Lecture Section IV Outline Paraxial and Intermediate Mesoderm Vocabulary: o Sclerotome: Forms vertebral and rib cartilage o Myotome: Formsmuscles of back, rib cage, and abdomen o Dermamyotome: Forms dermal cells, limb muscle o Syndetome: Formsmost dorsal tendons o Arthrotome: Formsmost central, vertebral joints/discs, proximal ribs The mesoderm forms at the same time as the ectoderm (during gastrulation and neurulation) o Paraxial mesoderm(head mesoderm and somites) Forms structures surrounding the spinal cord: somites and the cartilage derived from them, bone, muscle and dermis. o Intermediate mesoderm Forms the kidneys, gonads, ductwork and the adrenal cortex. o Lateral plate mesoderm and chordamesoderm also form Paraxial Mesoderm 1. Establishment of periodicity a. Periodic somite formation inherent to mesoderm b. Two waves of secreted activity i. FGF from anterior to posterior ii. ??? from posterior to anterior (evidenced by Hairy1 expression) c. FGF and ??? cause delta and notch to form on adjacent cells d. Delta-notch interaction causes Hairy1 expression to remain on e. Where Hairy1 remains on, somite separation occurs 2. Fissure formation (separation) a. Hairy1 expression causes ephrin expression b. Ephrin causes repulsion of anterior part of presomitic mesoderm 3. Epithelialization a. Paraxial mesoderm up until this point is just a mass of closely associated mesenchymal cells b. Cells respond to hairy1 by expressing n-cadherins i. Causes MTE c. Epithelial sheet begins to form on posterior edge 4. Specification a. Specification occurs according to the position along the anteriorposterior line due to proximity to Hox genes 5. Differentiation a. Somites are competent to form all derivative cell types b. Surrounding structures determines fate i. First step is the induction of the sclerotome formation by the notochord 1. ETM migration of cells from the sclerotome surrounds the vertebrae and later forms cartilage, leaving behind the dermamyotome. ii. Next step is segregation of dermamyotome 1. Emergence of dermatome, primaxial and abaxial dermamyotome a. Dermatome: forms back dermis and brown fat b. Primaxial myotome: forms back and intercostal muscles c. Abaxial myotome: forms abdominal muscle, tongue, limbs 2. Myotome proliferation a. Becomes the stem cell population (or committed progenitors) that supports all muscles growth. The bulk of muscle cells come from these cells b. Divides like crazy. Primaxial and abaxial cells get the muscle started. Myotome finishes the job. c. Present for the duration of the organism’s life. 6. Tissue formation a. Myogenesis: Muscle Formation i. Muscles form from the somitic mesoderm 1. Cells from the center of the somite (myotome) exit the cell cycle when an injury to the muscle occurs and differentiates to support. a. Paracrine signals from the neural tube/ notochord induce somitic cells to express MyoD (not until injury) and Myf-5. These are TFs for muscle genes and for the proteins themselves. i. Wnt and Shh b. Myotome cells begin dividing and are now called myoblasts i. FGF c. These myoblasts line up end-to-end and fuse to form multinucleated cells (myotubes). i. Fibronectin, integrins, cadherins/CAM, myogenin. d. Myotubes begin to fuse and mature (fusion with myoblasts [stem cells]) i. Meltrin, interleukin-4 ii. Interestingly, tubes from different species will fuse – no discrimination. e. The mature muscle fiber is now able to begin contracting. b. Osteogenesis: Bone Formation i. Four different sources of cells and two different processes 1. Endochondrial ossification a. Somites form axial skeleton b. Lateral plate mesoderm form limb skeleton 2. Intramembranous ossification a. Cranial NC forms bones of the face and head b. Mesodermal mesenchyme forms the patella and periosteum ii. Endochondrial Ossification Mechanism 1. Shh from the notochord causes cells from the sclerotome to undergo an ETM transition and migrate. a. These cells are now committed chondrocytes 2. When they get to where they are going they form compact nodules in the model of the bone they will form. 3. They then begin proliferating 4. Along the middle, the cells undergo differentiation and grow very large, secreting calcium into their ECM, killing themselves. 