Suggestions for better papers • What the judges look for in a paper • Why judges reject papers Suggestions for better papers What the judges look for in a paper: 1.Objectives that are clearly stated in the introduction 2. Concise methods that are logical and free from jargon 3.Results that relate to the objectives 4.Conclusions that are justified by the results Organization • • • • • Introduction Materials and methods Results Discussion Conclusion Introduction • • • • Include some background State your objectives Explain why the research is important If your research is similar to something published, explain how your work is different • We often reject papers that are “copies” of published work Materials and Methods • Should be a logical description of what you did • Make sure that a lay person would understand your methods • Explain how your methods will allow you to meet your objectives • Do not list materials used in your project Results • The most important section of your paper (and your presentation) • Should be easy to interpret by all judges • Emphasize the important points of your results (don’t make us guess) Results: Figures • Usually better than tables • Make sure that figures can stand alone • Make sure that the figures clearly indicate something important • Each figure should be referenced in the text of your results Discussion and Conclusion • Should relate to the objectives stated in the introduction • Should be clearly tied to your results and should not go beyond your data Why judges reject papers • Literature searches • Work that is a repeat of something published and does not expand beyond the original work • Poor study design • If we cannot interpret your results • If we do not understand what the figures are supposed to indicate Problems in Study Design • Sample size insufficient to indicate a trend • Samples insufficiently distributed on the x-axis • Samples should usually be randomly drawn and independent of one another Not enough data to indicate a trend 20 19 18 17 16 15 14 13 12 11 10 10 15 20 25 Sample insufficiently distributed on the x-axis 20 19 18 17 16 15 14 13 12 11 10 r2=0.78, F1,4=6.07, P=0.032 10 15 20 25 Balanced Linear Regression x_axisP=0.000 r2=0.89,y_axis F1,38vs =389, 12.0 y_axis 9.0 6.0 3.0 0.0 0.0 2.5 5.0 x_axis 7.5 10.0 Sample insufficient across the x-axis vs x_axis r2=0.01, y_axis F1,38=.07, P=0.841 8.5 y_axis 7.5 6.5 5.5 4.5 6.0 6.5 7.0 x_axis 7.5 8.0 Independent Samples? When are non-independent samples are OK? • Multiple measurements on an individual to track a response • Before and after measurements on subjects • Can be dealt with statistically (repeated measures ANOVA or paired t-tests) Statistical outliers • • • • What do we do with outliers? Do nothing when you can’t justify deleting In regression, conduct “robust regression” Delete if you can justify Examples from previous speakers • To illustrate some common problems 18 Average Nerve Count 16 14 12 Control 10 Pre-Diabetic 8 Diabetic 6 4 2 0 Control Pre-Diabetic Diabetic Figure 1 Density of Epidermal Nerve Fibers (# nerve branches per Millimeter of epidermal border length + Standard Error) 18 Average Nerve Count 16 14 12 10 8 6 4 2 0 Control Pre-Diabetic Diabetic Figure 1 Density of Epidermal Nerve Fibers (# nerve branches per Millimeter of epidermal border length + Standard Error) Rule 2: Avoid Jargon and be careful with abbreviations DIFFERENTIALLY EXPRESSED GENES IDENTIFIED BY SAM Positive Differentially Expressed Genes, 173 Negative Differentially Expressed Genes, 11 Non- Significant Genes, 6647 Positive Differentially Expressed Genes Negative Differentially Expressed Genes Non- Significant Genes (pos 173) (neg 11) (neu. 6647) 17.5 2.5 Total Polyphenol Radical Scavenging Activity Total polyphenol (g/100g dry weight) 2 12.5 1.5 10 7.5 1 5 0.5 Radical Scavenging activity (m mole Trolox/mg dry weight) 15 2.5 0 0 1 Low PP High polyphenol Medium 4 7 10 13 16 19 22 25 28polyphenol 31 34 37 accumulator 40 43 46 49 52 55pp58 Low accumulators accumulators accumulators Genotypes Radical scavenging activity (µ mole Trolox/mg dry leaf powder) and total polyphenol (g/100 g dry leaf powder) in the leaves of 60 sweetpotato genotypes 2.3 y = 0.1303x - 0.2843 r = 0.85 (n= 30) RSA 1.9 1.5 1.1 0.7 0.3 8.0 10.0 12.0 14.0 16.0 18.0 Total Polyphenol Linear correlations between the total polyphenol contents (g/100g dry matter) and radical scavenging activities (RSA; mmol Trolox/g DM) of sweet potato leaves A C B D Photomicrographs A and B show crystals produced by 500 µM melamine and 500 µM cyanuric acid in H20 (sample 11), at 400X magnification and 40X magnification, respectively. Photomicrographs C and D show crystals produced by 500 µM melamine and 500 µM cyanuric acid in artificial urine (Sample 6), at 400X magnification and 40X magnification, respectively. Pellet weights for Trial 3 samples Trial #3 Samples Solution Weight (in grams) Visible Pellet Pellet Weight (in grams) 1 0.8460 No n/d 2 0.6832 No n/d 3 1.0030 No 0.0021 4 0.9370 No 0.0033 5 1.0130 No 0.0029 6 0.8990 Yes 0.0136 7 0.9972 No 0.0100 8 0.8131 No 0.0001 9 0.7569 No 0.0055 10 1.0275 No 0.0010 11 1.0025 Yes 0.0016 •