WHO STOLE AARON’S IPOD
A ORGANIC COMPOUND MYSTERY LAB
LAB OBJECTIVES
1. Use chemical indicators to determine what
organic compound is in a particular food
2. Accurately collect and record data using tables
3. Determine who stole Aaron’s Ipod using the
chemical composition of a particular food and
comparing it with the evidence food and the
lunch eaten by each of Aaron’s co-workers.
Pretzels
Cheese
Butter
Jelly
Fat-free yogurt
Beans –mashed
FOODS TO BE TESTED
WHAT ORGANIC COMPOUNDS DO YOU THINK THEY CONTAIN?
Test tubes and rack for
Benedict’s Test
Paper bags for the
Lipid Test
Well-plates to hold food for the
Biuret and Iodine Tests
BENEDICT’S
TEST FOR
GLUCOSE
Benedict’s initial color is
blue and will change to
orange if glucose is
present. These tubes
are showing a negative
result.
BROWN BAG LIPID TEST
FOOD WILL BE RUBBED ON BAG WITH EXCESS WIPED OFF AND AIR
DRIED. FOOD WITH TRANSLUCENT GREASE SPOT IS POSITIVE RESULT
FOODS FROM LEFT TO RIGHT ARE YOGURT,
PRETZELS, JELLY, BUTTER, CHEESE AND BEANS
STARTING THE
IODINE AND
BIURET TEST
Examine the two colors
that each indicator can
be.
Iodine – orange
Biuret - blue
FOODS BEING TESTED USING IODINE AND
BIURET SOLUTION. WHAT COLOR SHOWS
A STARCH AND PROTEIN?
Carbohydrate Tests
Food
Protein Test
Lipid Test
Color of
Glucose Color of
Benedict’s Present Iodine
Starch
Present
Color of
Biuret
Protein
Present?
Lipids
Present
blue
no
Black
Yes
Purple
Yes
No
blue
no
Orange
No
Purple
Yes
Yes
blue
no
Orange
No
Blue
No
Yes
orange
yes
Orange
No
Blue
No
no
blue
no
Orange
No
Purple
Yes
No
Beans
blue
(mashed into
a paste)
Evidence 1
blue
no
Black
Yes
Purple
Yes
No
no
Orange
No
Blue
No
yes
Evidence 2
no
Orange
No
Purple
Yes
No
Pretzel
(crumbled
into the
container)
Cheese
Butter
Jelly
Fat – Free
yogurt
blue
NUTRITIONAL INFORMATION
Food
Sugars*
Pretzels
Peanut butter – smooth
Jellies
Low-fat vanilla yogurt+
White beans (canned)
Kidney beans (canned)
Burritos with beans and cheese
White bread
3%
9%
51%
14%
0%
2%
1%
4%
Starch (some
data missing)
71%
5%
0%
9%
5%
41%
Total
carbohydrates
79%
20%
70%
14%
21%
16%
32%
51%
www.nutritiondata.com
Protein
Fat
10%
25%
0%
5%
7%
5%
7%
8%
3%
50%
0%
1%
0%
1%
6%
3%
DATA ANALYSIS
DETERMINE THE ORGANIC COMPOUNDS IN THE FOODS
THAT EACH CO-WORKER A TE
Who stole Aaron’s Ipod?
Worker in
break room
Lunch he or she was eating
Glucose
Starch
Jose
Bean burrito with cheese
x
Ashley
Fat-Free Yogurt
Bruce
Toast with butter and jelly
Kiara
Pretzel
x
Possible
Thief
Evidence 1 and 2
x
Protein
x
Lipid
x
x
x
x
x
x
x
Results for the Lipid Test
POST LAB QUESTIONS
1. Think about Questions 5 and 6 on lab.
2. Complete the table in the lab that summarizes the
evidence and interpretation of the evidence.
3. What happened to Aarons Ipod? Explain
ENERGY IN FOOD
•
•
•
•
•
Energy in food is measured in Calories.
Carbohydrates provide 4 Calories per gram
Proteins provide 4 Calories per gram
Lipids provide 9 Calories per gram
Calculating food Calories
• Read the food label.
• Determine the number of grams per serving for a given
organic compound.
• Multiple the number of grams by the number of calories/g
for the given food.
Let’s practice…
1. What is the total Calories for one serving?
2. What is the number of Calories from Fat?
3. How many Calories come from Protein?
4. How many Calories come from
Carbohydrates1?’
5. Is Sodium an organic compound?
6. Name the two types of fats.