.
SD
% CV
456
457
100.18
1682
1129
67.11
The serological immunoassays were performed with two manufacturer’s 96 well filter plates to compare Competitor M filter plates to Pall AcroPrep 96 filter
plates with 1.2 µm Supor® membrane. The serological immunoassays were performed using a single serum sample and three concentrations of rPhycoerythrin
conjugated antibody. The results from these filter plates were read with one Luminex LX100 Instrument. The results presented are for the sixteen individual
responses, the average, the standard deviation, and the percent coefficient of variation for the single serum sample. The Pall Life Sciences AcroPrep 96 filter
plates exhibit a lower level of false positive results than Competitor M filter plates.
Performance (continued)
Figure 2
AcroPrep™ 96 Filter Plates with Prefilter Speeds Sample Processing Times
12
5042
5046
Flow Rate (mL/sec)
10
COMP
8
6
4
2
0
100 µL
200 µL
Volume Filtered
The use of a prefilter shortens filtration times and allows greater flow rates
to be maintained at higher loads. Average flow rates for 100 and 200 µL
lysate samples were calculated for the AcroPrep 96 filter plate with Bio-Inert®
membrane (PN 5042), AcroPrep 96 filter plate with glass fiber over Bio-Inert
membrane (PN 5046), and Competitor hydrophilic PVDF plate (COMP). Error
bar indicates standard error (n = 8).
See Pall Corp. Technical Report PN 33308 for complete experimental details.
Figure 3
AcroPrep 96 UF Filter Plates Remove Salts While Maintaining High Sample Recoveries
AcroPrep 96 30K
AcroPrep 96 10K
100
99.1
97.3
94
93
100
90
90
80
80
70
70
60
60
50
40
50
40
30
30
20
20
10
10
0
99.2
98.1
94
95
0
% Salt Removed
% Protein Recovered
% Salt Removed
Ovalbumin (45kDa) 0.1 mg/mL
500 mM NaCl
BSA (66kDa) 0.1 mg/mL
500 mM NaCl
Ovalbumin (45kDa) 1.0 mg/mL
200 mM NaCl
BSA (66kDa) 1.0 mg/mL
200 mM NaCl
% Protein Recovered
In addition to desalting, ultrafiltration is also useful for the concentration of biomolecules. The efficiency of AcroPrep 96 UF filter plates with Omega™
membrane to remove salts and other small molecules is not at the expense of sample recovery. Using the AcroPrep 96 10K filter plate, recovery of ovalbumin
proteins were greater than 90% (n = 3) with salt removal efficiencies at greater than 95%. Using BSA and a 30K MWCO membrane, similiar results were
observed. A representative experiment is shown.
See Pall Corp. Technical Report PN 33309 for complete experimental details.
www.pall.com/lab
Ordering Information
AcroPrep™ 96 Filter Plates, 350 µL well
AcroPrep 384 Filter Plates, 100 µL well
Part Number
Description
Pkg
Part Number
Description
Pkg
5033
3K Omega™ membrane, natural
10/pack
5076
10K Omega membrane, long tips, natural
10/pack
5034
10K Omega membrane, natural
10/pack
5077
10K Omega membrane, short tips, natural
10/pack
30K Omega membrane, long tips, natural
10/pack
5035
30K Omega membrane, natural
10/pack
5078
5036
100K Omega membrane, natural
10/pack
5079
30K Omega membrane, short tips, natural
10/pack
5045
0.2 µm GHP membrane, natural
10/pack
5080
100K Omega membrane, long tips, natural
10/pack
5030
0.45 µm GHP membrane, natural
10/pack
5081
100K Omega membrane, short tips, natural
10/pack
0.45 µm GHP membrane, long tips, natural
10/pack
10/pack
5043
0.45 µm GHP membrane, white
10/pack
5070
5044
0.45 µm GHP membrane, black
10/pack
5071
0.45 µm GHP membrane, short tips, natural
5037
0.2 µm PTFE membrane, natural ††
10/pack
5072
1.0 µm glass fiber media, long tips, natural
10/pack
5072W
1.0 µm glass fiber media, long tips, white
10/pack
5073
1.0 µm glass fiber media, short tips, natural
10/pack
5073W
1.0 µm glass fiber media, short tips, white
10/pack
5084
1.2 µm Supor membrane, long tips, natural
10/pack
5085
1.2 µm Supor membrane, short tips, natural
10/pack
5038
0.45 µm PTFE membrane, natural ††
10/pack
5042
0.2 µm Bio-Inert® membrane, natural
10/pack
5046
3.0 µm glass fiber media/0.2 µm Bio-Inert
membrane, natural
10/pack
5031
1.0 µm glass fiber, natural †
10/pack
5031L
1.0 µm glass fiber, natural †, with lid
10/pack
5032
1.0 µm glass fiber, white †
10/pack
5029
0.45 µm Supor® membrane, natural
10/pack
5039
1.2 µm Supor membrane, natural
10/pack
5039L
1.2 µm Supor membrane, natural, with lid
10/pack
5041
Prefilter material/1.2 µm Supor membrane,
natural
10/pack
5047
Mustang™ Q ion exchange membrane, natural
5048
7267
Mustang S ion exchange membrane, natural
30-40 µm screen, natural
† Glass Fiber plates have polypropylene support
