Bio- molecules
Life
Two or more atoms
bonded together
• Bio-molecules are large molecules that make up
living things.
Carbohydrates
Proteins
(sugars)
Nucleic Acids
Lipids (fats)
PRE- LAB INFORMATION FOR:
ENZYME ACTION LAB
For this lab, we are going to focus on the bio-molecule:
Protein!
A protein is a large, complex polymer composed of
smaller subunits called amino acids.
So what? Why are proteins important????
You are composed of proteins- your skin, hair, eyes
contain proteins….organs, muscles, bones…
(everything that makes you…you.)
• All actions, such as eating, running, and even thinking,
depend on proteins called enzymes.
Bio-molecule: Proteins
There are tens of thousands of different
kinds of proteins, but they are classified into
five groups:
• STRUCTURAL
• STORAGE
• TRANSPORT
• DEFENSIVE
• ENZYMES
• Enzymes are proteins in your body that control the
chemical reactions needed for life.
(breaking stuff down and putting stuff together)
Why is this important???
Without enzymes….
Bio-molecule: Proteins
PRE- LAB INFORMATION FOR:
ENZYME ACTION LAB
There is an enzyme called Catalase found in MOST
organisms.
This enzyme has the ability to break down H O
(which is Hydrogen Peroxide)
2
2
Hydrogen Peroxide is toxic to most
living organisms.
H O is naturally produced in organisms
as a by-product of cellular respiration
(when your cells are making energy).
2
2
Catalase quickly arrives on the scene
to destroy the Hydrogen Peroxide
before it can do too much damage.
Catalase speeds up the process of breaking down the
Hydrogen Peroxide molecule into water and oxygen.
2 H2O2
2 H2O + O2
2 H2O2
2 H2O
2 H2O2
O2
We will be using
CHICKEN LIVERS
which naturally contains the
enzyme protein CATALASE in its
cells.
We are going to measure the rate of this chemical
reaction by measuring the rate of the appearance of
oxygen (O2)
Part A- We will test the rate based on the amount of Catalase present
Part B- We will test the rate based on temperature
Part C- We will test the rate based on pH
YOUR PRE-LAB
ASSIGNMENT MUST BE
READY TO GO WHEN YOU
WALK IN THE ROOM
TUESDAY.
CONSEQUENCES???
Follow your instructions at your table.
You will need something to write with AND your composition book.
When you finish Part A, let me know and I’ll give you instructions
for Part B…..and then Part C