An overview of the
activities of our team
Authors:
•
•
•
•
Rosa Graciela Cohen
Alba Graciela Papeschi
Ximena Inés Pastorino
Francisco Amat
• Lagoon in Comandancia Frías (Chaco Prov.):
24º 30´S, 62º 15´W
• Salinas Grandes (NO Córdoba Prov.):
26º 30´-31º 40´S, 64º 15´-66º 25´W
• Lagoon 40km Northern Los Juríes (Santiago del Estero Prov.):
28º 03´S, 62º 10´W
• La Antigua Saline (La Rioja Prov.):
29º 54´S, 66º 07´W
• Mar Chiquita Lake (Córdoba Prov.):
30º 30´- 31º S, 62º 10´- 63º 10´W
• Pampa de las Salinas (San Luis-La Rioja Prov.):
32º S, 66º 41’ W
• Las Tunas Lagoon (Córdoba Prov.):
33º 44´S, 62º 32´W
• Lagoon in Trenque Lauquen (Buenos Aires Prov.):
35° 48´S, 62° 55´W
• Lagoon in Área de Naicó (La Pampa Prov.):
approximately 36º52´S, 64º 24´W
• Salinas Grandes de Hidalgo (La Pampa Prov.):
37º 13´S, 63º 26´W
• Utracán Lagoon (La Pampa Prov.):
37º 17´14´´S, 64º 37´14´´W
• Guatraché Lagoon (La Pampa Prov.):
37º44´25´´S, 63º 33´15´´W
• El Carrizal Lagoon (La Pampa Prov.):
37º 50´48´´S, 63º 46´54´´W
• Colorada Grande Saline or Lagoon (La Pampa Prov.):
38° 18´S, 63° 42´W
• Colorada Chica Saline or Lagoon (La Pampa Prov.):
38º 23´S, 63º 36´W
• Callaqueo Saline or Lagoon (La Pampa Prov.):
38º 34´S, 63º 32´W
• Salitral de la Vidriera (= de Garnica) (Buenos Aires Prov.):
38° 42´S, 62° 40´W
• Salinas Chicas (Buenos Aires Prov.):
38º 44´S, 62º 57´W
• Lagoon in San Miguel Field (= of Winchel) (Buenos Aires Prov.):
approximately 39ºS, 62º 30´W
• Palos Blancos Saline or Lagoon (Buenos Aires Prov.):
approximately 39ºS, 62º 30´W
• Lagoon in Hotel Las Termas (Pedro Luro, Buenos Aires Prov.):
39° 30´S, 62°35´W
• Villalonga Lagoon (=de los Gauchos) (Buenos Aires Prov.):
39° 51´S, 62° 32´W
• Salitral and Saline of Bajo del Gualicho (Río Negro Prov.):
40º 24´S, 65º 13´W
• Salina de Luzzetti (Buenos Aires Prov.):
approximately 40º 35´S, 62º 40´W
• Salina del Algarrobo (Buenos Aires Prov.):
40° 36´S, 62° 56´W
• Salina de Piedras (= Salina de Cagliero) (Buenos Aires Prov.):
40° 41´S, 62° 40´W
• Saline Pond at the edge of Nat. Route 3, km 1128 (R. Negro Prov.):approximately 40º 43´S, 65º W
• Salina del Inglés (Buenos Aires Prov.):
40° 43´S, 62° 28´W
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MORPHOLOGY
BIOMETRY OF CYSTS AND NAUPLII
MORFOMETRY OF ADULTS
CYTOGENETIC
INTER AND INTRAPOPULATIONAL
CROSS-BREEDING
 LIPID PROFILES
• Lagoon in Comandancia Frías (Chaco Prov.):
24º 30´S, 62º 15´W
• Salinas Grandes (NO Córdoba Prov.):
26º 30´-31º 40´S, 64º 15´-66º 25´W
• Lagoon 40km Northern Los Juríes (Santiago del Estero Prov.):
28º 03´S, 62º 10´W
• La Antigua Saline (La Rioja Prov.):
29º 54´S, 66º 07´W
• Mar Chiquita Lake (Córdoba Prov.):
30º 30´- 31º S, 62º 10´- 63º 10´W
• Pampa de las Salinas (San Luis-La Rioja Prov.):
32º S, 66º 41’ W