†† PTFE plates have polyester support
Vacuum Manifold
Part Number
Description
Pkg
5017
Multi-well plate vacuum manifold
1/pkg
10/pack
5014
1 mL receiver plate spacer block
1/pkg
10/pack
5015
350 µL receiver plate spacer block
1/pkg
10/pack
5016
Replacement accessory kit
(includes O-ring, gasket and allen wrench)
1/pkg
Pkg
AcroPrep 96 Filter Plates, 1 mL well
Accessories
Part Number
Description
Pkg
Part Number
Description
5066
3K Omega membrane, natural
5/pack
5225
Adapter collar for centrifugation
2/pkg
5052
0.2 µm GHP membrane, natural
5/pack
5230
Cap mat for incubation
5/pkg
5054
0.45 µm GHP membrane, natural
5/pack
5231
Multi-well plate lids
10/pkg
5055
0.2 µm PTFE membrane, natural ††
5/pack
5056
0.45 µm PTFE membrane, natural ††
5/pack
5051
1.0 µm glass fiber media, natural †
5/pack
5053
3.0 µm glass fiber media/0.2 µm
Bio-Inert membrane, natural
5/pack
5065
1.2 µm Supor membrane, natural,
with valve mats
5/pack
5062
Mustang Q ion exchange membrane, natural
5/pack
5063
Mustang S ion exchange membrane, natural
5/pack
Application by Membrane Type
GHP Membrane
• Cleavage Reactions
• Receptor:Ligand Screens
• Bead- and Resin-based Applications
• Plasmid Lysate Clearing
• HPLC Sample Prep and Clarification
• Metabolic Studies
• Applications that Use Harsh Organics
• Combi-chem Library Screens
• General Sample Prep
PTFE Membrane
• Molecular Synthesis
• Drug Synthesis Reactions
• Bead- and resin-based Applications
Bio-Inert® Membrane
• Drug Binding Studies
• Purification of Oligos from Polyacrylamide Gels
• General Sample Prep
Glass Fiber over Bio-Inert Membrane
• Clarification of Cell Lysates and
Tissue Homogenates
• Preparation of Proteinaceous Solutions
• Prefiltration
Glass Fiber Membrane
• Gross Fractionation
• Lysate Clarification
• Cell-based Assays
• DNA Binding
Omega™ Ultrafiltration Membrane
• Size Exclusion
• Concentrate, Purify, and Desalt Proteins,
Peptides, Oligos, DNA, and RNA
• DNA Clean-up
• Recovery of Proteins, Oligos, and RNA
from Polyacrylamide Gels
Supor® Membrane
• Sample Preparation
• Plasmid Lysate Purification
• Bead- and Resin-based Assays
• Receptor:Ligand Binding Assays
Related Products Available from Pall
• Centrifugal devices for nucleic acid and protein sample
preparation in the following sample volumes:
Device
Sample Volume
Nanosep® Device
up to 0.50 mL
0.5 to 3.5 mL
Microsep™ Device
3 to 15 mL
Macrosep® Device
15 to 60 mL
Jumbosep™ Device
• Nanosep MF (microfiltration) Centrifugal Devices
are available with Bio-Inert or GHP membranes for low
protein binding and high recoveries in applications such
as particulate removal prior to sample analysis (GHP
product is HPLC grade) and removal of precipitates.
• BioTrace™, Biodyne,® and FluoroTrans® Transfer
Membranes offer precise performance and compatibility
with nearly every detection system available.
• AcroWell™ 96 Filter Plates are an excellent platform for
a wide variety of molecular biology detection
applications.
• Pall Chromatography Resins for the purification of
biomolecules and compounds.
• Enchant™ Protein Purification Kits expedite
proteomic sample prep.
References
K.J. Valenzano, W. Miller, J. Kravitz, P. Samama, D.
Fitzpatrick and K. Seeley. 2000. Development of a
Fluorescent Ligand-Binding Assay Using the AcroWell
Filter Plate, J. Biomolecular Screening, 5(6):455-461,
PN 33220.
N. Tang, 2002. Chemiluminescent Detection of Protein
Binding, Genetic Engineering News, 22(8):34.
Complementary Literature
• Product Data, AcroWell 96 Membrane-bottom Filter
Plates, PN 33306
• Technical Report, Automated Purification of
Combinatorial Libraries Using AcroPrep™ 96 Filter Plate
with GHP Membrane, PN 33245
• Protocol, Desalting/Buffer Exchange for Biomolecules
Using the AcroPrep 96 UF Filter Plate, PN 33309
• Protocol, Lysate Clearance for Prokaryotic DNA Isolation
Using the AcroPrep 96 Filter Plate, PN 33308
• Product Brochure, Accelerate Protein Sample
Preparation and Analysis, PN 33411
• Sales Sheet, AcroPrep and AcroWell Multi-well Filter
Plates PN 33287
• Scientific Technical Report, The AcroWell Filter Plate
Minimizes Crosstalk, online
• Protein Sample Preparation and Analysis Application
Manual, PN 33465
www.pall.com/lab
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
800.521.1520
(+)800.PALL.LIFE
734.665.0651
734.913.6114
(USA and Canada)
(Outside USA and Canada)
phone
fax
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8/07, pdf, GN07.1641
PN33296
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