Response to stimuli Contents Introduction Examples of responses Responses in the Flowering Plant Growth regulation Tropism Plant Growth Regulators Commercial use of regulators Plant Protection Auxin Other Roles of Auxin Phototropism and auxin 2 Introduction Living organisms sense and respond to certain environmental changes (stimuli), responding in a variety of ways. Plants and animals respond in complex ways e.g. by growth and movement. Response is a form of defence that allows certain organisms survive. 3 Introduction (cont’d) Some structures of behaviour include: a. Chemical or hormonal system b. Nerve and sense organ system c. Muscular and skeletal systems d. An immune system e.g. response to viral infection. 4 Examples of responses (1/2) In humans: 1. Eye – moving car – image to brain via nerves – message to muscles to move – also message to adrenal glands (adrenaline) – heart beats faster – more oxygen to cells for fast reaction. Above example uses interconnected systems to respond to a stimulus. What was the stimulus? 5 Examples of responses (2/2) 2. If a micro-organism (m/o) enters the body the immune system will respond to it and defend the body in a number of ways and prevent the m/o doing any harm. In plants: 1. Light shining on a shoot from the side causes a chemical release that stimulates the plant to grow towards the direction of the light. 6 Responses in the Flowering Plant Growth regulation Growth of plants is regulated by a number of external factors, which include Light intensity, day (or night) length, gravity and temperature And the principal internal factors that regulate these responses is a group of chemicals called growth regulators – produced in meristematic regions. Growth regulators = auxins. Give e.g. of meristematic regions. 8 Tropism - definition tropism: is the growth response of part of a plant to an external unidirectional stimulus. Positive tropism - plant grows towards stimulus. Negative tropism - plant grows away from stimulus. stimulus: (plural = stimuli): any change in the environment (internal or external) that causes a cell or organism to respond. 9 Plant Tropisms Tropism Growth response of a plant to … Phototropism Growth response of a plant to unidirectional light. Geotropism Growth response of a plant to … Thigmotropism Growth response of a plant to … Hydrotropism Growth response of a plant to … Chemotropism Growth response of a plant to … 10 Significance of phototropism Ensures that a) Plants grow towards the light and b) Leaves face the sun to maximise photosynthesis Demonstrated by placing growing cress seed on a windowsill What will happen? Result– they grow towards the light coming in the window. 11 Significance of geotropism Ensures that a) Roots grow downwards and anchor the plant and b) Stems grow upwards when seeds germinate. 12 Plant Growth Regulators Definition: plant growth regulators: are chemicals produced in plants that affect their rate of growth or development when they are in very low concentrations. They are carried in the vascular system of the plant or diffuse from cell to cell. 13 Types of Plant Growth Regulators Growth promoters e.g. auxins, giberellins and cytokinins Growth inhibitors e.g. ethene (ethylene) and abscisic acid 14 Plant growth regulators 15 Combinations of regulators Sometimes two regulators may work together or against each other e.g. leaf fall (abscission). Ethene is responsible for leaf fall – auxin prevents leaf fall. Young leaves produce auxin – prevents leaf fall. As leaf ages auxin production stops and ethene production increased (due to longer nights). Broad-leaved trees loose their leaves in autumn. 16 Commercial use of regulators Scientists have discovered chemicals that mimic the effect of certain growth regulators and have used these to affect plant growth, e.g. 2-4-D a broad-leaved plant (dicot) weedkiller, it makes the rate of respiration faster than the rate of photosynthesis. Spraying a crop with gibberellins will cause fruit formation without fertilisation and we get the production of seedless fruit. 17 Other examples Ethene: Bananas are picked when green and stored in a warehouse. Ethene released into warehouse and ripens them when they are needed for sale. Over-ripe apples release ethene and causes other apples to ripen – one ‘bad’ apple causes … Used as rooting powders (demonstrate) and Used in tissue culturing. 18 Plant Protection (1/2) Plants have a number of anatomical (structural) and chemical means for protecting themselves e.g. Thorns – on thistles and cacti – prevent plant from being eaten Stinging hairs – on nettles - prevent being eaten. 19 Plant Protection (2/2) Ethene – released from wounds on some plants. Causes production of new cells – form a callus that closes the wound. (Ethene is acting as a growth promoter). Poisons: some plants produce poisons at the edge of leaves that kills insects when they chew them. 20 Auxin A sheath called a coleoptile covers the tip of oat seedlings and other similar plants. It was discovered by experimentation that the tip of the coleoptile produces a chemical that diffuses down the shoot and promotes growth of the shoot. 21 Experiment 1 The coleoptile tip was removed There was no growth until it was replaced 22 Experiment 2 The tip was removed and replaced over half of the coleoptile. It grew only on that side and hence it curved. 23 Experiment 3 The coleoptile tip was removed and placed on a piece of agar. The agar alone was placed on the shoot. The shoot grew in the normal way. 24 Other Experiments Using agar blocks with different concentrations of auxin showed that the higher the concentration of auxin the greater the growth. The blocks were placed on one side of the shoot and caused the shoot to bend. The higher the concentration of auxin the greater the bending (growth). 25 The effect of auxin conc. on a shoot 26 Another effect of Auxin Auxin also affects the strength of cell walls. The higher the concentration of auxin the weaker the cell wall. This allows plant cells to expand more. Shoot grows by increasing the normal elongation process of the cells. 27 Other Roles of Auxins (1/3) Auxin affects growth of roots. (Produced in shoot tips) In very weak concentrations it stimulates root growth very slightly. At concentrations that cause maximum shoot growth it inhibits root growth strongly. Affect of auxins on roots of no consequence in nature. It is thought that abscisic acid is involved with the response of the roots to gravity. 28 Other Roles of Auxins (2/3) Auxins cause apical dominance. apical dominance: the suppression of the growth of the lateral buds by the terminal bud, thus allowing the stem to elongate. Pruning removes apical dominance and produces lots of side shoots. 29 Other Roles of Auxins (3/3) Rooting powder contains auxin. When it is applied to a plant cutting it encourages the production of roots at the cut surface. 30 Phototropism and auxin (1/2) Auxin produced in the shoot tip passes down the shoot. This produces an even concentration of auxin across the shoot resulting in an elongation (growth) on all cells and the shoot grows vertically. 31 Phototropism and auxin (2/2) Light shining on one side of the shoot (unilateral light) results in an increase in auxin concentration on the other side (dark side) of the shoot. (auxin moves away from light). Cells on dark side have weaker cell walls. This promotes a greater cell elongation on the dark side resulting in the shoot growing towards the light. 32 The effect of auxins on a shoot 33 END 34