5. Calcified, dead chondrocytes receive an Ihh signal and differentiate into osteoblasts. 6. This spreads in both directions. a. Fronts of chondrocytes leading cell death are known as growth plates b. This expansion occurs through puberty 7. As chondrocytes die, signal is sent to supply blood to osteoclasts and osteoblasts iii. Endochondrial Ossification of Vertebrae 1. Sclerotome mesenchymeare attractedby notochordand neural tubesecretions 2. As motor axonsextend towardmuscles, they gothrough sclerotomeportion of the somite and split themrostralto-caudal and connect to muscle on the other side. 3. The caudal end of one thenrecombines with the rostralend of the next to form thebone model and then bone. c. Vascular Replacement in the Dorsal Aorta i. Sclerotome cells migrate to dorsal aorta and replace endothelial and smooth muscle cells 1. Reason why unclear d. The Syndetome: Tendon Formation i. Last layer of cells before sclerotome undergoes ETM transition and leaves the somite; muscle progenitor cells stimulate the syndetome to become tendon instead of cartilage or muscle. 1. Serves as a connection between muscles and bones Intermediate Mesoderm Adult kidney very complex Embryo has an increasing need to filter blood o Intermediate mesoderm forms pronephric (Wolffian) duct, which serves as the organizer. Duct induces three stages of kidney First two transitory, the third persist Pronephros o Pronephric duct forms as part of pronephros Only functional in water-dwelling organisms Mesonephros o Functional in humans and most other organisms Heart beings beating early creating a need for filtration o Further induction occurs from Wolffian Duct and gonadal structure forms o Metanephrosbegins to form Metanephros o Will end up being adult kidney o Formed through reciprocal induction between Wolffian Duct and intermediate mesoderm (mesenchymal) Bud from duct recruits mesenchyme (soluble signals) to condense around it Mesenchymal cells signal back to bud, telling it to become more complex Further mesenchymal recruitment Capped with glomerulus Lateral Mesoderm and Endoderm Vocabulary: o Somatopleure: somatic mesoderm plus ectoderm o Splanchnopleure: splanchnic mesoderm plus endoderm o Coelom: body cavity forms between them Coelom Two cavities exists which eventually fuse Runs from neck to anus in vertebrates Portioned off by folds of somatic mesoderm o Pleural cavity: surrounds the thorax and lungs o Pericardial cavity: surrounds the heart o Peritoneal cavity: surrounds the abdominal organs Heart Development First functional organ o Must switch from diffusion of nutrients to active transport early in development to facilitate growth Three stages of development o Tube formation Specified heart cells start out as heart field in epiblast Migrate through at primitive groove as mesenchyme– condenses into splanchnic mesoderm using cadherins and association with endoderm Outflow guys migrate first and forward Inflow second Two endocardial tubes form on either side of the midline MET of endocardial cells to migrate in between the mesoderm and endoderm o Cardiogenic mesoderm migrations from lateral plate mesoderm At the same time endoderm is folding inward and continues to do so until it forms its own tube, enclosing the two heart tubes (endocardial primordia [surrounded by myocardial progenitors]) o Fuse into one tube Signals to surrounding mesoderm to differentiate into myocardium Tube terminates at a fork on each end because of this fusion Can form multiple arteries and veins this way o Inflow tract – Veins Vitelline veins o Outflow tract – Arteries Pulmonary Systemic o Looping Inflow track loops up to form atria on top of ventricles Driven by more cell division on one side (left) than the other Nodal and Pitx2 determine right vs. left looping and LR asymmetry o Chamber formation Septa separate two atria and two ventricles (grow toward cushion) Valve formation – from myocardium Keep blood from flowing back into chambers it just left The truncus arteriosis becomes septated allowing flow from