• Las Tunas Lagoon (Córdoba Prov.):
33º 44´S, 62º 32´W
• Lagoon in Trenque Lauquen (Buenos Aires Prov.):
35° 48´S, 62° 55´W
• Lagoon in Área de Naicó (La Pampa Prov.):
approximately 36º52´S, 64º 24´W
• Salinas Grandes de Hidalgo (La Pampa Prov.):
37º 13´S, 63º 26´W
• Utracán Lagoon (La Pampa Prov.):
37º 17´14´´S, 64º 37´14´´W
• Guatraché Lagoon (La Pampa Prov.):
37º44´25´´S, 63º 33´15´´W
• El Carrizal Lagoon (La Pampa Prov.):
37º 50´48´´S, 63º 46´54´´W
• Colorada Grande Saline or Lagoon (La Pampa Prov.):
38° 18´S, 63° 42´W
• Colorada Chica Saline or Lagoon (La Pampa Prov.):
38º 23´S, 63º 36´W
• Callaqueo Saline or Lagoon (La Pampa Prov.):
38º 34´S, 63º 32´W
• Salitral de la Vidriera (= de Garnica) (Buenos Aires Prov.):
38° 42´S, 62° 40´W
• Salinas Chicas (Buenos Aires Prov.):
38º 44´S, 62º 57´W
• Lagoon in San Miguel Field (= of Winchel) (Buenos Aires Prov.):
approximately 39ºS, 62º 30´W
• Palos Blancos Saline or Lagoon (Buenos Aires Prov.):
approximately 39ºS, 62º 30´W
• Lagoon in Hotel Las Termas (Pedro Luro, Buenos Aires Prov.):
39° 30´S, 62°35´W
• Villalonga Lagoon (=de los Gauchos) (Buenos Aires Prov.):
39° 51´S, 62° 32´W
• Salitral and Saline of Bajo del Gualicho (Río Negro Prov.):
40º 24´S, 65º 13´W
• Salina de Luzzetti (Buenos Aires Prov.):
approximately 40º 35´S, 62º 40´W
• Salina del Algarrobo (Buenos Aires Prov.):
40° 36´S, 62° 56´W
• Salina de Piedras (= Salina de Cagliero) (Buenos Aires Prov.):
40° 41´S, 62° 40´W
• Saline Pond at the edge of Nat. Route 3, km 1128 (R. Negro Prov.):approximately 40º 43´S, 65º W
• Salina del Inglés (Buenos Aires Prov.):
40° 43´S, 62° 28´W
CHILE
ARGENTINA
Salinas de Cahuil
(Pichilemu)
34° 25’ S
Lagoon in Trenque
Lauquen (Buenos Aires)
35°48´S
A. franciscana
A. persimilis
MORPHOLOGY OF ADULTS
The proportions of the body in both sexes: lengths
and widths of thorax and abdomen
The size of the frontal knob in the antenna of the
male
The presence or not of a characteristic saddle-like
ornamentation on the back of genital segments in the
female.
The shape and size of furcal rami and the number of
setae they bear, in both sexes.
Population of Las Tunas Lagoon
•33° 44’ S latitude
• Assigned to A. franciscana by MORPHOLOGY
OF ADULTS
OTHER TECHNIQUES CARRIED OUT:
• MORPHOMETRY of ADULTS
• CYTOGENETIC STUDIES
•BIOMETRY OF CYSTS
•INTRA AND INTERPOPULATIONAL CROSSES
CYTOGENETIC STUDIES
a) Abnormalities in the meiosis chromosomes
 Heteromorphic bivalents
 Univalents out of plate at metaphase I
 Laggards at anaphase I
b) Nuclei with an unexpected haploid number:
 Telophase I poles with different haploid
chromosome number
 Meiotic nuclei with n = 22, n = 23 and n = 21.
BIOMETRY OF CYSTS
Mean diameter intermediate among
those belonging to several A.
franciscana and A. persimilis
populations.
