Lab 2 Enzyme Catalysis
Due Date_____________
Flow Chart
Background- Discuss in words the Objectives- Before doing this lab you
should understand:
 the general functions and activities of enzymes
 the relationship between the structure and function of enzymes
 the concept of initial reaction rates of enzymes
 how the concept of free energy relates to enzyme activity
 that changes in temperature, pH, enzyme concentration, and substrate
concentration can affect the initial reaction rates of enzymecatalyzed reactions; and catalyst, catalysis, and catalase

Procedure - Include everything you do. Label each section
DataPage 23 2A Test of Catalase Activity #1
Base line calculation in box - page 23
Exercise 2 C- The Uncatalyzed Rate of H2O2 Decomposition box page 24
Exercise 2D - An Enzyme-Catalyzed Rate of H2O2 Decomposition box page
24
Table 2.1 page 26
Graph 2.1 page 27
Analysis of Data Page 23 2A Test of Catalase Activity #2-3(use the chicken liver to make
fresh catalase) Use potato for the 3rd question
Page 28 -#1-6
Problems- No Problems.
Conclusion or closing remarksSee objectives page 19
After doing this lab you should be able to;


Measure the effects of changes in temperature, pH, enzyme
concentration, and substrate concentration on reaction rates of an
enzyme-catalyzed reaction in a controlled experiment
Explain how environmental factors affect the rate of enzymecatalyzed reactions