PHOTOSYNTHESIS Photosynthesis • Anabolic (small molecules combined) • Endergonic (stores energy) • Carbon dioxide (CO2) requiring process that uses light energy (photons) and water (H2O) to produce organic macromolecules (glucose). SUN photons 6CO2 + 6H2O C6H12O6 + 6O2 glucose 2 Question: Where does photosynthesis take place? 3 Plants • Autotrophs – produce their own food (glucose) • Process called photosynthesis • Mainly occurs in the leaves: a. stoma - pores b.mesophyll cells Mesophyll Cell Chloroplast Stoma 4 Stomata (stoma) Pores in a plant’s cuticle through which water vapor and gases (CO2 & O2) are exchanged between the plant and the atmosphere. Stoma Carbon Dioxide (CO2) Guard Cell Oxygen (O2) Guard Cell Found on the underside of leaves 5 Mesophyll Cell of Leaf Nucleus Cell Wall Chloroplast Central Vacuole Photosynthesis occurs in these cells! 6 Chloroplast Organelle where photosynthesis takes place. Stroma Outer Membrane Inner Membrane Thylakoid Granum Thylakoid stacks are connected together 7 copyright cmassengale 8 Function of the Stroma • Light Independent reactions occur here • ATP used to make carbohydrates like glucose • Location of the Calvin Cycle copyright cmassengale 9 Thylakoid Thylakoid Membrane Granum Thylakoid Space Grana make up the inner membrane 10 Question: Why are plants green? 11 Chlorophyll Molecules • Located in the thylakoid membranes • Chlorophyll have Mg+ in the center • Chlorophyll pigments harvest energy (photons) by absorbing certain wavelengths (blue-420 nm and red660 nm are most important) • Plants are green because the green wavelength is reflected, not absorbed. 12 Wavelength of Light (nm) 400 500 600 700 Short wave Long wave (more energy) (less energy) 13 Absorption of Light by Chlorophyll Chlorophyll absorbs blue-violet & red light best Absorption violet blue green yellow wavelength orange red 14 Question: During the fall, what causes the leaves to change colors? 15 Fall Colors • In addition to the chlorophyll pigments, there are other pigments present • During the fall, the green chlorophyll pigments are greatly reduced revealing the other pigments • Carotenoids are pigments that are either red, orange, or yellow 16 Question: What do cells use for energy? 17 Energy for Life on Earth • Sunlight is the ULTIMATE energy for all life on Earth • Plants store energy in the chemical bonds of sugars • Chemical energy is released as ATP during cellular respiration 18 Structure of ATP • ATP stands for adenosine triphosphate • It is composed of the nitrogen base ADENINE, the pentose (5C) sugar RIBOSE, and three PHOSPHATE groups • The LAST phosphate group is bonded with a HIGH ENERGY chemical bond • This bond can be BROKEN to release ENERGY for CELLS to use 19 Removing a Phosphate from ATP Breaking the LAST PHOSPHATE bond from ATP, will --– Release ENERGY for cells to use – Form ADP – Produce a FREE PHOSPHATE GROUP 20 High Energy Phosphate Bond 21 FREE PHOSPHATE can be re-attached to ADP reforming ATP Process called Phosphorylation 22 Parts of Photosynthesis 23 Light Reaction Summary Reactants: • H2O • Light Energy Energy Products: • ATP • NADPH copyright cmassengale 24 Light Independent Reaction • ATP & NADPH from light reactions used as energy • Atmospheric C02 is used to make sugars like glucose and fructose • Six-carbon Sugars made during the Calvin Cycle • Occurs in the stroma copyright cmassengale 25 Two Parts of Photosynthesis Two reactions make up photosynthesis: 1.Light Reaction or Light Dependent Reaction Produces energy from solar power (photons) in the form of ATP and NADPH. SUN 26 Two Parts of Photosynthesis 2. Calvin Cycle or Light Independent Reaction • Also called Carbon Fixation or C3 Fixation • Uses energy (ATP and NADPH) from light reaction to make sugar (glucose). 27 Chloroplast Outer Membrane Inner Membrane STROMA– where Calvin Cycle occurs Thylakoid Granum 28 Photorespiration • • • • Occurs on hot, dry, bright days Stomates close Fixation of O2 instead of CO2 Produces 2-C molecules instead of 3-C sugar molecules • Produces no sugar molecules or no ATP 29 Photorespiration Because of photorespiration, plants have special adaptations to limit the effect of photorespiration: 1. C4 plants 2. CAM plants 30 C4 Plants • Hot, moist environments • 15% of plants (grasses, corn, sugarcane) • Photosynthesis occurs in 2 places • Light reaction mesophyll cells • Calvin cycle - bundle sheath cells 31 CAM Plants • Hot, dry environments • 5% of plants (cactus and ice plants) • Stomates closed during day • Stomates open during the night • Light reaction - occurs during the day • Calvin Cycle - occurs when CO2 is present 32 Question: Why do CAM plants close their stomata during the day? 33 Cam plants close their stomata in the hottest part of the day to conserve water Cellular Respiration copyright cmassengale 35 Cellular Respiration • A catabolic, exergonic, oxygen (O2) requiring process that uses energy extracted from macromolecules (glucose) to produce energy (ATP) and water (H2O). C6H12O6 + 6O2 6CO2 + 6H2O + energy ATP glucose copyright cmassengale 36 Question: • In what kinds organisms does cellular respiration take place? copyright cmassengale 37 Plants and Animals • Plants - Autotrophs: self-producers. • Animals - Heterotrophs: consumers. copyright cmassengale 38 Mitochondria • Organelle where cellular respiration takes place. Outer membrane Inner membrane space Matrix Cristae Inner membrane copyright cmassengale 39