right ventricle to lungsand the other from left ventricle to the body Embryonic circulatory system o Foramen ovale shunts blood from RA to LA Blood must circulate outside of embryo to become oxygenated Redirection of blood flow at birth o Ductus arteriosus (shunts blood from PV to aorta) undergoes apoptosis o Forman ovale grows closed – signaled by pressure Blood Vessel Development Form independently of the heart Needs to form early to connect with extraembryonic membrane o Extraembryonic membrane fulfills needs of embryo before supporting structures have been developed GI tract Lungs Kidneys Evolution o Mammals still extend vessels to empty yolk sac o Birds and mammals build six aortic arches as if we had gills Fluid dynamics o Organized size variance controls volume o Branching controls speed Vasculogenesis o De novo differentiation of mesoderm into endothelium o Recruits smooth muscle Angiogenesis o Starts from intact blood vessel Growth and remodeling in response to blood flow and recruitment signals Blood Cell Formation o Starts in extraembryonic mesoderm and in large embryonic blood vessels o Blood islands Primitive blood cells form Lymphatic Vessels o Form from jugular vein o Sprouts as lymphatic sacs by angiogenesis o Continues to form secondary drainage system o Major conduit for immune cells Hematopoietic SC o Come from splanchnic mesoderm Development of the Endoderm Digestive tube o Anterior endoderm forms anterior intestinal portal o Posterior endoderm forms posterior intestinal portal o Midgut goes through expansion and contraction to yolk o Each end has ectodermal cap, then forms an entrance Derivatives o Head and neck structures Cranial NC migrate through this endoderm, contributing to the surrounding structures o Gut tube forms esophagus, stomach, SI, LI, rectum o Gut tube buds out to form liver, gall bladder, pancreas Localized Wnt/B-Catenin and retinoic acid cause budding Anterior-Posterior Specification o Hox, cSox2, Pdx1, cdxC, cdxA o Reciprocal induction between endoderm and mesoderm Heart induced liver Liver induced epicardium Pancreas Extraembyronic Membranes Adaptations for development on dry land Four sets of extraembryonic membranes o Somatopleure forms amnion and chorion Amnion: covers embryo to keep it from drying out Chorion: surrounds embryo and control gas exchange o Splanchnopleure forms yolk sac and allantois Yolk sac: surrounds yolk Allantois: space for waste Limb Development Similarities o Symmetry in all four limbs Architectural symmetry Extends to muscles, tendons, cartilage, vessels, nerves Size Growth and development Differences o Rostral-Caudal asymmetry o Left-Right asymmetry Mirror image o Structural polarity Proximal-Distal, Anterior-Posterior, Dorsal-Ventral Hox Genes o Combination of how genes influences what limb structures will form Deletion of genes leads to deletion of structure “Morphogenetic Rules” o Grafting limb buds from one organism can induce limbs in different species Limb Field -> Limb Bud Formation Limb Field o Occurs in mesoderm Anterior to posterior o Forms in a ring – surrounded by cells that will not contribute to limb unless other cells are damaged Peribrachial flank tissue Free limb Shoulder girdle Limb Bud o Begins as a bulge in ectoderm Bulge due to migrations of the somatic mesoderm (bone) and abaxial myotome (muscle) Pronephron seems to cause EMT Proximal-Distal Patterning FGF10 from mesodermal mesenchyme in the sub-ectodermal space signals to overlying ectoderm to form apical ridge (AER) AER communicates back to the mesoderm, influencing development there o Hox genes o Called progress zone (PZ) Anterior-Posterior Patterning Zone of Polarizing Activity o Piece of mesoderm at the junction of the young limb bud and the body wall o When a ZPA is grafted to anterior limb bud mesoderm, o Duplicated digits emerge as a mirror image of the normal digits o Source of soluble signals (Shh) Forms anterior-posterior gradient o Time of expression also factors into patterning o BMP secretions in response to how much Shh and when you saw it Dorsal-Ventral Patterning Determined by overlying ectoderm and Wnt7a (mostly)