DNA and endotoxin removal using a membrane
adsorber
Thomas Elich, Applications Engineer, EMD Millipore
ABSTRACT
AEX membrane adsorbers are being increasingly employed for removal of
negatively charged trace contaminants in MAb applications. There is also a
growing interest in employing them for DNA and endotoxin clearance.
Studies have been executed to evaluate the binding capacities of model
DNA and endotoxin feeds on a membrane adsorber with a polyallylamine
ligand. The effect of various buffer conditions (conductivities, pH, buffer
components) as well as the presence of competing and non-competing impurities have been evaluated.
The data is expected to provide an improved understanding of DNA and endotoxin clearance using these
membranes and enable identification of optimal conditions for their robust removal.
BIOGRAPHY
Tom Elich, an Applications Engineer for EMD Millipore in Billerica, MA, develops best practice strategies
for launched products and integrated process solutions for biomanufacturing purification. Prior to EMD
Millipore, Tom helped develop and manufacture a clinical trial HIV vaccine at Omnia Biologics, a CMO in
Rockville, MD. Tom earned a Bachelor of Science degree in Chemical Engineering from Northeastern
University, and brings with him a wide range of bioprocess experience.