Intra Populational Crosses
SGH x SGH
SFB x SFB
MCH x MCH
LT x LT
% oviparism
9
20
22
19
% ovoviviparism
91
80
78
81
Ratio ovip/ovoviviparism
0.1
0.25
0.29
0.24
Intra Populational Crosses
SGH x SGH
SFB x SFB
MCH x MCH
LT x LT
% oviparism
9
20
22
19
% ovoviviparism
91
80
78
81
Ratio ovip/ovoviviparism
0.1
0.25
0.29
0.24
Intra Populational Crosses
SGH x SGH
SFB x SFB
MCH x MCH
LT x LT
% oviparism
9
20
22
19
% ovoviviparism
91
80
78
81
Ratio ovip/ovoviviparism
0.1
0.25
0.29
0.24
Inter Populational Crosses
LT x SGH
SGH x LT
MCH x LT
LT x MCH
MCH x SGH
SGH x MCH
% oviparism
73
57
72
72
0
20
% ovoviviparism
27
43
28
28
100
80
Ratio ovip/ovoviviparism
2.7
1.3
2.6
2.6
0
0.25
Inter Populational Crosses
LT x SGH
SGH x LT
MCH x LT
LT x MCH
MCH x SGH
SGH x MCH
% oviparism
73
57
72
72
0
20
% ovoviviparism
27
43
28
28
100
80
Ratio ovip/ovoviviparism
2.7
1.3
2.6
2.6
0
0.25
Inter Populational Crosses
LT x SGH
SGH x LT
MCH x LT
LT x MCH
MCH x SGH
SGH x MCH
% oviparism
73
57
72
72
0
20
% ovoviviparism
27
43
28
28
100
80
Ratio ovip/ovoviviparism
2.7
1.3
2.6
2.6
0
0.25
Inter Populational Crosses
LT x SGH
SGH x LT
MCH x LT
LT x MCH
MCH x SGH
SGH x MCH
% oviparism
73
57
72
72
0
20
% ovoviviparism
27
43
28
28
100
80
Ratio ovip/ovoviviparism
2.7
1.3
2.6
2.6
0
0.25
Viability of the Offspring
LT x LT
MCH x LT
LT x MCH
MCHxMCH
SGHxSGH
LT x SGH
SGH x LT
MCHxSGH
SGHxMCH
SFB xSGH
SGH xSFB
%Viability in
Oviparism*
58
85
96
98
94
0
2
0
0
1
%Viability in
Ovoviviparism**
54
68
59
94
98
0
0
0
0
0
1
*excluded cysts improperly shelled
*excluded dead nauplii + abortive embryos
% viability total
offspring
56
77
78
96
96
0
1
0
0
0
1
Viability of the Offspring
LT x LT
MCH x LT
LT x MCH
MCHxMCH
SGHxSGH
LT x SGH
SGH x LT
MCHxSGH
SGHxMCH
SFB xSGH
SGH xSFB
%Viability in
Oviparism*
58
85
96
98
94
0
2
0
0
1
%Viability in
Ovoviviparism**
54
68
59
94
98
0
0
0
Expected0 High
0
1
*excluded cysts improperly shelled
*excluded dead nauplii + abortive embryos
% viability total
offspring
56
77
78
96
96
0
1
0
viability 0
0
1
Viability of the Offspring
LT x LT
MCH x LT
LT x MCH
MCHxMCH
SGHxSGH
LT x SGH
SGH x LT
MCHxSGH
SGHxMCH
SFB xSGH
SGH xSFB
%Viability in
Oviparism*
58
85
96
98
94
0
2
0
0
1
%Viability in
% viability total
Ovoviviparism**
offspring
54
56
68
77
59
78
94
96
98
96
0 Unexpected Low
0
viability
0
1
0
0
0
0
0
0
1
1
*excluded cysts improperly shelled
*excluded dead nauplii + abortive embryos
Viability of the Offspring
LT x LT
MCH x LT
LT x MCH
MCHxMCH
SGHxSGH
LT x SGH
SGH x LT
MCHxSGH
SGHxMCH
SFB xSGH
SGH xSFB
%Viability in
Oviparism*
58
85
96
98
94
0
2
0
0
1
%Viability in
Ovoviviparism**
54
68
59
94
98
0
0
0
0
0
1
*excluded cysts improperly shelled
*excluded dead nauplii + abortive embryos
% viability total
offspring
56
77
78
96
96
0
1
0
Intermediate
Results
0
0
1
Viability of the Offspring
LT x LT
MCH x LT
LT x MCH
MCHxMCH
SGHxSGH
LT x SGH
SGH x LT
MCHxSGH
SGHxMCH
SFB xSGH
SGH xSFB
%Viability in
Oviparism*
58
85
96
98
94
0
2
0
0
1
%Viability in
% viability total
Ovoviviparism**
offspring
54
56
68
77
Expected Inviability
59
78
94
96
98
96
0
0
0
1
0
0
0
0
0
0
1
1
*excluded cysts improperly shelled
*excluded dead nauplii + abortive embryos
On the basis of our results we have
hypothesized that LT population may be
subjected at least to a certain degree of
natural hybridization. The geographical
proximity of this population with other
populations of both A. franciscana and A.
persimilis, would allow cysts from both
species to converge sporadically in Las
Tunas Lagoon, brought by means of winds or
waterfowls.
CYTOGENETICS STUDIES
Have also been performed in other
populations assigned to A. franciscana.
 Mar Chiquita Lake
 Salinas Grandes (NW Córdoba Prov.)
 Pampa de las Salinas
 La Antigua Saline
In all these cases, the specific
assignation was confirmed, as all the
individuals have shown:
 regular meiosis and n = 21.
 nauplii with 2n = 42
 all interphase nuclei from somatic cells
with numerous DAPI and CMA bright
chromocenters, both in diploid and
polyploid cells.
Thank you for your
attention